Standard name | |
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Human Ortholog | |
Description | NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 2 | 5 | 5 | 20 | 17 | 33 | 30 | – | 29 | 46 | 3 | 14 | 24 | 4 | 0 | 0 | – | 0 | 2 | 4 | 15 | 10 |
Bud | – | – | 20 | 8 | 5 | 12 | 12 | 11 | 6 | – | 10 | 18 | 8 | 5 | 34 | 6 | 1 | 4 | – | 0 | 7 | 12 | 16 | 36 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 8 | 14 |
Bud Site | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 12 | 9 | 25 | 23 | 35 | 20 | 14 | – | 27 | 37 | 14 | 9 | 12 | 3 | 10 | 2 | – | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | – | – | 367 | 280 | 357 | 392 | 385 | 379 | 245 | – | 188 | 211 | 409 | 383 | 474 | 351 | 268 | 348 | – | 0 | 453 | 421 | 285 | 307 |
Endoplasmic Reticulum | – | – | 39 | 31 | 65 | 86 | 105 | 110 | 89 | – | 41 | 92 | 31 | 29 | 27 | 30 | 17 | 24 | – | 0 | 39 | 40 | 57 | 63 |
Endosome | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | – | 0 | 2 | 7 | 16 | 19 |
Golgi | – | – | 6 | 7 | 11 | 8 | 44 | 50 | 25 | – | 30 | 47 | 25 | 47 | 23 | 92 | 85 | 57 | – | 0 | 6 | 13 | 41 | 23 |
Mitochondria | – | – | 1 | 4 | 0 | 8 | 8 | 6 | 36 | – | 43 | 44 | 23 | 6 | 8 | 17 | 18 | 17 | – | 0 | 2 | 1 | 2 | 6 |
Nucleus | – | – | 0 | 3 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 6 | 2 | 3 | – | 0 | 1 | 2 | 0 | 0 |
Nuclear Periphery | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 1 | 2 |
Nucleolus | – | – | 4 | 3 | 2 | 3 | 4 | 4 | 2 | – | 2 | 9 | 115 | 53 | 17 | 49 | 46 | 42 | – | 0 | 0 | 3 | 14 | 30 |
Peroxisomes | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | – | 0 | 2 | 4 | 15 | 20 |
SpindlePole | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 7 | 2 | 2 | 2 | 3 | – | 0 | 3 | 12 | 42 | 82 |
Vac/Vac Membrane | – | – | 22 | 0 | 2 | 2 | 8 | 25 | 3 | – | 7 | 30 | 11 | 10 | 29 | 97 | 43 | 66 | – | 0 | 24 | 14 | 15 | 16 |
Unique Cell Count | 441 | 326 | 440 | 505 | 556 | 592 | 388 | 316 | 433 | 591 | 526 | 592 | 585 | 434 | 519 | 1 | 555 | 555 | 548 | 654 | ||||
Labelled Cell Count | 473 | 350 | 472 | 554 | 618 | 639 | 450 | 377 | 534 | 642 | 565 | 655 | 657 | 493 | 568 | 1 | 555 | 555 | 548 | 654 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 50.2 | 49.8 | 53.3 | 49.8 | 50.0 | 47.4 | 47.0 | – | 46.0 | 45.3 | 53.3 | 41.8 | 32.7 | 64.8 | 74.1 | 66.4 | 57.9 | 63.2 | 62.9 |
Std Deviation (1e-4) | – | – | 12.6 | 11.8 | 12.7 | 13.2 | 12.7 | 12.7 | 14.2 | – | 15.0 | 14.4 | 11.8 | 9.9 | 8.1 | 24.7 | 26.1 | 24.9 | 14.6 | 17.2 | 16.8 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.09 | -0.01 | -0.01 | -0.08 | -0.1 | – | -0.13 | -0.15 | 0.09 | -0.26 | -0.62 | 0.37 | 0.56 | 0.4 | 0.21 | 0.33 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 3.6 | 3.0 | 4.5 | 5.4 | – | 6.0 | 6.6 | 0 | 2.7 | 3.7 | 0 | 0 | 0 |
Bud | -1.5 | -3.0 | -1.8 | -2.1 | -2.5 | -2.5 | – | -1.0 | -0.3 | -3.1 | -3.5 | 0.9 | -3.5 | -4.2 | -3.7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 2.2 | 1.5 | 2.6 | 0.6 | 0.7 | – | 3.6 | 3.7 | -0.4 | -1.1 | -0.7 | -2.9 | -0.4 | -3.0 |
Cytoplasm | 1.0 | -0.8 | -2.2 | -5.1 | -6.8 | -6.6 | – | -7.3 | -10.8 | -5.2 | -3.9 | -1.3 | -8.0 | -7.1 | -5.7 |
Endoplasmic Reticulum | 0.3 | 2.7 | 3.7 | 4.5 | 4.4 | 5.6 | – | 1.8 | 5.1 | -2.3 | -2.0 | -2.8 | -2.4 | -3.0 | -2.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.8 | 1.2 | 0.3 | 4.7 | 5.0 | 3.8 | – | 5.2 | 5.9 | 2.7 | 5.2 | 2.4 | 7.8 | 8.8 | 6.0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 6.3 | – | 7.8 | 6.6 | 3.9 | 0 | 0 | 3.2 | 4.0 | 3.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1.4 | 9.2 | 6.0 | 2.2 | 5.4 | 6.2 | 5.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.1 | -4.1 | -4.5 | -3.3 | -0.6 | -3.5 | – | -2.0 | 1.2 | -2.8 | -2.7 | -0.1 | 5.7 | 2.8 | 4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 36.0082 | 75.3924 | 67.5519 | 49.1101 | 55.9378 | 53.1203 | 37.5103 | 79.9334 | 70.6491 | 63.3838 | 58.9011 | 67.3954 | 49.3138 | 94.9088 | 75.9952 | 71.0519 | 69.2625 | 73.3492 |
Actin | 0.0232 | 0.001 | 0.0016 | 0.0044 | 0.001 | 0.0056 | 0.0099 | 0.0015 | 0.0011 | 0.0052 | 0.0132 | 0.0024 | 0.0152 | 0.0016 | 0.0023 | 0.0001 | 0.0053 | 0.0014 |
Bud | 0.0094 | 0.0128 | 0.0029 | 0.0375 | 0.0008 | 0.0015 | 0.0238 | 0.0159 | 0.0112 | 0.0116 | 0.0328 | 0.0155 | 0.0115 | 0.0151 | 0.0086 | 0.0046 | 0.0118 | 0.01 |
Bud Neck | 0.0023 | 0.0113 | 0.0113 | 0.0018 | 0.0007 | 0.0034 | 0.0012 | 0.0077 | 0.0046 | 0.0046 | 0.0189 | 0.0495 | 0.002 | 0.0086 | 0.0215 | 0.005 | 0.0385 | 0.0208 |
Bud Periphery | 0.0076 | 0.0034 | 0.001 | 0.0094 | 0.0007 | 0.0017 | 0.0058 | 0.0035 | 0.0028 | 0.0028 | 0.0094 | 0.0081 | 0.0036 | 0.0037 | 0.0029 | 0.0014 | 0.0085 | 0.0025 |
Bud Site | 0.0386 | 0.0409 | 0.0144 | 0.0637 | 0.0029 | 0.0016 | 0.0294 | 0.0438 | 0.0223 | 0.0365 | 0.0143 | 0.0059 | 0.0271 | 0.0907 | 0.026 | 0.0108 | 0.031 | 0.0063 |
Cell Periphery | 0.0058 | 0.0109 | 0.0049 | 0.004 | 0.0007 | 0.0026 | 0.0122 | 0.0126 | 0.0076 | 0.012 | 0.0051 | 0.0077 | 0.0084 | 0.0085 | 0.0065 | 0.0023 | 0.0046 | 0.0045 |
Cytoplasm | 0.212 | 0.4693 | 0.4628 | 0.2373 | 0.3594 | 0.2923 | 0.3892 | 0.3933 | 0.3719 | 0.4213 | 0.2656 | 0.4968 | 0.3351 | 0.383 | 0.368 | 0.4322 | 0.2669 | 0.3153 |
Cytoplasmic Foci | 0.2071 | 0.098 | 0.1389 | 0.2842 | 0.1185 | 0.155 | 0.1749 | 0.2198 | 0.1879 | 0.1873 | 0.4236 | 0.0947 | 0.2863 | 0.2498 | 0.2858 | 0.283 | 0.3235 | 0.3818 |
Eisosomes | 0.0009 | 0.0006 | 0.001 | 0.0004 | 0.0002 | 0.0004 | 0.0015 | 0.0007 | 0.0004 | 0 | 0.0006 | 0.0003 | 0.0007 | 0.0004 | 0.0004 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0193 | 0.0024 | 0.0051 | 0.0017 | 0.0024 | 0.0081 | 0.0149 | 0.0053 | 0.0024 | 0.0106 | 0.0033 | 0.0092 | 0.0093 | 0.0006 | 0.0067 | 0.0011 | 0.0009 | 0.0044 |
Endosome | 0.0285 | 0.0045 | 0.012 | 0.0126 | 0.0119 | 0.0177 | 0.0255 | 0.0022 | 0.0093 | 0.0116 | 0.0194 | 0.0225 | 0.0201 | 0.0049 | 0.0079 | 0.0144 | 0.0209 | 0.0232 |
Golgi | 0.0043 | 0.0008 | 0.0015 | 0.0135 | 0.0017 | 0.0038 | 0.0042 | 0.0008 | 0.0017 | 0.0008 | 0.0075 | 0.0229 | 0.0075 | 0.002 | 0.0023 | 0.0007 | 0.0024 | 0.0096 |
Lipid Particles | 0.0331 | 0.0068 | 0.01 | 0.0178 | 0.0519 | 0.0361 | 0.0192 | 0.0106 | 0.0106 | 0.0134 | 0.01 | 0.0051 | 0.0249 | 0.0104 | 0.011 | 0.0153 | 0.0319 | 0.0254 |
Mitochondria | 0.1225 | 0.0832 | 0.084 | 0.2121 | 0.2246 | 0.1555 | 0.0455 | 0.0565 | 0.0758 | 0.0089 | 0.0389 | 0.035 | 0.0401 | 0.0398 | 0.0393 | 0.0284 | 0.0671 | 0.0505 |
None | 0.0043 | 0.0038 | 0.001 | 0.0087 | 0.0156 | 0.0017 | 0.0161 | 0.0067 | 0.008 | 0.0054 | 0.0093 | 0.0071 | 0.0081 | 0.0039 | 0.0041 | 0.0005 | 0.0025 | 0.0025 |
Nuclear Periphery | 0.067 | 0.0036 | 0.0082 | 0.0074 | 0.0054 | 0.0296 | 0.0418 | 0.0017 | 0.0035 | 0.0077 | 0.0129 | 0.002 | 0.0082 | 0.0004 | 0.0022 | 0.0015 | 0.0022 | 0.0022 |
Nucleolus | 0.0561 | 0.141 | 0.1262 | 0.0259 | 0.0143 | 0.1664 | 0.0674 | 0.1389 | 0.1907 | 0.0922 | 0.0179 | 0.1079 | 0.0942 | 0.1088 | 0.124 | 0.141 | 0.0893 | 0.0707 |
Nucleus | 0.0244 | 0.0118 | 0.0084 | 0.0072 | 0.0057 | 0.0352 | 0.0516 | 0.0174 | 0.0149 | 0.0064 | 0.0088 | 0.0131 | 0.0103 | 0.0118 | 0.0127 | 0.0068 | 0.0062 | 0.0094 |
Peroxisomes | 0.0251 | 0.0077 | 0.0133 | 0.0049 | 0.0773 | 0.0201 | 0.0061 | 0.0081 | 0.017 | 0.012 | 0.0037 | 0.0059 | 0.0089 | 0.011 | 0.0145 | 0.0092 | 0.0264 | 0.0165 |
Punctate Nuclear | 0.0021 | 0.0006 | 0.001 | 0.0018 | 0.0027 | 0.0079 | 0.0056 | 0.0046 | 0.0011 | 0.0017 | 0.0017 | 0.0008 | 0.0034 | 0.0009 | 0.0018 | 0.0024 | 0.0131 | 0.0018 |
Vacuole | 0.0697 | 0.0697 | 0.0629 | 0.0341 | 0.0564 | 0.0235 | 0.0395 | 0.0392 | 0.0445 | 0.1172 | 0.0674 | 0.0604 | 0.0618 | 0.0381 | 0.0413 | 0.0325 | 0.0273 | 0.0331 |
Vacuole Periphery | 0.0367 | 0.016 | 0.0276 | 0.0096 | 0.0454 | 0.0305 | 0.0145 | 0.0091 | 0.0108 | 0.0307 | 0.0157 | 0.0272 | 0.0132 | 0.0059 | 0.0102 | 0.0068 | 0.0195 | 0.0079 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 156.9069 | 154.8969 | 154.1631 | 168.2597 | 169.2158 | 117.508 | 141.7445 | 130.2235 | 121.1336 | 129.6625 |
Translational Efficiency | 0.6568 | 0.6885 | 0.6367 | 0.5978 | 0.5705 | 0.6086 | 0.6014 | 0.4431 | 0.465 | 0.444 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1842 | 1255 | 1721 | 1072 | 1347 | 1395 | 2148 | 315 | 3189 | 2650 | 3869 | 1387 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2480.61 | 4202.59 | 3652.99 | 2052.39 | 2860.51 | 3737.67 | 2743.40 | 2944.42 | 2641.08 | 3957.85 | 3148.00 | 2254.98 |
Standard Deviation | 496.69 | 1004.02 | 872.42 | 479.93 | 983.70 | 872.38 | 579.68 | 623.58 | 765.79 | 965.36 | 854.07 | 637.18 |
Intensity Change Log 2 | — | 0.760584 | 0.558383 | -0.273390 | — | 0.385867 | -0.060307 | 0.041711 | — | 0.572044 | 0.260116 | -0.096135 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000200 | 0.000224 | 0.000452 | 0.000448 | 0.000273 | 0.000135 | 0.000270 | 0.000039 | 0.000231 | 0.000177 | 0.000351 | 0.000355 |
Bud Neck | 0.001362 | 0.013906 | 0.051653 | 0.003478 | 0.013179 | 0.005509 | 0.011644 | 0.011586 | 0.006353 | 0.009486 | 0.029441 | 0.005320 |
Bud Site | 0.040583 | 0.087289 | 0.178982* | 0.038211 | 0.093749 | 0.046007 | 0.057186 | 0.107505* | 0.063039 | 0.065558 | 0.111363* | 0.053949 |
Cell Periphery | 0.003964 | 0.004828 | 0.001496 | 0.003326 | 0.006436 | 0.004792 | 0.001012 | 0.000490 | 0.005008 | 0.004809 | 0.001227 | 0.002682 |
Cytoplasm | 0.641926* | 0.262238* | 0.091916 | 0.525517* | 0.400272* | 0.495586* | 0.375198* | 0.541852* | 0.539854* | 0.385076* | 0.249189* | 0.529227* |
Cytoplasmic Foci | 0.088107 | 0.231871* | 0.456280* | 0.055279 | 0.178598 | 0.118354 | 0.099370 | 0.086230 | 0.126329 | 0.172114 | 0.258130* | 0.062308 |
Eisosomes | 0.000003 | 0.000032 | 0.000296 | 0.000008 | 0.000099 | 0.000010 | 0.000035 | 0.000006 | 0.000044 | 0.000020 | 0.000151 | 0.000008 |
Endoplasmic Reticulum | 0.002542 | 0.001066 | 0.000307 | 0.001038 | 0.002046 | 0.001815 | 0.001203 | 0.000181 | 0.002332 | 0.001460 | 0.000804 | 0.000844 |
Endosome | 0.004461 | 0.006237 | 0.010200 | 0.015125 | 0.005334 | 0.003631 | 0.009721 | 0.012965 | 0.004830 | 0.004865 | 0.009934 | 0.014634 |
Golgi | 0.083392 | 0.178484* | 0.026646 | 0.058759 | 0.105464 | 0.153209* | 0.032028 | 0.022614 | 0.092715 | 0.165179* | 0.029634 | 0.050550 |
Lipid Particles | 0.005597 | 0.009041 | 0.003002 | 0.012225 | 0.008793 | 0.008288 | 0.003527 | 0.000240 | 0.006947 | 0.008645 | 0.003294 | 0.009503 |
Mitochondria | 0.001222 | 0.000628 | 0.010194 | 0.000943 | 0.000876 | 0.000557 | 0.000952 | 0.003383 | 0.001076 | 0.000591 | 0.005063 | 0.001497 |
Mitotic Spindle | 0.002808 | 0.006184 | 0.036405 | 0.019048 | 0.002319 | 0.003893 | 0.022642 | 0.006884 | 0.002601 | 0.004978 | 0.028764 | 0.016286 |
None | 0.001716 | 0.002469 | 0.000829 | 0.000965 | 0.008903 | 0.001714 | 0.001066 | 0.000669 | 0.004751 | 0.002071 | 0.000961 | 0.000898 |
Nuclear Periphery | 0.000112 | 0.000495 | 0.000911 | 0.000311 | 0.000252 | 0.000288 | 0.001027 | 0.000036 | 0.000171 | 0.000386 | 0.000976 | 0.000248 |
Nuclear Periphery Foci | 0.004149 | 0.013323 | 0.007140 | 0.006480 | 0.012275 | 0.012334 | 0.013330 | 0.000201 | 0.007581 | 0.012802 | 0.010577 | 0.005054 |
Nucleolus | 0.000087 | 0.001312 | 0.008472 | 0.001056 | 0.003187 | 0.000614 | 0.002033 | 0.000364 | 0.001396 | 0.000944 | 0.004897 | 0.000899 |
Nucleus | 0.001788 | 0.004845 | 0.006069 | 0.006637 | 0.008743 | 0.002668 | 0.010665 | 0.002143 | 0.004726 | 0.003699 | 0.008620 | 0.005616 |
Peroxisomes | 0.000285 | 0.002498 | 0.022156 | 0.001059 | 0.004770 | 0.000785 | 0.001566 | 0.001500 | 0.002180 | 0.001596 | 0.010725 | 0.001159 |
Vacuole | 0.022049 | 0.070676 | 0.062099 | 0.204561* | 0.064596 | 0.043003 | 0.262765* | 0.133967 | 0.040020 | 0.056108 | 0.173505 | 0.188529 |
Vacuole Periphery | 0.093649 | 0.102353* | 0.024496 | 0.045525 | 0.079837 | 0.096809 | 0.092759 | 0.067144 | 0.087815 | 0.099434 | 0.062394 | 0.050435 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.40 | -5.20 | -3.45 | -2.94 | 0.71 | 4.89 | -0.28 | 10.24 | 5.09 | 3.47 | 2.27 | -3.20 | -2.25 | -3.11 | 0.49 |
Bud Neck | -9.09 | -23.62 | -7.18 | 7.08 | 22.09* | 4.47 | 0.56 | 0.53 | -2.64 | 0.19 | -3.36 | -18.61 | 0.67 | 3.99 | 19.38 |
Bud Site | -8.40 | -23.81* | 0.78 | 8.81 | 24.02* | 7.46 | 6.31 | -1.17 | -5.36 | -4.58 | -0.69 | -11.55 | 2.21 | 2.71 | 12.47 |
Cell Periphery | -1.85 | 6.94 | 1.71 | 3.16 | -2.87 | 2.97 | 12.40 | 13.48 | 11.08 | 3.46 | 0.59 | 13.90 | 5.87 | 5.41 | -3.10 |
Cytoplasm | 32.09 | 59.84 | 8.93 | -18.98 | -37.06 | -6.89 | 1.88 | -6.49 | -2.16 | -7.89 | 16.30 | 35.17 | 1.08 | -12.28 | -26.02 |
Cytoplasmic Foci | -15.41 | -40.57 | 6.59 | 19.21 | 45.12* | 6.19 | 8.80 | 7.80 | 2.88 | 1.26 | -7.19 | -19.82 | 12.45 | 17.85 | 31.44* |
Eisosomes | -5.07 | -6.48 | -3.46 | 4.11 | 6.36 | 5.26 | 3.48 | 5.44 | 1.12 | 3.13 | 3.00 | -4.75 | 4.94 | 4.06 | 6.88 |
Endoplasmic Reticulum | 3.94 | 6.67 | 4.37 | 0.23 | -5.33 | 0.66 | 2.93 | 7.00 | 7.76 | 10.01 | 3.35 | 6.62 | 6.30 | 3.79 | -0.01 |
Endosome | -2.76 | -8.50 | -8.69 | -6.93 | -3.58 | 2.70 | -6.43 | -3.96 | -5.07 | -1.48 | -0.10 | -10.91 | -9.39 | -9.53 | -4.06 |
Golgi | -12.56 | 15.12 | 6.60 | 16.98 | -7.01 | -6.75 | 15.03 | 13.23 | 19.65 | 1.74 | -14.14 | 21.27 | 11.96 | 23.75 | -5.88 |
Lipid Particles | -3.04 | 2.76 | -5.01 | -2.23 | -7.52 | 0.37 | 5.22 | 9.82 | 9.43 | 11.76 | -2.09 | 5.39 | -2.52 | -0.83 | -6.46 |
Mitochondria | 3.16 | -5.85 | 1.53 | -0.72 | 6.09 | 1.63 | -0.42 | -4.72 | -5.70 | -4.67 | 3.64 | -5.73 | -1.41 | -3.79 | 5.08 |
Mitotic Spindle | -5.34 | -13.95 | -6.11 | -4.81 | 5.02 | -2.50 | -12.10 | -1.92 | -1.37 | 5.32 | -5.48 | -18.40 | -6.43 | -5.34 | 5.19 |
None | -1.20 | 7.56 | 2.48 | 2.24 | 0.01 | 6.64 | 7.24 | 7.54 | 4.84 | 2.02 | 4.80 | 8.07 | 7.42 | 3.07 | 0.59 |
Nuclear Periphery | -10.37 | -8.29 | -4.68 | 2.61 | 5.81 | -1.32 | -9.34 | 11.54 | 10.04 | 11.74 | -9.51 | -12.73 | -2.73 | 2.62 | 10.06 |
Nuclear Periphery Foci | -8.87 | -4.27 | -3.85 | 5.68 | 1.00 | -0.12 | -1.07 | 12.70 | 11.05 | 13.44 | -6.06 | -4.39 | 3.54 | 8.60 | 7.17 |
Nucleolus | -3.16 | -8.66 | -1.91 | 0.76 | 7.02 | 3.89 | 1.67 | 4.90 | 0.60 | 3.91 | 1.05 | -6.49 | 1.32 | 0.41 | 6.76 |
Nucleus | -6.80 | -10.03 | -5.67 | -2.20 | -0.41 | 7.25 | -3.41 | 7.20 | 0.90 | 10.57 | 1.64 | -8.80 | -2.18 | -3.03 | 3.64 |
Peroxisomes | -5.49 | -15.96 | -1.94 | 2.87 | 14.87 | 4.43 | 3.64 | 3.57 | -2.12 | -0.04 | 1.34 | -11.14 | 2.33 | 1.39 | 13.54 |
Vacuole | -14.04 | -12.18 | -25.08 | -17.21 | -17.88 | 5.82 | -29.98 | -5.58 | -7.57 | 10.11 | -6.70 | -32.29 | -23.35 | -20.53 | -2.91 |
Vacuole Periphery | -1.07 | 17.07 | 11.98 | 11.72 | -5.59 | -3.27 | -0.92 | 1.76 | 4.02 | 2.45 | -2.67 | 9.49 | 11.49 | 13.74 | 3.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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