Standard name | |
---|---|
Human Ortholog | |
Description | Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 1 | 1 |
Bud | 0 | 1 | 2 | 1 | 0 | 4 | 14 | 5 | 3 | 12 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 2 | 2 | 3 | 0 | 3 | 0 | 3 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 109 | 191 | 312 | 216 | 199 | 313 | 353 | 280 | 266 | 381 | 245 | 271 | 303 | 436 | 505 | 216 | 226 | 210 | 161 | 163 | 351 | 324 | 330 | 394 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 6 | 3 | 9 | 10 | 10 | 3 | 1 | 5 | 1 | 2 | 2 |
Endosome | 3 | 0 | 12 | 10 | 7 | 2 | 9 | 3 | 5 | 6 | 1 | 0 | 17 | 5 | 2 | 9 | 7 | 2 | 0 | 1 | 2 | 2 | 4 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 4 |
Mitochondria | 6 | 1 | 7 | 25 | 23 | 118 | 167 | 130 | 126 | 261 | 176 | 157 | 0 | 0 | 1 | 0 | 3 | 3 | 0 | 1 | 4 | 2 | 1 | 6 |
Nucleus | 2 | 5 | 4 | 11 | 10 | 11 | 22 | 20 | 18 | 29 | 18 | 13 | 27 | 34 | 20 | 8 | 3 | 9 | 9 | 25 | 15 | 22 | 15 | 13 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 |
Vac/Vac Membrane | 41 | 5 | 95 | 103 | 56 | 96 | 113 | 120 | 114 | 142 | 79 | 64 | 111 | 128 | 137 | 95 | 103 | 77 | 4 | 6 | 8 | 11 | 13 | 14 |
Unique Cell Count | 145 | 195 | 377 | 311 | 263 | 459 | 547 | 449 | 433 | 663 | 431 | 426 | 401 | 529 | 594 | 286 | 291 | 271 | 186 | 205 | 407 | 380 | 383 | 461 |
Labelled Cell Count | 162 | 205 | 435 | 366 | 296 | 545 | 686 | 562 | 534 | 836 | 522 | 518 | 459 | 612 | 670 | 339 | 357 | 315 | 186 | 205 | 407 | 380 | 383 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 7.3 | 6.3 | 5.8 | 5.9 | 5.3 | 5.0 | 5.3 | 5.0 | 4.8 | 4.5 | 4.6 | 7.7 | 7.7 | 7.3 | 7.8 | 8.0 | 7.5 | 7.5 | 7.8 | 8.1 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.4 | 1.3 | 1.7 | 1.6 | 1.4 | 1.6 | 1.2 | 1.0 | 1.1 | 1.1 | 1.0 | 1.1 | 0.9 | 1.2 | 1.7 | 1.2 | 1.3 | 1.2 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.1 | -0.25 | -0.32 | -0.25 | -0.34 | -0.39 | -0.49 | -0.45 | 0.28 | 0.28 | 0.22 | 0.31 | 0.34 | 0.25 | 0.25 | 0.31 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.1 | -2.2 | -4.8 | -6.1 | -6.5 | -6.7 | -8.3 | -7.9 | -6.1 | -2.5 | -0.1 | 0.9 | -2.3 | -1.6 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | -0.4 | -3.1 | -1.5 | -2.7 | -2.0 | 0 | 0 | 0 | 0.8 | -2.4 | -3.6 | 0 | -0.6 | -2.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.8 | 4.1 | 9.6 | 11.0 | 10.4 | 10.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.2 | 2.3 | 1.4 | 2.7 | 2.9 | 2.7 | 2.9 | 2.7 | 2.0 | 4.0 | 4.0 | 2.3 | 1.7 | 0 | 2.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.3 | -1.1 | -1.5 | -1.6 | 0.5 | 0.4 | -1.4 | -2.4 | -3.6 | 0.8 | -0.3 | -0.8 | 2.3 | 2.9 | 0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.68 | 3.7157 | 3.1628 | 2.9911 | 2.55 | 3.1604 | 1.8452 | 2.0653 | 2.0453 | 1.9719 | 1.5698 | 2.1106 | 1.6324 | 2.132 | 1.6864 | 1.7118 | 1.6493 | 1.5379 |
Actin | 0.0003 | 0.0011 | 0.0006 | 0.0012 | 0.0002 | 0.0003 | 0.0554 | 0.0009 | 0.0113 | 0.0551 | 0.0276 | 0.0302 | 0.0371 | 0.0021 | 0.0084 | 0.0004 | 0.0009 | 0.0044 |
Bud | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0083 | 0.0065 | 0.0004 | 0.0011 | 0.0009 | 0.0003 | 0.0061 | 0.0009 | 0.0002 | 0.0001 | 0.0002 |
Bud Neck | 0.0002 | 0.0004 | 0.0003 | 0.0006 | 0.0012 | 0.0017 | 0.0028 | 0.0004 | 0.0019 | 0.0019 | 0.0028 | 0.003 | 0.0018 | 0.0003 | 0.001 | 0.0004 | 0.0009 | 0.0017 |
Bud Periphery | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0009 | 0.0016 | 0.0035 | 0.0005 | 0.0011 | 0.0012 | 0.0003 | 0.0019 | 0.0012 | 0 | 0 | 0.0001 |
Bud Site | 0.0002 | 0.0014 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0069 | 0.008 | 0.0052 | 0.0022 | 0.0039 | 0.0016 | 0.0009 | 0.003 | 0.0005 | 0.0002 | 0.0003 | 0.0002 |
Cell Periphery | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.7125 | 0.7409 | 0.7132 | 0.7447 | 0.6549 | 0.7054 | 0.6095 | 0.8326 | 0.7274 | 0.6832 | 0.6232 | 0.7165 | 0.7275 | 0.8109 | 0.7296 | 0.6708 | 0.6672 | 0.7683 |
Cytoplasmic Foci | 0.0039 | 0.0047 | 0.0045 | 0.005 | 0.0031 | 0.0146 | 0.0521 | 0.0077 | 0.0267 | 0.0477 | 0.0483 | 0.0251 | 0.0111 | 0.0065 | 0.0085 | 0.0039 | 0.0208 | 0.007 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0035 | 0.0035 | 0.0049 | 0.0044 | 0.0019 | 0.0031 | 0.0057 | 0.0037 | 0.0039 | 0.0023 | 0.0025 | 0.0046 | 0.0057 | 0.0035 | 0.0062 | 0.0025 | 0.0041 | 0.0037 |
Endosome | 0.0014 | 0.002 | 0.0033 | 0.0035 | 0.0007 | 0.0045 | 0.0421 | 0.003 | 0.0137 | 0.0106 | 0.0209 | 0.0103 | 0.0173 | 0.0013 | 0.0074 | 0.0011 | 0.0118 | 0.0057 |
Golgi | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0079 | 0.0003 | 0.0093 | 0.0071 | 0.0251 | 0.0038 | 0.0054 | 0.0002 | 0.0012 | 0.0001 | 0.0003 | 0.0006 |
Lipid Particles | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.016 | 0.0002 | 0.0076 | 0.01 | 0.0208 | 0.0042 | 0.0085 | 0.0002 | 0.0027 | 0 | 0.0002 | 0.0003 |
Mitochondria | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0003 | 0.0057 | 0.0007 | 0.0049 | 0.0016 | 0.0516 | 0.0029 | 0.0022 | 0.0005 | 0.0014 | 0.0003 | 0.0003 | 0.0005 |
None | 0.2639 | 0.1791 | 0.2338 | 0.1831 | 0.3055 | 0.2132 | 0.1024 | 0.0899 | 0.1034 | 0.0308 | 0.0325 | 0.0863 | 0.0743 | 0.1069 | 0.1537 | 0.1845 | 0.1709 | 0.091 |
Nuclear Periphery | 0.0016 | 0.0034 | 0.0075 | 0.0046 | 0.0008 | 0.0024 | 0.014 | 0.0032 | 0.0038 | 0.0043 | 0.0047 | 0.0061 | 0.0097 | 0.0032 | 0.0056 | 0.0037 | 0.0074 | 0.0062 |
Nucleolus | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0003 | 0.0084 | 0.0015 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0007 |
Nucleus | 0.0098 | 0.0512 | 0.0263 | 0.0456 | 0.0285 | 0.047 | 0.0343 | 0.0338 | 0.0463 | 0.078 | 0.0696 | 0.0739 | 0.0636 | 0.0484 | 0.0597 | 0.1287 | 0.0832 | 0.0977 |
Peroxisomes | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0227 | 0.0004 | 0.0175 | 0.0522 | 0.0451 | 0.0042 | 0.0204 | 0.0002 | 0.0066 | 0.0001 | 0.0003 | 0.0003 |
Punctate Nuclear | 0.0013 | 0.0095 | 0.0027 | 0.0047 | 0.0021 | 0.0045 | 0.0132 | 0.0031 | 0.0048 | 0.0104 | 0.0064 | 0.0218 | 0.0104 | 0.0033 | 0.0035 | 0.0023 | 0.0284 | 0.01 |
Vacuole | 0.0009 | 0.001 | 0.0011 | 0.0009 | 0.0003 | 0.0008 | 0.0044 | 0.0014 | 0.0014 | 0.0009 | 0.0019 | 0.0013 | 0.0019 | 0.0009 | 0.0011 | 0.0005 | 0.0017 | 0.001 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0017 | 0.0002 | 0.0004 | 0.0002 | 0.0019 | 0.0004 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.2167 | 8.372 | 11.86 | 15.3745 | 14.3503 | 12.7638 | 10.5812 | 13.5041 | 13.4202 | 16.2951 |
Translational Efficiency | 0.8512 | 0.7639 | 0.7986 | 0.7751 | 0.7112 | 0.844 | 0.9199 | 0.6686 | 1.0159 | 0.7641 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1724 | 708 | 2533 | 1375 | 2402 | 991 | 604 | 722 | 4126 | 1699 | 3137 | 2097 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 783.84 | 914.72 | 958.44 | 969.37 | 781.05 | 849.28 | 927.65 | 957.59 | 782.22 | 876.55 | 952.51 | 965.31 |
Standard Deviation | 89.85 | 114.03 | 117.12 | 131.90 | 94.12 | 109.66 | 174.77 | 137.19 | 92.37 | 116.07 | 130.78 | 133.86 |
Intensity Change Log 2 | — | 0.222771 | 0.290129 | 0.306488 | — | 0.120825 | 0.248166 | 0.293993 | — | 0.172789 | 0.269337 | 0.300265 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000104 | 0.000323 | 0.000133 | 0.002801 | 0.000063 | 0.000164 | 0.001713 | 0.001135 | 0.000080 | 0.000231 | 0.000437 | 0.002227 |
Bud Neck | 0.004452 | 0.003814 | 0.004622 | 0.016609 | 0.002996 | 0.004969 | 0.003474 | 0.003756 | 0.003604 | 0.004487 | 0.004401 | 0.012184 |
Bud Site | 0.002050 | 0.005527 | 0.003209 | 0.038557 | 0.001470 | 0.005087 | 0.006486 | 0.017232 | 0.001712 | 0.005270 | 0.003840 | 0.031215 |
Cell Periphery | 0.000092 | 0.000108 | 0.000087 | 0.000218 | 0.000057 | 0.000120 | 0.000180 | 0.000189 | 0.000072 | 0.000115 | 0.000105 | 0.000208 |
Cytoplasm | 0.546780* | 0.614283* | 0.415194* | 0.348945* | 0.578456* | 0.558660* | 0.404358* | 0.416131* | 0.565221* | 0.581839* | 0.413107* | 0.372077* |
Cytoplasmic Foci | 0.129696 | 0.057514 | 0.004309 | 0.007492 | 0.056739 | 0.090813 | 0.008843 | 0.008505 | 0.087223 | 0.076937 | 0.005182 | 0.007841 |
Eisosomes | 0.000061 | 0.000036 | 0.000013 | 0.000044 | 0.000025 | 0.000050 | 0.000039 | 0.000035 | 0.000040 | 0.000044 | 0.000018 | 0.000041 |
Endoplasmic Reticulum | 0.001560 | 0.001827 | 0.001309 | 0.005675 | 0.000981 | 0.001398 | 0.004238 | 0.004349 | 0.001223 | 0.001577 | 0.001873 | 0.005218 |
Endosome | 0.003554 | 0.004632 | 0.000611 | 0.008533 | 0.001386 | 0.007090 | 0.001552 | 0.007160 | 0.002292 | 0.006066 | 0.000792 | 0.008060 |
Golgi | 0.000796 | 0.001916 | 0.000037 | 0.003964 | 0.000358 | 0.001494 | 0.000101 | 0.004291 | 0.000541 | 0.001670 | 0.000050 | 0.004076 |
Lipid Particles | 0.003163 | 0.001427 | 0.000340 | 0.001313 | 0.001356 | 0.002693 | 0.001065 | 0.001540 | 0.002111 | 0.002165 | 0.000480 | 0.001391 |
Mitochondria | 0.002836 | 0.001238 | 0.000609 | 0.015280 | 0.001659 | 0.001840 | 0.000381 | 0.007435 | 0.002151 | 0.001589 | 0.000565 | 0.012579 |
Mitotic Spindle | 0.003008 | 0.007452 | 0.004534 | 0.057297 | 0.002549 | 0.004732 | 0.009857 | 0.020332 | 0.002741 | 0.005866 | 0.005559 | 0.044570 |
None | 0.002383 | 0.004951 | 0.006285 | 0.006009 | 0.003336 | 0.004946 | 0.011468 | 0.009085 | 0.002938 | 0.004948 | 0.007283 | 0.007068 |
Nuclear Periphery | 0.000504 | 0.000476 | 0.001222 | 0.002258 | 0.000537 | 0.000559 | 0.001106 | 0.001609 | 0.000523 | 0.000525 | 0.001200 | 0.002035 |
Nuclear Periphery Foci | 0.000799 | 0.002543 | 0.000271 | 0.001951 | 0.000540 | 0.001302 | 0.002457 | 0.003414 | 0.000648 | 0.001819 | 0.000692 | 0.002455 |
Nucleolus | 0.000740 | 0.000593 | 0.000332 | 0.000383 | 0.000612 | 0.001328 | 0.000599 | 0.000473 | 0.000666 | 0.001021 | 0.000383 | 0.000414 |
Nucleus | 0.255658* | 0.242698* | 0.537140* | 0.357183* | 0.327819* | 0.253866* | 0.490716* | 0.395314* | 0.297667* | 0.249212* | 0.528202* | 0.370311* |
Peroxisomes | 0.001778 | 0.001450 | 0.000253 | 0.003247 | 0.000630 | 0.003372 | 0.000336 | 0.001424 | 0.001110 | 0.002571 | 0.000269 | 0.002619 |
Vacuole | 0.039586 | 0.046592 | 0.019366 | 0.115197 | 0.018280 | 0.054436 | 0.050919 | 0.087706 | 0.027183 | 0.051167 | 0.025441 | 0.105732 |
Vacuole Periphery | 0.000398 | 0.000600 | 0.000122 | 0.007046 | 0.000153 | 0.001080 | 0.000114 | 0.008886 | 0.000256 | 0.000880 | 0.000121 | 0.007679 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.88 | -3.14 | -5.18 | -3.87 | -4.73 | -5.55 | -1.62 | -6.85 | -5.78 | 0.79 | -6.97 | -2.07 | -6.97 | -5.69 | -2.55 |
Bud Neck | 0.70 | -1.63 | -5.16 | -4.74 | -3.91 | -2.25 | -0.65 | -4.75 | -1.40 | -2.70 | -1.30 | -2.97 | -7.14 | -4.73 | -4.29 |
Bud Site | -4.03 | -2.79 | -6.90 | -4.56 | -5.80 | -4.03 | -2.60 | -5.17 | -2.74 | -2.31 | -5.62 | -4.02 | -8.51 | -5.42 | -6.45 |
Cell Periphery | -2.31 | 1.16 | -7.66 | -4.12 | -8.28 | -8.60 | -10.50 | -13.55 | -5.08 | 0.03 | -7.92 | -6.84 | -14.58 | -6.02 | -8.70 |
Cytoplasm | -4.87 | 22.62 | 33.79 | 29.57 | 13.19 | 2.82 | 22.31 | 28.38 | 20.97 | 1.50 | -0.86 | 34.17 | 46.69 | 36.43 | 12.33 |
Cytoplasmic Foci | 13.98 | 33.45 | 33.32 | 15.77 | -1.03 | -6.99 | 21.53 | 22.20 | 19.08 | 0.67 | 2.67 | 39.29 | 38.99 | 24.40 | -1.23 |
Eisosomes | 5.91 | 16.92 | 3.70 | -3.33 | -17.44 | -9.55 | -9.18 | -12.44 | -0.82 | 0.33 | -3.09 | 9.61 | -6.54 | -1.99 | -16.28 |
Endoplasmic Reticulum | -1.05 | -1.33 | -7.89 | -10.81 | -11.35 | -2.47 | -13.12 | -12.37 | -10.49 | 2.02 | -2.21 | -7.82 | -15.12 | -15.48 | -9.95 |
Endosome | -1.34 | 11.14 | 2.48 | 2.77 | -3.45 | -8.55 | -2.24 | -2.06 | 4.92 | -1.35 | -7.47 | 9.87 | -0.23 | 5.97 | -4.10 |
Golgi | -1.09 | 2.39 | 0.12 | 1.20 | -3.29 | -1.70 | 2.19 | -0.88 | 0.51 | -1.29 | -1.97 | 3.28 | -0.90 | 1.29 | -2.91 |
Lipid Particles | 5.20 | 9.57 | 5.48 | -0.46 | -15.98 | -4.69 | -0.30 | -2.35 | 3.18 | -2.06 | -0.57 | 10.25 | 3.00 | 3.22 | -14.10 |
Mitochondria | 1.64 | 2.15 | -0.99 | -2.92 | -3.48 | -0.14 | 2.06 | -0.44 | -0.31 | -1.92 | 0.95 | 2.76 | -1.51 | -2.40 | -3.90 |
Mitotic Spindle | -1.74 | -1.81 | -7.32 | -4.93 | -6.45 | -1.55 | -2.13 | -4.29 | -3.07 | -1.36 | -2.33 | -3.01 | -8.63 | -6.30 | -6.54 |
None | -2.91 | -6.07 | -9.27 | -3.03 | -2.48 | -1.34 | -4.99 | -5.85 | -1.41 | 1.32 | -2.53 | -7.29 | -10.97 | -2.89 | -1.67 |
Nuclear Periphery | -0.15 | -19.21 | -22.66 | -19.47 | -12.96 | -0.63 | -11.97 | -14.93 | -15.97 | -4.40 | -0.57 | -20.48 | -26.18 | -26.02 | -13.76 |
Nuclear Periphery Foci | -5.58 | 5.33 | -6.29 | 3.45 | -10.12 | -4.72 | -6.98 | -4.21 | -2.93 | -1.02 | -7.24 | -2.40 | -6.16 | -0.66 | -5.27 |
Nucleolus | 0.95 | 2.74 | 2.66 | 1.81 | -0.49 | -2.17 | 0.81 | 1.68 | 3.28 | 1.01 | -1.65 | 2.80 | 2.95 | 3.67 | 0.02 |
Nucleus | 1.15 | -42.71 | -22.48 | -19.06 | 13.26 | 8.43 | -16.32 | -9.69 | -14.65 | 6.45 | 7.33 | -43.77 | -21.77 | -24.51 | 15.43 |
Peroxisomes | 1.03 | 5.13 | 2.63 | 1.67 | -1.18 | -3.64 | 2.24 | 0.75 | 3.78 | -0.84 | -2.95 | 5.37 | 2.01 | 3.85 | -1.38 |
Vacuole | -3.62 | 3.81 | -24.89 | -19.27 | -26.57 | -12.01 | -12.63 | -21.46 | -15.39 | -9.20 | -11.35 | -8.79 | -34.17 | -25.01 | -29.17 |
Vacuole Periphery | -1.06 | 3.83 | -1.97 | -1.57 | -2.56 | -2.67 | 2.67 | -1.46 | -0.68 | -1.49 | -2.86 | 3.78 | -2.49 | -1.27 | -2.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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