Standard name
Human Ortholog
Description Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0.06 0 0 0 0 0.05 0 0 0.1 0.12 0.1 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0 0 0.12 0.23 0.22 0.39 0.34 0.52 0.27 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.93 0.93 0.96 0.92 0.95 0.89 0.83 0.85 0.81 0.85 0.75 0.85 0.94 0.88 0.85 0.81 0.87 0.87 0.9 0.87 0.89 0.84 0.78 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.18 0.06 0 0 0.05 0.12 0.07 0.15 0.12 0.19 0.09 0 0 0 0 0 0.08 0.07 0.07 0.05 0 0.09 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0 0 0 0 0.05 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 3 0 3 3 4 1 0 0 0 0 0 0 0 1 1 0 2 1
Bud Neck 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 3 4 6 12 7 14 13 10 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 2 14 13 21 23 16 5 5 15 6 12 35 61 13 3 4 0 0 1 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1 0 0 2 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1
Mitochondria 43 2 8 2 4 47 120 95 135 121 153 123 2 0 0 1 3 4 1 0 0 1 8 7
Nucleus 257 92 183 180 315 338 433 364 283 298 219 389 261 305 416 110 54 72 245 90 184 172 327 322
Nuclear Periphery 0 0 0 3 0 2 7 2 6 1 5 2 0 3 3 1 0 0 0 0 0 1 0 1
Nucleolus 46 18 12 6 10 18 62 32 54 42 56 41 9 7 22 6 2 7 19 6 9 8 37 50
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 3 0 5 3 1 2 1 6 2 5 5 0 0 0 0 0 1 1 2 3
Vac/Vac Membrane 0 0 1 1 2 0 0 6 0 3 2 1 1 1 7 8 6 3 3 3 5 10 31 44
Unique Cell Count 276 99 191 196 333 379 521 429 349 351 292 457 279 345 489 135 62 83 273 104 207 204 419 442
Labelled Cell Count 349 115 207 207 350 430 661 530 494 489 469 579 288 356 518 143 68 92 273 104 207 204 419 442


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 8.5 6.8 6.5 6.8 5.7 5.1 5.4 4.6 4.6 4.1 4.9 7.3 7.5 7.5 11.2 10.1 10.0 7.0 7.2 7.9
Std Deviation (1e-4) 0.8 1.5 1.3 1.4 1.4 1.7 1.4 1.3 1.3 1.1 1.3 1.0 1.2 1.6 1.8 2.8 3.0 2.8 1.8 1.4 1.4
Intensity Change (Log2) – – – -0.06 -0.0 -0.25 -0.4 -0.32 -0.58 -0.55 -0.73 -0.45 0.1 0.15 0.15 0.72 0.58 0.56 0.04 0.09 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.0 1.9 2.6 2.2 1.8 0 0 2.4 0 2.0 4.0 4.6 3.6 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 5.8 5.5 0 0 0 6.6 0 0 0 0 0 0
Nucleus -1.6 -0.6 -2.7 -4.4 -3.9 -4.8 -3.8 -6.0 -3.9 -1.1 -2.9 -3.9 -4.2 -2.4 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -1.8 -0.8 2.2 0.5 0 0 0 1.1 -1.6 -2.6 -1.0 -0.7 0 0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8721 2.0785 1.7458 1.7031 1.337 1.7228 2.2002 2.3918 2.1661 1.7903 1.7657 2.1888 1.6236 1.819 1.2025 0.8518 0.8613 1.548
Actin 0.0016 0 0.0008 0.0002 0 0 0.0426 0 0.0001 0.0008 0 0 0.0408 0 0.0085 0 0.1503 0.0001
Bud 0.0006 0 0.0001 0.0001 0 0 0.0009 0 0.0001 0.0001 0 0 0.0006 0 0.0001 0 0.0014 0
Bud Neck 0.0006 0 0.0004 0.0004 0.0001 0.0004 0.008 0 0.0002 0.0008 0.0003 0.0005 0.0012 0 0.0001 0 0.0014 0.0007
Bud Periphery 0.0006 0 0.0004 0.0002 0 0 0.0014 0 0 0.0002 0.0002 0 0.0018 0 0.0001 0 0.0029 0
Bud Site 0.0004 0 0.0003 0.0003 0 0 0.0206 0 0.0011 0.0006 0.0001 0 0.0011 0 0.0005 0 0.0023 0
Cell Periphery 0.0003 0 0.0002 0.0002 0 0 0.0004 0 0 0.0001 0 0 0.0003 0 0 0 0.0003 0
Cytoplasm 0.0045 0 0.0007 0.0029 0 0.0002 0.0049 0 0.008 0.0036 0.0001 0 0.0153 0 0.0026 0 0.005 0
Cytoplasmic Foci 0.0016 0 0.0023 0.0045 0 0 0.023 0 0.0078 0.008 0 0 0.0072 0 0.0069 0 0.0165 0.0035
Eisosomes 0 0 0 0 0 0 0.0004 0 0 0 0 0 0.0002 0 0.0001 0 0.001 0
Endoplasmic Reticulum 0.0016 0 0.0024 0.0022 0 0 0.0042 0 0.0003 0.0014 0 0 0.0043 0 0.0003 0 0.0074 0
Endosome 0.0078 0 0.004 0.0071 0 0 0.0292 0 0.0073 0.0349 0 0 0.0069 0 0.0009 0 0.0523 0.0002
Golgi 0.0013 0 0.0003 0.0004 0 0 0.0102 0 0.0009 0.0125 0 0 0.0032 0 0.0005 0 0.0165 0.0001
Lipid Particles 0.0087 0 0.004 0.0037 0 0 0.0178 0 0.0077 0.0101 0 0 0.0152 0 0.001 0 0.0619 0.0009
Mitochondria 0.0036 0 0.002 0.0005 0.0004 0 0.0083 0.0001 0.0011 0.005 0.0011 0.0001 0.0017 0.0001 0.0034 0.0001 0.0135 0.0001
None 0.0014 0 0.0002 0.0018 0 0 0.0144 0 0.0005 0.0029 0.0001 0 0.0014 0 0.0067 0 0.0111 0.0001
Nuclear Periphery 0.0147 0.0004 0.007 0.0128 0.0025 0.0004 0.0143 0.0004 0.0016 0.0138 0.0027 0.0002 0.0094 0.0003 0.0008 0.0002 0.0338 0.0002
Nucleolus 0.0216 0.0211 0.0141 0.0145 0.0652 0.0537 0.028 0.0178 0.006 0.0162 0.1141 0.0645 0.0179 0.0174 0.0097 0.0136 0.0432 0.0532
Nucleus 0.9152 0.9734 0.9502 0.9116 0.9284 0.9432 0.7492 0.9812 0.9545 0.8718 0.88 0.934 0.8514 0.982 0.9481 0.9859 0.5229 0.9295
Peroxisomes 0.0025 0 0.0001 0.0003 0 0 0.007 0 0.0008 0.0017 0 0 0.0111 0 0.0073 0 0.0367 0.0016
Punctate Nuclear 0.0068 0.0049 0.0074 0.0328 0.0029 0.0018 0.0087 0.0002 0.0006 0.0121 0.0008 0.0004 0.0067 0.0001 0.0019 0.0002 0.0116 0.0095
Vacuole 0.003 0 0.0021 0.0024 0.0001 0.0001 0.0039 0.0001 0.0008 0.0025 0.0002 0.0001 0.0015 0 0.0002 0 0.0033 0.0001
Vacuole Periphery 0.0016 0 0.001 0.0009 0.0001 0 0.0025 0 0.0003 0.001 0.0002 0 0.0007 0 0.0001 0 0.0046 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.362 14.9938 10.3313 14.7997 20.9699 13.1713 18.4088 16.4445 15.451 19.9363
Translational Efficiency 0.788 1.0961 1.13 0.7187 0.7407 1.5788 1.0078 0.9797 1.0359 1.0299

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1989 394 164 125 1761 1821 293 1403 3750 2215 457 1528

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 858.89 974.10 1095.09 1154.96 852.33 943.45 1123.54 1017.21 855.81 948.90 1113.33 1028.48
Standard Deviation 133.20 159.91 125.38 171.19 123.00 138.22 144.99 155.27 128.55 142.80 138.95 161.12
Intensity Change Log 2 — 0.181597 0.350504 0.427298 — 0.146534 0.398567 0.255134 — 0.164239 0.374644 0.344112

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000383 0.000728 0.001545 0.001690 0.000274 0.000686 0.001226 0.001429 0.000332 0.000693 0.001341 0.001450
Bud Neck 0.005137 0.008661 0.008797 0.011117 0.005505 0.007974 0.008117 0.035276 0.005310 0.008096 0.008361 0.033300
Bud Site 0.000664 0.000714 0.001266 0.001280 0.000421 0.000928 0.000907 0.008873 0.000550 0.000890 0.001036 0.008252
Cell Periphery 0.000090 0.000042 0.000050 0.000053 0.000052 0.000080 0.000031 0.000246 0.000072 0.000073 0.000038 0.000230
Cytoplasm 0.001812 0.000154 0.000318 0.000730 0.000159 0.000586 0.000103 0.013827 0.001036 0.000509 0.000180 0.012756
Cytoplasmic Foci 0.000235 0.000254 0.000029 0.000035 0.000021 0.000915 0.000026 0.004486 0.000135 0.000797 0.000027 0.004122
Eisosomes 0.000027 0.000035 0.000064 0.000051 0.000029 0.000047 0.000057 0.000042 0.000028 0.000045 0.000059 0.000043
Endoplasmic Reticulum 0.003423 0.003083 0.004586 0.004687 0.002768 0.003574 0.003711 0.005196 0.003115 0.003487 0.004025 0.005154
Endosome 0.000198 0.000166 0.000115 0.000125 0.000088 0.000710 0.000079 0.005911 0.000146 0.000613 0.000092 0.005437
Golgi 0.000126 0.000264 0.000150 0.000126 0.000046 0.000701 0.000104 0.005845 0.000089 0.000623 0.000121 0.005377
Lipid Particles 0.000108 0.000196 0.000112 0.000124 0.000061 0.000410 0.000089 0.000465 0.000086 0.000372 0.000097 0.000437
Mitochondria 0.000740 0.003495 0.001218 0.002385 0.000526 0.001981 0.001020 0.007785 0.000639 0.002250 0.001091 0.007343
Mitotic Spindle 0.001737 0.003827 0.000512 0.000589 0.002502 0.004043 0.000228 0.058130 0.002096 0.004004 0.000330 0.053423
None 0.004349 0.001566 0.003199 0.005913 0.000998 0.001618 0.002064 0.007174 0.002775 0.001609 0.002471 0.007071
Nuclear Periphery 0.000180 0.000243 0.000161 0.000389 0.000173 0.000357 0.000120 0.004297 0.000176 0.000337 0.000135 0.003978
Nuclear Periphery Foci 0.000437 0.001122 0.000107 0.000064 0.000130 0.000425 0.000063 0.004230 0.000293 0.000549 0.000079 0.003889
Nucleolus 0.035869 0.037281 0.038395 0.018920 0.035160 0.034712 0.035078 0.017921 0.035536 0.035169 0.036268 0.018003
Nucleus 0.943649* 0.936716* 0.936736* 0.948716* 0.950497* 0.938588* 0.945067* 0.797678* 0.946865* 0.938255* 0.942077* 0.810034*
Peroxisomes 0.000322 0.000851 0.001435 0.001010 0.000268 0.000769 0.001060 0.000763 0.000297 0.000784 0.001195 0.000784
Vacuole 0.000278 0.000217 0.001049 0.001803 0.000190 0.000549 0.000737 0.013818 0.000236 0.000490 0.000849 0.012835
Vacuole Periphery 0.000235 0.000385 0.000154 0.000193 0.000131 0.000348 0.000113 0.006609 0.000186 0.000355 0.000128 0.006084

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.01 -9.97 -10.07 -7.09 -1.16 -3.43 -13.10 -18.20 -7.65 -3.29 -3.51 -15.64 -18.43 -8.95 -2.36
Bud Neck -3.14 -5.32 -7.31 -3.35 -3.19 -4.30 -4.56 -17.49 -16.18 -14.16 -5.66 -6.72 -18.09 -16.23 -14.64
Bud Site -0.54 -5.15 -5.01 -3.83 -1.00 -2.85 -5.28 -9.80 -8.89 -8.81 -2.48 -7.06 -9.84 -9.09 -8.54
Cell Periphery 3.12 2.53 1.81 -1.97 -1.11 -1.16 0.74 -3.87 -3.40 -5.65 -0.04 2.47 -3.68 -3.70 -5.46
Cytoplasm 3.12 2.85 1.89 -2.01 -1.46 -2.08 0.63 -7.47 -7.00 -7.50 1.47 3.00 -6.57 -7.09 -7.47
Cytoplasmic Foci 0.04 2.12 2.04 1.49 -1.29 -2.60 -0.94 -3.38 -1.52 -3.37 -2.22 2.08 -3.10 -1.64 -3.37
Eisosomes -3.09 -9.53 -6.53 -4.68 1.14 -4.31 -9.75 -9.14 -0.22 3.64 -4.95 -13.66 -11.48 -1.14 4.95
Endoplasmic Reticulum 0.93 -3.36 -3.35 -3.79 -0.20 -3.90 -4.38 -8.81 -6.30 -4.28 -1.99 -4.93 -8.20 -6.93 -3.73
Endosome 0.24 1.51 0.22 -0.13 -1.80 -2.77 -1.03 -6.17 -4.29 -6.14 -2.52 1.52 -6.03 -4.49 -6.11
Golgi -1.21 -1.15 -1.33 0.70 -0.41 -1.93 -7.59 -2.86 -1.98 -2.76 -1.90 -2.33 -2.82 -2.05 -2.74
Lipid Particles -0.91 -0.55 -1.01 0.53 -0.66 -2.43 -1.52 -6.14 -0.06 -5.78 -2.37 -1.01 -5.70 -0.17 -5.62
Mitochondria -1.41 -3.74 -4.61 0.19 -3.44 -2.45 -7.83 -6.56 -3.65 -5.67 -2.68 -6.43 -6.66 -3.33 -5.79
Mitotic Spindle -1.04 1.53 0.34 1.20 -1.39 -0.75 2.13 -8.88 -8.52 -9.82 -1.43 2.71 -9.17 -8.48 -9.79
None 4.00 1.20 0.24 -0.88 -0.40 -1.56 -1.89 -8.38 -5.32 -5.15 2.00 0.93 -4.30 -5.58 -4.26
Nuclear Periphery -0.44 -1.03 -4.82 -2.16 -3.98 -0.96 0.24 -6.86 -5.92 -6.89 -1.03 -0.45 -6.95 -6.10 -6.92
Nuclear Periphery Foci -0.83 1.73 2.11 1.13 0.80 -1.71 0.67 -3.22 -2.65 -3.28 -1.14 2.04 -2.88 -2.20 -3.23
Nucleolus -0.40 -1.16 2.93 2.69 2.82 0.25 -0.71 6.49 6.59 4.45 0.27 -1.25 8.23 7.24 5.58
Nucleus 1.24 2.16 0.96 -0.20 -0.86 3.29 2.53 16.50 14.27 13.70 2.85 2.99 16.20 14.11 13.30
Peroxisomes -2.66 -6.86 -4.69 -0.96 1.83 -2.77 -8.01 -4.97 0.79 4.18 -3.16 -10.22 -5.25 0.79 5.66
Vacuole 0.46 -4.58 -5.42 -5.54 -2.78 -2.63 -5.35 -7.58 -7.34 -7.09 -2.39 -6.95 -7.69 -7.50 -7.08
Vacuole Periphery -0.57 0.85 0.06 0.61 -1.99 -3.38 0.09 -4.39 -4.04 -4.40 -2.21 1.08 -4.30 -4.01 -4.38
Standard name
Human Ortholog
Description Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Kin28

Kin28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kin28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available