Standard name
Human Ortholog
Description Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0.06 0.1 0.12 0.13 0.1 0.15 0.1 0 0 0 0.06 0.05 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.09 0.08 0.15 0.43 0.32 0.38 0.42 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.95 0.94 0.95 0.93 0.89 0.88 0.9 0.86 0.78 0.8 0.84 0.77 0.88 0.85 0.84 0.78 0.81 0.78 0.94 0.93 0.92 0.78 0.71 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.13 0 0 0 0.07 0.13 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 1 0 6 0 7 8 6 0 0 0 0 0 0 2 2 1 4 7 9
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 5 0 0 0 0 0 0 0 0 2 0 1 0
Bud Site 0 0 0 0 7 19 2 9 18 13 12 17 0 0 1 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 5 6 16 17 18 20 19 20 22 18 15 33 20 36 43 12 11 6 0 2 1 18 17 27
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 2 0 0 0 2 2 3
Endosome 0 0 0 0 0 2 0 1 0 1 1 0 2 1 0 1 0 0 1 4 1 2 5 7
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 1
Mitochondria 0 8 0 0 28 39 40 73 216 168 165 232 1 3 1 7 3 2 0 2 1 2 2 7
Nucleus 180 291 392 260 278 372 453 421 387 422 365 419 181 245 281 92 61 49 184 296 398 237 272 230
Nuclear Periphery 0 0 2 1 3 1 9 4 6 3 3 1 0 0 1 2 0 0 0 0 0 0 1 3
Nucleolus 2 1 0 1 11 12 11 7 23 20 23 16 2 4 12 3 1 2 0 0 2 4 11 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 3 7 4 3 3 7 0 0 2 0 0 0 0 0 1 2 4 5
Vac/Vac Membrane 9 8 9 1 3 8 6 16 8 11 13 10 6 6 4 9 2 8 3 7 15 21 51 85
Unique Cell Count 189 308 411 279 312 421 502 488 497 527 437 546 206 289 333 118 75 63 195 318 432 305 384 408
Labelled Cell Count 196 314 419 280 349 475 543 565 684 666 608 746 213 297 345 130 79 69 195 318 432 305 384 408


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 8.1 9.8 9.2 6.3 6.2 6.0 5.5 4.5 5.0 4.6 4.4 9.8 8.6 8.9 9.1 11.6 11.0 10.5 10.7 10.1
Std Deviation (1e-4) 3.1 1.6 2.5 3.0 1.9 1.9 1.5 1.4 1.3 1.3 1.3 0.9 2.7 2.6 2.7 2.6 2.8 3.5 2.7 3.1 3.1
Intensity Change (Log2) – – – -0.08 -0.64 -0.65 -0.72 -0.83 -1.11 -0.98 -1.08 -1.15 0.0 -0.19 -0.14 -0.11 0.24 0.17 0.09 0.12 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 4.4 0 0 0 3.2 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.2 0.6 -0.1 0.2 0.4 -0.4 -0.4 1.5 2.9 4.3 4.5 2.7 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 6.2 6.3 5.9 8.2 0 12.6 0 0 0 0 0 0 0 0
Nucleus -1.2 -3.2 -3.7 -2.9 -4.6 -7.5 -6.9 -5.6 -7.9 -3.4 -4.8 -5.1 -6.0 -4.4 -5.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.4 0 0 0 4.0 0 0 0 0 3.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -1.3 -0.3 -1.2 1.0 -0.6 -0.1 0.7 -0.4 0.5 -0.1 -1.0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9984 9.2824 8.7609 8.7572 7.8255 8.2408 8.642 10.7957 10.7022 10.8595 9.3878 10.4145 5.8032 6.7449 6.1054 5.8375 6.4127 5.9371
Actin 0.085 0 0.0189 0 0.0309 0.0154 0.0294 0 0.0031 0.0001 0 0 0.0055 0 0.0019 0.0494 0 0
Bud 0.0007 0 0.0003 0 0.0002 0 0.001 0 0.0001 0 0 0 0.0002 0 0.0002 0.002 0 0
Bud Neck 0.0019 0 0.0005 0 0.0003 0.0003 0.0008 0 0.0001 0.0001 0.0001 0.0002 0.0007 0 0.0003 0.0037 0.0001 0.0013
Bud Periphery 0.0014 0 0.0004 0 0.0003 0.0001 0.0014 0 0.0002 0.0001 0 0 0.0003 0 0.0004 0.0057 0 0.0001
Bud Site 0.0043 0 0.0017 0 0.0004 0.0001 0.0028 0 0.0005 0 0 0 0.0016 0 0.0004 0.0174 0.0001 0
Cell Periphery 0.0007 0 0.0001 0 0 0 0.0004 0 0 0 0 0 0.0001 0 0.0001 0.0019 0 0.0001
Cytoplasm 0.0049 0 0.0068 0 0.0005 0.0001 0.0227 0 0 0.0001 0 0.006 0.0009 0 0.0001 0.0061 0 0
Cytoplasmic Foci 0.0114 0 0.0064 0 0.008 0.0004 0.0496 0 0.0006 0 0 0.0003 0.0123 0 0.0202 0.0088 0 0
Eisosomes 0.0023 0 0.0002 0 0.0001 0.0001 0.0004 0 0.0001 0 0 0 0 0 0 0.0019 0 0
Endoplasmic Reticulum 0.0059 0 0.0022 0 0.0008 0.0001 0.0094 0 0 0 0 0.0007 0.0006 0 0 0.0025 0 0
Endosome 0.0188 0 0.0072 0 0.0128 0.0006 0.0979 0 0.0008 0 0 0.0005 0.0099 0 0.0022 0.0159 0 0
Golgi 0.0083 0 0.006 0 0.0085 0.0008 0.0197 0 0.0009 0 0 0 0.0031 0 0.0035 0.0133 0 0
Lipid Particles 0.011 0 0.0071 0 0.0158 0.0013 0.0547 0 0.0024 0 0 0 0.0086 0 0.0034 0.0324 0 0
Mitochondria 0.0096 0.0001 0.0048 0.0001 0.0016 0.0003 0.0092 0.0002 0.0191 0.0014 0.0001 0.0001 0.0017 0.0001 0.0144 0.0191 0.0002 0
None 0.0037 0 0.0033 0 0.0009 0.0004 0.0411 0 0.0001 0 0 0.0026 0.001 0 0 0.0024 0 0
Nuclear Periphery 0.007 0.0003 0.0037 0.0002 0.004 0.0003 0.0381 0.0005 0.0005 0.0053 0.0003 0.0015 0.0101 0.0005 0.0002 0.0046 0.0006 0.0001
Nucleolus 0.0072 0.0079 0.0028 0.0044 0.0096 0.0157 0.0062 0.0053 0.0016 0.0052 0.0098 0.0079 0.006 0.0033 0.0029 0.0208 0.0267 0.0111
Nucleus 0.7947 0.9915 0.9204 0.9952 0.89 0.962 0.5553 0.9937 0.9666 0.9865 0.9895 0.978 0.9237 0.9957 0.9385 0.7441 0.9717 0.9865
Peroxisomes 0.0047 0 0.0022 0 0.0081 0.0014 0.0156 0 0.0029 0 0 0 0.0098 0 0.0069 0.0269 0 0
Punctate Nuclear 0.0101 0.0001 0.0035 0.0001 0.0069 0.0005 0.0231 0 0 0.0001 0.0001 0.0019 0.0023 0.0001 0.0032 0.003 0.0002 0.0001
Vacuole 0.005 0 0.0009 0 0.0002 0 0.0118 0 0.0002 0.0002 0 0.0001 0.0009 0.0001 0.0009 0.0136 0.0001 0.0005
Vacuole Periphery 0.0014 0 0.0004 0 0.0003 0 0.0094 0 0.0001 0.001 0 0 0.0006 0 0.0004 0.0046 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.8538 56.1352 48.4555 39.0883 58.9857 42.2674 32.1437 30.49 31.8311 44.4227
Translational Efficiency 2.1128 1.3757 1.5248 1.7204 1.2817 1.4219 1.5753 1.4006 1.3351 1.3122

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
311 729 2668 1655 1004 1541 164 1630 1315 2270 2832 3285

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 909.18 919.50 1250.84 1333.32 862.67 1089.47 1303.85 1218.26 873.67 1034.88 1253.91 1276.23
Standard Deviation 141.20 229.86 195.57 239.78 137.73 183.79 202.25 233.57 139.96 214.93 196.36 243.61
Intensity Change Log 2 — 0.016284 0.460259 0.552385 — 0.336746 0.595897 0.497941 — 0.181200 0.527892 0.526134

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000249 0.000714 0.000296 0.000531 0.000293 0.000396 0.001272 0.000691 0.000283 0.000498 0.000352 0.000610
Bud Neck 0.002238 0.008347 0.017258 0.023967 0.003090 0.009956 0.005875 0.015984 0.002888 0.009439 0.016599 0.020006
Bud Site 0.000321 0.002764 0.000621 0.002592 0.000324 0.001337 0.001368 0.002234 0.000323 0.001795 0.000665 0.002414
Cell Periphery 0.000055 0.000329 0.000132 0.000163 0.000100 0.000274 0.000073 0.000166 0.000089 0.000292 0.000129 0.000165
Cytoplasm 0.002038 0.009094 0.000641 0.002056 0.000932 0.001395 0.002617 0.003966 0.001194 0.003868 0.000755 0.003003
Cytoplasmic Foci 0.000194 0.000973 0.000010 0.000081 0.000085 0.000811 0.001486 0.000990 0.000111 0.000863 0.000095 0.000532
Eisosomes 0.000020 0.000031 0.000043 0.000033 0.000020 0.000037 0.000065 0.000042 0.000020 0.000035 0.000044 0.000038
Endoplasmic Reticulum 0.004182 0.006570 0.002428 0.002048 0.005422 0.008461 0.009966 0.002679 0.005129 0.007854 0.002864 0.002361
Endosome 0.000072 0.000461 0.000053 0.000376 0.000214 0.001296 0.000558 0.001903 0.000181 0.001027 0.000082 0.001133
Golgi 0.000021 0.000351 0.000017 0.000192 0.000033 0.001057 0.000270 0.001092 0.000030 0.000830 0.000032 0.000639
Lipid Particles 0.000065 0.000504 0.000009 0.000033 0.000132 0.000246 0.000329 0.000113 0.000116 0.000329 0.000027 0.000072
Mitochondria 0.000628 0.001167 0.000761 0.002288 0.001163 0.002842 0.001355 0.003569 0.001036 0.002304 0.000795 0.002923
Mitotic Spindle 0.000001 0.000233 0.000090 0.008894 0.000408 0.002000 0.001135 0.005896 0.000312 0.001433 0.000150 0.007406
None 0.002008 0.023683 0.000729 0.002171 0.002708 0.002741 0.000577 0.005981 0.002542 0.009466 0.000720 0.004061
Nuclear Periphery 0.000072 0.000278 0.000254 0.002101 0.000295 0.000614 0.000172 0.000712 0.000242 0.000506 0.000249 0.001412
Nuclear Periphery Foci 0.000021 0.000262 0.000012 0.000908 0.000150 0.000483 0.000269 0.000763 0.000119 0.000412 0.000027 0.000836
Nucleolus 0.003335 0.006135 0.001208 0.002551 0.006539 0.005958 0.005608 0.003999 0.005782 0.006015 0.001463 0.003269
Nucleus 0.983641* 0.935394* 0.974923* 0.946956* 0.977027* 0.955903* 0.957599* 0.946146* 0.978591* 0.949317* 0.973920* 0.946554*
Peroxisomes 0.000186 0.000278 0.000035 0.000125 0.000219 0.000285 0.006376 0.000225 0.000211 0.000283 0.000402 0.000175
Vacuole 0.000614 0.002084 0.000413 0.001351 0.000487 0.002858 0.002804 0.001880 0.000517 0.002609 0.000552 0.001614
Vacuole Periphery 0.000039 0.000346 0.000069 0.000583 0.000358 0.001049 0.000228 0.000971 0.000283 0.000823 0.000078 0.000776

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.51 -2.03 -8.96 0.85 -9.85 -3.00 -4.52 -9.82 -7.49 2.39 -5.29 -3.73 -13.22 -4.82 -9.56
Bud Neck -6.55 -16.05 -14.33 -10.10 -4.38 -7.19 -4.28 -15.88 -6.27 -9.87 -9.26 -17.55 -20.19 -11.26 -3.50
Bud Site -2.43 -3.52 -5.02 0.17 -4.24 -5.16 -1.90 -4.35 -2.36 -1.36 -4.25 -6.12 -6.59 -1.72 -5.30
Cell Periphery -4.17 -2.76 -1.50 2.34 -0.19 -3.90 0.87 -1.55 2.76 -2.25 -5.40 -1.29 -1.60 3.39 -0.56
Cytoplasm -2.72 1.10 0.19 3.48 -3.46 -1.35 -0.99 -5.02 -4.16 -0.90 -3.38 1.02 -3.80 0.70 -5.60
Cytoplasmic Foci -1.78 1.13 0.89 2.41 -2.15 -2.59 -1.63 -2.15 0.18 0.70 -3.24 0.51 -1.71 1.84 -1.97
Eisosomes -3.38 -6.24 -4.93 -0.69 3.00 -6.85 -10.12 -7.11 -0.92 5.03 -7.69 -8.61 -9.31 -0.66 2.37
Endoplasmic Reticulum -4.16 4.96 5.65 9.65 1.95 -5.64 -0.97 6.35 11.04 1.54 -6.54 6.23 9.19 14.95 1.01
Endosome -2.79 1.18 -2.90 0.98 -3.23 -2.71 -1.14 -2.64 -0.22 -1.69 -3.09 2.23 -2.93 0.46 -3.38
Golgi -1.53 -0.19 -2.02 0.86 -2.01 -2.40 -1.66 -1.77 0.40 -1.23 -2.70 -0.55 -2.06 0.94 -2.03
Lipid Particles -1.05 2.02 0.95 1.12 -4.30 -1.30 -1.37 0.22 1.56 1.49 -1.48 2.27 0.92 1.77 -2.98
Mitochondria -1.10 -0.60 -4.19 -3.48 -7.41 -1.94 -0.74 -2.50 -0.34 -2.11 -2.05 0.45 -3.75 -0.81 -5.15
Mitotic Spindle -1.36 -2.18 -4.43 -4.25 -4.34 -2.34 -0.83 -4.71 -3.49 -3.44 -2.36 0.39 -6.30 -5.01 -6.48
None -5.04 1.19 -0.16 5.11 -2.84 -0.05 3.55 -2.61 -2.45 -5.04 -4.54 3.44 -1.88 3.55 -5.60
Nuclear Periphery -6.07 -7.62 -5.04 -4.46 -4.48 -2.61 1.02 -3.89 -1.17 -4.84 -3.05 -0.62 -5.66 -4.11 -5.63
Nuclear Periphery Foci -3.23 1.19 -3.17 -1.88 -3.27 -1.66 -1.03 -1.56 -0.30 -1.07 -2.11 2.21 -2.94 -1.01 -3.51
Nucleolus -3.27 2.64 -0.37 3.51 -4.57 0.24 0.21 0.93 0.77 0.45 -0.54 5.36 1.55 2.63 -6.62
Nucleus 6.65 2.14 7.20 -1.44 7.80 5.64 2.05 9.04 3.29 1.56 8.55 3.04 11.75 1.79 10.68
Peroxisomes -1.02 2.73 1.01 1.86 -4.14 -0.93 -1.16 -0.28 0.72 1.16 -1.25 -0.57 0.66 1.90 0.67
Vacuole 0.33 1.10 0.53 0.67 -4.59 -4.07 -2.67 -3.92 -0.04 0.36 -2.18 0.84 -1.87 0.60 -5.07
Vacuole Periphery -2.32 -1.85 -3.33 -0.67 -3.09 -2.07 0.50 -0.91 0.83 -1.49 -2.19 1.04 -1.22 0.98 -3.01
Standard name
Human Ortholog
Description Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Trm8

Trm8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available