Standard name | |
---|---|
Human Ortholog | |
Description | Clathrin-coated vesicle component, regulator of endocytosis; copurifies with the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; required for efficient clathrin-mediated endocytosis; ortholog of fission yeast Ucp10 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 2 | 2 | 2 | 3 | 4 | 0 | 1 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 4 | 2 | 12 | 5 | 4 | 6 |
Bud | 3 | 3 | 9 | 10 | 18 | 13 | 57 | 32 | 43 | 59 | 60 | 60 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 6 | 5 | 3 | 6 | 14 |
Bud Neck | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 4 | 3 | 1 | 1 | 3 | 0 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 241 | 338 | 454 | 193 | 322 | 287 | 483 | 513 | 361 | 446 | 312 | 413 | 311 | 493 | 627 | 59 | 110 | 67 | 87 | 97 | 82 | 165 | 208 | 186 |
Endoplasmic Reticulum | 6 | 8 | 5 | 2 | 2 | 4 | 3 | 2 | 0 | 0 | 3 | 8 | 3 | 7 | 2 | 5 | 10 | 4 | 8 | 12 | 17 | 8 | 9 | 21 |
Endosome | 1 | 15 | 58 | 30 | 50 | 17 | 37 | 99 | 47 | 18 | 19 | 16 | 12 | 4 | 12 | 14 | 7 | 6 | 55 | 76 | 153 | 28 | 37 | 59 |
Golgi | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 8 | 24 | 3 | 3 | 7 |
Mitochondria | 87 | 27 | 32 | 34 | 70 | 130 | 381 | 234 | 252 | 418 | 370 | 378 | 1 | 0 | 0 | 4 | 2 | 1 | 93 | 78 | 211 | 103 | 112 | 126 |
Nucleus | 0 | 1 | 0 | 1 | 3 | 3 | 4 | 5 | 4 | 4 | 3 | 2 | 0 | 1 | 0 | 1 | 6 | 3 | 0 | 1 | 1 | 4 | 3 | 1 |
Nuclear Periphery | 1 | 0 | 2 | 1 | 1 | 0 | 1 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 6 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 7 | 1 | 2 | 0 | 3 | 8 | 0 | 1 | 1 | 1 | 1 | 0 | 6 | 0 | 0 | 0 | 3 | 0 | 2 | 1 | 0 | 0 |
SpindlePole | 4 | 0 | 14 | 7 | 14 | 14 | 20 | 35 | 15 | 9 | 15 | 19 | 3 | 1 | 2 | 0 | 1 | 0 | 9 | 4 | 7 | 2 | 9 | 15 |
Vac/Vac Membrane | 10 | 50 | 36 | 7 | 31 | 56 | 82 | 53 | 50 | 66 | 54 | 75 | 13 | 7 | 46 | 82 | 74 | 69 | 82 | 178 | 130 | 117 | 177 | 221 |
Unique Cell Count | 307 | 410 | 571 | 265 | 446 | 433 | 859 | 832 | 633 | 798 | 639 | 748 | 336 | 506 | 656 | 133 | 165 | 129 | 355 | 477 | 663 | 451 | 583 | 686 |
Labelled Cell Count | 355 | 446 | 621 | 288 | 516 | 531 | 1082 | 989 | 779 | 1030 | 843 | 980 | 351 | 522 | 698 | 166 | 210 | 150 | 355 | 477 | 663 | 451 | 583 | 686 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 5.1 | 4.6 | 4.6 | 4.8 | 8.0 | 4.3 | 4.3 | 4.4 | 4.2 | 4.0 | 4.1 | 6.0 | 5.7 | 5.4 | 6.8 | 6.7 | 6.9 | 5.9 | 6.2 | 6.4 |
Std Deviation (1e-4) | 0.5 | 0.9 | 0.9 | 1.4 | 1.6 | 9.5 | 1.1 | 1.1 | 1.1 | 0.9 | 1.0 | 0.9 | 1.0 | 0.9 | 0.8 | 1.3 | 1.4 | 1.4 | 1.3 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.08 | 0.81 | -0.11 | -0.08 | -0.06 | -0.13 | -0.19 | -0.18 | 0.38 | 0.33 | 0.23 | 0.58 | 0.56 | 0.6 | 0.35 | 0.43 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.1 | -2.7 | -4.7 | -9.1 | -7.1 | -8.3 | -9.1 | -11.1 | -9.2 | 5.2 | 9.0 | 8.7 | -8.2 | -3.4 | -6.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | -3.7 | 0 | 0 | 0 | 0 | 0 | 0 | -3.6 | -6.6 | -6.3 | 0.1 | -2.4 | -2.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 10.4 | 0 | 0 | 0 | 0 | 0 | 0 | -4.1 | -5.4 | -6.1 | -1.2 | -2.4 | -2.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.5 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | -1.7 | -3.2 | -3.3 | 0 | 0 | 0 |
Vacuole | -2.2 | 0.4 | 3.6 | 2.2 | 0 | 1.1 | 1.4 | 1.4 | 2.4 | -1.6 | -4.1 | 0.5 | 15.4 | 12.2 | 13.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0585 | 1.9796 | 1.7831 | 1.4214 | 1.7918 | 1.8847 | 0.8385 | 1.1162 | 0.9983 | 0.8511 | 0.686 | 1.1715 | -0.1206 | 0.6182 | 0.1066 | 0.431 | 0.1971 | 0.342 |
Actin | 0.024 | 0.0002 | 0.0053 | 0.0005 | 0.015 | 0.008 | 0.0194 | 0.0028 | 0.0035 | 0.0018 | 0.0033 | 0.0053 | 0.0051 | 0.0009 | 0.0012 | 0 | 0.0121 | 0.002 |
Bud | 0.0006 | 0.0002 | 0.0006 | 0.0005 | 0.0008 | 0.0031 | 0.0003 | 0.0028 | 0.001 | 0.0051 | 0.0005 | 0.0017 | 0.0007 | 0.0019 | 0.0033 | 0.0005 | 0.0007 | 0.003 |
Bud Neck | 0.0056 | 0.0001 | 0.0009 | 0.0003 | 0.0005 | 0.0013 | 0.0015 | 0.001 | 0.0045 | 0.0004 | 0.0007 | 0.001 | 0.0022 | 0.0004 | 0.0005 | 0.0003 | 0.0019 | 0.0016 |
Bud Periphery | 0.0009 | 0.0002 | 0.0007 | 0.0006 | 0.0019 | 0.0035 | 0.0003 | 0.0033 | 0.001 | 0.0107 | 0.0004 | 0.004 | 0.0008 | 0.0014 | 0.0023 | 0.0003 | 0.0005 | 0.0043 |
Bud Site | 0.0042 | 0.0007 | 0.0015 | 0.0004 | 0.001 | 0.0003 | 0.0015 | 0.0154 | 0.0101 | 0.0022 | 0.0004 | 0.001 | 0.0048 | 0.0069 | 0.008 | 0.0002 | 0.0008 | 0.0008 |
Cell Periphery | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0017 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0 | 0.0002 | 0.0001 |
Cytoplasm | 0.1675 | 0.2957 | 0.2377 | 0.2875 | 0.2713 | 0.3137 | 0.2457 | 0.3359 | 0.3453 | 0.2669 | 0.3246 | 0.4367 | 0.3191 | 0.4005 | 0.3553 | 0.3774 | 0.2929 | 0.4181 |
Cytoplasmic Foci | 0.0993 | 0.0556 | 0.0737 | 0.077 | 0.0809 | 0.0842 | 0.0937 | 0.0868 | 0.0711 | 0.0593 | 0.0749 | 0.0844 | 0.0941 | 0.0904 | 0.0935 | 0.1162 | 0.0763 | 0.1063 |
Eisosomes | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0014 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0013 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0029 | 0.0124 | 0.0035 | 0.0061 | 0.0094 | 0.0072 | 0.0055 | 0.0063 | 0.0045 | 0.0032 | 0.0051 | 0.0084 | 0.0038 | 0.0043 | 0.002 | 0.0048 | 0.0039 |
Endosome | 0.1704 | 0.1036 | 0.1572 | 0.1902 | 0.3135 | 0.1444 | 0.1674 | 0.1702 | 0.1421 | 0.2956 | 0.3455 | 0.1788 | 0.2177 | 0.1732 | 0.2239 | 0.3329 | 0.2333 | 0.2106 |
Golgi | 0.0157 | 0.0022 | 0.0057 | 0.0054 | 0.0137 | 0.0046 | 0.0078 | 0.0054 | 0.0064 | 0.0047 | 0.0107 | 0.0038 | 0.0057 | 0.006 | 0.0104 | 0.0086 | 0.0087 | 0.0048 |
Lipid Particles | 0.0189 | 0.0024 | 0.0097 | 0.0033 | 0.0202 | 0.0036 | 0.0072 | 0.0023 | 0.0026 | 0.0014 | 0.011 | 0.0026 | 0.0056 | 0.002 | 0.0024 | 0.0017 | 0.0053 | 0.002 |
Mitochondria | 0.0164 | 0.0011 | 0.0081 | 0.0064 | 0.0327 | 0.0028 | 0.0028 | 0.0036 | 0.0037 | 0.0061 | 0.003 | 0.0038 | 0.0045 | 0.0049 | 0.0031 | 0.0029 | 0.0037 | 0.004 |
None | 0.3738 | 0.4997 | 0.4347 | 0.3714 | 0.1649 | 0.3738 | 0.3882 | 0.3197 | 0.359 | 0.1989 | 0.13 | 0.2309 | 0.2833 | 0.2715 | 0.2553 | 0.0947 | 0.2142 | 0.2086 |
Nuclear Periphery | 0.0039 | 0.0014 | 0.0037 | 0.0024 | 0.0111 | 0.0058 | 0.0089 | 0.0037 | 0.004 | 0.0307 | 0.0065 | 0.006 | 0.0057 | 0.0013 | 0.0016 | 0.0009 | 0.0098 | 0.0016 |
Nucleolus | 0.0033 | 0.0003 | 0.0009 | 0.0005 | 0.0016 | 0.0003 | 0.0007 | 0.0005 | 0.0005 | 0.0009 | 0.0005 | 0.0003 | 0.0005 | 0.0005 | 0.0004 | 0.0002 | 0.0046 | 0.0002 |
Nucleus | 0.004 | 0.0012 | 0.0021 | 0.002 | 0.0036 | 0.0031 | 0.0056 | 0.003 | 0.0023 | 0.009 | 0.0031 | 0.0032 | 0.0026 | 0.0021 | 0.0019 | 0.0021 | 0.0736 | 0.0015 |
Peroxisomes | 0.0096 | 0.0005 | 0.0046 | 0.0012 | 0.0026 | 0.0042 | 0.0074 | 0.0019 | 0.0015 | 0.0016 | 0.0032 | 0.0009 | 0.0023 | 0.0009 | 0.0013 | 0.0007 | 0.0021 | 0.0019 |
Punctate Nuclear | 0.0242 | 0.0008 | 0.0023 | 0.0009 | 0.0047 | 0.0051 | 0.0066 | 0.0021 | 0.0018 | 0.0052 | 0.0117 | 0.0017 | 0.0018 | 0.0013 | 0.0015 | 0.0004 | 0.0158 | 0.0012 |
Vacuole | 0.0447 | 0.0271 | 0.0291 | 0.0359 | 0.0376 | 0.023 | 0.0231 | 0.0274 | 0.0266 | 0.0692 | 0.0508 | 0.0228 | 0.028 | 0.0247 | 0.0245 | 0.0486 | 0.0297 | 0.0175 |
Vacuole Periphery | 0.0055 | 0.0038 | 0.0077 | 0.0098 | 0.0146 | 0.0054 | 0.0038 | 0.0048 | 0.0064 | 0.0255 | 0.016 | 0.0058 | 0.0065 | 0.0049 | 0.0053 | 0.0092 | 0.0078 | 0.0058 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.4527 | 4.3262 | 6.4205 | 6.9928 | 6.2746 | 2.4184 | 6.9259 | 8.3649 | 9.3749 | 8.362 |
Translational Efficiency | 0.5075 | 0.8929 | 0.5129 | 0.5587 | 0.5105 | 1.9765 | 0.6487 | 0.5225 | 0.6148 | 0.7096 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2464 | 792 | 2253 | 1610 | 1973 | 2361 | 1249 | 99 | 4437 | 3153 | 3502 | 1709 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 724.36 | 708.15 | 817.49 | 794.84 | 685.60 | 735.68 | 852.45 | 885.19 | 707.12 | 728.76 | 829.96 | 800.07 |
Standard Deviation | 83.42 | 117.74 | 105.66 | 107.47 | 81.48 | 88.17 | 109.43 | 147.05 | 84.78 | 97.19 | 108.32 | 112.15 |
Intensity Change Log 2 | — | -0.032652 | 0.174494 | 0.133958 | — | 0.101711 | 0.314248 | 0.368620 | — | 0.034247 | 0.244142 | 0.252833 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000263 | 0.000678 | 0.000435 | 0.000785 | 0.000257 | 0.000487 | 0.000218 | 0.000178 | 0.000261 | 0.000535 | 0.000358 | 0.000750 |
Bud Neck | 0.016245 | 0.018923 | 0.016840 | 0.013391 | 0.014201 | 0.019195 | 0.010453 | 0.002482 | 0.015336 | 0.019127 | 0.014562 | 0.012759 |
Bud Site | 0.007240 | 0.018675 | 0.022653 | 0.047276 | 0.006366 | 0.018823 | 0.014109 | 0.038216 | 0.006852 | 0.018786 | 0.019606 | 0.046751 |
Cell Periphery | 0.000081 | 0.000126 | 0.000074 | 0.000195 | 0.000079 | 0.000071 | 0.000077 | 0.000135 | 0.000080 | 0.000085 | 0.000075 | 0.000191 |
Cytoplasm | 0.252415* | 0.230476* | 0.441693* | 0.452453* | 0.304653* | 0.225898* | 0.371664* | 0.385808* | 0.275643* | 0.227048* | 0.416717* | 0.448592* |
Cytoplasmic Foci | 0.376202* | 0.365068* | 0.093141 | 0.034409 | 0.404938* | 0.368387* | 0.050262 | 0.039919 | 0.388980* | 0.367553* | 0.077848 | 0.034728 |
Eisosomes | 0.000154 | 0.000122 | 0.000029 | 0.000048 | 0.000169 | 0.000105 | 0.000040 | 0.000057 | 0.000161 | 0.000109 | 0.000033 | 0.000049 |
Endoplasmic Reticulum | 0.003310 | 0.002254 | 0.003219 | 0.002088 | 0.003183 | 0.001389 | 0.003252 | 0.001603 | 0.003254 | 0.001606 | 0.003231 | 0.002060 |
Endosome | 0.072918 | 0.122078 | 0.033056 | 0.011651 | 0.054600 | 0.094360 | 0.034110 | 0.012161 | 0.064773 | 0.101322 | 0.033432 | 0.011681 |
Golgi | 0.021453 | 0.021159 | 0.001946 | 0.004604 | 0.017531 | 0.030143 | 0.000400 | 0.023959 | 0.019709 | 0.027887 | 0.001395 | 0.005725 |
Lipid Particles | 0.024238 | 0.016889 | 0.004829 | 0.001753 | 0.021149 | 0.011763 | 0.003969 | 0.001694 | 0.022865 | 0.013051 | 0.004522 | 0.001750 |
Mitochondria | 0.014306 | 0.011921 | 0.003452 | 0.010833 | 0.011120 | 0.023019 | 0.002107 | 0.006637 | 0.012889 | 0.020231 | 0.002972 | 0.010590 |
Mitotic Spindle | 0.003601 | 0.005417 | 0.032186 | 0.051723 | 0.002117 | 0.017414 | 0.006859 | 0.005475 | 0.002941 | 0.014400 | 0.023153 | 0.049044 |
None | 0.001028 | 0.002755 | 0.001905 | 0.004596 | 0.000692 | 0.000593 | 0.004513 | 0.002040 | 0.000879 | 0.001136 | 0.002835 | 0.004448 |
Nuclear Periphery | 0.000815 | 0.000438 | 0.005244 | 0.002204 | 0.000452 | 0.000375 | 0.003735 | 0.001227 | 0.000654 | 0.000391 | 0.004705 | 0.002148 |
Nuclear Periphery Foci | 0.000400 | 0.001163 | 0.003276 | 0.001251 | 0.000435 | 0.000488 | 0.002930 | 0.002129 | 0.000416 | 0.000657 | 0.003152 | 0.001302 |
Nucleolus | 0.000980 | 0.002533 | 0.002242 | 0.000801 | 0.000629 | 0.000833 | 0.003934 | 0.000613 | 0.000824 | 0.001260 | 0.002845 | 0.000790 |
Nucleus | 0.041786 | 0.024464 | 0.140621* | 0.162427* | 0.045732 | 0.019315 | 0.220195* | 0.094051 | 0.043541 | 0.020609 | 0.169001* | 0.158466* |
Peroxisomes | 0.010547 | 0.020762 | 0.004774 | 0.001420 | 0.008941 | 0.016137 | 0.003865 | 0.000601 | 0.009833 | 0.017299 | 0.004450 | 0.001373 |
Vacuole | 0.145125 | 0.125374 | 0.185451 | 0.191812 | 0.098597 | 0.141526 | 0.260787* | 0.355029* | 0.124435 | 0.137469 | 0.212320* | 0.201267* |
Vacuole Periphery | 0.006892 | 0.008723 | 0.002933 | 0.004280 | 0.004160 | 0.009678 | 0.002522 | 0.025985 | 0.005677 | 0.009438 | 0.002787 | 0.005538 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.82 | -2.97 | -1.89 | 1.17 | -0.07 | -2.75 | 0.56 | -0.03 | 2.53 | -0.45 | -3.62 | -2.84 | -2.04 | 0.59 | -0.78 |
Bud Neck | -1.51 | -1.29 | 5.88 | 5.22 | 6.27 | -4.24 | 2.18 | 9.82 | 13.60 | 5.79 | -4.04 | -0.20 | 6.29 | 8.89 | 5.70 |
Bud Site | -5.62 | -6.38 | -6.90 | -1.90 | -1.73 | -8.95 | -2.83 | -0.90 | 0.42 | -0.32 | -10.29 | -7.05 | -7.20 | -2.00 | -2.73 |
Cell Periphery | -3.60 | 1.55 | -3.25 | 0.62 | -4.17 | 1.85 | 1.07 | -1.84 | -2.45 | -2.20 | -0.98 | 1.92 | -3.61 | -3.00 | -4.37 |
Cytoplasm | 2.08 | -21.61 | -16.53 | -14.28 | 3.21 | 10.16 | -6.09 | 0.07 | -2.71 | 2.10 | 8.29 | -20.47 | -14.49 | -19.93 | 1.69 |
Cytoplasmic Foci | 1.37 | 54.12 | 73.89* | 41.44 | 20.66 | 5.19 | 64.09 | 48.80 | 45.13 | 1.52 | 4.10 | 80.15 | 99.68 | 78.63 | 19.88 |
Eisosomes | 3.50 | 18.64 | 16.00 | 11.22 | -7.87 | 8.39 | 18.37 | 10.07 | 3.95 | -5.24 | 9.12 | 26.33 | 22.62 | 16.85 | -7.45 |
Endoplasmic Reticulum | 2.22 | -0.88 | 3.81 | -0.08 | 6.20 | 4.37 | -0.54 | 1.55 | -3.36 | 3.01 | 6.37 | -1.04 | 3.91 | -4.32 | 7.54 |
Endosome | -8.56 | 17.95 | 32.50* | 21.78 | 14.11 | -12.75 | 9.42 | 19.70 | 31.93 | 8.13 | -13.71 | 19.65 | 38.83* | 41.72 | 16.53 |
Golgi | 0.30 | 17.26 | 16.03 | 10.00 | -0.37 | -7.21 | 15.85 | 1.84 | 3.89 | -1.05 | -5.89 | 22.93 | 17.75 | 20.16 | -1.42 |
Lipid Particles | 3.90 | 19.65 | 23.47 | 9.57 | 12.82 | 8.01 | 17.06 | 18.67 | 15.58 | 5.25 | 10.64 | 25.78 | 30.28 | 18.87 | 14.49 |
Mitochondria | 1.64 | 12.63 | 7.24 | 4.37 | -1.39 | -7.19 | 8.43 | 5.00 | 10.32 | -0.12 | -5.91 | 14.91 | 7.21 | 10.99 | -1.81 |
Mitotic Spindle | -1.50 | -11.79 | -8.00 | -7.06 | 1.19 | -8.41 | -5.18 | -1.41 | 0.91 | -0.39 | -8.24 | -12.78 | -8.37 | -4.08 | -0.91 |
None | -1.72 | -4.24 | -4.38 | -0.62 | -2.94 | 1.12 | -6.41 | -3.02 | -3.13 | 0.80 | -0.97 | -8.01 | -5.05 | -4.12 | -1.42 |
Nuclear Periphery | 2.81 | -11.29 | -11.69 | -15.06 | 5.15 | 1.05 | -11.67 | -5.61 | -5.82 | 4.63 | 3.29 | -15.20 | -14.14 | -16.27 | 5.70 |
Nuclear Periphery Foci | -4.69 | -8.42 | -8.07 | 0.15 | 6.17 | -0.26 | -6.73 | -4.47 | -4.00 | 1.30 | -1.58 | -10.72 | -9.15 | -3.26 | 7.42 |
Nucleolus | -3.42 | -7.53 | 2.72 | 3.98 | 8.76 | -2.98 | -7.42 | 0.17 | 0.89 | 6.32 | -3.44 | -10.74 | 1.53 | 4.07 | 11.03 |
Nucleus | 5.44 | -26.73 | -30.16 | -30.92 | -8.25 | 13.67 | -27.83 | -5.44 | -6.97 | 5.07 | 15.05 | -37.87 | -30.76 | -35.25 | -3.53 |
Peroxisomes | -4.78 | 7.86 | 16.28 | 9.65 | 7.92 | -6.84 | 6.55 | 16.18 | 17.22 | 3.48 | -7.89 | 10.18 | 21.51 | 19.04 | 7.81 |
Vacuole | 2.75 | -10.55 | -18.44 | -17.89 | -9.77 | -9.43 | -21.53 | -11.18 | -9.81 | -5.73 | -3.64 | -22.52 | -23.98 | -21.23 | -8.54 |
Vacuole Periphery | -2.17 | 11.51 | 10.06 | 8.57 | 1.45 | -10.68 | 8.01 | -0.45 | 0.40 | -0.80 | -8.30 | 13.58 | 6.52 | 11.39 | 0.30 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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