Standard name
Human Ortholog
Description Anti-silencing protein; causes derepression of silent loci when overexpressed
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.36 0.54 0.52 0.42 0.38 0.35 0.34 0.4 0.35 0.28 0.44 0.42 0.43 0.53 0.29 0.16 0.25 0.18 0.25 0.23 0.41 0.33 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.1 0 0 0 0 0 0 0 0.1 0.15 0.24 0.34 0.25 0.28 0.23 0.12 0.12 0.1 0.14 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.64 0.63 0.42 0.43 0.35 0.63 0.67 0.66 0.69 0.73 0.72 0.64 0.16 0.15 0.06 0.17 0.21 0.12 0.16 0.15 0.18 0.14 0.17 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.09 0 0.14 0.13 0.19 0 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.05 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.13 0.05 0.07 0.06 0 0 0 0 0.05 0.05 0.05 0.12 0.08 0.09 0.17 0.2 0.13 0.2 0.17 0.1
Vac/Vac Membrane 0.12 0.22 0.05 0.06 0.1 0 0 0 0 0 0 0 0.2 0.25 0.21 0.21 0.4 0.19 0.07 0.09 0.11 0.06 0 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 1 2 5
Bud 0 4 4 3 2 9 7 15 8 7 2 6 0 0 1 0 0 0 1 3 0 0 2 4
Bud Neck 0 1 1 1 5 2 2 10 3 1 2 0 0 0 0 0 0 0 1 1 1 0 2 2
Bud Site 0 0 1 1 0 5 0 2 6 0 3 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 82 64 64 71 91 111 141 123 124 95 33 85 51 115 121 42 10 8 57 55 34 56 73 74
Endoplasmic Reticulum 3 1 4 1 3 0 0 2 0 0 3 1 0 3 4 10 4 1 6 1 1 2 5 1
Endosome 6 4 1 3 21 4 2 1 4 1 0 0 12 39 55 50 16 9 72 27 17 13 30 12
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 2 3
Mitochondria 156 112 50 58 76 185 274 241 216 197 84 125 19 40 14 25 13 4 49 33 26 19 37 26
Nucleus 2 5 4 3 2 6 9 9 11 5 1 6 22 24 9 20 8 6 5 10 6 1 1 1
Nuclear Periphery 3 4 0 1 0 0 2 3 0 1 0 0 0 2 0 1 0 0 11 5 4 0 1 0
Nucleolus 2 0 0 0 0 1 2 0 5 0 0 1 9 4 3 6 3 2 2 3 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 0 0 0 1 0 0 0 0 0
SpindlePole 5 3 3 10 27 15 27 21 14 3 0 6 6 12 12 17 5 3 51 43 19 27 37 18
Vac/Vac Membrane 30 39 6 8 22 5 9 15 6 12 1 6 24 66 48 31 25 6 21 20 16 8 9 20
Unique Cell Count 244 177 119 136 215 293 407 367 312 269 116 195 122 265 228 147 63 32 309 221 149 139 219 181
Labelled Cell Count 290 237 138 160 249 343 475 444 398 322 129 240 145 307 269 203 84 39 309 221 149 139 219 181


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.1 3.3 3.4 3.3 3.0 2.6 2.9 2.8 2.5 2.6 2.6 4.4 4.1 4.2 5.1 5.2 5.8 4.4 4.3 4.8
Std Deviation (1e-4) 0.5 0.6 0.8 1.0 1.0 1.2 0.8 0.8 1.3 0.8 0.7 1.2 1.0 1.0 1.2 1.8 1.2 0.8 2.2 1.8 1.7
Intensity Change (Log2) – – – 0.04 0.03 -0.14 -0.33 -0.17 -0.23 -0.41 -0.31 -0.35 0.42 0.33 0.35 0.64 0.65 0.82 0.44 0.38 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.8 5.9 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2061 2.6226 2.4179 2.2133 1.4821 2.1055 -0.2476 0.2246 0.2212 -0.4448 -0.308 -0.2733 1.2405 1.4102 1.274 0.867 1.227 0.8977
Actin 0.0366 0.002 0.0124 0.0064 0.0012 0.0018 0.0214 0.0013 0.0382 0.0034 0.0017 0.0029 0.0742 0.0022 0.0269 0.0035 0.0215 0.0051
Bud 0.0005 0.0002 0.0002 0.0004 0.0001 0.0001 0.0005 0.0001 0.0006 0.0115 0 0.0001 0.0007 0.0001 0.0002 0.0001 0.0002 0.0002
Bud Neck 0.0022 0.0054 0.0028 0.012 0.0083 0.0087 0.0011 0.0007 0.0019 0.0013 0.0009 0.0038 0.0022 0.0008 0.0015 0.0011 0.004 0.0069
Bud Periphery 0.0007 0.0003 0.0003 0.0006 0.0001 0.0002 0.0008 0.0001 0.0008 0.0036 0 0.0002 0.0014 0.0001 0.0002 0.0001 0.0005 0.0006
Bud Site 0.0097 0.0075 0.0034 0.0074 0.0003 0.0007 0.0015 0.0017 0.0117 0.0017 0.0001 0.0003 0.0035 0.0024 0.002 0.0005 0.0006 0.0007
Cell Periphery 0.0003 0.0034 0.0002 0.0005 0.0001 0.0003 0.0003 0.0002 0.0004 0.001 0.0001 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003
Cytoplasm 0.0826 0.0956 0.0732 0.0262 0.0546 0.0865 0.1027 0.1173 0.0535 0.0725 0.0896 0.076 0.0745 0.0909 0.0752 0.089 0.1 0.1063
Cytoplasmic Foci 0.0689 0.0248 0.0294 0.0431 0.0455 0.0408 0.0584 0.0183 0.0396 0.0338 0.0235 0.0532 0.0811 0.1029 0.0282 0.0416 0.0464 0.036
Eisosomes 0.0006 0.0002 0.0002 0.0001 0.0002 0.0002 0.0005 0.0002 0.0013 0.0004 0.0003 0.0002 0.001 0.0001 0.0002 0.0001 0.0004 0.0002
Endoplasmic Reticulum 0.0097 0.0175 0.0119 0.0056 0.0062 0.0081 0.0181 0.0198 0.0121 0.0086 0.0193 0.0185 0.0142 0.0173 0.0145 0.0122 0.0139 0.0115
Endosome 0.0286 0.0262 0.0211 0.0335 0.0284 0.0242 0.0363 0.0276 0.0511 0.0119 0.0343 0.0448 0.0518 0.0464 0.0317 0.0462 0.062 0.0256
Golgi 0.0054 0.0017 0.0031 0.0061 0.0016 0.0015 0.0047 0.002 0.0084 0.0022 0.0007 0.0025 0.0105 0.0034 0.0041 0.0037 0.0066 0.0035
Lipid Particles 0.101 0.0252 0.0479 0.0159 0.0365 0.0211 0.0177 0.012 0.0394 0.036 0.0122 0.0495 0.0451 0.0407 0.0332 0.0535 0.0683 0.0309
Mitochondria 0.0039 0.0008 0.0011 0.0026 0.0012 0.0012 0.0046 0.0321 0.0235 0.0056 0.0006 0.0025 0.0053 0.0011 0.0016 0.0017 0.0048 0.0042
None 0.2329 0.228 0.1702 0.0814 0.1943 0.1262 0.3659 0.3263 0.1524 0.162 0.4875 0.2209 0.212 0.1516 0.1437 0.0982 0.151 0.1398
Nuclear Periphery 0.1291 0.1249 0.1413 0.1409 0.0991 0.0955 0.0874 0.1176 0.2174 0.0708 0.105 0.1291 0.1056 0.126 0.1936 0.1268 0.1478 0.0881
Nucleolus 0.0093 0.0195 0.0184 0.0556 0.0141 0.0307 0.0125 0.0191 0.0174 0.0148 0.0031 0.0287 0.0071 0.0089 0.0232 0.0103 0.0167 0.0251
Nucleus 0.0435 0.1878 0.1656 0.2389 0.1442 0.2358 0.0834 0.1402 0.1325 0.1903 0.0835 0.1393 0.0741 0.1018 0.1596 0.234 0.0714 0.1574
Peroxisomes 0.0191 0.0026 0.0068 0.0039 0.0047 0.0031 0.0016 0.0009 0.0084 0.0427 0.001 0.0031 0.0187 0.0057 0.0116 0.0041 0.0114 0.0039
Punctate Nuclear 0.2092 0.219 0.2857 0.3108 0.3558 0.3081 0.1742 0.1544 0.1817 0.3214 0.1252 0.2119 0.2091 0.2916 0.2422 0.2662 0.2664 0.3467
Vacuole 0.0045 0.0059 0.003 0.0045 0.0022 0.0038 0.0041 0.0059 0.0037 0.003 0.0098 0.0085 0.0043 0.0038 0.0035 0.0041 0.0029 0.0046
Vacuole Periphery 0.002 0.0016 0.0019 0.0036 0.0014 0.0016 0.0023 0.0022 0.004 0.0018 0.0013 0.0038 0.0034 0.0021 0.0029 0.0028 0.0031 0.0022

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.1239 6.8759 5.1022 9.3917 24.0249 28.6531 13.5884 8.7447 10.7993 19.5913
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1082 1085 1497 1543 1040 1844 209 180 2122 2929 1706 1723

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.69 678.48 824.72 796.13 669.09 707.76 963.05 867.75 657.16 696.91 841.67 803.61
Standard Deviation 62.14 98.27 102.89 115.15 77.22 85.11 103.97 100.72 70.91 91.31 112.56 115.82
Intensity Change Log 2 — 0.071465 0.353063 0.302162 — 0.081060 0.525410 0.375079 — 0.076356 0.443340 0.339730

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000021 0.000720 0.000376 0.000505 0.000105 0.000249 0.001445 0.001311 0.000062 0.000423 0.000507 0.000590
Bud Neck 0.002693 0.014703 0.004466 0.013218 0.003807 0.012508 0.003763 0.008282 0.003239 0.013321 0.004380 0.012702
Bud Site 0.000360 0.006565 0.005748 0.028595 0.000701 0.004934 0.002259 0.042330 0.000527 0.005538 0.005321 0.030030
Cell Periphery 0.000120 0.000177 0.000159 0.000195 0.000141 0.000117 0.000231 0.000198 0.000130 0.000139 0.000168 0.000196
Cytoplasm 0.444796* 0.215204* 0.185472* 0.327939* 0.303716* 0.237412* 0.095323 0.281291* 0.375652* 0.229185* 0.174428* 0.323065*
Cytoplasmic Foci 0.115923 0.181790* 0.008178 0.007146 0.221183* 0.206843* 0.006372 0.030505 0.167511 0.197562* 0.007957 0.009586
Eisosomes 0.000089 0.000143 0.000045 0.000035 0.000140 0.000119 0.000146 0.000095 0.000114 0.000128 0.000057 0.000041
Endoplasmic Reticulum 0.000545 0.007138 0.004537 0.004182 0.003119 0.003400 0.019558 0.004611 0.001807 0.004784 0.006377 0.004227
Endosome 0.002142 0.022470 0.002102 0.004494 0.011679 0.016918 0.004698 0.021658 0.006816 0.018974 0.002420 0.006287
Golgi 0.000262 0.005937 0.000100 0.000709 0.001746 0.003846 0.000192 0.007185 0.000989 0.004621 0.000111 0.001386
Lipid Particles 0.020770 0.069477 0.002988 0.001403 0.097194 0.104686* 0.006472 0.005097 0.058226 0.091643 0.003415 0.001789
Mitochondria 0.002195 0.036129 0.001341 0.002669 0.008535 0.027849 0.001189 0.005729 0.005302 0.030916 0.001322 0.002989
Mitotic Spindle 0.000471 0.070300 0.005276 0.068692 0.007546 0.059631 0.005681 0.052088 0.003939 0.063583 0.005325 0.066957
None 0.013264 0.015648 0.009778 0.013062 0.012976 0.010236 0.006341 0.013358 0.013123 0.012241 0.009357 0.013093
Nuclear Periphery 0.002670 0.011519 0.005183 0.004441 0.008880 0.015722 0.005671 0.003380 0.005713 0.014165 0.005243 0.004330
Nuclear Periphery Foci 0.002891 0.032446 0.005650 0.004090 0.024188 0.032771 0.028344 0.003554 0.013329 0.032650 0.008430 0.004034
Nucleolus 0.006797 0.026415 0.007560 0.002672 0.015819 0.024153 0.008913 0.001563 0.011219 0.024991 0.007726 0.002556
Nucleus 0.377100* 0.232729* 0.729701* 0.462167* 0.256790* 0.206026* 0.721255* 0.391708* 0.318135* 0.215917* 0.728666* 0.454807*
Peroxisomes 0.001964 0.017940 0.000529 0.000644 0.008236 0.014243 0.000966 0.000912 0.005038 0.015613 0.000583 0.000672
Vacuole 0.004741 0.028854 0.020474 0.050718 0.012681 0.016506 0.080807 0.123560 0.008632 0.021080 0.027866 0.058327
Vacuole Periphery 0.000189 0.003698 0.000338 0.002424 0.000820 0.001831 0.000374 0.001587 0.000498 0.002523 0.000343 0.002336

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.94 -9.51 -13.95 1.15 -1.07 -3.80 -9.16 -5.84 -4.85 4.29 -9.96 -11.56 -14.10 -4.39 0.77
Bud Neck -8.48 -7.36 -10.92 -0.21 -5.66 -7.55 -3.56 -2.60 1.91 -0.26 -11.31 -8.04 -11.24 -1.21 -5.63
Bud Site -6.04 -5.06 -7.89 -4.34 -4.53 -4.39 -7.07 -2.62 -2.12 -2.11 -7.23 -5.34 -8.21 -5.61 -5.01
Cell Periphery -4.51 -2.42 -2.30 1.74 0.14 4.91 -5.00 -1.69 -3.93 3.15 -0.43 -2.91 -1.86 -1.64 0.86
Cytoplasm 20.89 28.09 19.98 -2.33 -8.95 6.94 17.77 4.80 1.27 -6.35 20.06 27.98 16.47 -1.60 -10.49
Cytoplasmic Foci -5.85 22.65 23.58 29.06 3.63 2.49 35.07 30.40 33.15 -3.53 -3.24 39.62 40.51 49.78 2.06
Eisosomes -5.53 3.98 5.10 12.62 1.42 4.90 -1.50 1.19 -1.66 2.04 -1.42 8.21 11.77 14.53 2.52
Endoplasmic Reticulum -13.04 -17.11 -22.98 -2.35 -1.71 -0.32 -11.32 -6.59 -6.74 6.80 -10.25 -16.62 -18.91 -10.46 1.73
Endosome -13.74 -3.48 -2.69 13.06 0.55 -3.82 6.25 1.18 2.64 -1.09 -13.87 7.72 6.34 18.43 -0.23
Golgi -7.51 1.52 0.85 8.22 -2.47 -4.89 5.84 0.20 1.81 -1.24 -9.58 5.68 3.37 12.57 -2.12
Lipid Particles -9.79 9.02 9.93 17.24 4.03 -0.98 18.89 19.42 24.97 2.27 -7.61 20.47 21.31 30.70 4.63
Mitochondria -10.47 0.69 -0.26 10.85 -3.97 -8.58 5.20 3.65 12.64 -0.96 -14.23 4.63 3.48 17.88 -3.97
Mitotic Spindle -12.99* -6.84 -11.72 3.78 -6.87 -12.61* -1.23 -2.04 4.85 -0.79 -18.67 -4.64 -10.46 5.12 -6.84
None -0.67 3.37 3.37 3.50 -0.07 2.06 5.39 2.98 1.86 -1.25 1.11 4.93 4.61 3.90 -0.41
Nuclear Periphery -8.45 -8.66 -12.78 2.27 -1.87 -5.24 -0.52 2.32 7.49 2.54 -9.97 -4.00 -5.93 6.05 -1.29
Nuclear Periphery Foci -10.11 -5.17 -2.23 9.26 3.63 -1.97 -0.63 8.09 11.86 4.31 -8.40 0.74 6.55 15.17 5.22
Nucleolus -7.87 -2.98 4.92 11.30 8.07 -3.44 4.16 10.74 16.55 4.12 -8.23 1.33 10.65 18.91 8.75
Nucleus 12.40 -31.69 -15.76 -28.61 15.84 5.32 -18.81 -7.42 -9.67 7.45 13.83 -44.60 -24.12 -37.54 17.20
Peroxisomes -11.39 4.55 5.57 13.88 3.46 -5.40 10.41 10.82 19.20 1.75 -12.83 11.38 12.39 23.89 3.85
Vacuole -12.60 -12.43 -21.55 -14.34 -14.02 -2.14 -8.64 -11.59 -11.46 -5.24 -11.40 -13.36 -23.10 -20.13 -13.74
Vacuole Periphery -6.48 -2.62 -2.61 4.19 -2.08 -2.76 3.46 0.70 2.71 -0.84 -6.81 2.15 -1.68 3.57 -2.14
Standard name
Human Ortholog
Description Anti-silencing protein; causes derepression of silent loci when overexpressed
Localization
Cell Percentages nucleus (28%), cytoplasm (5%), mixed (36%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (0.00019)
nucleus - S/G2 (9e-05)
Subcompartmental Group N/A

Asf2

Asf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available