Standard name
Human Ortholog
Description Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.96 0.95 0.91 0.91 0.91 0.84 0.82 0.76 0.83 0.99 0.98 0.97 0.94 0.97 0.95 0.97 0.97 0.95 0.88 0.89 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.12 0.05 0.28 0.28 0.33 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 3 3
Bud 0 0 4 0 0 2 3 3 4 9 9 10 2 0 0 0 0 0 0 0 0 0 1 3
Bud Neck 0 0 0 0 0 1 1 0 2 1 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 28 6 7 5 9 5 4 4 6 2 2 1 1 4 6 3 0 0 0 0 0 0
Cytoplasm 241 483 607 474 486 571 610 666 503 478 415 478 406 470 457 98 124 63 232 482 630 239 408 331
Endoplasmic Reticulum 0 5 7 4 12 10 2 1 3 1 0 2 2 1 3 4 9 1 0 1 7 9 11 10
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 3 1 2 3
Golgi 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 2 2 0 5 3
Mitochondria 1 0 0 1 8 52 80 37 167 162 183 119 0 4 2 1 1 0 2 1 6 3 7 3
Nucleus 0 1 5 1 3 4 5 17 2 7 9 5 1 1 0 2 0 1 0 2 1 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 3 1 0 0 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
SpindlePole 0 0 0 0 0 0 2 2 1 2 2 8 0 0 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 0 2 0 11 13 15 5 40 14 17 11 3 2 7 15 4 1 0 0 3 4 5 8 10
Unique Cell Count 241 489 626 492 514 625 667 735 601 585 549 577 412 480 472 104 128 66 241 497 661 273 457 382
Labelled Cell Count 244 495 654 497 530 660 719 772 701 682 640 632 416 485 480 113 142 69 241 497 661 273 457 382


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 9.9 8.9 8.2 7.2 5.3 4.9 5.3 4.3 4.0 3.8 4.2 8.8 8.4 8.2 10.7 11.3 10.6 8.6 8.6 9.0
Std Deviation (1e-4) 1.3 1.4 1.0 1.2 1.1 0.9 0.9 1.0 0.9 0.9 0.7 0.9 1.3 1.3 1.2 1.8 1.8 1.9 1.7 1.2 1.5
Intensity Change (Log2) – – – -0.12 -0.29 -0.75 -0.85 -0.75 -1.06 -1.17 -1.24 -1.07 -0.02 -0.08 -0.12 0.26 0.35 0.25 -0.04 -0.04 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -3.1 -3.0 -4.1 -3.4 -4.5 0 0 0 0 -3.8 -4.4 -4.4 0 0.1 0
Cytoplasm -0.6 -2.0 -4.2 -4.2 -4.7 -7.9 -8.7 -10.9 -8.2 1.6 1.0 -0.1 -1.4 -0.1 -0.7
Endoplasmic Reticulum 0 1.6 0.7 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.4 8.9 5.7 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 2.2 0 0.7 1.3 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.0 3.9 0 5.9 3.8 4.3 3.6 0 0 0 4.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0391 5.2809 4.5231 3.8029 4.001 4.6708 5.9086 7.1986 6.3832 6.2608 6.0734 6.1236 3.7735 4.6171 4.3226 4.6497 3.8549 4.4694
Actin 0.0017 0.0004 0.0003 0.0005 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0006 0.0006 0.0009 0.0007 0.0008
Bud 0.0022 0.0005 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0002 0.0002 0.0001 0 0.0001 0.0002 0.0003 0.0001 0
Bud Neck 0.001 0.0004 0.0003 0.0003 0.0004 0.0011 0.0001 0.0001 0.0003 0.0005 0.0004 0.0006 0.0001 0.0001 0.0059 0.0003 0.0003 0.0004
Bud Periphery 0.0012 0.0002 0.0001 0 0 0.0001 0 0 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0 0
Bud Site 0.0052 0.0024 0.0004 0.0001 0.0001 0.0001 0.0002 0.0003 0.0007 0.0002 0.0001 0 0.0001 0.0004 0.0008 0.0002 0.0001 0
Cell Periphery 0.0001 0.0001 0 0 0 0.0002 0 0 0 0 0 0 0 0 0.0001 0.0001 0 0
Cytoplasm 0.9171 0.9439 0.9372 0.9308 0.9172 0.9308 0.9655 0.9574 0.9572 0.965 0.9789 0.9698 0.9353 0.9821 0.9525 0.9117 0.9754 0.9692
Cytoplasmic Foci 0.0098 0.0078 0.0073 0.0072 0.0099 0.0134 0.0045 0.0048 0.0073 0.0052 0.0035 0.0058 0.0032 0.0018 0.0033 0.0046 0.0064 0.0045
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0059 0.0032 0.004 0.0086 0.0038 0.0045 0.0032 0.0013 0.0038 0.0081 0.0034 0.0028 0.0054 0.0018 0.0051 0.0389 0.0043 0.0027
Endosome 0.011 0.0069 0.012 0.0097 0.0232 0.0091 0.0037 0.0062 0.0061 0.0042 0.003 0.0045 0.0026 0.0011 0.0098 0.0209 0.0058 0.005
Golgi 0.0004 0.0004 0.0004 0.0004 0.0005 0.0003 0.0002 0.0005 0.0004 0.0002 0.0001 0.0001 0.0002 0.0001 0.0003 0.0022 0.0005 0.001
Lipid Particles 0.0001 0.0001 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0 0.0007 0.0002 0 0.0013
Mitochondria 0.0004 0.0004 0.0004 0.0004 0.0005 0.0006 0.0003 0.0004 0.0004 0.0003 0.0002 0.0004 0.0001 0.0001 0.0002 0.0031 0.0002 0.0021
None 0.0331 0.0208 0.0276 0.0346 0.0369 0.0292 0.0174 0.0153 0.0162 0.0095 0.0048 0.0083 0.0485 0.0094 0.0091 0.0036 0.0025 0.009
Nuclear Periphery 0.002 0.0018 0.0019 0.0018 0.0009 0.0012 0.0008 0.0007 0.0009 0.001 0.0005 0.0008 0.0013 0.0005 0.003 0.0067 0.001 0.0007
Nucleolus 0.0001 0.0001 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0
Nucleus 0.0052 0.0074 0.0043 0.0031 0.0039 0.0049 0.0025 0.0113 0.0036 0.0033 0.0028 0.0051 0.0018 0.0011 0.0059 0.0022 0.0017 0.0015
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0.0001 0.0001 0.0009
Punctate Nuclear 0.0009 0.001 0.0006 0.0003 0.0002 0.0008 0.0002 0.0003 0.0005 0.0003 0.0001 0.0003 0.0002 0.0001 0.001 0.0002 0.0003 0.0003
Vacuole 0.0023 0.0019 0.0023 0.0015 0.0017 0.003 0.0008 0.0007 0.0014 0.0017 0.0013 0.0008 0.0007 0.0004 0.0011 0.0024 0.0005 0.0003
Vacuole Periphery 0.0003 0.0003 0.0004 0.0005 0.0005 0.0005 0.0001 0.0002 0.0002 0.0002 0.0003 0.0002 0.0001 0.0001 0.0002 0.0011 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.486 30.5959 27.4475 34.0763 34.4407 22.4643 26.8954 21.5027 19.439 37.9677
Translational Efficiency 0.8475 0.9728 1.0935 0.5656 0.7579 1.1185 0.926 0.7487 0.7598 0.6445

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
571 1561 1122 1191 1615 2157 1188 181 2186 3718 2310 1372

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 812.92 1068.37 1133.75 1147.63 921.91 1059.38 1164.40 1192.01 893.44 1063.15 1149.51 1153.48
Standard Deviation 96.83 157.73 186.18 162.09 120.37 141.65 156.87 173.24 124.28 148.68 172.41 164.29
Intensity Change Log 2 — 0.394226 0.479917 0.497472 — 0.200522 0.336889 0.370699 — 0.294534 0.405679 0.431492

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000361 0.000541 0.000548 0.000713 0.000236 0.000295 0.000226 0.003970 0.000268 0.000398 0.000382 0.001143
Bud Neck 0.005140 0.001571 0.002088 0.005068 0.001684 0.001896 0.001673 0.001880 0.002586 0.001760 0.001875 0.004648
Bud Site 0.016875 0.008557 0.012672 0.030092 0.008274 0.010578 0.005724 0.052422 0.010521 0.009730 0.009098 0.033038
Cell Periphery 0.000234 0.000067 0.000133 0.000121 0.000108 0.000057 0.000079 0.000106 0.000141 0.000061 0.000105 0.000119
Cytoplasm 0.581489* 0.735955* 0.634849* 0.623285* 0.703237* 0.748375* 0.737670* 0.677320* 0.671435* 0.743160* 0.687728* 0.630413*
Cytoplasmic Foci 0.279835* 0.114339 0.015441 0.023269 0.178075 0.125353 0.013505 0.018303 0.204656* 0.120729 0.014446 0.022614
Eisosomes 0.000087 0.000014 0.000021 0.000015 0.000026 0.000016 0.000012 0.000015 0.000042 0.000015 0.000016 0.000015
Endoplasmic Reticulum 0.003751 0.002004 0.003125 0.000794 0.003069 0.000810 0.001163 0.000542 0.003247 0.001311 0.002116 0.000761
Endosome 0.018099 0.010410 0.004225 0.014725 0.008422 0.007063 0.001593 0.000985 0.010950 0.008468 0.002872 0.012912
Golgi 0.008616 0.002937 0.000258 0.013924 0.004644 0.005840 0.000152 0.000224 0.005682 0.004621 0.000204 0.012117
Lipid Particles 0.004451 0.001703 0.001480 0.001592 0.002287 0.001268 0.000809 0.000776 0.002853 0.001451 0.001135 0.001485
Mitochondria 0.002397 0.002051 0.000386 0.007444 0.002386 0.001781 0.000184 0.000219 0.002389 0.001894 0.000282 0.006491
Mitotic Spindle 0.000304 0.004577 0.011916 0.077445 0.000812 0.004591 0.005246 0.007124 0.000679 0.004585 0.008485 0.068168
None 0.001490 0.001495 0.003816 0.004917 0.002337 0.001812 0.006286 0.006808 0.002116 0.001679 0.005086 0.005167
Nuclear Periphery 0.000118 0.000291 0.001362 0.001612 0.000100 0.000092 0.000596 0.000295 0.000105 0.000176 0.000968 0.001438
Nuclear Periphery Foci 0.001021 0.002418 0.007897 0.004799 0.001634 0.000895 0.003845 0.002379 0.001474 0.001534 0.005813 0.004480
Nucleolus 0.000268 0.000277 0.000222 0.000663 0.000230 0.000213 0.000133 0.000132 0.000240 0.000240 0.000176 0.000593
Nucleus 0.022180 0.023803 0.184832* 0.079389 0.026070 0.023568 0.146266* 0.112457 0.025054 0.023667 0.164998* 0.083751
Peroxisomes 0.001591 0.000889 0.000228 0.000924 0.000753 0.001213 0.000173 0.013278 0.000972 0.001077 0.000200 0.002554
Vacuole 0.048128 0.084145 0.114128 0.097920 0.053181 0.063297 0.074246 0.100683 0.051861 0.072050 0.093617 0.098284
Vacuole Periphery 0.003566 0.001956 0.000372 0.011290 0.002434 0.000985 0.000421 0.000081 0.002730 0.001393 0.000397 0.009811

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.57 -2.14 -1.21 -0.04 0.52 -1.60 -2.90 -1.39 -1.24 -1.22 -1.06 -4.63 -2.73 -1.15 -1.12
Bud Neck 6.11 3.97 3.13 -3.08 -0.34 -0.92 -0.30 -1.21 -0.91 -1.08 3.75 1.44 -0.70 -2.83 -1.56
Bud Site 4.18 2.90 1.24 -2.84 -1.71 -2.00 3.18 -1.47 -1.03 -2.05 1.00 2.80 -2.06 -2.58 -3.61
Cell Periphery 4.49 2.41 3.63 -3.85 4.64 7.07 1.87 -1.00 -6.27 -2.51 7.16 2.01 3.31 -6.15 2.39
Cytoplasm -12.28 4.39 3.11 18.36 -1.45 -5.52 6.96 8.92 11.05 5.83 -10.35 11.66 14.66 23.76 5.01
Cytoplasmic Foci 18.76 32.55 32.16 26.51 -2.23 9.76 36.81 33.09 27.95 -2.91 18.14 47.25 45.85 40.43 -3.49
Eisosomes 9.32 7.49 8.91 -2.83 7.57 5.74 4.85 1.32 -3.02 -2.26 10.79 7.63 9.50 -3.41 4.55
Endoplasmic Reticulum 2.11 0.47 3.96 4.32 10.79 5.76 4.44 6.36 1.06 5.60 5.22 2.46 7.65 4.42 12.66
Endosome 3.98 7.33 7.42 7.41 1.18 2.27 13.40 14.38 14.77 3.26 3.64 12.69 9.36 7.87 -0.43
Golgi 4.87 8.08 4.34 0.14 -2.76 -1.16 11.71 11.60 6.73 -1.98 1.79 13.94 3.82 2.26 -2.81
Lipid Particles 6.22 6.42 7.22 1.17 2.55 5.70 7.43 4.48 -0.77 -1.48 7.78 8.71 7.45 0.22 0.66
Mitochondria 0.41 3.20 0.91 0.35 -1.67 0.72 2.80 2.83 3.35 0.75 0.74 3.48 1.19 0.59 -1.80
Mitotic Spindle -3.68 -6.39 -8.31 -6.77 -4.80 -3.64 -5.65 -3.66 -2.89 -2.41 -4.99 -8.28 -8.79 -7.38 -5.91
None 0.12 -4.91 -5.56 -6.55 -1.92 0.95 -3.48 -3.50 -3.96 -2.17 1.08 -4.02 -4.54 -7.46 -1.34
Nuclear Periphery -2.04 -16.42 -5.48 -4.09 4.56 0.36 -21.05 -7.82 -7.84 1.37 -1.93 -22.79 -6.22 -5.61 2.77
Nuclear Periphery Foci -5.45 -11.73 -7.22 -1.54 8.07 5.55 -8.93 -2.95 -4.93 3.62 -0.73 -14.06 -6.43 -5.83 8.45
Nucleolus -0.30 0.85 -0.59 -0.46 -0.97 0.49 3.39 -0.25 -0.37 -1.06 -0.20 2.43 -0.91 -0.86 -1.50
Nucleus -1.08 -31.24 -21.91 -23.60 15.05 1.50 -30.91 -8.55 -8.79 4.84 0.78 -43.34 -24.53 -25.49 15.59
Peroxisomes 3.30 7.93 7.02 3.92 -1.54 -3.38 7.82 -0.65 -0.37 -1.03 -0.86 10.86 2.03 2.39 -1.54
Vacuole -9.09 -19.52 -25.93 -20.50 -5.91 -4.31 -15.91 -11.38 -10.76 -6.43 -9.43 -25.07 -28.98 -25.45 -10.46
Vacuole Periphery 2.63 5.47 1.51 -0.46 -2.85 3.97 5.65 6.48 12.36 1.22 4.20 7.72 1.20 -0.98 -2.83
Standard name
Human Ortholog
Description Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nrp1

Nrp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nrp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available