Standard name
Human Ortholog
Description Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.95 0.96 0.85 0.77 0.72 0.68 0.67 0.64 0.63 0.98 0.99 0.98 0.99 0.98 0.97 0.96 0.87 0.92 0.9 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.25 0.21 0.36 0.41 0.45 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.07 0.15 0.15 0.12 0.09 0.13 0 0 0.05 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2
Bud 0 0 0 0 0 1 9 5 9 14 5 9 1 1 0 0 0 1 4 1 7 13 17 26
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 7 2 0 5 11 9 7 7 6 6 1 1 0 2 8 2 0 0 0 0 0 0
Cytoplasm 273 159 310 272 377 318 449 371 285 309 225 251 561 616 633 334 474 340 256 139 296 343 402 435
Endoplasmic Reticulum 1 0 4 2 0 3 4 6 0 2 1 2 0 1 0 8 21 5 0 1 4 1 2 1
Endosome 0 0 0 0 0 0 0 4 1 1 0 1 0 0 3 0 1 0 0 0 3 2 0 3
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1 1 0 0
Mitochondria 1 2 0 5 14 64 148 111 151 189 159 166 0 0 0 1 3 1 0 0 2 1 2 3
Nucleus 0 0 2 2 1 2 9 8 6 5 5 2 2 0 1 0 2 2 0 9 0 4 7 2
Nuclear Periphery 0 0 1 0 0 1 2 3 6 2 1 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 2 4 5
Vac/Vac Membrane 1 1 6 9 10 19 41 75 62 54 30 50 10 18 34 4 5 17 0 0 1 4 1 3
Unique Cell Count 276 160 318 285 393 372 584 517 418 463 350 397 571 624 649 337 486 351 267 160 324 382 453 492
Labelled Cell Count 276 163 330 292 404 413 673 594 528 584 432 491 576 638 671 349 516 368 267 160 324 382 453 492


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 11.5 12.3 11.1 9.8 8.3 7.4 6.6 6.0 5.6 5.2 4.9 11.4 12.2 11.2 35.0 36.6 31.9 12.0 11.6 12.2
Std Deviation (1e-4) 4.3 4.7 4.2 4.1 3.0 3.8 3.5 2.5 2.7 2.2 2.3 2.2 4.6 5.0 4.5 11.9 11.4 12.0 4.3 4.5 4.7
Intensity Change (Log2) – – – -0.16 -0.33 -0.57 -0.73 -0.89 -1.03 -1.13 -1.25 -1.33 -0.11 -0.02 -0.14 1.51 1.57 1.37 -0.04 -0.09 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 -0.9 -0.3 -0.5 -0.5 -0.7 -0.5 -0.7 0 0 0 0 -0.6 0
Cytoplasm -1.4 -1.1 -5.5 -8.1 -9.3 -10.0 -10.4 -10.7 -11.1 0.8 1.4 0 1.6 0 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.1 2.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.4 7.8 9.8 8.9 12.0 13.1 13.8 13.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 0.6 2.3 3.3 6.0 6.0 5.0 3.8 5.3 -0.1 0.9 2.5 0 0 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.2367 15.9526 13.7791 11.8439 10.7267 14.126 10.0921 16.4977 13.748 10.2234 9.5411 12.8888 8.3841 11.2029 8.8128 9.3044 9.8114 9.245
Actin 0.0235 0.0029 0.008 0.049 0.0391 0.0032 0.0508 0.0001 0.0099 0.016 0.0321 0.0114 0.0176 0.0008 0.0144 0.0307 0.0003 0.0007
Bud 0.0039 0.0005 0.0043 0.0014 0.0003 0.0007 0.0015 0.0001 0.0006 0.0032 0.0027 0.0005 0.0006 0.0019 0.0022 0.0025 0.0001 0.0007
Bud Neck 0.0017 0.0003 0.0014 0.0007 0.001 0.0011 0.0023 0.0001 0.0012 0.0013 0.001 0.0011 0.0047 0.0003 0.0009 0.0019 0.0006 0.0007
Bud Periphery 0.0023 0.0001 0.003 0.001 0.0004 0.0003 0.0014 0 0.0007 0.003 0.0036 0.0013 0.0005 0.0009 0.0015 0.0031 0 0.0005
Bud Site 0.004 0.0012 0.0021 0.0009 0.0009 0.0001 0.0147 0.0001 0.0014 0.0081 0.0029 0.0002 0.0008 0.0064 0.0096 0.006 0 0.0001
Cell Periphery 0.0002 0 0.0001 0.0001 0.0001 0 0.0007 0 0.0001 0.0008 0.0002 0.0001 0.0001 0.0001 0.0002 0.0005 0 0
Cytoplasm 0.8713 0.9803 0.9239 0.7736 0.7622 0.9438 0.7793 0.9936 0.9142 0.802 0.6889 0.9311 0.8625 0.9651 0.8983 0.791 0.9123 0.9502
Cytoplasmic Foci 0.0207 0.003 0.0055 0.024 0.0111 0.0055 0.0202 0.0006 0.0098 0.01 0.0239 0.0024 0.0149 0.0021 0.0127 0.0336 0.0022 0.0084
Eisosomes 0.0001 0 0 0.0003 0.0005 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0.0003 0 0
Endoplasmic Reticulum 0.0036 0.0006 0.0035 0.0047 0.0066 0.0036 0.011 0.0002 0.002 0.006 0.0028 0.0053 0.0033 0.0007 0.0029 0.0016 0.0006 0.0022
Endosome 0.011 0.0011 0.0055 0.0209 0.03 0.0043 0.0378 0.0001 0.0178 0.0567 0.1227 0.0128 0.0187 0.0004 0.015 0.0215 0.0009 0.0108
Golgi 0.0034 0.0008 0.0009 0.0121 0.0138 0.0005 0.0094 0 0.0062 0.0251 0.0796 0.0048 0.0053 0.0001 0.0057 0.0199 0.0001 0.0027
Lipid Particles 0.0106 0 0.0011 0.0094 0.0503 0.0008 0.0093 0 0.0036 0.0031 0.0066 0.0007 0.008 0 0.0064 0.0113 0 0.0023
Mitochondria 0.0042 0.0002 0.0008 0.0032 0.0241 0.0006 0.0066 0 0.0129 0.018 0.0158 0.0044 0.0024 0.0001 0.0028 0.038 0.0767 0.0004
None 0.0074 0.0017 0.0091 0.0023 0.0021 0.0018 0.0054 0.0007 0.0019 0.0016 0.0018 0.001 0.0154 0.011 0.0055 0.0022 0.0015 0.0037
Nuclear Periphery 0.0037 0.0009 0.0045 0.0065 0.0092 0.0077 0.0112 0.0004 0.002 0.0047 0.002 0.0083 0.0098 0.0012 0.0016 0.0015 0.0006 0.0026
Nucleolus 0.0002 0 0.0002 0.0001 0.0011 0.0001 0.001 0 0.0001 0.0003 0.0001 0.0001 0.0004 0 0.0001 0.0002 0 0
Nucleus 0.0122 0.0051 0.02 0.0422 0.0179 0.0218 0.0137 0.0037 0.0076 0.0215 0.0033 0.0116 0.0199 0.0074 0.0138 0.0192 0.0035 0.0109
Peroxisomes 0.006 0.0003 0.0018 0.0398 0.0167 0.0002 0.0059 0 0.0029 0.0009 0.0028 0.0002 0.0079 0 0.0035 0.0113 0.0001 0.0009
Punctate Nuclear 0.0086 0.0006 0.0028 0.0057 0.0072 0.0028 0.0104 0.0001 0.0011 0.0008 0.0008 0.0008 0.0022 0.0005 0.001 0.0012 0.0002 0.0009
Vacuole 0.0012 0.0004 0.0011 0.001 0.0036 0.0007 0.0053 0.0001 0.0029 0.0132 0.0035 0.0009 0.0031 0.0008 0.0012 0.0018 0.0002 0.0008
Vacuole Periphery 0.0004 0.0001 0.0002 0.0009 0.0016 0.0004 0.0018 0 0.0011 0.0038 0.0027 0.0011 0.0017 0.0001 0.0003 0.0007 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.6971 50.1259 59.8912 58.7017 53.6017 46.5434 66.1447 57.3984 80.1999 55.7824
Translational Efficiency 3.4591 3.5174 2.5956 2.9207 2.7857 3.8826 2.4267 2.4877 1.8508 2.2906

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (86%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sfa1

Sfa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sfa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available