HO
Standard name
Human Ortholog
Description Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0.05 0
Bud Neck – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.92 – 0.99 0.98 0.96 0.95 – 0.76 0.88 0.68 0.78 – – 0.97 0.87 0.85 0.89 0.66 0.73 0.55 0.64 0.81 0.81
Endoplasmic Reticulum – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0.07 0.05 0.11 0 0 0 0 0 0
Endosome – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0.1 0 0 0 0 0
Golgi – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 – 0 0 0.07 0.05 – 0.27 0.13 0.47 0.24 – – 0 0 0 0 0 0.05 0.16 0 0 0
Nucleus – 0.05 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0.07 0 0 0 0
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 – 0 0 0 0 – 0.05 0.05 0 0.05 – – 0 0 0 0 0 0 0 0.14 0 0
Vac/Vac Membrane – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0.1 0.08 0.07 0.13 0 0.1 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 – 0 0 0 2 – – 0 0 2 0 0 3 1 0 0 0
Bud – 0 – 0 0 1 0 – 2 2 5 4 – – 0 0 1 1 0 6 1 0 2 0
Bud Neck – 1 – 5 10 2 14 – 8 5 5 13 – – 0 0 1 0 0 1 0 0 0 1
Bud Site – 0 – 0 0 0 0 – 1 0 1 1 – – 0 0 0 0 – – – – – –
Cell Periphery – 0 – 0 0 0 1 – 0 0 0 3 – – 1 2 2 3 0 1 0 0 0 0
Cytoplasm – 162 – 179 248 230 352 – 157 233 147 231 – – 77 188 159 188 4 129 21 14 45 61
Endoplasmic Reticulum – 2 – 0 0 0 0 – 0 0 2 1 – – 1 15 9 24 0 0 0 0 0 0
Endosome – 2 – 0 0 1 1 – 0 1 0 0 – – 0 3 5 6 0 3 0 0 1 0
Golgi – 0 – 0 1 0 0 – 0 0 0 0 – – 0 0 2 0 0 1 0 0 0 0
Mitochondria – 0 – 1 0 17 19 – 55 34 102 72 – – 0 7 5 5 0 8 6 0 0 1
Nucleus – 9 – 0 2 1 1 – 0 2 3 1 – – 0 2 7 3 0 11 0 0 0 1
Nuclear Periphery – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 – 0 0 0 0 – 0 0 1 0 – – 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 – 0 0 0 0 – 0 0 0 0 – – 0 0 0 1 0 0 0 0 0 0
SpindlePole – 0 – 1 1 1 8 – 10 12 5 15 – – 0 3 2 3 0 3 1 3 0 2
Vac/Vac Membrane – 4 – 1 3 1 1 – 2 1 1 3 – – 1 21 14 15 0 4 3 0 0 0
Unique Cell Count 176 181 253 240 372 207 266 217 298 79 215 186 212 7 178 39 23 56 76
Labelled Cell Count 180 187 265 254 397 235 290 272 346 80 241 209 249 7 178 39 23 56 76


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.6 – 4.7 5.2 4.2 4.2 – 3.5 4.1 3.1 3.5 – – 5.2 6.8 5.8 5.3 – 4.3 4.6
Std Deviation (1e-4) – 1.5 – 1.7 1.8 1.6 1.6 – 1.4 2.6 1.0 1.2 – – 1.8 3.8 1.4 1.3 – 1.2 1.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2323 -0.14 -0.1989 0.1641 -0.6178 -0.2345 -1.2139 -0.7947 -0.6958 -0.9752 -0.4899 -0.5219 -1.8093 -1.5015 -1.8465 -0.7761 -1.7835 -1.716
Actin 0.0151 0.0032 0.0049 0.0153 0.0014 0.0056 0.0065 0.0002 0.0098 0.0048 0.0044 0.0018 0.0054 0.0001 0.0016 0.0207 0.0009 0.0066
Bud 0.0052 0.0069 0.0034 0.0009 0.0007 0.0017 0.0011 0 0.004 0.0028 0.0027 0.0008 0.0015 0.0001 0.0003 0.0042 0.0001 0.0003
Bud Neck 0.001 0.0007 0.0023 0.0003 0.0001 0.0099 0.0033 0.0001 0.0044 0.0006 0.0008 0.0053 0.0007 0.0001 0.0001 0.0011 0 0.0014
Bud Periphery 0.0101 0.0041 0.0054 0.001 0.0015 0.0039 0.0038 0 0.0074 0.0023 0.007 0.0017 0.0021 0.0003 0.0005 0.008 0.0001 0.0007
Bud Site 0.0067 0.0159 0.0068 0.003 0.0002 0.0006 0.0062 0.0003 0.0057 0.0203 0.0128 0.0011 0.0022 0.001 0.0007 0.0104 0.0002 0.0001
Cell Periphery 0.0004 0.0002 0.0003 0.0001 0.0001 0.0002 0.0006 0.0001 0.0008 0.0002 0.0006 0.0002 0.0004 0.0001 0.0001 0.0006 0 0.0001
Cytoplasm 0.0257 0.0435 0.023 0.0117 0.0197 0.0458 0.0481 0.0801 0.0593 0.046 0.0272 0.0913 0.0518 0.0894 0.0405 0.0345 0.0149 0.0384
Cytoplasmic Foci 0.0283 0.0045 0.0074 0.0134 0.0118 0.0134 0.0147 0.0034 0.0119 0.0218 0.0077 0.0114 0.0287 0.0044 0.0149 0.0431 0.0194 0.0205
Eisosomes 0.0004 0.0002 0.0003 0.0002 0.0004 0.0003 0.0004 0.0001 0.0003 0.0001 0.0003 0.0001 0.0006 0.0001 0.0002 0.0004 0.0004 0.0001
Endoplasmic Reticulum 0.0024 0.0006 0.0006 0.0013 0.0004 0.0006 0.0029 0.0011 0.0025 0.0006 0.0033 0.0015 0.0047 0.002 0.001 0.009 0.0003 0.0008
Endosome 0.0108 0.0011 0.0071 0.0191 0.0021 0.0034 0.0178 0.0025 0.0091 0.0031 0.0231 0.0045 0.0162 0.0016 0.0079 0.057 0.0122 0.0036
Golgi 0.0065 0.0006 0.0023 0.0096 0.0011 0.0018 0.0055 0.0002 0.0034 0.0037 0.0074 0.001 0.0073 0.0001 0.0058 0.0459 0.004 0.0043
Lipid Particles 0.0161 0.0008 0.0024 0.0032 0.0015 0.0017 0.0096 0.0003 0.0084 0.0036 0.0181 0.0085 0.0287 0.0006 0.0039 0.0742 0.0071 0.0046
Mitochondria 0.0076 0.0013 0.0165 0.0081 0.0525 0.0079 0.0031 0.0002 0.0082 0.0022 0.0399 0.0019 0.0202 0.0003 0.0019 0.0231 0.0018 0.001
None 0.7876 0.8097 0.8794 0.8858 0.8748 0.8247 0.8452 0.8143 0.8397 0.8571 0.7966 0.7748 0.7452 0.726 0.904 0.5972 0.9128 0.9114
Nuclear Periphery 0.0087 0.0013 0.0036 0.0018 0.0006 0.0026 0.0029 0.001 0.0021 0.0003 0.0047 0.0013 0.0106 0.0087 0.001 0.0087 0.0002 0.0004
Nucleolus 0.0034 0.0015 0.0023 0.0002 0.0002 0.0018 0.001 0.0009 0.0006 0.0003 0.0188 0.0022 0.0031 0.0011 0.0008 0.0008 0.0001 0.0001
Nucleus 0.0222 0.0911 0.0141 0.0007 0.0006 0.0508 0.0048 0.0913 0.0116 0.0006 0.0056 0.0795 0.0367 0.16 0.001 0.0013 0.0001 0.0012
Peroxisomes 0.0202 0.0032 0.0078 0.0162 0.0287 0.0027 0.0135 0.0001 0.0066 0.0282 0.0051 0.0061 0.0081 0.0001 0.0123 0.0476 0.0242 0.0025
Punctate Nuclear 0.0168 0.0086 0.0071 0.0012 0.0011 0.0193 0.0042 0.0027 0.0007 0.0008 0.0041 0.0027 0.0064 0.0019 0.0007 0.0041 0.0009 0.0013
Vacuole 0.0031 0.0007 0.0025 0.004 0.0005 0.001 0.0042 0.0009 0.0026 0.0007 0.0075 0.002 0.0158 0.0014 0.0008 0.0051 0.0002 0.0004
Vacuole Periphery 0.0019 0.0002 0.0008 0.0028 0.0003 0.0004 0.0006 0.0001 0.0011 0.0001 0.0024 0.0004 0.0036 0.0003 0.0002 0.0029 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.1329 1.6804 5.8189 14.4672 109.6721 6.8885 2.6901 3.0579 10.0761 104.3
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p
Localization
Cell Percentages cytoplasm (24%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ho

Ho


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ho-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available