Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 8 | 6 | 7 | 17 | 7 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 2 | 4 | 3 | 14 | 13 | 17 | 14 | 20 | 22 | 12 | 2 | 6 | 1 | 5 | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 219 | 459 | 182 | 118 | 100 | 261 | 311 | 212 | 207 | 171 | 140 | 139 | 147 | 311 | 230 | 392 | 390 | 336 | 201 | 438 | 155 | 187 | 172 | 208 |
Endoplasmic Reticulum | 2 | 9 | 1 | 3 | 9 | 29 | 75 | 51 | 33 | 40 | 23 | 40 | 1 | 4 | 5 | 17 | 27 | 10 | 5 | 3 | 10 | 10 | 10 | 11 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 2 | 0 | 0 | 0 | 2 | 5 | 26 | 5 | 17 | 17 | 16 | 1 | 1 | 1 | 4 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 10 | 3 | 10 | 7 | 0 | 1 | 0 | 13 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 7 | 1 | 1 | 3 | 8 | 0 | 1 | 1 | 7 | 11 | 14 | 0 | 6 | 3 | 2 | 3 | 2 |
Unique Cell Count | 221 | 468 | 185 | 121 | 109 | 299 | 393 | 292 | 250 | 238 | 201 | 211 | 150 | 315 | 238 | 414 | 414 | 357 | 210 | 456 | 173 | 208 | 192 | 237 |
Labelled Cell Count | 223 | 476 | 187 | 126 | 113 | 308 | 412 | 324 | 276 | 260 | 232 | 230 | 151 | 324 | 239 | 442 | 449 | 379 | 210 | 456 | 173 | 208 | 192 | 237 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 90.9 | 67.2 | 71.8 | 63.5 | 37.1 | 42.0 | 37.1 | 32.6 | 31.1 | 31.6 | 29.7 | 29.6 | 71.0 | 67.3 | 62.7 | 82.6 | 102.1 | 103.3 | 79.4 | 74.8 | 72.8 |
Std Deviation (1e-4) | 15.5 | 12.4 | 16.5 | 13.7 | 12.7 | 7.9 | 7.1 | 7.2 | 6.5 | 6.4 | 6.6 | 6.3 | 19.6 | 15.3 | 16.0 | 33.6 | 34.9 | 33.7 | 22.0 | 23.4 | 25.0 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.95 | -0.77 | -0.95 | -1.14 | -1.21 | -1.19 | -1.27 | -1.28 | -0.02 | -0.09 | -0.2 | 0.2 | 0.51 | 0.52 | 0.15 | 0.06 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.2 | 0 | 2.6 | 2.5 | 3.4 | 4.0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -2.8 | -4.3 | -6.1 | -7.2 | -5.2 | -7.3 | -7.6 | -8.2 | -0.3 | 0.3 | -1.1 | -2.1 | -2.3 | -2.3 |
Endoplasmic Reticulum | 0 | 0 | 4.1 | 6.2 | 5.8 | 4.9 | 5.6 | 4.4 | 6.0 | 0 | 0 | 0 | 2.4 | 3.2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 4.2 | 0 | 3.7 | 4.0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 64.8071 | 83.3438 | 76.9755 | 73.4064 | 55.2698 | 75.9189 | 87.8714 | 110.8106 | 100.2103 | 97.5834 | 87.2656 | 99.0783 | 78.0708 | 93.9676 | 87.1596 | 83.8963 | 71.5479 | 81.5266 |
Actin | 0.0234 | 0.0003 | 0.0115 | 0.0012 | 0.0011 | 0.0065 | 0.0039 | 0.0002 | 0.0042 | 0.0003 | 0.0011 | 0.0009 | 0.0009 | 0.0009 | 0.0038 | 0.0095 | 0.0011 | 0.0071 |
Bud | 0.0018 | 0.0004 | 0.0011 | 0.0016 | 0.0007 | 0.0013 | 0.0016 | 0.0005 | 0.0012 | 0.0012 | 0.0016 | 0.0009 | 0.0014 | 0.0004 | 0.001 | 0.0018 | 0.0015 | 0.001 |
Bud Neck | 0.0016 | 0.0007 | 0.0018 | 0.0016 | 0.001 | 0.0014 | 0.0009 | 0.0006 | 0.0008 | 0.001 | 0.0012 | 0.0027 | 0.0008 | 0.0008 | 0.0009 | 0.0016 | 0.0007 | 0.0034 |
Bud Periphery | 0.0028 | 0.0004 | 0.0011 | 0.0014 | 0.0004 | 0.0012 | 0.0026 | 0.0002 | 0.0015 | 0.0007 | 0.0018 | 0.0008 | 0.002 | 0.0004 | 0.0018 | 0.0013 | 0.0009 | 0.0016 |
Bud Site | 0.0059 | 0.0003 | 0.0009 | 0.0004 | 0.0008 | 0.0004 | 0.0021 | 0.0002 | 0.0019 | 0.0003 | 0.0005 | 0.0002 | 0.0007 | 0.0027 | 0.0003 | 0.0019 | 0.0004 | 0.0005 |
Cell Periphery | 0.0166 | 0.0129 | 0.0121 | 0.0116 | 0.0021 | 0.0033 | 0.0168 | 0.0085 | 0.0249 | 0.0192 | 0.0118 | 0.006 | 0.0181 | 0.012 | 0.0144 | 0.0258 | 0.0181 | 0.0023 |
Cytoplasm | 0.5925 | 0.9047 | 0.7657 | 0.6212 | 0.6932 | 0.7746 | 0.6786 | 0.9172 | 0.7903 | 0.7124 | 0.7545 | 0.821 | 0.7101 | 0.8934 | 0.8055 | 0.6269 | 0.6604 | 0.7566 |
Cytoplasmic Foci | 0.013 | 0.0012 | 0.0081 | 0.0033 | 0.0988 | 0.0206 | 0.0075 | 0.001 | 0.0068 | 0.0135 | 0.0046 | 0.0079 | 0.0092 | 0.0019 | 0.0036 | 0.0059 | 0.0219 | 0.0116 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0 | 0.0006 | 0.0003 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.092 | 0.0244 | 0.0356 | 0.0033 | 0.0214 | 0.0247 | 0.0874 | 0.0187 | 0.0328 | 0.0211 | 0.037 | 0.0707 | 0.0703 | 0.0327 | 0.0396 | 0.0136 | 0.0058 | 0.0787 |
Endosome | 0.0185 | 0.0035 | 0.0179 | 0.0117 | 0.0152 | 0.0297 | 0.0104 | 0.0026 | 0.0061 | 0.0123 | 0.0138 | 0.0184 | 0.012 | 0.0049 | 0.0083 | 0.014 | 0.0145 | 0.035 |
Golgi | 0.0063 | 0.0008 | 0.0027 | 0.0012 | 0.0047 | 0.0092 | 0.0013 | 0.0003 | 0.0017 | 0.0005 | 0.001 | 0.0037 | 0.0015 | 0.0023 | 0.0065 | 0.0016 | 0.0012 | 0.0059 |
Lipid Particles | 0.0076 | 0.001 | 0.0034 | 0.0013 | 0.0283 | 0.0207 | 0.0042 | 0.0007 | 0.0032 | 0.0053 | 0.0032 | 0.0018 | 0.0043 | 0.0013 | 0.0022 | 0.0023 | 0.0083 | 0.0073 |
Mitochondria | 0.0159 | 0.0007 | 0.0026 | 0.0007 | 0.0158 | 0.0162 | 0.006 | 0.0003 | 0.0061 | 0.0018 | 0.01 | 0.0052 | 0.007 | 0.0046 | 0.0059 | 0.0013 | 0.0106 | 0.0255 |
None | 0.0034 | 0.0002 | 0.0062 | 0.0003 | 0.0181 | 0.0047 | 0.0047 | 0.0002 | 0.0035 | 0.0026 | 0.004 | 0.0007 | 0.0036 | 0.0002 | 0.0007 | 0.0017 | 0.0586 | 0.0015 |
Nuclear Periphery | 0.0308 | 0.0042 | 0.0082 | 0.0064 | 0.0141 | 0.0085 | 0.0234 | 0.0036 | 0.0073 | 0.0119 | 0.0135 | 0.0064 | 0.0182 | 0.0027 | 0.0058 | 0.0126 | 0.0063 | 0.0174 |
Nucleolus | 0.0058 | 0.0001 | 0.0037 | 0.0004 | 0.0141 | 0.0018 | 0.0037 | 0.0001 | 0.005 | 0.0064 | 0.0023 | 0.0019 | 0.0035 | 0.0001 | 0.0002 | 0.0006 | 0.0076 | 0.0011 |
Nucleus | 0.0105 | 0.0013 | 0.0047 | 0.0035 | 0.0049 | 0.002 | 0.0073 | 0.0014 | 0.0047 | 0.0054 | 0.0079 | 0.0025 | 0.0064 | 0.0013 | 0.0021 | 0.0042 | 0.0055 | 0.0047 |
Peroxisomes | 0.0068 | 0 | 0.0006 | 0.0001 | 0.0023 | 0.0024 | 0.003 | 0 | 0.0022 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0046 | 0.001 | 0.0007 | 0.0011 |
Punctate Nuclear | 0.0018 | 0 | 0.0007 | 0.0001 | 0.0179 | 0.0012 | 0.0012 | 0 | 0.0005 | 0.0029 | 0.0094 | 0.0003 | 0.0024 | 0.0001 | 0.0001 | 0.0056 | 0.0028 | 0.0021 |
Vacuole | 0.12 | 0.0395 | 0.092 | 0.2408 | 0.0341 | 0.0546 | 0.1109 | 0.0407 | 0.0844 | 0.1467 | 0.0907 | 0.0395 | 0.1068 | 0.0342 | 0.0808 | 0.2236 | 0.1262 | 0.0275 |
Vacuole Periphery | 0.0228 | 0.0034 | 0.0193 | 0.0879 | 0.0104 | 0.0149 | 0.0222 | 0.0032 | 0.0108 | 0.0338 | 0.0296 | 0.0083 | 0.02 | 0.0029 | 0.0115 | 0.0431 | 0.0467 | 0.0079 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2149.0549 | 2339.5865 | 2883.3751 | 2326.7146 | 2636.5826 | 2779.769 | 2037.9202 | 1993.4185 | 2222.9014 | 1978.0408 |
Translational Efficiency | 1.1117 | 1.1419 | 0.9497 | 1.1054 | 1.2728 | 0.8791 | 0.9148 | 1.2138 | 0.9694 | 1.1829 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|