Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.12 0.11 0.09 0.07 0.09 0.07 0.06 0.07 0 0.12 0.14 0.17 0 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0 0 0.08 0.09 0.4 0.54 0.45 0.58 0.51 0.72 0.42 0.06 0 0 0 0.05 0.06 0 0 0 0 0 0
Nucleus 0.93 0.93 0.9 0.85 0.84 0.75 0.75 0.77 0.72 0.79 0.71 0.87 0.84 0.85 0.82 0.93 0.84 0.85 0.88 0.88 0.81 0.81 0.69 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0 0.06 0.12 0.09 0.16 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 1 0 0 1 0 0 4 2 4 1 7 2 0 0 0 0 0 0 3 1 1 1 7 8
Bud Neck 0 0 1 0 0 0 0 2 0 2 1 0 2 0 0 0 0 0 0 0 0 0 2 3
Bud Site 0 0 0 0 4 7 11 11 9 10 21 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 16 20 25 24 23 24 20 19 21 10 39 79 90 7 9 9 1 1 1 2 5 3
Endoplasmic Reticulum 0 0 1 1 1 0 0 0 0 0 0 0 3 0 1 3 3 4 1 0 0 1 1 2
Endosome 0 0 1 1 0 3 0 0 0 0 0 0 1 2 2 2 2 0 3 0 1 0 2 6
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 1
Mitochondria 85 0 7 14 20 104 171 118 171 161 205 113 19 0 2 5 9 9 1 1 2 5 9 12
Nucleus 268 115 209 142 189 194 236 202 212 246 204 234 281 486 442 212 147 131 252 122 215 231 336 339
Nuclear Periphery 1 0 0 0 0 2 1 0 0 0 2 2 0 0 0 1 2 1 0 0 0 0 2 3
Nucleolus 1 0 0 0 0 0 1 1 3 2 3 4 1 1 0 0 0 4 3 1 0 4 23 23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 0 5 2 2 3 1 2 0 2 1 3 5 2 0 1 2 0 2 5 14 9
Vac/Vac Membrane 2 6 2 1 5 5 5 3 3 2 4 3 3 9 6 10 18 13 12 8 31 24 78 63
Unique Cell Count 287 124 232 168 225 259 314 264 293 313 286 268 334 571 540 229 176 155 288 139 266 285 491 485
Labelled Cell Count 361 125 241 180 250 341 454 366 423 446 468 384 350 580 550 243 191 173 288 139 266 285 491 485


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 6.2 5.4 5.3 5.3 4.1 4.0 4.4 3.7 4.0 3.6 4.1 5.6 6.0 5.9 8.0 7.6 7.2 5.7 5.5 6.3
Std Deviation (1e-4) 0.7 1.1 1.0 1.6 2.1 1.2 1.7 1.3 1.1 1.3 1.2 1.3 1.8 1.2 1.3 1.4 1.5 1.4 1.4 1.0 1.9
Intensity Change (Log2) – – – -0.03 -0.01 -0.38 -0.44 -0.29 -0.53 -0.43 -0.57 -0.38 0.05 0.15 0.12 0.58 0.5 0.41 0.09 0.04 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.6 1.0 0.2 0.9 0 -0.4 0.2 -1.6 1.9 2.8 3.6 -1.9 -0.7 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 2.7 0 0 0 0 0 0 0 1.5 0 0 -0.6 1.1 1.4
Nucleus -1.7 -1.9 -4.4 -4.4 -4.0 -5.1 -3.6 -5.3 -1.0 -2.0 -1.9 -2.9 0.9 -2.0 -1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.4 4.3 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8033 0.8717 0.6843 -0.0528 0.8787 0.6676 1.8372 1.0035 1.1789 0.5785 0.8781 0.9759 0.8912 0.7737 0.6709 1.1709 0.6704 0.5569
Actin 0.0207 0 0.0025 0 0.01 0.0004 0.0428 0 0.0382 0.0008 0.0211 0.013 0.0155 0.0005 0.0001 0.0627 0.0257 0
Bud 0.0005 0 0.0004 0 0.0004 0 0.001 0 0.0003 0.0002 0.0001 0.0001 0.0005 0.0001 0.0002 0.0002 0.0001 0
Bud Neck 0.0011 0.0001 0.0015 0 0.0023 0.0004 0.0034 0.0001 0.0018 0.0003 0.0015 0.0007 0.0026 0.0001 0.0005 0.0012 0.0002 0.0005
Bud Periphery 0.0011 0 0.0007 0 0.0013 0 0.0025 0 0.0012 0.0001 0.0001 0.0001 0.0013 0.0002 0.0006 0.0003 0.0001 0.0001
Bud Site 0.0019 0.0003 0.0017 0 0.0017 0 0.0023 0 0.0014 0.0003 0.001 0.0001 0.002 0.0002 0.0003 0.001 0.0003 0
Cell Periphery 0.0002 0 0.0002 0 0.0003 0 0.0006 0 0.0013 0 0.0001 0 0.0004 0.0001 0 0.0001 0 0
Cytoplasm 0.0073 0.0003 0.0048 0 0.0006 0.0008 0.0137 0.0013 0.0032 0.0072 0.0184 0.0002 0.0075 0.0004 0.0029 0.0006 0.0016 0.0001
Cytoplasmic Foci 0.0055 0 0.006 0 0.0048 0 0.0319 0.0003 0.0086 0.0106 0.0152 0.0026 0.0197 0 0.0001 0.0086 0.0136 0
Eisosomes 0.0003 0 0 0 0.0001 0 0.0003 0 0.0002 0 0.0001 0 0.0002 0.0001 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0038 0 0.0015 0 0.0015 0.0003 0.0071 0 0.0068 0.0012 0.0007 0.0001 0.0049 0.0005 0.0001 0.0004 0.0003 0
Endosome 0.0237 0.0001 0.0143 0 0.0178 0.0002 0.0552 0.0001 0.0031 0.0194 0.0147 0.0019 0.0172 0.0005 0.0002 0.0349 0.0063 0
Golgi 0.0049 0 0.0041 0 0.0148 0 0.0058 0 0.0013 0.0014 0.0179 0.0021 0.0055 0.0001 0 0.0113 0.0051 0
Lipid Particles 0.0165 0 0.0052 0 0.0199 0 0.009 0 0.0031 0.0019 0.0121 0.0087 0.0188 0.0001 0 0.012 0.0142 0
Mitochondria 0.0096 0.0001 0.0061 0 0.0126 0.0003 0.0078 0.0002 0.0077 0.0012 0.0023 0.0013 0.0033 0.0078 0.0008 0.0041 0.0143 0.0001
None 0.0017 0.001 0.0005 0 0.0005 0.0001 0.0081 0.0019 0.0012 0.0006 0.0213 0.0002 0.0022 0.0002 0.0095 0.0004 0.0024 0
Nuclear Periphery 0.0286 0.0047 0.0198 0.0005 0.018 0.0041 0.0433 0.0071 0.0214 0.0088 0.0044 0.0005 0.0188 0.0081 0.0029 0.0033 0.0018 0.0006
Nucleolus 0.0088 0.01 0.009 0.0071 0.0284 0.0131 0.0085 0.005 0.0053 0.0025 0.0106 0.0077 0.0159 0.0045 0.0074 0.0053 0.0095 0.0098
Nucleus 0.8464 0.9759 0.8977 0.992 0.8412 0.9715 0.6983 0.9672 0.8779 0.9276 0.8218 0.9527 0.8417 0.9735 0.9718 0.8396 0.8822 0.9883
Peroxisomes 0.0034 0 0.0078 0 0.0116 0 0.004 0 0.001 0.0029 0.0111 0.0066 0.0099 0 0.0001 0.0043 0.0172 0
Punctate Nuclear 0.0077 0.0072 0.0107 0.0002 0.0067 0.0086 0.0407 0.0166 0.0114 0.0117 0.0248 0.0013 0.0054 0.0022 0.0019 0.0013 0.0044 0.0004
Vacuole 0.0032 0.0001 0.0021 0 0.0029 0.0001 0.009 0.0001 0.0023 0.0009 0.0005 0.0001 0.0048 0.0004 0.0004 0.0064 0.0002 0.0001
Vacuole Periphery 0.0032 0 0.0035 0 0.0025 0.0001 0.0048 0.0001 0.0013 0.0005 0.0002 0.0001 0.002 0.0003 0.0001 0.0018 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.868 8.7033 11.4813 11.8569 12.623 8.6494 10.2666 11.8787 9.9898 10.1797
Translational Efficiency 2.774 1.4296 1.2138 1.2852 0.8624 1.5158 1.1089 0.7008 1.083 0.9248

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
459 941 169 1639 2284 1578 372 1024 2743 2519 541 2663

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 731.37 776.46 866.99 890.08 740.56 750.99 972.18 902.94 739.02 760.50 939.32 895.03
Standard Deviation 89.36 117.30 131.75 112.53 85.75 107.11 108.71 125.47 86.44 111.71 126.20 117.84
Intensity Change Log 2 — 0.086310 0.245414 0.283334 — 0.020177 0.392607 0.286013 — 0.053416 0.321346 0.284683

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000099 0.000608 0.000905 0.000609 0.000056 0.000360 0.000822 0.000678 0.000063 0.000453 0.000848 0.000635
Bud Neck 0.001073 0.007757 0.003002 0.014183 0.002261 0.007461 0.007923 0.015799 0.002062 0.007571 0.006386 0.014804
Bud Site 0.000178 0.003160 0.000651 0.003129 0.000604 0.001944 0.001987 0.004820 0.000533 0.002398 0.001569 0.003779
Cell Periphery 0.000042 0.000158 0.000050 0.000111 0.000079 0.000112 0.000052 0.000162 0.000072 0.000129 0.000051 0.000130
Cytoplasm 0.008701 0.019835 0.001444 0.009546 0.011882 0.017661 0.002548 0.015351 0.011349 0.018473 0.002203 0.011778
Cytoplasmic Foci 0.002675 0.003676 0.000084 0.000912 0.001262 0.004199 0.000194 0.000255 0.001498 0.004004 0.000160 0.000659
Eisosomes 0.000018 0.000026 0.000035 0.000022 0.000009 0.000022 0.000039 0.000020 0.000010 0.000023 0.000038 0.000021
Endoplasmic Reticulum 0.001886 0.006736 0.008086 0.008063 0.002177 0.004705 0.008361 0.007693 0.002128 0.005464 0.008275 0.007921
Endosome 0.000584 0.001073 0.000208 0.001898 0.000411 0.003025 0.000455 0.000913 0.000440 0.002296 0.000378 0.001519
Golgi 0.000213 0.000681 0.000066 0.000417 0.000090 0.001090 0.000075 0.000252 0.000111 0.000937 0.000072 0.000354
Lipid Particles 0.000879 0.000820 0.000271 0.000215 0.000463 0.001891 0.000447 0.000168 0.000532 0.001491 0.000392 0.000197
Mitochondria 0.001225 0.003394 0.000698 0.003582 0.002353 0.004248 0.001661 0.002362 0.002165 0.003929 0.001360 0.003113
Mitotic Spindle 0.000895 0.001692 0.000162 0.012845 0.001241 0.006150 0.002000 0.009500 0.001183 0.004485 0.001426 0.011559
None 0.005726 0.011745 0.002917 0.004862 0.002225 0.007638 0.003119 0.005558 0.002811 0.009172 0.003056 0.005130
Nuclear Periphery 0.000594 0.000998 0.000392 0.003321 0.001507 0.001805 0.000875 0.001710 0.001355 0.001504 0.000724 0.002701
Nuclear Periphery Foci 0.000353 0.001142 0.000154 0.000536 0.000202 0.000912 0.000257 0.000257 0.000228 0.000998 0.000225 0.000429
Nucleolus 0.005490 0.010147 0.009486 0.003406 0.003254 0.009538 0.005022 0.002663 0.003628 0.009766 0.006417 0.003121
Nucleus 0.967859* 0.921381* 0.968995* 0.924654* 0.968107* 0.920075* 0.957908* 0.925187* 0.968065* 0.920563* 0.961372* 0.924859*
Peroxisomes 0.000849 0.001532 0.000313 0.000365 0.000230 0.002006 0.000621 0.000159 0.000334 0.001829 0.000525 0.000286
Vacuole 0.000577 0.002844 0.001975 0.006422 0.001317 0.004218 0.005300 0.005646 0.001193 0.003705 0.004262 0.006123
Vacuole Periphery 0.000083 0.000597 0.000105 0.000901 0.000270 0.000940 0.000333 0.000847 0.000238 0.000812 0.000262 0.000880

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.25 -9.95 -20.52 -1.44 2.48 -5.01 -10.82 -10.47 -5.85 0.87 -8.49 -13.89 -19.81 -6.21 2.74
Bud Neck -6.01 -4.12 -17.40 -5.98 -12.40 -5.78 -3.45 -11.63 -7.04 -3.80 -7.75 -3.83 -19.33 -9.65 -5.99
Bud Site -4.34 -3.97 -5.64 0.16 -4.34 -3.50 -3.12 -4.01 -2.38 -1.83 -5.16 -3.36 -6.20 -1.91 -2.92
Cell Periphery -5.97 -1.71 -5.57 3.21 -4.05 -2.33 1.25 -1.36 -0.51 -1.79 -4.50 1.19 -2.05 1.22 -3.00
Cytoplasm -3.41 3.76 -0.62 3.73 -9.00 -3.04 7.91 -0.46 2.46 -6.88 -4.36 8.96 -0.13 4.51 -11.00
Cytoplasmic Foci -0.74 2.12 1.72 3.41 -2.16 -4.48 3.41 3.36 6.38 -0.11 -4.42 4.04 3.02 6.84 -1.92
Eisosomes -2.57 -4.57 -4.04 -0.67 2.46 -6.67 -11.40 -13.55 -2.54 5.21 -8.56 -12.99 -18.31 -2.66 5.83
Endoplasmic Reticulum -8.71 -7.11 -19.14 -2.96 -0.05 -7.23 -10.99 -15.32 -8.83 0.66 -10.94 -12.97 -23.95 -9.21 1.03
Endosome -1.05 1.41 -1.01 -0.26 -2.31 -3.87 -1.11 -1.71 3.17 -1.11 -4.23 0.10 -2.09 2.41 -2.18
Golgi -1.07 0.91 -0.05 1.12 -1.55 -3.49 -0.13 -1.07 3.10 -1.08 -3.66 0.71 -1.27 3.07 -1.77
Lipid Particles 0.22 2.58 2.70 4.31 0.59 -3.59 -0.18 2.40 4.20 3.72 -3.68 1.39 3.55 5.04 3.91
Mitochondria -1.66 0.90 -1.94 0.32 -3.74 -1.84 0.85 0.03 2.25 -1.12 -2.05 1.21 -0.79 1.63 -2.57
Mitotic Spindle -0.81 0.88 -4.55 -4.38 -5.32 -3.62 -0.60 -3.66 -1.33 -3.23 -3.60 -0.17 -5.89 -3.35 -5.56
None -2.59 1.64 1.09 4.02 -1.05 -5.34 -1.36 -3.99 2.89 -2.15 -6.47 -0.38 -2.66 5.02 -2.01
Nuclear Periphery -2.68 1.05 -5.18 -4.49 -5.57 -0.99 1.67 -3.39 -2.51 -4.31 -0.75 2.50 -4.95 -4.46 -6.26
Nuclear Periphery Foci -1.56 1.00 0.16 2.45 -3.32 -2.77 -2.18 -2.80 1.29 -1.17 -3.77 -0.74 -2.56 2.61 -2.13
Nucleolus -3.11 -1.34 2.36 4.97 2.15 -6.80 -3.33 0.33 6.08 2.89 -7.88 -3.23 0.60 8.11 3.47
Nucleus 6.15 -0.21 7.12 -0.20 8.85 9.26 3.51 10.11 0.15 4.81 11.56 3.16 13.52 -0.17 7.65
Peroxisomes -1.21 1.39 1.41 2.72 0.18 -4.93 -1.34 1.13 5.17 1.78 -5.20 -0.67 0.94 5.61 1.39
Vacuole -2.98 -2.15 -7.41 -4.92 -6.41 -3.28 -4.18 -7.27 -4.39 -1.07 -4.20 -4.40 -10.14 -6.28 -2.68
Vacuole Periphery -2.72 -1.42 -2.95 0.16 -2.77 -2.50 -0.25 -0.99 1.27 -0.74 -3.09 -0.09 -2.10 1.25 -1.80
Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cwc2

Cwc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cwc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available