| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 4 | 4 |
| Bud | 1 | 3 | 2 | 2 | 1 | 3 | 1 | 3 | 5 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 5 | 9 | 18 |
| Bud Neck | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 5 |
| Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 2 | 1 | 5 | 1 | 0 | 0 | 0 | 1 | 1 | 4 |
| Cytoplasm | 286 | 296 | 214 | 99 | 152 | 140 | 150 | 167 | 90 | 120 | 68 | 92 | 771 | 763 | 866 | 442 | 372 | 282 | 255 | 290 | 186 | 393 | 416 | 426 |
| Endoplasmic Reticulum | 2 | 4 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 3 | 22 | 33 | 21 | 2 | 0 | 1 | 0 | 1 | 4 |
| Endosome | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 1 | 3 | 0 | 0 | 4 | 5 | 7 |
| Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 |
| Mitochondria | 18 | 4 | 5 | 16 | 24 | 54 | 90 | 90 | 87 | 93 | 84 | 105 | 2 | 1 | 1 | 8 | 0 | 1 | 12 | 10 | 15 | 11 | 12 | 13 |
| Nucleus | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 4 | 2 | 0 | 3 | 1 | 4 | 5 | 0 | 0 | 0 | 0 | 1 | 2 |
| Nuclear Periphery | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 1 | 1 | 0 | 6 | 11 | 15 |
| Vac/Vac Membrane | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 8 | 2 | 3 | 4 | 1 | 4 | 4 | 14 |
| Unique Cell Count | 295 | 304 | 215 | 103 | 163 | 157 | 194 | 213 | 145 | 173 | 121 | 155 | 773 | 767 | 871 | 455 | 387 | 288 | 291 | 320 | 216 | 444 | 482 | 535 |
| Labelled Cell Count | 310 | 313 | 224 | 122 | 182 | 197 | 246 | 264 | 186 | 218 | 158 | 210 | 777 | 767 | 876 | 487 | 435 | 316 | 291 | 320 | 216 | 444 | 482 | 535 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.6 | 4.3 | 3.6 | 3.3 | 3.6 | 3.1 | 2.8 | 3.2 | 3.0 | 2.7 | 2.9 | 3.0 | 4.7 | 4.7 | 4.9 | 5.0 | 6.1 | 5.4 | 3.9 | 4.1 | 4.7 |
| Std Deviation (1e-4) | 0.6 | 0.7 | 0.5 | 1.1 | 1.8 | 1.3 | 1.0 | 1.0 | 0.8 | 1.1 | 0.6 | 1.1 | 1.1 | 1.4 | 1.4 | 1.1 | 1.6 | 1.0 | 1.3 | 1.3 | 1.3 |
| Intensity Change (Log2) | – | – | – | -0.1 | 0.01 | -0.19 | -0.36 | -0.17 | -0.25 | -0.39 | -0.29 | -0.27 | 0.38 | 0.41 | 0.45 | 0.48 | 0.77 | 0.6 | 0.14 | 0.2 | 0.39 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | 0.3073 | 0.0012 | 0.0034 | 0.0002 | 0.0007 | 0.0004 | – | – | – | – | – | – | 0.0663 | 0.0008 | 0.0023 | 0.0003 | 0.0028 | 0.009 |
| Bud | 0.0031 | 0.0003 | 0.0005 | 0 | 0.0002 | 0 | – | – | – | – | – | – | 0.0037 | 0.0013 | 0.0018 | 0.0047 | 0.0088 | 0.0019 |
| Bud Neck | 0.0033 | 0 | 0.0005 | 0 | 0.0002 | 0.0008 | – | – | – | – | – | – | 0.0046 | 0.0003 | 0.0002 | 0.0039 | 0.0012 | 0.0025 |
| Bud Periphery | 0.0079 | 0.0004 | 0.0012 | 0 | 0.0002 | 0 | – | – | – | – | – | – | 0.0069 | 0.0005 | 0.0039 | 0.004 | 0.0201 | 0.0054 |
| Bud Site | 0.0275 | 0.0005 | 0.0031 | 0 | 0.0002 | 0 | – | – | – | – | – | – | 0.0155 | 0.0054 | 0.0023 | 0.0168 | 0.0014 | 0.0006 |
| Cell Periphery | 0.0021 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0001 | – | – | – | – | – | – | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 |
| Cytoplasm | 0.019 | 0.1248 | 0.227 | 0.1666 | 0.1401 | 0.3364 | – | – | – | – | – | – | 0.0886 | 0.2638 | 0.2097 | 0.0929 | 0.1372 | 0.2082 |
| Cytoplasmic Foci | 0.0236 | 0.0252 | 0.0141 | 0.0016 | 0.0374 | 0.0063 | – | – | – | – | – | – | 0.0372 | 0.007 | 0.006 | 0.0323 | 0.0078 | 0.0071 |
| Eisosomes | 0.0082 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | – | – | – | – | – | – | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
| Endoplasmic Reticulum | 0.0028 | 0.0061 | 0.0044 | 0.0004 | 0.0034 | 0.0014 | – | – | – | – | – | – | 0.0032 | 0.0027 | 0.0021 | 0.0039 | 0.0024 | 0.0025 |
| Endosome | 0.0078 | 0.0038 | 0.009 | 0.0003 | 0.1181 | 0.0137 | – | – | – | – | – | – | 0.0162 | 0.0024 | 0.0037 | 0.002 | 0.0047 | 0.0041 |
| Golgi | 0.0094 | 0.0007 | 0.007 | 0 | 0.011 | 0.0011 | – | – | – | – | – | – | 0.0081 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0011 |
| Lipid Particles | 0.0602 | 0.0021 | 0.0203 | 0.0001 | 0.0048 | 0.0002 | – | – | – | – | – | – | 0.0207 | 0.0008 | 0.0008 | 0.0074 | 0.0016 | 0.0013 |
| Mitochondria | 0.0845 | 0.0009 | 0.0442 | 0 | 0.0018 | 0.0001 | – | – | – | – | – | – | 0.0076 | 0.0004 | 0.001 | 0.0003 | 0.0011 | 0.002 |
| None | 0.3674 | 0.8192 | 0.6429 | 0.8294 | 0.6681 | 0.6382 | – | – | – | – | – | – | 0.6709 | 0.7088 | 0.748 | 0.8177 | 0.7652 | 0.7429 |
| Nuclear Periphery | 0.0115 | 0.0024 | 0.0029 | 0.0001 | 0.002 | 0.0002 | – | – | – | – | – | – | 0.0092 | 0.0006 | 0.0055 | 0.0011 | 0.0039 | 0.0024 |
| Nucleolus | 0.0066 | 0.0002 | 0.0005 | 0 | 0.0001 | 0 | – | – | – | – | – | – | 0.0017 | 0.0004 | 0.0014 | 0.0016 | 0.0044 | 0.0007 |
| Nucleus | 0.0031 | 0.0009 | 0.0018 | 0.0002 | 0.0006 | 0.0003 | – | – | – | – | – | – | 0.0038 | 0.0015 | 0.0034 | 0.0021 | 0.0078 | 0.0023 |
| Peroxisomes | 0.0319 | 0.0022 | 0.0106 | 0 | 0.0005 | 0.0001 | – | – | – | – | – | – | 0.0156 | 0.0002 | 0.0009 | 0.0005 | 0.0008 | 0.0007 |
| Punctate Nuclear | 0.0079 | 0.0076 | 0.0037 | 0.0001 | 0.0014 | 0.0002 | – | – | – | – | – | – | 0.0144 | 0.0016 | 0.0054 | 0.0064 | 0.0263 | 0.003 |
| Vacuole | 0.0019 | 0.0009 | 0.0016 | 0.0006 | 0.0063 | 0.0003 | – | – | – | – | – | – | 0.0032 | 0.0008 | 0.0007 | 0.0011 | 0.0011 | 0.0013 |
| Vacuole Periphery | 0.0029 | 0.0003 | 0.0004 | 0 | 0.0029 | 0.0001 | – | – | – | – | – | – | 0.0013 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0005 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 1.0589 | 1.2622 | 2.8097 | 2.6321 | 4.1188 | 2.8222 | 3.669 | 3.4453 | 2.5852 | 3.2278 |
| Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | – |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication |
| Localization | |
| Cell Percentages | cytoplasm (15%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | cytoplasm-9 |
Gal3
Gal3
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Gal3-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
