Standard name | |
---|---|
Human Ortholog | |
Description | Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 2 | 3 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 7 | 2 | 5 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 5 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 6 | 10 | 7 | 8 | 15 | 15 | 26 | 18 | 17 | 8 | 10 | 23 | 36 | 71 | 31 | 29 | 22 | 0 | 0 | 0 | 4 | 3 | 5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 6 | 11 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 5 | 5 | 1 | 1 | 0 | 1 | 2 | 4 | 9 | 6 | 4 | 0 | 0 | 2 | 0 | 0 | 3 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 7 | 16 | 9 | 9 | 37 | 22 | 33 | 1 | 0 | 0 | 2 | 6 | 2 | 0 | 1 | 0 | 1 | 1 | 4 |
Nucleus | 33 | 257 | 233 | 150 | 100 | 89 | 134 | 121 | 101 | 118 | 87 | 78 | 219 | 181 | 208 | 132 | 132 | 91 | 56 | 264 | 209 | 261 | 223 | 237 |
Nuclear Periphery | 0 | 0 | 4 | 4 | 4 | 3 | 9 | 5 | 2 | 6 | 4 | 7 | 1 | 3 | 2 | 2 | 2 | 0 | 0 | 0 | 4 | 0 | 2 | 5 |
Nucleolus | 1 | 18 | 5 | 3 | 5 | 9 | 9 | 12 | 12 | 13 | 27 | 16 | 3 | 1 | 3 | 6 | 10 | 5 | 5 | 33 | 81 | 15 | 24 | 63 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Vac/Vac Membrane | 4 | 34 | 27 | 22 | 103 | 79 | 106 | 105 | 71 | 70 | 60 | 62 | 23 | 52 | 76 | 69 | 82 | 64 | 3 | 15 | 26 | 25 | 44 | 118 |
Unique Cell Count | 36 | 301 | 274 | 181 | 211 | 196 | 278 | 274 | 213 | 255 | 201 | 197 | 269 | 261 | 356 | 241 | 255 | 184 | 68 | 325 | 339 | 318 | 315 | 462 |
Labelled Cell Count | 38 | 315 | 279 | 186 | 221 | 208 | 294 | 290 | 224 | 273 | 216 | 214 | 274 | 278 | 373 | 258 | 279 | 195 | 68 | 325 | 339 | 318 | 315 | 462 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.3 | 29.0 | 23.2 | 21.1 | 21.2 | 20.0 | 19.5 | 18.9 | 18.5 | 17.4 | 16.4 | 14.6 | 24.6 | 25.4 | 22.1 | 27.5 | 23.0 | 26.6 | 24.9 | 23.8 | 26.0 |
Std Deviation (1e-4) | 3.4 | 6.0 | 5.8 | 4.5 | 4.5 | 4.2 | 4.2 | 5.1 | 5.1 | 5.2 | 4.7 | 4.9 | 6.9 | 7.8 | 6.7 | 8.8 | 7.6 | 8.7 | 6.2 | 6.6 | 7.7 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.13 | -0.22 | -0.25 | -0.3 | -0.32 | -0.42 | -0.5 | -0.66 | 0.08 | 0.13 | -0.07 | 0.25 | -0.01 | 0.2 | 0.1 | 0.04 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | 0.1 | 1.9 | 1.0 | 2.8 | 2.3 | 1.6 | 0.2 | 0.8 | 2.4 | 4.2 | 6.1 | 3.9 | 3.4 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.0 | 0 | 0 | 6.5 | 5.6 | 7.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.6 | -8.9 | -9.1 | -9.2 | -10.0 | -8.9 | -9.4 | -9.6 | -10.3 | -1.1 | -4.3 | -7.2 | -7.5 | -8.3 | -8.2 |
Nuclear Periphery | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.7 | 1.1 | 1.7 | 2.3 | 2.1 | 5.0 | 3.3 | 0 | 0 | 0 | 0.5 | 1.5 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.8 | 9.6 | 7.8 | 7.8 | 7.8 | 6.4 | 5.2 | 5.6 | 5.9 | -0.5 | 3.3 | 3.9 | 5.5 | 6.3 | 6.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 24.2761 | 26.1751 | 24.4194 | 23.6228 | 24.1004 | 24.0111 | 17.7138 | 29.5056 | 25.6913 | 23.1221 | 21.4815 | 23.4922 | 23.816 | 31.4337 | 28.933 | 27.6683 | 27.8896 | 28.6116 |
Actin | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0348 | 0.0001 | 0.0146 | 0.0018 | 0.0001 | 0.0033 | 0.0065 | 0.0016 | 0.0027 | 0.0005 | 0.0001 | 0.0046 |
Bud | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0012 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0011 | 0.0005 | 0.002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0021 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0006 |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0.0017 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.0026 | 0.0007 | 0.001 | 0.0005 | 0.0001 | 0.0001 | 0.0003 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0027 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0.0012 | 0.0014 | 0.0011 | 0.0001 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0012 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 |
Cytoplasm | 0.0007 | 0.001 | 0.0004 | 0.0002 | 0.0003 | 0.0008 | 0.0369 | 0.0029 | 0.0018 | 0.0027 | 0.0011 | 0.0086 | 0.0219 | 0.0026 | 0.0071 | 0.0051 | 0.0003 | 0.0018 |
Cytoplasmic Foci | 0 | 0.0008 | 0.0007 | 0 | 0 | 0 | 0.0217 | 0 | 0.0061 | 0.0005 | 0 | 0.0021 | 0.0185 | 0.0012 | 0.004 | 0.0072 | 0 | 0.0003 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0101 | 0 | 0.0002 | 0.0008 | 0.0001 | 0.0041 | 0.0032 | 0.0003 | 0.0005 | 0.0007 | 0 | 0.0004 |
Endosome | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0261 | 0 | 0.0006 | 0.0018 | 0.0001 | 0.031 | 0.0243 | 0.0014 | 0.0036 | 0.0006 | 0 | 0.0015 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0.0063 | 0 | 0.0005 | 0.0001 | 0 | 0.0037 | 0.0026 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0009 |
Lipid Particles | 0 | 0.0002 | 0.0007 | 0 | 0 | 0.0001 | 0.0092 | 0 | 0.0016 | 0.0001 | 0 | 0.0024 | 0.0083 | 0.0002 | 0.0006 | 0.0026 | 0 | 0.0005 |
Mitochondria | 0.0009 | 0.0005 | 0.0005 | 0.0004 | 0.0012 | 0.0004 | 0.0121 | 0.0007 | 0.0028 | 0.0015 | 0.0007 | 0.0043 | 0.0046 | 0.0007 | 0.0052 | 0.0016 | 0.0006 | 0.0061 |
None | 0 | 0.0001 | 0.0101 | 0 | 0 | 0 | 0.0114 | 0.0001 | 0.0012 | 0.0005 | 0.0001 | 0.0005 | 0.0039 | 0.0002 | 0.0005 | 0.0041 | 0 | 0.0002 |
Nuclear Periphery | 0.0148 | 0.0157 | 0.0079 | 0.0041 | 0.0071 | 0.0062 | 0.0749 | 0.0065 | 0.004 | 0.0377 | 0.0035 | 0.0137 | 0.0407 | 0.0202 | 0.0101 | 0.018 | 0.0148 | 0.0116 |
Nucleolus | 0.0073 | 0.0147 | 0.0099 | 0.0061 | 0.0407 | 0.0172 | 0.0158 | 0.004 | 0.0172 | 0.0024 | 0.0352 | 0.0334 | 0.0545 | 0.0078 | 0.0077 | 0.0379 | 0.0232 | 0.0176 |
Nucleus | 0.9732 | 0.9656 | 0.9619 | 0.9887 | 0.9484 | 0.9697 | 0.6492 | 0.9848 | 0.9358 | 0.9392 | 0.9543 | 0.8651 | 0.7598 | 0.9561 | 0.9482 | 0.9047 | 0.9595 | 0.9481 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0075 | 0 | 0.0031 | 0.0001 | 0 | 0.0002 | 0.0024 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0002 | 0.0006 | 0.0014 | 0.0001 | 0.0006 | 0.0002 | 0.0632 | 0.0001 | 0.0047 | 0.0097 | 0.0005 | 0.0051 | 0.029 | 0.0021 | 0.0032 | 0.0132 | 0.0003 | 0.0008 |
Vacuole | 0.0009 | 0.0002 | 0.0046 | 0.0001 | 0.0003 | 0.0026 | 0.0072 | 0.0002 | 0.0021 | 0.0003 | 0.0018 | 0.0081 | 0.0077 | 0.0003 | 0.0015 | 0.0007 | 0.0003 | 0.0016 |
Vacuole Periphery | 0.0015 | 0.0003 | 0.0012 | 0.0001 | 0.0006 | 0.0009 | 0.0065 | 0.0002 | 0.0022 | 0.0005 | 0.0012 | 0.0069 | 0.0086 | 0.0005 | 0.0015 | 0.002 | 0.0005 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 99.3487 | 92.2129 | 88.0564 | 116.6405 | 89.3447 | 109.8568 | 100.3056 | 118.6353 | 118.603 | 107.4355 |
Translational Efficiency | 2.4868 | 1.9371 | 2.3097 | 1.8437 | 2.199 | 2.414 | 1.9612 | 1.7047 | 1.8751 | 1.7421 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1913 | 769 | 2298 | 1522 | 1932 | 934 | 1621 | 1488 | 3845 | 1703 | 3919 | 3010 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1686.92 | 2025.22 | 1969.79 | 1990.22 | 1629.13 | 1813.98 | 1989.98 | 1851.32 | 1657.88 | 1909.37 | 1978.14 | 1921.55 |
Standard Deviation | 295.35 | 369.47 | 358.04 | 398.49 | 298.08 | 319.71 | 424.84 | 430.78 | 298.13 | 358.82 | 387.20 | 420.54 |
Intensity Change Log 2 | — | 0.263687 | 0.223650 | 0.238536 | — | 0.155057 | 0.288652 | 0.184453 | — | 0.211340 | 0.255951 | 0.212219 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000912 | 0.001082 | 0.000841 | 0.001523 | 0.000911 | 0.000892 | 0.001021 | 0.001280 | 0.000911 | 0.000978 | 0.000916 | 0.001403 |
Bud Neck | 0.012718 | 0.009429 | 0.025145 | 0.040919 | 0.012533 | 0.011575 | 0.023170 | 0.037212 | 0.012625 | 0.010606 | 0.024328 | 0.039086 |
Bud Site | 0.000469 | 0.000496 | 0.000692 | 0.005485 | 0.000475 | 0.000643 | 0.001943 | 0.006129 | 0.000472 | 0.000576 | 0.001210 | 0.005804 |
Cell Periphery | 0.000056 | 0.000043 | 0.000052 | 0.000274 | 0.000082 | 0.000085 | 0.000115 | 0.000221 | 0.000069 | 0.000066 | 0.000078 | 0.000248 |
Cytoplasm | 0.000642 | 0.001316 | 0.000526 | 0.004085 | 0.000591 | 0.000414 | 0.002063 | 0.018137 | 0.000616 | 0.000821 | 0.001162 | 0.011032 |
Cytoplasmic Foci | 0.000042 | 0.000650 | 0.000044 | 0.001524 | 0.000060 | 0.000417 | 0.000338 | 0.002171 | 0.000051 | 0.000522 | 0.000166 | 0.001844 |
Eisosomes | 0.000024 | 0.000030 | 0.000024 | 0.000021 | 0.000026 | 0.000029 | 0.000030 | 0.000013 | 0.000025 | 0.000029 | 0.000027 | 0.000017 |
Endoplasmic Reticulum | 0.002980 | 0.001457 | 0.001451 | 0.002279 | 0.003708 | 0.001859 | 0.001132 | 0.002266 | 0.003346 | 0.001677 | 0.001319 | 0.002273 |
Endosome | 0.000112 | 0.000363 | 0.000393 | 0.001790 | 0.000152 | 0.000183 | 0.000741 | 0.003019 | 0.000132 | 0.000264 | 0.000537 | 0.002397 |
Golgi | 0.000084 | 0.000428 | 0.000096 | 0.002448 | 0.000093 | 0.000578 | 0.000444 | 0.002382 | 0.000089 | 0.000510 | 0.000240 | 0.002415 |
Lipid Particles | 0.000023 | 0.000594 | 0.000050 | 0.000202 | 0.000028 | 0.000130 | 0.000084 | 0.000479 | 0.000025 | 0.000340 | 0.000064 | 0.000339 |
Mitochondria | 0.002193 | 0.002437 | 0.003289 | 0.009242 | 0.002325 | 0.002293 | 0.004002 | 0.010991 | 0.002259 | 0.002358 | 0.003584 | 0.010107 |
Mitotic Spindle | 0.000240 | 0.003014 | 0.000854 | 0.028809 | 0.000756 | 0.001784 | 0.004900 | 0.047518 | 0.000500 | 0.002340 | 0.002528 | 0.038058 |
None | 0.000740 | 0.001061 | 0.001167 | 0.002186 | 0.000745 | 0.002073 | 0.002491 | 0.004753 | 0.000742 | 0.001616 | 0.001714 | 0.003455 |
Nuclear Periphery | 0.002410 | 0.000893 | 0.002967 | 0.015416 | 0.004315 | 0.001423 | 0.002695 | 0.045822 | 0.003367 | 0.001183 | 0.002854 | 0.030447 |
Nuclear Periphery Foci | 0.000615 | 0.001023 | 0.000250 | 0.002128 | 0.000660 | 0.000254 | 0.000335 | 0.010333 | 0.000638 | 0.000602 | 0.000285 | 0.006185 |
Nucleolus | 0.061262 | 0.075310 | 0.033868 | 0.042245 | 0.077285 | 0.055121 | 0.041888 | 0.028323 | 0.069313 | 0.064237 | 0.037185 | 0.035363 |
Nucleus | 0.912723* | 0.897948* | 0.927006* | 0.832144* | 0.892837* | 0.918346* | 0.909752* | 0.770744* | 0.902731* | 0.909135* | 0.919869* | 0.801791* |
Peroxisomes | 0.000386 | 0.001072 | 0.000532 | 0.000480 | 0.000426 | 0.000495 | 0.000929 | 0.000671 | 0.000406 | 0.000756 | 0.000696 | 0.000574 |
Vacuole | 0.000622 | 0.000659 | 0.000372 | 0.004470 | 0.000850 | 0.000711 | 0.001302 | 0.004945 | 0.000737 | 0.000687 | 0.000756 | 0.004704 |
Vacuole Periphery | 0.000746 | 0.000696 | 0.000382 | 0.002329 | 0.001140 | 0.000696 | 0.000622 | 0.002591 | 0.000944 | 0.000696 | 0.000481 | 0.002459 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.35 | -0.05 | -4.43 | -2.17 | -4.58 | -0.03 | -4.52 | -7.74 | -5.66 | -4.55 | -1.13 | -2.67 | -7.53 | -4.77 | -6.47 |
Bud Neck | 4.33 | -14.85 | -17.35 | -19.11 | -8.75 | 0.87 | -12.18 | -19.03 | -18.79 | -10.37 | 3.03 | -19.20 | -25.72 | -26.53 | -13.58 |
Bud Site | -0.14 | -2.47 | -6.03 | -6.05 | -5.62 | -1.33 | -5.19 | -5.72 | -5.25 | -3.52 | -1.18 | -5.87 | -8.27 | -7.85 | -6.50 |
Cell Periphery | 1.42 | -0.03 | -3.94 | -4.29 | -3.99 | -0.31 | -1.79 | -5.41 | -4.20 | -4.73 | -0.04 | -1.24 | -6.11 | -5.68 | -5.91 |
Cytoplasm | -0.64 | -0.22 | -5.69 | -3.21 | -5.77 | 0.69 | -4.88 | -10.83 | -11.01 | -9.74 | -0.43 | -3.31 | -12.14 | -10.73 | -11.38 |
Cytoplasmic Foci | -1.66 | -0.16 | -3.20 | -0.80 | -3.19 | -1.06 | -2.36 | -4.09 | -2.43 | -3.50 | -1.90 | -2.25 | -5.19 | -2.36 | -4.81 |
Eisosomes | -3.77 | -2.40 | 1.45 | 4.47 | 3.36 | -1.36 | -4.36 | 8.00 | 9.36 | 13.92 | -3.59 | -4.32 | 6.69 | 9.27 | 11.09 |
Endoplasmic Reticulum | 8.77 | 11.69 | 4.62 | -3.89 | -5.13 | 8.09 | 14.91 | 6.31 | -2.93 | -10.02 | 11.46 | 18.66 | 7.79 | -4.55 | -10.16 |
Endosome | -1.14 | -0.81 | -4.88 | -2.75 | -2.54 | -0.93 | -1.30 | -4.63 | -4.40 | -3.09 | -1.42 | -1.40 | -6.43 | -5.28 | -4.03 |
Golgi | -1.49 | -0.59 | -2.57 | -1.81 | -2.53 | -0.98 | -1.72 | -3.15 | -1.49 | -2.48 | -1.46 | -1.81 | -3.98 | -2.25 | -3.61 |
Lipid Particles | -1.46 | -0.62 | -5.36 | 0.99 | -3.42 | -1.17 | -1.28 | -5.28 | -2.98 | -4.64 | -1.73 | -1.21 | -6.74 | -0.17 | -5.66 |
Mitochondria | -1.46 | -10.72 | -8.18 | -7.71 | -6.28 | 0.03 | -6.33 | -8.06 | -7.92 | -6.00 | -0.80 | -11.01 | -11.41 | -11.00 | -8.71 |
Mitotic Spindle | -1.93 | -2.56 | -8.08 | -6.50 | -7.65 | -1.50 | -3.55 | -11.40 | -10.38 | -9.61 | -2.40 | -4.07 | -13.91 | -12.15 | -12.48 |
None | -0.92 | -2.42 | -7.56 | -4.04 | -4.53 | -1.26 | -7.81 | -8.89 | -2.39 | -4.02 | -1.46 | -6.77 | -11.51 | -3.36 | -6.26 |
Nuclear Periphery | 4.61 | -0.67 | -8.43 | -9.68 | -7.95 | 4.74 | 2.56 | -12.71 | -13.80 | -13.35 | 6.21 | 1.57 | -14.93 | -16.42 | -15.31 |
Nuclear Periphery Foci | -0.80 | 1.46 | -3.52 | -1.73 | -5.61 | 1.14 | 2.04 | -9.51 | -9.82 | -10.05 | -0.17 | 2.39 | -10.04 | -9.46 | -11.22 |
Nucleolus | -2.69 | 7.15 | 5.01 | 6.18 | -1.78 | 4.38 | 9.25 | 11.27 | 4.33 | 1.32 | 1.24 | 11.92 | 11.47 | 7.29 | -0.43 |
Nucleus | 2.34 | -1.33 | 12.08 | 7.86 | 13.26 | -3.80 | -2.16 | 17.16 | 18.33 | 18.19 | -1.07 | -2.98 | 20.67 | 18.79 | 22.69 |
Peroxisomes | -5.35 | -1.30 | -1.87 | 4.72 | 0.85 | -1.67 | -2.75 | -1.05 | -0.15 | 1.25 | -5.31 | -2.53 | -1.52 | 2.67 | 1.33 |
Vacuole | -0.06 | 0.07 | -5.80 | -5.78 | -5.61 | 0.20 | -1.21 | -7.06 | -7.02 | -6.08 | 0.07 | -0.68 | -9.08 | -9.00 | -8.40 |
Vacuole Periphery | 0.37 | 3.90 | -2.80 | -2.92 | -4.00 | 1.94 | 2.86 | -1.88 | -3.16 | -3.73 | 1.82 | 4.45 | -3.26 | -4.28 | -5.56 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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