Standard name
Human Ortholog
Description Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.17 0.28 0.21 0.33 0.29 0.27 0.32 0.28 0.33 0.28 0.3 0.22 0.36 0.46 0.2 0.18 0.17 0.07 0.18 0.19 0.21 0.25 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.09 0.05 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.36 0.45 0.52 0.64 0.52 0.7 0.73 0.71 0.73 0.74 0.75 0.81 0.06 0.06 0.05 0.1 0 0.07 0 0 0 0 0 0
Nucleus 0.45 0.3 0.37 0.41 0.22 0.23 0.16 0.2 0.25 0.24 0.19 0.14 0.4 0.23 0.15 0.42 0.49 0.48 0.55 0.46 0.39 0.47 0.34 0.18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.15
Vac/Vac Membrane 0.24 0.37 0.13 0.06 0.14 0.08 0.06 0 0.06 0 0 0.05 0.42 0.46 0.46 0.27 0.27 0.31 0.28 0.24 0.3 0.21 0.21 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Bud 2 0 0 0 1 5 3 7 0 2 5 1 0 0 1 2 0 0 6 2 2 3 9 23
Bud Neck 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0 2 0 0 0 3 10
Bud Site 2 0 0 1 1 1 0 6 7 6 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 46 18 28 29 61 83 97 172 74 89 66 81 72 126 188 33 12 18 31 21 22 40 89 89
Endoplasmic Reticulum 0 1 0 0 0 3 0 0 1 0 0 1 0 0 1 17 6 5 0 0 0 0 2 4
Endosome 23 0 0 1 4 0 0 1 0 1 0 0 9 7 7 13 4 10 5 3 0 3 9 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 3
Mitochondria 120 47 52 89 98 202 261 380 194 199 175 218 18 21 20 16 3 8 7 1 5 2 11 14
Nucleus 148 32 37 57 41 65 59 107 67 64 45 39 128 81 61 69 33 52 262 55 46 89 124 75
Nuclear Periphery 0 1 0 1 0 1 1 2 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 6 1 0 0 1 0 0 0 0 2 0 0 0 1 1 6 0 2 0 0 0 1 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 1 1 5 0 0 0 1 0 0 0 6 2 2 11 3 2 6 24 60
Vac/Vac Membrane 81 39 13 9 26 23 21 24 16 9 8 14 134 163 187 45 18 33 133 29 35 40 75 98
Unique Cell Count 331 105 100 139 187 288 359 536 266 269 232 269 322 354 408 166 67 108 473 122 120 192 362 413
Labelled Cell Count 428 139 130 187 233 384 443 706 361 373 302 357 361 400 467 207 78 131 473 122 120 192 362 413


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.9 3.6 3.4 3.7 3.1 2.8 3.1 3.1 2.7 2.9 2.7 4.9 4.6 4.7 5.0 6.0 7.1 4.7 4.5 5.3
Std Deviation (1e-4) 0.6 0.6 0.9 0.7 1.1 1.0 0.9 1.2 1.3 1.2 1.2 1.0 0.9 1.0 1.5 1.3 1.3 1.8 1.1 1.1 1.8
Intensity Change (Log2) – – – -0.06 0.03 -0.21 -0.37 -0.19 -0.22 -0.38 -0.29 -0.4 0.44 0.35 0.39 0.49 0.74 0.99 0.4 0.32 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.7 2.3 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5894 2.1662 1.7678 1.696 1.5386 2.0063 -0.8928 0.3564 -0.0287 0.141 -0.4983 0.1096 1.673 1.8244 1.7528 1.7194 1.4095 1.7932
Actin 0.0002 0.0006 0.0003 0.0002 0.0002 0.0003 0.0003 0.0001 0.0011 0.0001 0.0002 0.0002 0.0401 0.0004 0.0012 0.0007 0.0036 0.0042
Bud 0.0001 0.0001 0.0014 0.0001 0.0008 0.0019 0.0001 0 0.0001 0 0 0 0.0007 0.0001 0.0001 0.0002 0.0001 0.0003
Bud Neck 0.0006 0.0003 0.0005 0.001 0.0006 0.0021 0.0003 0.0002 0.0003 0.0003 0.0002 0.002 0.0081 0.0004 0.0004 0.0008 0.0005 0.0048
Bud Periphery 0 0.0001 0.0002 0 0.0004 0.0007 0.0003 0 0.0002 0 0 0 0.0007 0.0001 0.0001 0.0001 0.0001 0.0003
Bud Site 0.0002 0.0006 0.0028 0.0003 0.0003 0.0002 0.0004 0.0004 0.0007 0.0001 0 0.0001 0.0074 0.0009 0.0005 0.0003 0.0003 0.0004
Cell Periphery 0.0001 0 0.0002 0 0.0001 0 0.0006 0 0.0001 0 0 0 0.0003 0.0001 0 0 0 0.0001
Cytoplasm 0.0883 0.1016 0.0941 0.0203 0.0767 0.066 0.1277 0.1565 0.1282 0.2499 0.1244 0.0732 0.066 0.1039 0.0912 0.1511 0.0597 0.0543
Cytoplasmic Foci 0.0107 0.0068 0.0022 0.0011 0.01 0.0047 0.0018 0.0012 0.0058 0.0052 0.0008 0.0021 0.0147 0.0021 0.0033 0.0015 0.0044 0.0083
Eisosomes 0.0001 0 0 0 0.0002 0 0.0004 0 0.0001 0 0 0 0.0005 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0041 0.0017 0.0023 0.0004 0.0027 0.0014 0.0053 0.0035 0.0037 0.0069 0.0012 0.0013 0.0023 0.0073 0.0018 0.0037 0.0062 0.0018
Endosome 0.0065 0.0027 0.002 0.0004 0.0018 0.0032 0.0086 0.0011 0.0094 0.007 0.0007 0.0118 0.0039 0.0051 0.0017 0.0021 0.0268 0.004
Golgi 0.0001 0.0035 0.0001 0.0001 0 0.0013 0.0011 0 0.0032 0.0001 0 0.0155 0.0026 0.0001 0.0001 0.0001 0.0091 0.0011
Lipid Particles 0.0004 0.0001 0.0003 0.0001 0.0006 0.0008 0.0205 0.0001 0.0077 0.0003 0 0.0002 0.0041 0.0004 0.0002 0.0001 0.0142 0.0033
Mitochondria 0.0003 0.0004 0.001 0.0002 0.0402 0.0009 0.0625 0.0002 0.0157 0.0005 0.0006 0.0005 0.0078 0.0005 0.0005 0.0009 0.002 0.0007
None 0.0657 0.0057 0.0715 0.0562 0.086 0.0436 0.2332 0.3062 0.1763 0.3075 0.2786 0.1341 0.117 0.0946 0.1629 0.1168 0.1079 0.0588
Nuclear Periphery 0.0143 0.0156 0.0165 0.005 0.0095 0.0098 0.0141 0.011 0.0128 0.0174 0.0081 0.0082 0.0102 0.0155 0.0105 0.0203 0.0187 0.0081
Nucleolus 0.0028 0.0027 0.0036 0.0043 0.0082 0.0049 0.0026 0.0019 0.0024 0.0013 0.0009 0.0037 0.0046 0.0029 0.0028 0.002 0.0021 0.0055
Nucleus 0.7775 0.8439 0.7649 0.8083 0.7508 0.8237 0.4968 0.5114 0.6122 0.392 0.5803 0.7346 0.687 0.7498 0.7039 0.6769 0.7184 0.8045
Peroxisomes 0.0001 0.0006 0.0001 0 0.0021 0.0008 0.0005 0 0.0014 0.0001 0 0 0.0031 0.0001 0.0002 0.0002 0.0116 0.0034
Punctate Nuclear 0.0235 0.0113 0.034 0.1017 0.0069 0.0328 0.0091 0.0049 0.0166 0.0074 0.0033 0.0116 0.017 0.0071 0.0177 0.0213 0.0099 0.0349
Vacuole 0.004 0.0013 0.0018 0.0002 0.0013 0.0007 0.0097 0.001 0.0018 0.0031 0.0005 0.0006 0.0014 0.0082 0.0007 0.0006 0.0033 0.0009
Vacuole Periphery 0.0004 0.0002 0.0003 0.0001 0.0004 0.0002 0.0042 0.0001 0.0004 0.0005 0.0002 0.0002 0.0004 0.0005 0.0002 0.0003 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2118 5.7306 5.3154 10.9786 5.1947 12.013 9.7777 8.0658 12.3757 11.8859
Translational Efficiency 1.4224 1.6627 2.0379 0.8592 1.899 0.9823 1.2095 1.3335 0.995 0.9989

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
Localization
Cell Percentages nucleus (36%), cytoplasm (11%), mixed (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mfg1

Mfg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mfg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available