Standard name | |
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Human Ortholog | |
Description | RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 1 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 16 | 1 | 8 | 8 | 3 | 9 | 13 | 14 | 15 | 8 | 7 | 7 | 9 | 16 | 17 | 25 | 18 | 20 | 0 | 1 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 4 | 3 | 4 | 10 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 7 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 3 | 5 | 5 | 5 | 1 | 6 | 7 | 19 | 27 | 43 | 32 | 32 | 1 | 2 | 5 | 13 | 7 | 17 | 0 | 1 | 1 | 0 | 0 | 1 |
Nucleus | 278 | 121 | 185 | 203 | 233 | 118 | 185 | 230 | 81 | 151 | 123 | 119 | 121 | 141 | 131 | 115 | 145 | 136 | 242 | 110 | 135 | 91 | 133 | 88 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 168 | 101 | 131 | 88 | 73 | 85 | 115 | 241 | 110 | 175 | 153 | 163 | 94 | 101 | 89 | 64 | 63 | 69 | 100 | 41 | 103 | 44 | 82 | 86 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 1 | 1 | 12 | 14 | 28 | 38 | 7 | 11 | 9 | 12 | 0 | 2 | 2 | 12 | 3 | 6 | 8 | 6 | 10 | 7 | 21 | 16 |
Unique Cell Count | 350 | 153 | 244 | 234 | 287 | 202 | 310 | 489 | 199 | 315 | 258 | 265 | 162 | 195 | 190 | 187 | 206 | 194 | 357 | 167 | 265 | 149 | 242 | 204 |
Labelled Cell Count | 467 | 229 | 330 | 305 | 326 | 237 | 354 | 560 | 244 | 396 | 329 | 338 | 230 | 270 | 247 | 236 | 245 | 252 | 357 | 167 | 265 | 149 | 242 | 204 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 42.0 | 28.4 | 21.9 | 21.7 | 28.3 | 29.8 | 30.1 | 30.2 | 28.0 | 27.0 | 26.2 | 24.4 | 35.6 | 33.1 | 28.6 | 33.2 | 38.6 | 37.2 | 31.1 | 32.0 | 34.6 |
Std Deviation (1e-4) | 16.5 | 11.8 | 8.0 | 6.8 | 7.1 | 8.7 | 8.7 | 8.9 | 10.4 | 8.2 | 9.0 | 8.5 | 10.9 | 12.1 | 10.4 | 18.1 | 15.8 | 14.9 | 8.7 | 10.2 | 13.8 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.37 | 0.45 | 0.46 | 0.47 | 0.36 | 0.3 | 0.26 | 0.16 | 0.7 | 0.6 | 0.39 | 0.6 | 0.82 | 0.77 | 0.51 | 0.55 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | -1.8 | 0.6 | 0.6 | -0.3 | 2.0 | -0.5 | -0.4 | -0.4 | 1.1 | 2.3 | 2.5 | 3.9 | 2.5 | 3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0.2 | 1.3 | 4.7 | 4.9 | 4.4 | 4.4 | 0 | 0 | 0 | 2.5 | 0.9 | 3.2 |
Nucleus | 3.1 | 1.5 | -3.9 | -4.0 | -7.4 | -7.5 | -6.7 | -6.5 | -7.1 | -0.3 | -0.8 | -1.6 | -3.2 | -1.3 | -1.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.5 | -6.7 | -2.4 | -3.9 | -1.1 | 0.3 | 0.4 | 1.3 | 1.8 | 0.9 | -0.4 | -1.4 | -4.0 | -4.9 | -3.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.8 | 3.8 | 4.5 | 4.2 | 0 | 2.5 | 0 | 2.9 | 0 | 0 | 0 | 3.6 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 27.9728 | 31.0872 | 30.3171 | 29.0182 | 22.6527 | 31.427 | 29.8087 | 37.3916 | 33.156 | 30.6333 | 32.632 | 34.5283 | 36.3483 | 40.174 | 34.8596 | 37.9677 | 33.3852 | 36.121 |
Actin | 0.0049 | 0.0006 | 0.0075 | 0 | 0.0117 | 0.0112 | 0.0302 | 0 | 0.0009 | 0 | 0.0419 | 0.0025 | 0.0044 | 0 | 0.0054 | 0 | 0.0002 | 0 |
Bud | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0007 | 0.0012 | 0.0005 | 0 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0097 | 0.0008 | 0.0004 | 0 | 0 | 0 | 0.0004 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0014 | 0.0034 | 0.0008 | 0 | 0 | 0.0001 | 0.002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Bud Site | 0.0008 | 0.0015 | 0.0008 | 0 | 0.0008 | 0.0018 | 0.0012 | 0 | 0.0001 | 0 | 0.0021 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0003 | 0 | 0.0002 | 0 | 0.0008 | 0.0007 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0019 | 0.0002 | 0.0008 | 0 | 0.0005 | 0.0002 | 0.0012 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 | 0.0017 | 0 |
Cytoplasmic Foci | 0.0025 | 0.0003 | 0.0016 | 0 | 0.0202 | 0.0004 | 0.0139 | 0 | 0.0006 | 0 | 0.0003 | 0.0057 | 0.0009 | 0 | 0.0048 | 0 | 0.0419 | 0.0013 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0019 | 0.0001 | 0.0012 | 0 | 0.0022 | 0.0003 | 0.0014 | 0 | 0.0003 | 0 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 | 0.0005 | 0 |
Endosome | 0.012 | 0.0006 | 0.0084 | 0 | 0.0259 | 0.0014 | 0.0119 | 0 | 0.0039 | 0 | 0.0014 | 0.0012 | 0.0018 | 0 | 0.0017 | 0 | 0.0076 | 0 |
Golgi | 0.0019 | 0.0002 | 0.0014 | 0 | 0.0151 | 0.0009 | 0.0071 | 0 | 0.0028 | 0 | 0.0014 | 0.0014 | 0.0016 | 0 | 0.0011 | 0 | 0.0007 | 0 |
Lipid Particles | 0.0031 | 0.0001 | 0.0027 | 0 | 0.0493 | 0.0003 | 0.01 | 0 | 0.0008 | 0 | 0.0005 | 0.0048 | 0.0017 | 0 | 0.0035 | 0 | 0.0119 | 0.0006 |
Mitochondria | 0.0038 | 0.0004 | 0.0049 | 0.0004 | 0.0648 | 0.0124 | 0.0071 | 0.0002 | 0.001 | 0.0007 | 0.0201 | 0.0031 | 0.0017 | 0.0002 | 0.0028 | 0.0001 | 0.0078 | 0.0011 |
None | 0.0018 | 0.0001 | 0.003 | 0 | 0.0011 | 0.0003 | 0.0014 | 0 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0016 | 0 | 0.0035 | 0.0001 |
Nuclear Periphery | 0.0135 | 0.0011 | 0.0047 | 0.0001 | 0.0115 | 0.0027 | 0.0259 | 0.0001 | 0.0062 | 0.0003 | 0.003 | 0.0003 | 0.0013 | 0.0001 | 0.0007 | 0.0001 | 0.001 | 0 |
Nucleolus | 0.1945 | 0.0862 | 0.0744 | 0.0416 | 0.3797 | 0.2585 | 0.0804 | 0.0525 | 0.0569 | 0.1114 | 0.3481 | 0.209 | 0.1062 | 0.075 | 0.0834 | 0.0382 | 0.4 | 0.2433 |
Nucleus | 0.7478 | 0.9031 | 0.8829 | 0.9577 | 0.3872 | 0.6977 | 0.7798 | 0.9471 | 0.9244 | 0.8869 | 0.5725 | 0.7684 | 0.8775 | 0.9246 | 0.888 | 0.9616 | 0.5144 | 0.7526 |
Peroxisomes | 0.0008 | 0.0001 | 0.0007 | 0 | 0.008 | 0.0006 | 0.0098 | 0 | 0.0001 | 0 | 0.0005 | 0.0009 | 0.0008 | 0 | 0.0031 | 0 | 0.0008 | 0.0001 |
Punctate Nuclear | 0.0052 | 0.0047 | 0.0026 | 0 | 0.0042 | 0.0012 | 0.0147 | 0 | 0.0006 | 0.0001 | 0.0016 | 0.0013 | 0.0008 | 0 | 0.001 | 0 | 0.0061 | 0.0001 |
Vacuole | 0.0012 | 0.0001 | 0.0006 | 0 | 0.0019 | 0.0027 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0008 | 0 | 0.003 | 0.0012 | 0.0014 | 0 | 0.0004 | 0.0002 | 0.0008 | 0.0003 | 0.0003 | 0 | 0.0007 | 0 | 0.0009 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 172.977 | 190.7525 | 181.7875 | 209.0006 | 155.7943 | 150.7278 | 207.1048 | 190.7321 | 184.464 | 192.7562 |
Translational Efficiency | 1.0367 | 0.9333 | 0.8554 | 0.675 | 0.746 | 1.0092 | 0.7715 | 0.5756 | 0.657 | 0.6364 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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