Standard name
Human Ortholog
Description Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.75 0.98 0.83 0.69 0.76 0.68 0.65 0.62 0.61 0.57 0.57 0.64 0.76 0.82 0.85 0.76 0.78 0.77 0.87 0.8 0.86 0.85 0.86 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.09 0.26 0.31 0.29 0.29 0.39 0.41 0.37 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0.05 0.12 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0 0.25 0.33 0.21 0.21 0.21 0.27 0.26 0.21 0.18 0.15 0.28 0.24 0.23 0.33 0.35 0.28 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3 1 1 1
Bud 0 1 2 1 0 4 14 5 3 12 1 4 0 0 0 0 0 0 0 0 2 2 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 3
Bud Site 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 6 2 2 3 0 3 0 3 2 2 2 2 0 0 0 0 0 0
Cytoplasm 109 191 312 216 199 313 353 280 266 381 245 271 303 436 505 216 226 210 161 163 351 324 330 394
Endoplasmic Reticulum 0 2 1 0 1 0 0 0 0 0 1 3 0 6 3 9 10 10 3 1 5 1 2 2
Endosome 3 0 12 10 7 2 9 3 5 6 1 0 17 5 2 9 7 2 0 1 2 2 4 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 2 0 0 0 1 2 4
Mitochondria 6 1 7 25 23 118 167 130 126 261 176 157 0 0 1 0 3 3 0 1 4 2 1 6
Nucleus 2 5 4 11 10 11 22 20 18 29 18 13 27 34 20 8 3 9 9 25 15 22 15 13
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 2 0 1 1
Nucleolus 1 0 2 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 3
Vac/Vac Membrane 41 5 95 103 56 96 113 120 114 142 79 64 111 128 137 95 103 77 4 6 8 11 13 14
Unique Cell Count 145 195 377 311 263 459 547 449 433 663 431 426 401 529 594 286 291 271 186 205 407 380 383 461
Labelled Cell Count 162 205 435 366 296 545 686 562 534 836 522 518 459 612 670 339 357 315 186 205 407 380 383 461


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.3 6.3 5.8 5.9 5.3 5.0 5.3 5.0 4.8 4.5 4.6 7.7 7.7 7.3 7.8 8.0 7.5 7.5 7.8 8.1
Std Deviation (1e-4) 0.9 1.0 1.4 1.3 1.7 1.6 1.4 1.6 1.2 1.0 1.1 1.1 1.0 1.1 0.9 1.2 1.7 1.2 1.3 1.2 1.2
Intensity Change (Log2) – – – -0.13 -0.1 -0.25 -0.32 -0.25 -0.34 -0.39 -0.49 -0.45 0.28 0.28 0.22 0.31 0.34 0.25 0.25 0.31 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.1 -2.2 -4.8 -6.1 -6.5 -6.7 -8.3 -7.9 -6.1 -2.5 -0.1 0.9 -2.3 -1.6 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 -0.4 -3.1 -1.5 -2.7 -2.0 0 0 0 0.8 -2.4 -3.6 0 -0.6 -2.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.8 4.1 9.6 11.0 10.4 10.4 0 0 0 0 0 0 0 0 0
Nucleus 2.2 2.3 1.4 2.7 2.9 2.7 2.9 2.7 2.0 4.0 4.0 2.3 1.7 0 2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.3 -1.1 -1.5 -1.6 0.5 0.4 -1.4 -2.4 -3.6 0.8 -0.3 -0.8 2.3 2.9 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.68 3.7157 3.1628 2.9911 2.55 3.1604 1.8452 2.0653 2.0453 1.9719 1.5698 2.1106 1.6324 2.132 1.6864 1.7118 1.6493 1.5379
Actin 0.0003 0.0011 0.0006 0.0012 0.0002 0.0003 0.0554 0.0009 0.0113 0.0551 0.0276 0.0302 0.0371 0.0021 0.0084 0.0004 0.0009 0.0044
Bud 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0008 0.0083 0.0065 0.0004 0.0011 0.0009 0.0003 0.0061 0.0009 0.0002 0.0001 0.0002
Bud Neck 0.0002 0.0004 0.0003 0.0006 0.0012 0.0017 0.0028 0.0004 0.0019 0.0019 0.0028 0.003 0.0018 0.0003 0.001 0.0004 0.0009 0.0017
Bud Periphery 0 0.0001 0 0.0001 0 0 0.0009 0.0016 0.0035 0.0005 0.0011 0.0012 0.0003 0.0019 0.0012 0 0 0.0001
Bud Site 0.0002 0.0014 0.0004 0.0003 0.0001 0.0002 0.0069 0.008 0.0052 0.0022 0.0039 0.0016 0.0009 0.003 0.0005 0.0002 0.0003 0.0002
Cell Periphery 0 0.0001 0.0001 0 0 0 0.0002 0.0002 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.7125 0.7409 0.7132 0.7447 0.6549 0.7054 0.6095 0.8326 0.7274 0.6832 0.6232 0.7165 0.7275 0.8109 0.7296 0.6708 0.6672 0.7683
Cytoplasmic Foci 0.0039 0.0047 0.0045 0.005 0.0031 0.0146 0.0521 0.0077 0.0267 0.0477 0.0483 0.0251 0.0111 0.0065 0.0085 0.0039 0.0208 0.007
Eisosomes 0 0 0.0001 0 0 0 0.0006 0 0.0002 0.0001 0.0003 0.0001 0.0003 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0035 0.0035 0.0049 0.0044 0.0019 0.0031 0.0057 0.0037 0.0039 0.0023 0.0025 0.0046 0.0057 0.0035 0.0062 0.0025 0.0041 0.0037
Endosome 0.0014 0.002 0.0033 0.0035 0.0007 0.0045 0.0421 0.003 0.0137 0.0106 0.0209 0.0103 0.0173 0.0013 0.0074 0.0011 0.0118 0.0057
Golgi 0.0001 0.0002 0.0001 0.0002 0 0.0002 0.0079 0.0003 0.0093 0.0071 0.0251 0.0038 0.0054 0.0002 0.0012 0.0001 0.0003 0.0006
Lipid Particles 0.0001 0.0001 0.0002 0.0001 0.0001 0.0003 0.016 0.0002 0.0076 0.01 0.0208 0.0042 0.0085 0.0002 0.0027 0 0.0002 0.0003
Mitochondria 0.0001 0.0003 0.0003 0.0004 0.0001 0.0003 0.0057 0.0007 0.0049 0.0016 0.0516 0.0029 0.0022 0.0005 0.0014 0.0003 0.0003 0.0005
None 0.2639 0.1791 0.2338 0.1831 0.3055 0.2132 0.1024 0.0899 0.1034 0.0308 0.0325 0.0863 0.0743 0.1069 0.1537 0.1845 0.1709 0.091
Nuclear Periphery 0.0016 0.0034 0.0075 0.0046 0.0008 0.0024 0.014 0.0032 0.0038 0.0043 0.0047 0.0061 0.0097 0.0032 0.0056 0.0037 0.0074 0.0062
Nucleolus 0.0001 0.0005 0.0002 0.0002 0.0002 0.0001 0.0007 0.0004 0.0002 0.0003 0.0084 0.0015 0.0009 0.0004 0.0001 0.0001 0.0007 0.0007
Nucleus 0.0098 0.0512 0.0263 0.0456 0.0285 0.047 0.0343 0.0338 0.0463 0.078 0.0696 0.0739 0.0636 0.0484 0.0597 0.1287 0.0832 0.0977
Peroxisomes 0 0.0002 0.0001 0.0001 0 0.0009 0.0227 0.0004 0.0175 0.0522 0.0451 0.0042 0.0204 0.0002 0.0066 0.0001 0.0003 0.0003
Punctate Nuclear 0.0013 0.0095 0.0027 0.0047 0.0021 0.0045 0.0132 0.0031 0.0048 0.0104 0.0064 0.0218 0.0104 0.0033 0.0035 0.0023 0.0284 0.01
Vacuole 0.0009 0.001 0.0011 0.0009 0.0003 0.0008 0.0044 0.0014 0.0014 0.0009 0.0019 0.0013 0.0019 0.0009 0.0011 0.0005 0.0017 0.001
Vacuole Periphery 0.0001 0.0001 0.0003 0.0002 0 0.0001 0.0017 0.0002 0.0004 0.0002 0.0019 0.0004 0.0006 0.0002 0.0003 0.0001 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.2167 8.372 11.86 15.3745 14.3503 12.7638 10.5812 13.5041 13.4202 16.2951
Translational Efficiency 0.8512 0.7639 0.7986 0.7751 0.7112 0.844 0.9199 0.6686 1.0159 0.7641

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ram1

Ram1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ram1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available