Standard name | |
---|---|
Human Ortholog | |
Description | Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 1 | 3 | 4 | 11 | 17 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | 6 | 12 | 10 | 15 | 20 | 23 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 2 | 6 | 7 | 12 | 10 | 25 | 10 | 7 | 9 | 8 | 13 | 20 | 56 | 78 | 25 | 36 | 26 | 7 | 2 | 2 | 9 | 12 | 11 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 11 | 8 | 0 | 0 | 1 | 0 | 0 | 2 |
Endosome | 7 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 2 | 5 | 4 | 0 | 1 | 0 | 9 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 2 | 0 |
Mitochondria | 24 | 4 | 1 | 4 | 2 | 29 | 66 | 26 | 66 | 80 | 97 | 82 | 0 | 0 | 0 | 3 | 7 | 3 | 6 | 0 | 2 | 0 | 7 | 7 |
Nucleus | 265 | 103 | 271 | 235 | 336 | 326 | 466 | 456 | 343 | 417 | 504 | 701 | 339 | 444 | 409 | 68 | 81 | 47 | 283 | 108 | 264 | 176 | 441 | 358 |
Nuclear Periphery | 5 | 0 | 2 | 2 | 2 | 1 | 10 | 11 | 3 | 4 | 3 | 14 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 4 | 0 | 3 | 2 | 4 | 20 | 61 | 62 | 70 | 91 | 138 | 193 | 0 | 4 | 4 | 1 | 0 | 1 | 4 | 1 | 2 | 0 | 10 | 15 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 2 | 6 | 0 | 0 | 0 | 5 | 0 | 4 | 4 | 11 | 12 |
Vac/Vac Membrane | 26 | 18 | 5 | 0 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 5 | 2 | 8 | 45 | 70 | 37 | 17 | 9 | 10 | 14 | 43 | 72 |
Unique Cell Count | 316 | 116 | 286 | 242 | 355 | 363 | 547 | 504 | 419 | 504 | 613 | 858 | 362 | 508 | 504 | 129 | 179 | 106 | 344 | 126 | 298 | 217 | 557 | 513 |
Labelled Cell Count | 341 | 127 | 290 | 250 | 360 | 391 | 641 | 582 | 504 | 621 | 775 | 1034 | 365 | 512 | 509 | 153 | 209 | 128 | 344 | 126 | 298 | 217 | 557 | 513 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.6 | 6.6 | 6.2 | 6.7 | 5.9 | 5.8 | 5.9 | 5.6 | 5.4 | 5.3 | 5.6 | 7.9 | 7.9 | 7.5 | 8.9 | 8.6 | 9.9 | 7.2 | 7.3 | 7.9 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.5 | 1.5 | 1.7 | 1.3 | 1.5 | 1.5 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 | 1.7 | 1.7 | 1.9 | 2.1 | 16.2 | 1.0 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.02 | -0.18 | -0.19 | -0.17 | -0.26 | -0.3 | -0.32 | -0.24 | 0.24 | 0.26 | 0.18 | 0.42 | 0.37 | 0.57 | 0.11 | 0.15 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 3.1 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 1.0 | 0.5 | 1.8 | -0.1 | -0.4 | -0.3 | 0 | 0 | 2.2 | 4.5 | 5.9 | 6.2 | 6.6 | 7.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.6 | 5.9 | 3.6 | 6.8 | 6.9 | 6.9 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.3 | -0.1 | -2.3 | -4.1 | -2.1 | -5.0 | -4.8 | -5.1 | -5.3 | -0.6 | -3.3 | -5.3 | -10.2 | -12.1 | -11.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.1 | 5.2 | 5.5 | 6.7 | 7.1 | 8.2 | 8.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9.6 | 10.7 | 9.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5021 | 3.5295 | 3.1633 | 3.0757 | 2.7628 | 3.1076 | 2.3644 | 2.8514 | 2.2194 | 2.296 | 2.2443 | 2.3399 | 2.0357 | 2.192 | 1.6561 | 1.8468 | 1.5036 | 1.4524 |
Actin | 0.0201 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0.0631 | 0 | 0.0006 | 0.0038 | 0.0001 | 0.0007 | 0.0381 | 0.0001 | 0.0033 | 0.0114 | 0.0183 | 0.0001 |
Bud | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0.0004 | 0.0011 | 0.0001 | 0.0004 | 0.0008 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0.0002 |
Bud Neck | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0024 | 0.0107 | 0.0001 | 0.0001 | 0.0005 | 0.0006 | 0.0015 | 0.0078 | 0.0001 | 0.0018 | 0.0023 | 0.0007 | 0.0012 |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0024 | 0 | 0.0004 | 0.0036 | 0.0001 | 0.0006 | 0.0019 | 0.0001 | 0.0001 | 0.0014 | 0.0002 | 0.0001 |
Bud Site | 0.0001 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0257 | 0.0001 | 0.0016 | 0.0011 | 0.0001 | 0.0011 | 0.0035 | 0.0001 | 0.0055 | 0.0141 | 0.0002 | 0.0004 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Cytoplasm | 0.0049 | 0.0949 | 0.0344 | 0.0259 | 0.0247 | 0.012 | 0.007 | 0.0359 | 0.0122 | 0.0125 | 0.0027 | 0.0085 | 0.0026 | 0.0142 | 0.0052 | 0.002 | 0.0033 | 0.0051 |
Cytoplasmic Foci | 0.0008 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0451 | 0.0001 | 0.0026 | 0.0007 | 0 | 0.0023 | 0.0273 | 0.0001 | 0.0013 | 0.0032 | 0.0033 | 0.0026 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0003 | 0.0007 | 0.001 | 0.0004 | 0.0016 | 0.0003 | 0.0086 | 0.0005 | 0.0002 | 0.0007 | 0.0003 | 0.0005 | 0.0041 | 0.0004 | 0.0002 | 0.0011 | 0.0005 | 0.0001 |
Endosome | 0.0012 | 0.0004 | 0.0002 | 0.0005 | 0.0019 | 0.0004 | 0.0664 | 0.0002 | 0.0029 | 0.0106 | 0.0001 | 0.0069 | 0.0218 | 0.0002 | 0.0011 | 0.0082 | 0.0049 | 0.0021 |
Golgi | 0.0011 | 0 | 0 | 0 | 0 | 0 | 0.0186 | 0 | 0.0023 | 0.0052 | 0 | 0.0061 | 0.0105 | 0 | 0.0013 | 0.0044 | 0.0074 | 0.0006 |
Lipid Particles | 0.0008 | 0 | 0 | 0 | 0.0001 | 0 | 0.0199 | 0 | 0.0027 | 0.0022 | 0 | 0.0115 | 0.0167 | 0 | 0.0007 | 0.0075 | 0.0129 | 0.0003 |
Mitochondria | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0127 | 0.0002 | 0.0026 | 0.0073 | 0.0006 | 0.0043 | 0.013 | 0.001 | 0.0015 | 0.0073 | 0.0013 | 0.0006 |
None | 0.0166 | 0.0165 | 0.0124 | 0.0017 | 0.0149 | 0.0068 | 0.0041 | 0.0021 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0019 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0027 | 0.0042 | 0.0049 | 0.0032 | 0.0291 | 0.0033 | 0.0204 | 0.0043 | 0.0045 | 0.0042 | 0.0099 | 0.0091 | 0.0188 | 0.0048 | 0.0013 | 0.0033 | 0.0029 | 0.0012 |
Nucleolus | 0.0048 | 0.004 | 0.0033 | 0.0018 | 0.0069 | 0.0103 | 0.0113 | 0.0026 | 0.0025 | 0.0025 | 0.0091 | 0.0071 | 0.0046 | 0.0031 | 0.0025 | 0.0033 | 0.0086 | 0.0065 |
Nucleus | 0.929 | 0.8644 | 0.9389 | 0.9634 | 0.905 | 0.9504 | 0.6142 | 0.9504 | 0.9557 | 0.9355 | 0.974 | 0.9321 | 0.7953 | 0.9675 | 0.9705 | 0.923 | 0.929 | 0.976 |
Peroxisomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0239 | 0 | 0.0044 | 0.0004 | 0 | 0.0016 | 0.0138 | 0 | 0.0002 | 0.0017 | 0.0034 | 0.0008 |
Punctate Nuclear | 0.0159 | 0.0117 | 0.0038 | 0.0022 | 0.0135 | 0.0128 | 0.0114 | 0.0028 | 0.0029 | 0.0022 | 0.001 | 0.0033 | 0.0107 | 0.0066 | 0.0021 | 0.0015 | 0.0021 | 0.0017 |
Vacuole | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0007 | 0.0004 | 0.0209 | 0.0003 | 0.0005 | 0.0033 | 0.0006 | 0.0012 | 0.0041 | 0.0007 | 0.0002 | 0.0015 | 0.0002 | 0.0003 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0105 | 0.0001 | 0.0002 | 0.0021 | 0.0003 | 0.0007 | 0.0022 | 0.0006 | 0.0001 | 0.0006 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.0402 | 23.2479 | 12.9382 | 18.3993 | 19.2675 | 14.8524 | 17.6295 | 23.5141 | 20.4077 | 23.702 |
Translational Efficiency | 0.9579 | 0.7359 | 1.1812 | 0.8098 | 0.8919 | 1.6515 | 1.3839 | 0.9421 | 1.0256 | 1.0396 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1626 | 673 | 1702 | 95 | 848 | 1831 | 271 | 2634 | 2474 | 2504 | 1973 | 2729 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 837.89 | 903.94 | 991.39 | 1049.14 | 779.14 | 844.28 | 1112.71 | 1038.99 | 817.75 | 860.31 | 1008.05 | 1039.34 |
Standard Deviation | 116.12 | 138.69 | 134.41 | 146.78 | 120.02 | 114.51 | 128.75 | 151.83 | 120.73 | 124.33 | 140.02 | 151.67 |
Intensity Change Log 2 | — | 0.109466 | 0.242692 | 0.324374 | — | 0.115839 | 0.514123 | 0.415227 | — | 0.112540 | 0.379857 | 0.368865 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000200 | 0.000584 | 0.000539 | 0.001278 | 0.000331 | 0.000216 | 0.001310 | 0.001066 | 0.000245 | 0.000315 | 0.000645 | 0.001073 |
Bud Neck | 0.005948 | 0.006593 | 0.010371 | 0.008204 | 0.004158 | 0.007236 | 0.004525 | 0.037977 | 0.005334 | 0.007063 | 0.009568 | 0.036941 |
Bud Site | 0.000868 | 0.002441 | 0.002127 | 0.002149 | 0.000850 | 0.001983 | 0.001381 | 0.018003 | 0.000862 | 0.002106 | 0.002025 | 0.017451 |
Cell Periphery | 0.000104 | 0.000149 | 0.000102 | 0.000131 | 0.000244 | 0.000138 | 0.000105 | 0.000165 | 0.000152 | 0.000141 | 0.000102 | 0.000163 |
Cytoplasm | 0.006598 | 0.029569 | 0.013707 | 0.021538 | 0.008084 | 0.028839 | 0.010527 | 0.109566* | 0.007107 | 0.029035 | 0.013270 | 0.106501* |
Cytoplasmic Foci | 0.001186 | 0.004863 | 0.000396 | 0.000158 | 0.001304 | 0.003884 | 0.000489 | 0.000614 | 0.001227 | 0.004147 | 0.000409 | 0.000598 |
Eisosomes | 0.000017 | 0.000026 | 0.000020 | 0.000043 | 0.000019 | 0.000017 | 0.000065 | 0.000017 | 0.000017 | 0.000020 | 0.000026 | 0.000018 |
Endoplasmic Reticulum | 0.004419 | 0.007884 | 0.004928 | 0.014328 | 0.004569 | 0.004174 | 0.013805 | 0.002457 | 0.004471 | 0.005171 | 0.006147 | 0.002871 |
Endosome | 0.000433 | 0.000868 | 0.000380 | 0.000538 | 0.000371 | 0.001288 | 0.000566 | 0.002475 | 0.000412 | 0.001175 | 0.000406 | 0.002408 |
Golgi | 0.000219 | 0.000699 | 0.000103 | 0.000072 | 0.000104 | 0.000570 | 0.000065 | 0.000368 | 0.000179 | 0.000605 | 0.000098 | 0.000358 |
Lipid Particles | 0.000232 | 0.001519 | 0.000128 | 0.000361 | 0.000185 | 0.000647 | 0.000711 | 0.000224 | 0.000216 | 0.000881 | 0.000208 | 0.000229 |
Mitochondria | 0.000956 | 0.002323 | 0.001332 | 0.001127 | 0.000930 | 0.003269 | 0.000865 | 0.005703 | 0.000947 | 0.003015 | 0.001268 | 0.005543 |
Mitotic Spindle | 0.004251 | 0.006161 | 0.002466 | 0.000437 | 0.001857 | 0.009445 | 0.000213 | 0.030042 | 0.003431 | 0.008562 | 0.002156 | 0.029011 |
None | 0.001518 | 0.003231 | 0.003883 | 0.002822 | 0.005211 | 0.003480 | 0.002890 | 0.013894 | 0.002784 | 0.003413 | 0.003747 | 0.013508 |
Nuclear Periphery | 0.000641 | 0.001510 | 0.000837 | 0.001196 | 0.000412 | 0.001844 | 0.000946 | 0.002245 | 0.000563 | 0.001754 | 0.000852 | 0.002208 |
Nuclear Periphery Foci | 0.000415 | 0.000632 | 0.000148 | 0.000316 | 0.000297 | 0.000886 | 0.000271 | 0.000174 | 0.000375 | 0.000817 | 0.000165 | 0.000179 |
Nucleolus | 0.004811 | 0.003219 | 0.001068 | 0.001024 | 0.008270 | 0.004569 | 0.001899 | 0.001266 | 0.005996 | 0.004206 | 0.001182 | 0.001258 |
Nucleus | 0.965542* | 0.919795* | 0.954229* | 0.926986* | 0.961222* | 0.921225* | 0.941780* | 0.762203* | 0.964061* | 0.920840* | 0.952519* | 0.767939* |
Peroxisomes | 0.000519 | 0.002083 | 0.000095 | 0.000110 | 0.000278 | 0.000852 | 0.000202 | 0.000565 | 0.000436 | 0.001183 | 0.000109 | 0.000549 |
Vacuole | 0.000985 | 0.005520 | 0.003050 | 0.017068 | 0.000894 | 0.004731 | 0.017276 | 0.010381 | 0.000954 | 0.004943 | 0.005004 | 0.010614 |
Vacuole Periphery | 0.000137 | 0.000332 | 0.000089 | 0.000113 | 0.000410 | 0.000706 | 0.000109 | 0.000595 | 0.000231 | 0.000605 | 0.000092 | 0.000578 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.22 | -11.42 | -9.93 | -5.24 | -6.42 | 1.20 | -7.38 | -1.25 | -2.06 | 5.17 | -2.22 | -11.67 | -2.21 | -1.53 | 1.66 |
Bud Neck | -1.56 | -6.61 | -3.50 | -2.54 | 0.74 | -3.45 | -3.83 | -14.89 | -12.09 | -13.33 | -2.74 | -7.85 | -14.43 | -12.66 | -8.91 |
Bud Site | -3.21 | -3.92 | -6.44 | -0.98 | -2.94 | -2.29 | -2.74 | -7.02 | -5.62 | -4.45 | -3.94 | -4.46 | -7.38 | -5.37 | -5.30 |
Cell Periphery | -2.20 | -0.00 | -3.59 | -0.89 | -3.84 | 1.17 | 1.23 | 1.62 | 2.31 | 2.42 | 0.34 | 1.36 | 1.63 | 2.93 | 1.01 |
Cytoplasm | -5.84 | -3.41 | -3.59 | -0.27 | -2.79 | -7.50 | -0.08 | -25.89 | -17.84 | -25.90 | -10.41 | -3.21 | -29.24 | -18.31 | -27.07 |
Cytoplasmic Foci | -3.18 | 1.92 | 1.79 | 4.03 | -0.49 | -2.06 | 1.36 | 1.47 | 5.43 | 0.42 | -4.10 | 2.38 | 2.31 | 6.76 | -0.57 |
Eisosomes | -5.02 | -6.93 | -8.00 | -5.93 | -6.77 | 0.24 | -10.60 | -1.19 | -1.51 | 10.52 | -2.88 | -11.18 | -4.78 | -0.70 | 7.77 |
Endoplasmic Reticulum | -6.40 | -4.58 | -6.84 | -4.23 | -5.76 | 0.80 | -10.27 | 4.96 | 4.83 | 12.24 | -2.93 | -8.38 | 4.93 | 7.15 | 12.10 |
Endosome | -3.41 | 0.29 | -3.41 | 0.11 | -3.80 | -4.03 | -2.83 | -1.71 | 1.34 | -0.24 | -4.90 | -0.41 | -1.75 | 1.26 | -1.61 |
Golgi | -1.34 | 1.21 | 0.78 | 1.66 | -1.29 | -2.71 | 0.39 | -0.91 | 2.40 | -1.42 | -2.46 | 1.22 | 0.44 | 2.87 | -1.19 |
Lipid Particles | -1.99 | 1.30 | -4.58 | 1.15 | -6.03 | -3.50 | -5.47 | -2.05 | 2.79 | 4.85 | -3.34 | -0.47 | -1.83 | 2.93 | -2.23 |
Mitochondria | -1.47 | -2.20 | -2.29 | 0.94 | 0.32 | -3.78 | -0.83 | -6.39 | -0.65 | -7.52 | -4.12 | -2.45 | -7.43 | -1.03 | -5.98 |
Mitotic Spindle | -0.97 | 0.43 | -1.03 | -0.42 | -1.20 | -3.73 | 0.47 | -10.11 | -6.36 | -10.60 | -3.05 | 0.12 | -9.60 | -6.88 | -9.80 |
None | -3.02 | -2.30 | -2.09 | -1.54 | -1.51 | 1.71 | 3.02 | -6.06 | -11.18 | -13.24 | -1.12 | -0.34 | -13.14 | -12.21 | -10.55 |
Nuclear Periphery | -1.47 | -3.54 | -7.35 | -1.62 | -6.06 | -4.45 | -8.33 | -20.53 | -5.32 | -13.49 | -4.11 | -5.96 | -19.47 | -5.89 | -16.66 |
Nuclear Periphery Foci | -0.94 | 1.75 | -0.97 | 0.31 | -2.71 | -1.61 | -0.07 | 1.81 | 2.64 | 2.52 | -1.81 | 1.89 | 2.55 | 3.09 | 1.10 |
Nucleolus | 1.96 | 6.20 | 6.20 | 4.84 | 0.74 | 2.97 | 6.25 | 6.29 | 4.77 | -0.15 | 2.45 | 8.89 | 8.45 | 5.66 | -1.59 |
Nucleus | 6.85 | 4.21 | 6.97 | 3.85 | 6.12 | 6.75 | 4.51 | 26.57 | 19.18 | 15.31 | 10.31 | 5.33 | 32.15 | 20.10 | 28.39 |
Peroxisomes | -1.73 | 2.25 | 2.14 | 2.15 | -0.82 | -2.54 | 0.68 | 0.90 | 3.08 | 0.52 | -2.43 | 2.52 | 1.83 | 3.38 | -0.44 |
Vacuole | -4.58 | -7.52 | -5.48 | -4.84 | -5.18 | -5.40 | -5.62 | -15.67 | -11.47 | 0.81 | -7.14 | -8.68 | -16.66 | -11.90 | -10.71 |
Vacuole Periphery | -1.71 | 1.44 | -0.21 | 1.69 | -3.17 | -1.06 | 1.37 | 0.87 | 2.73 | -2.55 | -2.50 | 1.76 | 0.11 | 2.85 | -3.22 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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