Standard name
Human Ortholog
Description Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.11
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.05 0.06 0.07 0.23 0.33 0.32 – 0.18 0.2 0.18 0.25 – – – 0.13 0.13 0.15 0 0 0 0.15 0.28 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.15 0.05 0.07
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0.05 0.21 0.17 – 0.52 0.46 0.71 0.5 – – – 0.05 0 0 0 0 0 0 0 0
Nucleus 0.8 0.89 0.93 0.81 0.63 0.41 0.41 – 0.44 0.47 0.31 0.38 – – – 0.7 0.7 0.72 0.82 0.82 0.74 0.2 0.15 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.13 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.11
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0.06
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0.11 0.07 0.13
Vac/Vac Membrane 0.06 0.1 0 0.09 0.12 0.19 0.17 – 0.08 0.1 0.08 0.1 – – – 0.2 0.17 0.17 0.09 0.1 0.16 0.23 0.33 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 – – – 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 1 – 2 0 4 1 – – – 0 0 0 0 4 3 0 0 1
Bud Neck 1 0 0 0 0 0 0 – 1 0 0 1 – – – 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 1 1 – 5 1 3 3 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 26 18 10 7 26 47 55 – 28 29 45 34 – – – 29 26 27 8 5 6 1 4 2
Endoplasmic Reticulum 1 0 1 0 0 0 0 – 1 0 0 2 – – – 13 4 1 1 0 1 0 0 0
Endosome 4 3 0 4 2 2 6 – 3 0 1 0 – – – 3 2 2 0 3 1 1 0 1
Golgi 0 0 0 1 0 0 1 – 0 0 0 0 – – – 0 1 0 1 3 0 0 0 0
Mitochondria 45 4 2 3 6 29 30 – 81 68 177 69 – – – 12 4 6 5 1 0 0 0 0
Nucleus 244 330 153 79 71 58 71 – 68 70 78 53 – – – 159 138 133 273 320 142 1 2 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 1 0 – – – 0 0 0 1 0 0 0 0 0
Nucleolus 2 0 0 0 0 0 0 – 1 1 0 0 – – – 0 0 0 0 4 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 2 1 1 – 2 1 0 1 – – – 0 0 1 3 2 2 0 1 1
Vac/Vac Membrane 17 37 2 9 13 27 29 – 13 15 19 14 – – – 46 34 31 29 38 31 1 4 0
Unique Cell Count 305 370 164 98 113 141 174 155 148 248 138 227 197 184 332 389 193 7 15 14
Labelled Cell Count 341 392 168 103 120 165 196 205 185 328 178 262 209 201 332 389 193 7 15 14


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.9 5.7 5.5 5.9 4.7 5.1 – 4.2 4.3 3.8 4.0 – – – 7.2 7.2 7.0 – – –
Std Deviation (1e-4) 1.0 0.9 1.1 1.5 1.5 1.3 1.4 – 1.4 1.4 0.7 0.9 – – – 1.0 1.2 1.2 – – –
Intensity Change (Log2) – – – -0.05 0.03 -0.28 -0.18 – -0.43 -0.4 -0.59 -0.52 – – – 0.33 0.33 0.29 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0.3 4.1 6.1 5.9 – 3.3 3.6 3.5 4.5 – – – 2.2 2.2 2.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 2.7 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 5.0 – 0 0 0 0 – – – 2.1 0 0
Nucleus -3.1 -6.3 -9.8 -10.2 – -9.6 -9.0 -12.4 -10.2 – – – -5.6 -5.6 -5.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 3.7 5.3 4.9 – 3.0 3.5 2.9 3.4 – – – 5.7 5.1 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9739 0.5863 0.7653 0.2454 0.753 0.4578 0.2224 0.6352 0.4625 0.2909 0.0654 0.4548 0.8952 0.7767 0.6614 0.5872 0.373 0.7001
Actin 0.0153 0.0001 0.0087 0 0.0051 0.0013 0.0063 0.0002 0.0001 0.0004 0.0002 0.0001 0.038 0.0001 0.0207 0.0075 0.0001 0.0159
Bud 0.002 0 0.0005 0 0.0006 0.0002 0.0001 0.0001 0 0.0003 0 0 0.0007 0.0009 0.0004 0.0006 0 0.0001
Bud Neck 0.0073 0.0002 0.0055 0.0001 0.0352 0.0015 0.0008 0.0004 0.0002 0.0056 0.0006 0.001 0.0061 0.0002 0.0079 0.0006 0.0013 0.0014
Bud Periphery 0.0034 0 0.0006 0 0.0008 0.0007 0.0002 0.0001 0 0.0006 0 0 0.0013 0.0001 0.0006 0.0008 0 0.0003
Bud Site 0.0153 0.0002 0.0059 0 0.0076 0.0002 0.0002 0.0006 0.0001 0.0111 0.0001 0.0001 0.0062 0.0013 0.0123 0.0151 0.0002 0.0002
Cell Periphery 0.001 0 0.0002 0 0.0003 0.0001 0 0 0 0.0005 0 0 0.0004 0 0.0002 0.0002 0 0.0001
Cytoplasm 0.0163 0.0219 0.0146 0.0039 0.01 0.0026 0.0076 0.0255 0.0081 0.0047 0.0601 0.0068 0.0146 0.0158 0.0113 0.0099 0.0128 0.0089
Cytoplasmic Foci 0.0235 0.0007 0.0205 0.0001 0.0349 0.0054 0.0016 0.0007 0.0004 0.0017 0.0024 0.0003 0.0168 0.0025 0.0028 0.005 0.0003 0.0026
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0 0.0001 0 0 0.0003 0 0.0001 0.0002 0 0.0002
Endoplasmic Reticulum 0.0042 0.0009 0.0022 0.0001 0.0007 0.0008 0.0005 0.0007 0.0004 0.0004 0.0029 0.0002 0.0035 0.0003 0.0005 0.0015 0.0014 0.0013
Endosome 0.0412 0.001 0.0218 0 0.0353 0.006 0.0022 0.0007 0.0005 0.0024 0.002 0.0003 0.0265 0.0002 0.0088 0.0062 0.0016 0.0067
Golgi 0.0105 0 0.0126 0 0.0256 0.0023 0.0006 0 0 0.0003 0 0 0.0122 0 0.0023 0.0022 0 0.0023
Lipid Particles 0.0166 0.0002 0.0179 0 0.013 0.003 0.0043 0.0002 0.0001 0.0006 0.0003 0 0.0104 0.0002 0.0022 0.0066 0.0001 0.0019
Mitochondria 0.0115 0.0003 0.0151 0.0002 0.0091 0.0082 0.0021 0.0004 0.0003 0.0011 0.0003 0.0002 0.0145 0.0003 0.0073 0.0023 0.0003 0.0039
None 0.0325 0.0447 0.026 0.038 0.0488 0.016 0.0182 0.0561 0.0348 0.0121 0.0277 0.0384 0.013 0.0382 0.0255 0.0033 0.0017 0.0133
Nuclear Periphery 0.0235 0.0154 0.0154 0.0021 0.008 0.0126 0.0069 0.009 0.0078 0.0052 0.0196 0.0037 0.0173 0.0053 0.0115 0.0407 0.009 0.0136
Nucleolus 0.0147 0.006 0.0079 0.0042 0.016 0.0134 0.0063 0.0075 0.0072 0.0098 0.0077 0.0122 0.0082 0.0046 0.0044 0.0056 0.0074 0.0077
Nucleus 0.6799 0.8734 0.7806 0.9486 0.6783 0.8934 0.9238 0.8744 0.9237 0.8929 0.8446 0.9198 0.772 0.9156 0.8687 0.878 0.9531 0.8837
Peroxisomes 0.0145 0 0.0076 0 0.0449 0.0028 0.0057 0.0001 0 0.0006 0.0001 0 0.0117 0.0001 0.0062 0.0052 0 0.0026
Punctate Nuclear 0.0375 0.0339 0.0316 0.0027 0.0198 0.0265 0.0113 0.0221 0.0159 0.0467 0.0303 0.0167 0.0207 0.013 0.0028 0.0072 0.0097 0.0293
Vacuole 0.0216 0.0008 0.003 0.0001 0.0047 0.0015 0.0008 0.0009 0.0002 0.0026 0.0008 0.0002 0.0042 0.001 0.0029 0.0009 0.0007 0.0023
Vacuole Periphery 0.0073 0.0002 0.0018 0 0.0012 0.0015 0.0003 0.0002 0.0001 0.0003 0.0003 0.0001 0.0015 0.0002 0.0009 0.0006 0.0002 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2354 5.39 9.5991 9.6152 9.7719 3.0131 10.2185 12.3223 10.1762 10.9632
Translational Efficiency 0.345 0.799 0.2926 0.2652 0.3098 1.0405 0.3432 0.2817 0.3575 0.309

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1894 1008 727 1703 1595 1975 1871 98 3489 2983 2598 1801

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.50 686.49 1035.69 856.74 720.51 744.93 992.72 942.50 681.42 725.18 1004.74 861.41
Standard Deviation 71.03 117.14 215.15 110.05 89.17 100.85 131.31 128.33 87.53 110.16 160.45 112.81
Intensity Change Log 2 — 0.082132 0.675414 0.401751 — 0.048086 0.462368 0.387474 — 0.064314 0.567216 0.394255

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000069 0.000510 0.000732 0.000933 0.000074 0.000236 0.000430 0.001435 0.000071 0.000329 0.000514 0.000960
Bud Neck 0.002820 0.004707 0.012390 0.028111 0.001204 0.005650 0.014709 0.006106 0.002081 0.005331 0.014060 0.026913
Bud Site 0.001606 0.002164 0.000911 0.015386 0.000229 0.002438 0.000914 0.008639 0.000977 0.002345 0.000913 0.015019
Cell Periphery 0.000153 0.000201 0.000059 0.000220 0.000069 0.000141 0.000063 0.000133 0.000115 0.000161 0.000062 0.000215
Cytoplasm 0.093442 0.075702 0.002090 0.066806 0.030300 0.042052 0.003275 0.058788 0.064576 0.053423 0.002944 0.066369
Cytoplasmic Foci 0.008752 0.010766 0.000098 0.002363 0.003056 0.004816 0.000014 0.001205 0.006148 0.006827 0.000038 0.002300
Eisosomes 0.000017 0.000031 0.000033 0.000027 0.000013 0.000017 0.000024 0.000042 0.000015 0.000021 0.000026 0.000027
Endoplasmic Reticulum 0.000742 0.003697 0.003082 0.007225 0.001528 0.002559 0.003523 0.008694 0.001101 0.002944 0.003400 0.007304
Endosome 0.000755 0.001717 0.000134 0.003053 0.000355 0.001591 0.000123 0.001392 0.000572 0.001634 0.000126 0.002963
Golgi 0.000165 0.001147 0.000067 0.002243 0.000075 0.000691 0.000035 0.000380 0.000123 0.000845 0.000044 0.002142
Lipid Particles 0.000727 0.002529 0.000063 0.000522 0.000977 0.001119 0.000035 0.000923 0.000841 0.001595 0.000043 0.000543
Mitochondria 0.001436 0.002270 0.000967 0.006234 0.000476 0.006189 0.000821 0.001447 0.000997 0.004865 0.000862 0.005973
Mitotic Spindle 0.000702 0.001385 0.000185 0.026835 0.001687 0.005254 0.000454 0.087694 0.001152 0.003947 0.000379 0.030146
None 0.015324 0.035928 0.001730 0.007329 0.006813 0.007385 0.001842 0.009105 0.011434 0.017031 0.001811 0.007425
Nuclear Periphery 0.000473 0.000708 0.000171 0.003614 0.000712 0.001827 0.000628 0.001643 0.000582 0.001449 0.000500 0.003506
Nuclear Periphery Foci 0.000271 0.000664 0.000121 0.001378 0.000402 0.000435 0.000042 0.000439 0.000331 0.000512 0.000064 0.001327
Nucleolus 0.003357 0.008284 0.005499 0.001701 0.002961 0.004028 0.002276 0.001138 0.003176 0.005466 0.003178 0.001671
Nucleus 0.866574* 0.840690* 0.970538* 0.808542* 0.947581* 0.906283* 0.969743* 0.788990* 0.903606* 0.884118* 0.969965* 0.807478*
Peroxisomes 0.000778 0.001821 0.000279 0.000719 0.000352 0.001457 0.000061 0.001699 0.000583 0.001580 0.000122 0.000772
Vacuole 0.001615 0.004385 0.000758 0.015705 0.001055 0.004883 0.000919 0.019912 0.001359 0.004715 0.000874 0.015934
Vacuole Periphery 0.000222 0.000691 0.000093 0.001057 0.000080 0.000950 0.000069 0.000195 0.000157 0.000863 0.000076 0.001010

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.42 -13.67 -7.13 -2.26 -0.64 -2.91 -22.21 -7.49 -6.51 -5.48 -4.46 -23.11 -7.96 -4.95 -3.10
Bud Neck -2.47 -10.42 -13.69 -12.08 -6.63 -6.95 -17.17 -7.85 -4.60 2.92 -6.25 -18.82 -14.76 -12.35 -4.88
Bud Site -0.64 1.10 -6.22 -5.47 -6.94 -4.47 -9.05 -4.80 -3.39 -4.21 -2.86 -0.81 -7.23 -6.00 -7.19
Cell Periphery -1.35 3.75 -0.31 1.23 -5.49 -2.96 0.64 -4.71 -0.75 -5.10 -2.13 3.72 -2.22 0.05 -5.80
Cytoplasm 2.90 23.77 7.26 3.21 -18.82 -3.64 11.11 -1.13 0.19 -4.18 3.32 25.32 1.96 -0.98 -18.86
Cytoplasmic Foci -1.36 8.38 7.42 6.66 -2.68 -2.14 6.32 4.18 6.40 -4.12 -0.84 10.40 7.93 8.54 -4.38
Eisosomes -6.24 -8.79 -11.16 -1.38 -0.64 -2.51 -11.80 -7.37 -7.03 -6.25 -5.26 -13.27 -15.04 -9.50 -6.87
Endoplasmic Reticulum -10.22 -12.73 -19.97 -10.29 -12.80 -4.68 -13.16 -7.75 -7.20 -6.48 -11.08 -20.67 -20.33 -14.80 -13.40
Endosome -2.23 2.04 -2.53 0.12 -4.39 -3.25 2.85 -5.05 -0.70 -5.97 -3.52 3.12 -3.59 -0.23 -5.23
Golgi -1.57 1.04 -1.92 0.42 -2.20 -3.19 1.16 -2.20 2.29 -2.89 -2.89 1.79 -2.11 0.05 -2.36
Lipid Particles -3.25 4.93 1.15 3.56 -6.73 -0.55 3.20 -1.36 -1.19 -5.39 -3.08 5.27 1.33 4.65 -11.79
Mitochondria -1.44 0.69 -3.63 -3.04 -5.83 -5.39 -7.83 -4.46 4.03 -2.58 -5.07 -0.17 -5.24 0.98 -5.95
Mitotic Spindle -0.93 0.43 -7.66 -7.18 -7.75 -2.34 0.83 -2.38 -2.20 -2.42 -3.05 0.55 -7.72 -6.50 -7.90
None -4.90 11.02 7.32 7.54 -8.54 -0.38 5.39 -0.09 0.19 -3.03 -3.17 12.01 6.31 7.01 -8.85
Nuclear Periphery -4.34 6.48 -10.18 -9.51 -10.94 -4.29 -0.77 -5.60 -3.17 -5.01 -5.09 -0.70 -10.32 -7.25 -9.43
Nuclear Periphery Foci -3.38 0.18 -3.42 -1.35 -3.05 0.27 1.85 -2.17 -2.76 -4.49 -0.89 2.16 -2.85 -2.27 -4.60
Nucleolus -4.83 -3.13 3.27 7.16 6.81 -1.32 1.19 3.75 4.07 3.04 -3.85 -0.48 4.29 7.22 5.55
Nucleus 2.91 -18.51 5.78 1.80 23.35 7.25 -4.03 7.01 5.60 7.57 3.84 -17.37 12.90 9.35 24.54
Peroxisomes -2.32 1.97 1.84 3.93 0.05 -2.80 3.94 -0.76 1.99 -2.01 -3.12 3.61 1.49 3.97 -1.56
Vacuole -3.87 2.00 -13.84 -11.51 -14.09 -4.98 -2.78 -5.27 -5.09 -5.23 -6.05 -1.27 -14.56 -12.74 -14.40
Vacuole Periphery -1.20 1.70 -2.05 0.43 -3.46 -3.82 0.43 -3.25 3.18 -3.34 -3.49 1.87 -2.81 1.60 -3.65
Standard name
Human Ortholog
Description Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Thi3

Thi3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thi3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available