Standard name | |
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Human Ortholog | |
Description | Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 2 | 0 | 3 | 4 | 5 | 0 | 4 | 8 |
Bud | 2 | 3 | 6 | 7 | 8 | 15 | 29 | 19 | 11 | 11 | 13 | 18 | 2 | 1 | 0 | 10 | 15 | 1 | 1 | 1 | 0 | 0 | 0 | 2 |
Bud Neck | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 2 | 4 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 2 | 10 | 6 | 3 | 5 | 6 | 8 | 0 | 0 | 0 | 1 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 9 | 2 | 2 | 1 | 0 | 0 | 0 | 4 | 1 | 0 | 3 | 2 | 10 | 10 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 40 | 41 | 35 | 52 | 37 | 45 | 45 | 39 | 33 | 28 | 19 | 23 | 83 | 130 | 149 | 36 | 34 | 29 | 10 | 12 | 3 | 7 | 18 | 25 |
Endoplasmic Reticulum | 2 | 2 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 9 | 5 | 6 | 4 | 1 | 2 | 0 | 1 | 1 |
Endosome | 0 | 2 | 15 | 6 | 14 | 0 | 0 | 6 | 5 | 0 | 0 | 0 | 7 | 6 | 9 | 37 | 32 | 16 | 9 | 10 | 39 | 3 | 8 | 6 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 2 | 1 | 0 | 5 | 0 | 0 | 1 |
Mitochondria | 95 | 49 | 102 | 99 | 125 | 215 | 302 | 238 | 171 | 227 | 192 | 231 | 4 | 0 | 1 | 38 | 27 | 16 | 73 | 95 | 181 | 37 | 76 | 108 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 7 | 3 | 5 | 1 | 0 | 3 | 1 | 1 | 0 | 7 | 5 | 4 | 0 | 0 | 0 | 1 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 1 | 0 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 31 | 18 | 17 | 4 | 7 | 5 | 4 | 1 | 1 | 1 | 6 | 0 | 2 | 14 | 16 | 25 | 2 | 1 | 21 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 32 | 10 | 17 | 15 | 21 | 19 | 11 | 11 | 7 | 13 | 1 | 4 | 3 | 29 | 31 | 27 | 2 | 0 | 4 | 1 | 5 | 7 |
Vac/Vac Membrane | 2 | 4 | 11 | 2 | 8 | 10 | 12 | 8 | 12 | 5 | 5 | 6 | 3 | 4 | 5 | 29 | 23 | 24 | 5 | 9 | 3 | 1 | 11 | 13 |
Unique Cell Count | 119 | 101 | 198 | 162 | 192 | 251 | 346 | 276 | 204 | 246 | 208 | 247 | 104 | 147 | 167 | 181 | 161 | 129 | 121 | 141 | 278 | 58 | 141 | 191 |
Labelled Cell Count | 142 | 108 | 243 | 207 | 234 | 309 | 436 | 345 | 256 | 296 | 253 | 308 | 111 | 155 | 175 | 227 | 206 | 161 | 121 | 141 | 278 | 58 | 141 | 191 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.2 | 4.2 | 4.7 | 4.1 | 3.5 | 3.3 | 3.4 | 4.0 | 3.1 | 3.3 | 3.0 | 5.8 | 5.6 | 5.5 | 6.0 | 5.8 | 5.8 | 5.3 | 6.1 | 6.2 |
Std Deviation (1e-4) | 0.8 | 0.5 | 1.3 | 3.8 | 1.8 | 1.6 | 1.5 | 1.7 | 3.2 | 1.4 | 2.6 | 1.3 | 1.9 | 1.4 | 1.6 | 1.5 | 1.5 | 1.6 | 0.9 | 2.0 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.17 | -0.03 | -0.27 | -0.34 | -0.3 | -0.06 | -0.44 | -0.33 | -0.48 | 0.47 | 0.4 | 0.39 | 0.52 | 0.46 | 0.47 | 0.32 | 0.54 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.3 | 2.8 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.9873 | -0.46 | -0.7001 | -0.7159 | -0.9452 | -0.6307 | -0.512 | -0.2217 | -0.0968 | 0.0265 | -0.2213 | -0.2344 | -0.274 | -0.2429 | -0.2687 | -0.4039 | -0.3866 | -0.5893 |
Actin | 0.0383 | 0.0178 | 0.0087 | 0.0029 | 0.0145 | 0.0122 | 0.0272 | 0.0181 | 0.012 | 0.0164 | 0.0273 | 0.015 | 0.0679 | 0.0155 | 0.0431 | 0.0257 | 0.1077 | 0.0171 |
Bud | 0.0022 | 0.001 | 0.0017 | 0.0014 | 0.0073 | 0.0027 | 0.0041 | 0.0043 | 0.0032 | 0.007 | 0.0049 | 0.0026 | 0.0017 | 0.0052 | 0.0031 | 0.0037 | 0.0018 | 0.0019 |
Bud Neck | 0.0019 | 0.001 | 0.0016 | 0.0014 | 0.0111 | 0.013 | 0.0038 | 0.0033 | 0.0109 | 0.0056 | 0.007 | 0.0056 | 0.0015 | 0.002 | 0.0027 | 0.0033 | 0.0012 | 0.0025 |
Bud Periphery | 0.0028 | 0.0016 | 0.0038 | 0.0052 | 0.0102 | 0.0043 | 0.005 | 0.0056 | 0.01 | 0.0255 | 0.0044 | 0.0029 | 0.0038 | 0.0148 | 0.011 | 0.0136 | 0.0044 | 0.0066 |
Bud Site | 0.006 | 0.0122 | 0.0108 | 0.0022 | 0.0205 | 0.0022 | 0.0161 | 0.0466 | 0.0152 | 0.0188 | 0.0168 | 0.0014 | 0.0048 | 0.0365 | 0.0079 | 0.0043 | 0.0017 | 0.0012 |
Cell Periphery | 0.0013 | 0.0013 | 0.001 | 0.0017 | 0.0008 | 0.0009 | 0.0023 | 0.0019 | 0.0024 | 0.0027 | 0.0003 | 0.0008 | 0.0016 | 0.0026 | 0.0028 | 0.0028 | 0.0005 | 0.001 |
Cytoplasm | 0.0143 | 0.0378 | 0.0207 | 0.0297 | 0.0461 | 0.0247 | 0.0557 | 0.0621 | 0.0622 | 0.0611 | 0.0423 | 0.0699 | 0.0354 | 0.0737 | 0.0796 | 0.0834 | 0.0349 | 0.0509 |
Cytoplasmic Foci | 0.1047 | 0.1243 | 0.1073 | 0.1395 | 0.1174 | 0.1151 | 0.1142 | 0.1205 | 0.1115 | 0.1004 | 0.1455 | 0.1379 | 0.1392 | 0.1428 | 0.0882 | 0.1296 | 0.1459 | 0.0805 |
Eisosomes | 0.0016 | 0.0008 | 0.0016 | 0.0006 | 0.0007 | 0.0005 | 0.0009 | 0.001 | 0.0007 | 0.0009 | 0.0002 | 0.0004 | 0.0023 | 0.0014 | 0.0011 | 0.0007 | 0.0008 | 0.0005 |
Endoplasmic Reticulum | 0.002 | 0.003 | 0.0014 | 0.0023 | 0.0013 | 0.0009 | 0.0114 | 0.0036 | 0.003 | 0.0056 | 0.0063 | 0.0056 | 0.0101 | 0.006 | 0.0133 | 0.003 | 0.0029 | 0.0054 |
Endosome | 0.1131 | 0.0922 | 0.0908 | 0.0783 | 0.0879 | 0.0753 | 0.1426 | 0.1228 | 0.1445 | 0.1284 | 0.134 | 0.1423 | 0.1705 | 0.1442 | 0.1123 | 0.1093 | 0.1454 | 0.153 |
Golgi | 0.0521 | 0.0395 | 0.0463 | 0.0389 | 0.0205 | 0.0479 | 0.0624 | 0.0529 | 0.0655 | 0.0316 | 0.0593 | 0.065 | 0.0691 | 0.0782 | 0.0566 | 0.0298 | 0.0582 | 0.0642 |
Lipid Particles | 0.0581 | 0.0419 | 0.0545 | 0.0248 | 0.0696 | 0.093 | 0.043 | 0.031 | 0.0218 | 0.0542 | 0.0184 | 0.0247 | 0.0568 | 0.0388 | 0.0153 | 0.0157 | 0.0585 | 0.0313 |
Mitochondria | 0.3671 | 0.4085 | 0.4057 | 0.4488 | 0.4005 | 0.3694 | 0.2483 | 0.2843 | 0.3636 | 0.379 | 0.3224 | 0.3227 | 0.3191 | 0.2521 | 0.3597 | 0.3539 | 0.1388 | 0.483 |
None | 0.1161 | 0.1254 | 0.1178 | 0.1379 | 0.0841 | 0.0813 | 0.1921 | 0.1708 | 0.0947 | 0.0516 | 0.1316 | 0.1144 | 0.0648 | 0.1473 | 0.1525 | 0.1178 | 0.1073 | 0.0468 |
Nuclear Periphery | 0.0019 | 0.0014 | 0.0023 | 0.0042 | 0.0021 | 0.0018 | 0.0058 | 0.0012 | 0.0015 | 0.0019 | 0.002 | 0.0033 | 0.0046 | 0.001 | 0.0072 | 0.0013 | 0.0005 | 0.0014 |
Nucleolus | 0.0037 | 0.0013 | 0.003 | 0.0025 | 0.0075 | 0.002 | 0.0015 | 0.0043 | 0.0078 | 0.0049 | 0.0007 | 0.0005 | 0.0007 | 0.0013 | 0.0007 | 0.0003 | 0.0004 | 0.0004 |
Nucleus | 0.0037 | 0.0016 | 0.0023 | 0.0045 | 0.0123 | 0.004 | 0.0025 | 0.0038 | 0.0042 | 0.0019 | 0.0012 | 0.0019 | 0.001 | 0.0013 | 0.0028 | 0.001 | 0.0005 | 0.0007 |
Peroxisomes | 0.0855 | 0.0754 | 0.1036 | 0.0306 | 0.0531 | 0.1365 | 0.0379 | 0.0352 | 0.0465 | 0.0686 | 0.0547 | 0.0687 | 0.0264 | 0.0158 | 0.0248 | 0.0858 | 0.1585 | 0.0316 |
Punctate Nuclear | 0.0133 | 0.0013 | 0.0044 | 0.0335 | 0.0255 | 0.0047 | 0.003 | 0.0061 | 0.0022 | 0.0016 | 0.0044 | 0.0022 | 0.0031 | 0.0018 | 0.0021 | 0.0013 | 0.0017 | 0.0004 |
Vacuole | 0.0078 | 0.0081 | 0.0076 | 0.0063 | 0.0049 | 0.0049 | 0.0158 | 0.0156 | 0.0118 | 0.0242 | 0.0118 | 0.0086 | 0.0103 | 0.0141 | 0.009 | 0.0101 | 0.0179 | 0.0117 |
Vacuole Periphery | 0.0026 | 0.0027 | 0.0031 | 0.0029 | 0.0021 | 0.0029 | 0.0043 | 0.0048 | 0.0046 | 0.008 | 0.0046 | 0.0037 | 0.0051 | 0.0037 | 0.0044 | 0.0037 | 0.0106 | 0.0078 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
991 | 281 | 528 | 1388 | 1359 | 1796 | 364 | 498 | 2350 | 2077 | 892 | 1886 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 626.34 | 822.60 | 825.46 | 753.62 | 602.97 | 696.05 | 891.47 | 773.28 | 612.83 | 713.17 | 852.40 | 758.81 |
Standard Deviation | 72.34 | 245.16 | 90.17 | 111.93 | 83.40 | 102.83 | 105.12 | 96.48 | 79.76 | 138.38 | 101.85 | 108.41 |
Intensity Change Log 2 | — | 0.393245 | 0.398252 | 0.266891 | — | 0.207105 | 0.564100 | 0.358905 | — | 0.304941 | 0.481982 | 0.312757 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000825 | 0.004937 | 0.000997 | 0.009212 | 0.000466 | 0.001495 | 0.002403 | 0.003135 | 0.000617 | 0.001961 | 0.001571 | 0.007607 |
Bud Neck | 0.040056 | 0.035326 | 0.012321 | 0.030042 | 0.037556 | 0.035491 | 0.013238 | 0.026239 | 0.038611 | 0.035469 | 0.012695 | 0.029038 |
Bud Site | 0.019745 | 0.050744 | 0.005704 | 0.072030 | 0.023639 | 0.024400 | 0.016955 | 0.069851 | 0.021997 | 0.027964 | 0.010295 | 0.071454 |
Cell Periphery | 0.000352 | 0.000301 | 0.000295 | 0.000351 | 0.000245 | 0.000227 | 0.000232 | 0.000136 | 0.000290 | 0.000237 | 0.000269 | 0.000294 |
Cytoplasm | 0.054882 | 0.037235 | 0.195892* | 0.323960* | 0.133985* | 0.053739 | 0.258192* | 0.378949* | 0.100627* | 0.051506 | 0.221315* | 0.338480* |
Cytoplasmic Foci | 0.196477* | 0.189971* | 0.103276 | 0.075765 | 0.296464* | 0.206360* | 0.130960 | 0.218441* | 0.254299* | 0.204143* | 0.114573 | 0.113438 |
Eisosomes | 0.000232 | 0.000217 | 0.000235 | 0.000189 | 0.000190 | 0.000207 | 0.000161 | 0.000137 | 0.000208 | 0.000208 | 0.000204 | 0.000176 |
Endoplasmic Reticulum | 0.001199 | 0.001569 | 0.005356 | 0.005404 | 0.002224 | 0.000541 | 0.004525 | 0.000452 | 0.001792 | 0.000680 | 0.005017 | 0.004096 |
Endosome | 0.071685 | 0.119939 | 0.018742 | 0.014044 | 0.062941 | 0.100885 | 0.022682 | 0.026144 | 0.066628 | 0.103463 | 0.020350 | 0.017239 |
Golgi | 0.069379 | 0.092741 | 0.001155 | 0.003983 | 0.052133 | 0.099403 | 0.002435 | 0.023722 | 0.059406 | 0.098502 | 0.001677 | 0.009195 |
Lipid Particles | 0.018402 | 0.004250 | 0.016457 | 0.004100 | 0.024836 | 0.011989 | 0.006945 | 0.001200 | 0.022122 | 0.010942 | 0.012575 | 0.003334 |
Mitochondria | 0.386376* | 0.239320* | 0.003624 | 0.009790 | 0.191781* | 0.300843* | 0.001883 | 0.010326 | 0.273842* | 0.292520* | 0.002913 | 0.009932 |
Mitotic Spindle | 0.010873 | 0.036280 | 0.009213 | 0.032015 | 0.013096 | 0.015829 | 0.015809 | 0.022248 | 0.012159 | 0.018596 | 0.011905 | 0.029436 |
None | 0.001727 | 0.000327 | 0.007108 | 0.002410 | 0.005982 | 0.001872 | 0.003157 | 0.006051 | 0.004187 | 0.001663 | 0.005496 | 0.003371 |
Nuclear Periphery | 0.000468 | 0.000176 | 0.003006 | 0.001407 | 0.001157 | 0.000366 | 0.001926 | 0.000145 | 0.000867 | 0.000340 | 0.002565 | 0.001074 |
Nuclear Periphery Foci | 0.000281 | 0.000332 | 0.002559 | 0.002079 | 0.000586 | 0.000354 | 0.002300 | 0.001094 | 0.000457 | 0.000351 | 0.002453 | 0.001819 |
Nucleolus | 0.001966 | 0.000584 | 0.009996 | 0.001192 | 0.007359 | 0.002655 | 0.002397 | 0.001538 | 0.005084 | 0.002375 | 0.006895 | 0.001283 |
Nucleus | 0.011314 | 0.005767 | 0.509351* | 0.214215* | 0.049533 | 0.011257 | 0.352660* | 0.037665 | 0.033416 | 0.010514 | 0.445410* | 0.167597* |
Peroxisomes | 0.080502 | 0.128244* | 0.013201 | 0.035094 | 0.042327 | 0.085124 | 0.033030 | 0.082158 | 0.058425 | 0.090958 | 0.021293 | 0.047521 |
Vacuole | 0.022759 | 0.043420 | 0.079741 | 0.159126 | 0.039718 | 0.036074 | 0.127174 | 0.088985 | 0.032566 | 0.037068 | 0.099097 | 0.140605 |
Vacuole Periphery | 0.010499 | 0.008321 | 0.001770 | 0.003596 | 0.013782 | 0.010889 | 0.000939 | 0.001382 | 0.012398 | 0.010541 | 0.001431 | 0.003011 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.14 | -1.96 | -6.24 | -0.89 | -5.55 | -4.23 | -2.45 | -3.58 | -1.68 | -0.64 | -4.80 | -3.36 | -7.11 | -4.71 | -5.16 |
Bud Neck | 0.65 | 7.13 | 3.73 | 1.91 | -5.83 | 0.54 | 8.05 | 4.24 | 3.91 | -3.08 | 0.99 | 11.00 | 5.62 | 4.57 | -6.58 |
Bud Site | -5.01 | 6.39 | -7.70 | 0.03 | -12.09 | -0.44 | 2.86 | -5.50 | -5.35 | -6.60 | -3.04 | 7.21 | -9.28 | -7.09 | -13.11 |
Cell Periphery | 0.97 | 1.18 | 1.53 | 0.48 | 0.70 | 0.76 | 0.99 | 5.64 | 5.96 | 4.28 | 2.18 | 1.00 | 1.98 | 0.02 | 1.37 |
Cytoplasm | 2.13 | -13.70 | -30.00 | -28.51 | -9.28 | 13.24 | -7.29 | -17.71 | -24.23 | -9.37 | 11.02 | -13.00 | -30.18 | -39.28 | -12.18 |
Cytoplasmic Foci | 0.68 | 11.49 | 21.06 | 12.38 | 6.72 | 12.13 | 18.92 | 7.78 | -0.20 | -8.09 | 8.39 | 23.36 | 27.73 | 19.80 | 0.97 |
Eisosomes | 0.53 | -0.03 | 4.74 | 1.69 | 3.86 | -1.20 | 2.05 | 3.78 | 4.46 | 1.85 | -0.06 | 0.38 | 5.25 | 4.24 | 3.85 |
Endoplasmic Reticulum | -0.85 | -8.00 | -9.90 | -7.56 | 1.17 | 4.50 | -6.42 | 4.54 | 0.59 | 12.03 | 4.37 | -9.53 | -7.10 | -12.28 | 3.67 |
Endosome | -4.50 | 12.53 | 16.30 | 11.12 | 4.32 | -8.90 | 11.96 | 11.04 | 19.00 | -1.35 | -9.52 | 17.65 | 22.38 | 27.29 | 3.17 |
Golgi | -2.55 | 18.20 | 17.74 | 11.39 | -2.14 | -10.69 | 18.12 | 8.26 | 17.59 | -5.59 | -9.96 | 25.72 | 21.86 | 27.56 | -5.72 |
Lipid Particles | 6.62 | 0.90 | 7.53 | 0.54 | 7.68 | 6.69 | 10.39 | 13.97 | 12.02 | 9.67 | 7.44 | 6.22 | 14.77 | 9.68 | 9.10 |
Mitochondria | 7.32 | 34.35* | 34.08* | 13.29 | -2.88 | -10.92 | 26.99 | 25.28 | 39.04* | -3.53 | -1.98 | 41.46* | 40.67* | 42.13* | -4.89 |
Mitotic Spindle | -4.23 | 0.07 | -4.19 | 1.85 | -3.65 | -1.31 | -0.97 | -1.40 | -0.81 | -0.32 | -3.41 | -0.53 | -4.24 | -1.65 | -2.89 |
None | 2.31 | -3.24 | -0.27 | -7.10 | 3.55 | 4.07 | 3.32 | -0.44 | -3.71 | -3.13 | 3.74 | -0.61 | 1.28 | -3.38 | 1.86 |
Nuclear Periphery | 1.44 | -7.01 | -7.94 | -12.90 | 3.01 | 2.28 | -3.08 | 2.94 | 1.88 | 11.19 | 2.35 | -6.55 | -3.20 | -9.92 | 5.26 |
Nuclear Periphery Foci | -0.47 | -5.58 | -5.51 | -4.95 | 2.21 | 2.83 | -7.65 | -3.49 | -4.81 | 4.17 | 1.49 | -7.45 | -5.71 | -6.11 | 3.02 |
Nucleolus | 2.69 | -5.58 | 1.98 | -2.02 | 6.80 | 4.63 | 5.30 | 5.73 | 1.75 | 0.72 | 3.96 | -1.35 | 6.68 | 3.37 | 6.90 |
Nucleus | 2.83 | -35.18 | -35.13 | -36.27 | 16.30 | 11.25 | -19.60 | 1.94 | -6.75 | 19.99 | 10.23 | -38.04 | -27.69 | -33.28 | 21.06 |
Peroxisomes | -3.70 | 13.20 | 11.39 | 8.85 | -2.58 | -10.09 | 3.33 | -4.04 | 1.92 | -5.64 | -7.69 | 12.11 | 6.79 | 13.06 | -4.86 |
Vacuole | -3.88 | -11.26 | -30.67 | -20.09 | -15.52 | 1.19 | -11.93 | -9.57 | -10.06 | 4.60 | -2.20 | -15.47 | -30.40 | -28.74 | -9.19 |
Vacuole Periphery | 1.70 | 8.95 | 7.99 | 5.61 | -0.77 | 2.25 | 13.16 | 12.18 | 11.76 | -1.03 | 1.89 | 15.36 | 13.38 | 11.24 | -1.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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