Standard name | |
---|---|
Human Ortholog | |
Description | SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 7 | 2 | 3 | 5 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 50 | 42 | 10 | 68 | 58 | 50 | 68 | – | 33 | 54 | 41 | 42 | 48 | 81 | 71 | 36 | 30 | 38 | 27 | 15 | 12 | 19 | 46 | 30 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 2 | 0 | – | 1 | 0 | 0 | 1 | 4 | 0 | 1 | 5 | 5 | 5 | 0 | 0 | 0 | 1 | 2 | 3 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 3 | – | 5 | 2 | 0 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 4 |
Golgi | 1 | 0 | 0 | 0 | 2 | 0 | 2 | – | 0 | 1 | 0 | 0 | 5 | 0 | 3 | 5 | 5 | 5 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 14 | 0 | – | 3 | 8 | 14 | 14 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 25 | 6 | 8 | 48 | 90 | 45 | 114 | – | 71 | 83 | 70 | 104 | 9 | 22 | 18 | 24 | 7 | 5 | 88 | 69 | 48 | 42 | 89 | 70 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 6 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 6 |
Vac/Vac Membrane | 90 | 61 | 29 | 69 | 167 | 71 | 141 | – | 78 | 92 | 66 | 87 | 78 | 114 | 111 | 86 | 51 | 59 | 4 | 8 | 6 | 14 | 27 | 29 |
Unique Cell Count | 131 | 86 | 42 | 148 | 273 | 159 | 296 | 178 | 216 | 174 | 239 | 118 | 187 | 180 | 127 | 82 | 98 | 140 | 103 | 74 | 89 | 204 | 175 | |
Labelled Cell Count | 169 | 109 | 47 | 187 | 317 | 182 | 330 | 194 | 242 | 193 | 258 | 146 | 219 | 204 | 157 | 101 | 114 | 140 | 103 | 74 | 89 | 204 | 175 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.0 | 15.8 | 13.2 | 12.3 | 12.9 | 8.2 | 11.0 | – | 9.5 | 10.0 | 9.0 | 9.9 | 15.6 | 15.9 | 16.3 | 23.8 | 24.0 | 23.2 | 13.8 | 14.2 | 15.3 |
Std Deviation (1e-4) | 2.9 | 2.5 | 3.4 | 2.0 | 1.9 | 2.1 | 1.9 | – | 1.9 | 1.8 | 1.7 | 2.9 | 2.7 | 3.0 | 2.7 | 6.2 | 6.7 | 6.6 | 2.8 | 2.6 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.03 | -0.69 | -0.26 | – | -0.47 | -0.4 | -0.55 | -0.42 | 0.24 | 0.27 | 0.3 | 0.85 | 0.86 | 0.81 | 0.06 | 0.1 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.6 | -0.4 | 1.0 | -0.1 | – | -0.8 | 0.2 | 0 | -1.0 | 2.0 | 2.3 | 1.9 | 0.6 | 1.4 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.7 | 1.8 | 1.2 | 2.5 | – | 2.5 | 2.4 | 2.6 | 3.0 | 0 | -1.3 | 0 | 0 | -1.7 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.6 | -1.0 | -2.8 | -2.6 | – | -2.9 | -3.1 | -3.6 | -4.0 | -0.3 | -1.0 | -0.9 | -0.2 | -0.8 | -1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9549 | 8.3466 | 6.0276 | 6.4548 | 7.0101 | 6.2221 | 8.4503 | 11.6111 | 10.3052 | 7.838 | 8.9502 | 10.088 | 9.0349 | 11.8159 | 10.6988 | 10.9397 | 9.3581 | 10.6989 |
Actin | 0.0172 | 0.0002 | 0.0839 | 0.0011 | 0.0001 | 0.0042 | 0.0034 | 0.0063 | 0.0258 | 0.1604 | 0 | 0.0005 | 0.0423 | 0.0008 | 0.0152 | 0.0001 | 0.0759 | 0.0099 |
Bud | 0.0033 | 0.0001 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0042 | 0.0003 | 0.0021 | 0 | 0.0004 | 0.0005 | 0.0012 | 0.0002 | 0.0001 | 0.0005 | 0.001 |
Bud Neck | 0.0057 | 0.0005 | 0.0028 | 0.0017 | 0.0005 | 0.0012 | 0.0002 | 0.0002 | 0.0002 | 0.0012 | 0.0001 | 0.0025 | 0.001 | 0.0003 | 0.0006 | 0.0003 | 0.0011 | 0.002 |
Bud Periphery | 0.0023 | 0 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0025 | 0.0004 | 0.0036 | 0 | 0.0002 | 0.0007 | 0.0009 | 0.0002 | 0 | 0.0006 | 0.0008 |
Bud Site | 0.1168 | 0.0004 | 0.0031 | 0.0028 | 0 | 0.0003 | 0.0011 | 0.004 | 0.0004 | 0.0021 | 0 | 0.0001 | 0.0009 | 0.0069 | 0.0041 | 0 | 0.0026 | 0.0007 |
Cell Periphery | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0787 | 0.1183 | 0.0447 | 0.0827 | 0.1066 | 0.0648 | 0.1207 | 0.1311 | 0.1954 | 0.0056 | 0.0025 | 0.1007 | 0.0943 | 0.0717 | 0.0975 | 0.0173 | 0.0173 | 0.0758 |
Cytoplasmic Foci | 0.0717 | 0.0003 | 0.0155 | 0.027 | 0.0001 | 0.0058 | 0.0418 | 0.0033 | 0.0037 | 0.0124 | 0 | 0.0003 | 0.0028 | 0.0055 | 0.0031 | 0 | 0.012 | 0.0032 |
Eisosomes | 0.0002 | 0 | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0044 | 0.002 | 0.01 | 0.0024 | 0.0001 | 0.002 | 0.0028 | 0.0005 | 0.0243 | 0.0026 | 0 | 0.002 | 0.0026 | 0.0006 | 0.0028 | 0.0001 | 0.0102 | 0.0042 |
Endosome | 0.0453 | 0.0012 | 0.0159 | 0.0564 | 0 | 0.0213 | 0.0196 | 0.0003 | 0.0082 | 0.0329 | 0 | 0.0012 | 0.0068 | 0.0079 | 0.0089 | 0 | 0.0392 | 0.0286 |
Golgi | 0.0283 | 0 | 0.0057 | 0.0071 | 0 | 0.005 | 0.0029 | 0.0004 | 0.0095 | 0.0446 | 0 | 0 | 0.0064 | 0.0038 | 0.0051 | 0 | 0.0036 | 0.0108 |
Lipid Particles | 0.014 | 0 | 0.0056 | 0.0461 | 0 | 0.0112 | 0.0148 | 0 | 0.0018 | 0.0382 | 0 | 0.0001 | 0.0048 | 0.0002 | 0.0017 | 0 | 0.0107 | 0.0041 |
Mitochondria | 0.012 | 0.0002 | 0.0012 | 0.0017 | 0.0001 | 0.0011 | 0.001 | 0.0279 | 0.0032 | 0.0414 | 0 | 0.0004 | 0.0534 | 0.0015 | 0.0175 | 0.0001 | 0.0014 | 0.004 |
None | 0.0119 | 0.0005 | 0.0037 | 0.0029 | 0.0001 | 0.0009 | 0.009 | 0.0003 | 0.0009 | 0.0007 | 0 | 0.0003 | 0.0009 | 0.0002 | 0.0013 | 0 | 0.0029 | 0.0008 |
Nuclear Periphery | 0.0384 | 0.0252 | 0.0451 | 0.0217 | 0.0081 | 0.0167 | 0.0185 | 0.0177 | 0.0304 | 0.0104 | 0.0047 | 0.0501 | 0.0252 | 0.0072 | 0.0106 | 0.0083 | 0.0273 | 0.0177 |
Nucleolus | 0.0017 | 0.0024 | 0.0006 | 0.0018 | 0.0004 | 0.0007 | 0.0008 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0018 | 0.0016 | 0.0015 | 0.0004 | 0.0004 | 0.0011 | 0.0007 |
Nucleus | 0.4843 | 0.8238 | 0.7425 | 0.7175 | 0.8818 | 0.8559 | 0.7033 | 0.7961 | 0.6759 | 0.6289 | 0.9919 | 0.8325 | 0.7435 | 0.8605 | 0.8247 | 0.9717 | 0.7683 | 0.8245 |
Peroxisomes | 0.0111 | 0 | 0.0061 | 0.004 | 0 | 0.0016 | 0.0142 | 0.0018 | 0.001 | 0.0076 | 0 | 0 | 0.0017 | 0.0003 | 0.0033 | 0 | 0.0029 | 0.0013 |
Punctate Nuclear | 0.0442 | 0.0048 | 0.005 | 0.0113 | 0.0018 | 0.005 | 0.044 | 0.0012 | 0.0015 | 0.0018 | 0.0003 | 0.0048 | 0.0046 | 0.0007 | 0.0012 | 0.0011 | 0.0136 | 0.0063 |
Vacuole | 0.0061 | 0.0064 | 0.0045 | 0.0097 | 0.0001 | 0.0012 | 0.0011 | 0.0013 | 0.0102 | 0.0011 | 0 | 0.0012 | 0.0032 | 0.0214 | 0.0007 | 0.0002 | 0.0067 | 0.0022 |
Vacuole Periphery | 0.0012 | 0.0135 | 0.002 | 0.0013 | 0 | 0.0006 | 0.0005 | 0.0003 | 0.0062 | 0.0014 | 0 | 0.0007 | 0.0024 | 0.0066 | 0.001 | 0.0001 | 0.0017 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 62.8522 | 68.6718 | 63.3107 | 62.5321 | 78.4724 | 79.1033 | 70.486 | 70.2948 | 50.7777 | 79.3305 |
Translational Efficiency | 2.0893 | 2.2629 | 1.6086 | 1.4725 | 1.768 | 1.8558 | 1.7383 | 1.5672 | 2.017 | 1.679 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
79 | 217 | 804 | 74 | 2540 | 1637 | 876 | 616 | 2619 | 1854 | 1680 | 690 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1173.35 | 1374.09 | 1688.31 | 1671.37 | 1170.07 | 1279.31 | 1614.96 | 1592.19 | 1170.17 | 1290.40 | 1650.06 | 1600.68 |
Standard Deviation | 170.16 | 226.22 | 282.34 | 311.80 | 182.69 | 205.34 | 265.43 | 321.25 | 182.33 | 210.11 | 276.10 | 321.18 |
Intensity Change Log 2 | — | 0.227843 | 0.524946 | 0.510398 | — | 0.128771 | 0.464904 | 0.444418 | — | 0.179227 | 0.495279 | 0.477831 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001697 | 0.000633 | 0.000496 | 0.002064 | 0.000121 | 0.000357 | 0.000294 | 0.006151 | 0.000168 | 0.000389 | 0.000391 | 0.005713 |
Bud Neck | 0.009620 | 0.006800 | 0.007500 | 0.016113 | 0.007376 | 0.008170 | 0.006031 | 0.017574 | 0.007443 | 0.008009 | 0.006734 | 0.017417 |
Bud Site | 0.008273 | 0.002005 | 0.002061 | 0.032846 | 0.001651 | 0.004106 | 0.000967 | 0.020113 | 0.001850 | 0.003860 | 0.001490 | 0.021479 |
Cell Periphery | 0.000518 | 0.000178 | 0.000132 | 0.000573 | 0.000147 | 0.000133 | 0.000105 | 0.001861 | 0.000158 | 0.000138 | 0.000118 | 0.001723 |
Cytoplasm | 0.074989 | 0.083702 | 0.026619 | 0.029264 | 0.145044* | 0.134137* | 0.033452 | 0.117642* | 0.142931* | 0.128234* | 0.030182 | 0.108164* |
Cytoplasmic Foci | 0.002838 | 0.000991 | 0.000218 | 0.015050 | 0.000496 | 0.002665 | 0.000117 | 0.010491 | 0.000567 | 0.002469 | 0.000165 | 0.010980 |
Eisosomes | 0.000039 | 0.000014 | 0.000014 | 0.000024 | 0.000007 | 0.000008 | 0.000010 | 0.000469 | 0.000008 | 0.000009 | 0.000012 | 0.000421 |
Endoplasmic Reticulum | 0.013366 | 0.002713 | 0.000997 | 0.001851 | 0.001997 | 0.002762 | 0.000696 | 0.003176 | 0.002340 | 0.002756 | 0.000840 | 0.003034 |
Endosome | 0.004882 | 0.000259 | 0.000097 | 0.094573 | 0.000202 | 0.001927 | 0.000048 | 0.020938 | 0.000343 | 0.001732 | 0.000071 | 0.028835 |
Golgi | 0.001294 | 0.000087 | 0.000055 | 0.108802 | 0.000030 | 0.001126 | 0.000035 | 0.033551 | 0.000068 | 0.001004 | 0.000044 | 0.041621 |
Lipid Particles | 0.000864 | 0.000206 | 0.000099 | 0.000531 | 0.000082 | 0.000292 | 0.000052 | 0.000635 | 0.000105 | 0.000282 | 0.000074 | 0.000624 |
Mitochondria | 0.001163 | 0.000655 | 0.001327 | 0.001746 | 0.000619 | 0.001508 | 0.000929 | 0.014692 | 0.000636 | 0.001408 | 0.001119 | 0.013304 |
Mitotic Spindle | 0.006002 | 0.001858 | 0.004868 | 0.181829* | 0.006265 | 0.008912 | 0.004790 | 0.188895* | 0.006257 | 0.008086 | 0.004827 | 0.188138* |
None | 0.003940 | 0.006159 | 0.003910 | 0.009824 | 0.005318 | 0.006546 | 0.007881 | 0.014349 | 0.005277 | 0.006501 | 0.005981 | 0.013864 |
Nuclear Periphery | 0.001154 | 0.000498 | 0.000624 | 0.000748 | 0.001152 | 0.001333 | 0.000489 | 0.004123 | 0.001152 | 0.001235 | 0.000554 | 0.003761 |
Nuclear Periphery Foci | 0.002473 | 0.000744 | 0.000121 | 0.000464 | 0.000165 | 0.001161 | 0.000058 | 0.015261 | 0.000235 | 0.001112 | 0.000088 | 0.013674 |
Nucleolus | 0.004650 | 0.001435 | 0.000194 | 0.000961 | 0.000288 | 0.000770 | 0.000126 | 0.001100 | 0.000420 | 0.000848 | 0.000159 | 0.001085 |
Nucleus | 0.832088* | 0.884074* | 0.947980* | 0.408039* | 0.825207* | 0.812924* | 0.941812* | 0.485871* | 0.825414* | 0.821252* | 0.944764* | 0.477524* |
Peroxisomes | 0.000241 | 0.000062 | 0.000050 | 0.000361 | 0.000023 | 0.000140 | 0.000015 | 0.000954 | 0.000030 | 0.000131 | 0.000032 | 0.000890 |
Vacuole | 0.028424 | 0.006855 | 0.002597 | 0.067873 | 0.003694 | 0.009544 | 0.002070 | 0.029282 | 0.004440 | 0.009230 | 0.002322 | 0.033421 |
Vacuole Periphery | 0.001485 | 0.000072 | 0.000042 | 0.026466 | 0.000116 | 0.001478 | 0.000026 | 0.012871 | 0.000158 | 0.001313 | 0.000033 | 0.014329 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.16 | 2.04 | 0.77 | -2.98 | -2.71 | -6.29 | -8.09 | -2.21 | -1.78 | -1.65 | -6.17 | -8.64 | -2.33 | -1.88 | -1.62 |
Bud Neck | 0.89 | -1.84 | -3.05 | -3.68 | -2.35 | -1.04 | -4.75 | -8.09 | -6.94 | -5.13 | -1.07 | -7.50 | -8.84 | -7.71 | -4.91 |
Bud Site | 1.79 | 1.27 | -1.29 | -2.99 | -2.58 | -4.09 | -2.17 | -6.95 | -4.59 | -6.49 | -3.81 | -2.93 | -7.44 | -5.36 | -6.15 |
Cell Periphery | 2.03 | 1.78 | 0.16 | -3.89 | -3.39 | 0.07 | -1.46 | -1.97 | -1.97 | -1.84 | 0.26 | -2.40 | -2.08 | -2.10 | -1.85 |
Cytoplasm | -0.16 | 4.02 | 1.75 | 2.28 | -1.93 | 2.27 | 27.55 | 10.16 | 7.76 | -7.98 | 3.12 | 29.91 | 11.00 | 8.01 | -8.71 |
Cytoplasmic Foci | 1.89 | 2.73 | 0.49 | -1.02 | -1.65 | -4.94 | 2.96 | -3.76 | -0.15 | -4.34 | -4.83 | 1.98 | -3.88 | -0.39 | -4.35 |
Eisosomes | 4.31 | 2.36 | -0.56 | -4.58 | -2.84 | -3.81 | -10.45 | -1.46 | -1.42 | -1.24 | -3.21 | -12.99 | -1.53 | -1.49 | -1.20 |
Endoplasmic Reticulum | 4.34 | 5.08 | 4.60 | 1.15 | -2.74 | -5.56 | 5.96 | 0.05 | 3.72 | -3.14 | -3.71 | 7.46 | 1.18 | 3.88 | -3.28 |
Endosome | 1.65 | 1.69 | -0.67 | -1.32 | -1.33 | -3.80 | 5.98 | -2.96 | -1.58 | -3.06 | -3.40 | 2.48 | -3.10 | -2.06 | -3.26 |
Golgi | 1.22 | 1.15 | -0.96 | -1.06 | -1.06 | -2.25 | -7.99 | -2.31 | -1.88 | -2.29 | -2.19 | -2.23 | -2.53 | -2.16 | -2.51 |
Lipid Particles | 3.46 | 3.21 | 0.28 | -3.50 | -3.22 | -5.67 | -4.29 | -9.60 | -6.92 | -8.32 | -5.32 | -4.89 | -9.99 | -7.47 | -8.45 |
Mitochondria | 2.04 | -3.84 | -4.10 | -6.17 | -2.15 | -2.41 | -11.96 | -2.82 | -2.13 | -2.18 | -2.39 | -18.81 | -2.99 | -2.31 | -2.06 |
Mitotic Spindle | 0.56 | -1.04 | -2.42 | -2.89 | -2.13 | -0.35 | -1.27 | -8.89 | -8.77 | -8.18 | -0.12 | -3.59 | -9.29 | -9.24 | -7.86 |
None | -0.23 | -0.14 | -2.63 | -2.89 | -3.05 | -0.78 | -2.70 | -7.86 | -6.76 | -5.23 | -0.85 | -2.49 | -8.36 | -7.26 | -6.89 |
Nuclear Periphery | 3.51 | -0.01 | -2.81 | -4.59 | -3.00 | -1.25 | 0.86 | -7.51 | -6.42 | -7.65 | -0.70 | -1.64 | -8.05 | -7.22 | -7.56 |
Nuclear Periphery Foci | 2.22 | 2.33 | 2.61 | 1.04 | 0.30 | -3.50 | 2.72 | -2.86 | -1.84 | -2.95 | -3.33 | -0.16 | -2.78 | -1.81 | -2.70 |
Nucleolus | 1.02 | 1.17 | 0.95 | -0.54 | -2.89 | -2.25 | -1.25 | -6.56 | -2.35 | -5.65 | -1.57 | -0.29 | -4.87 | -2.39 | -5.79 |
Nucleus | -2.91 | -3.85 | 1.92 | 4.68 | 5.47 | 1.48 | -13.81 | 10.70 | 9.57 | 16.95 | 0.32 | -14.33 | 11.05 | 10.58 | 17.54 |
Peroxisomes | 3.09 | 2.54 | 1.33 | -2.72 | -1.75 | -4.10 | -1.72 | -3.43 | -1.67 | -3.32 | -3.97 | -2.94 | -3.55 | -1.85 | -3.02 |
Vacuole | 3.13 | 2.60 | -0.87 | -4.78 | -4.17 | -5.77 | -4.19 | -11.28 | -9.82 | -9.36 | -4.52 | -5.12 | -11.88 | -10.69 | -10.13 |
Vacuole Periphery | 1.38 | 1.39 | -0.59 | -1.13 | -1.14 | -2.80 | 2.78 | -3.07 | -1.15 | -3.18 | -2.69 | 2.58 | -3.22 | -1.47 | -3.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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