Standard name
Human Ortholog
Description Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functionally complemented by human EIF4A3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0.11 0.18 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.27 0.17 0 0.11 0.15 0.36 0.43 0.25 0.39 0.51 0.54 0.53 0.06 0 0 – – – 0 0 0 0 0 0
Nucleus 0.42 0.64 0.41 0.31 0.61 0.56 0.44 0.45 0.49 0.44 0.46 0.42 0.46 0.44 0.53 – – – 0.21 0.44 0.23 0.36 0.26 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.67 0.47 0.6 0.71 0.3 0.25 0.34 0.34 0.35 0.32 0.37 0.32 0.61 0.47 0.29 – – – 0.63 0.43 0.6 0.46 0.51 0.32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.13 0 0 0 0.06 0.06 0 0 0 0 0.12 0 – – – 0.09 0 0.07 0.06 0.11 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.06 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 0 0
Bud 0 0 0 0 1 1 8 8 6 9 12 10 0 0 0 – – – 1 2 3 1 0 4
Bud Neck 0 0 0 3 0 0 2 2 1 2 2 3 0 0 0 – – – 0 0 0 0 0 1
Bud Site 0 0 0 1 5 3 19 9 13 15 20 26 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 4 17 32 24 43 41 12 15 13 13 4 20 28 – – – 0 0 1 1 0 3
Endoplasmic Reticulum 0 0 2 0 1 0 1 1 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 24 11 9 19 19 35 13 13 1 7 0 5 4 – – – 0 3 14 1 3 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 3 0 0 0
Mitochondria 9 23 16 46 84 230 433 204 250 396 324 409 6 1 1 – – – 0 1 1 0 1 1
Nucleus 14 84 174 135 333 360 438 370 316 335 273 322 43 76 85 – – – 12 63 104 27 33 39
Nuclear Periphery 0 2 9 1 3 7 14 9 11 10 10 9 0 0 0 – – – 0 0 2 0 0 1
Nucleolus 22 62 254 311 163 159 343 280 225 248 221 246 57 82 46 – – – 37 61 273 35 64 48
Peroxisomes 0 0 1 0 0 0 0 0 2 1 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 28 58 6 13 38 48 36 27 19 30 2 21 7 – – – 5 6 33 4 14 13
Vac/Vac Membrane 0 0 4 2 21 28 25 26 24 24 9 16 0 4 7 – – – 2 2 11 2 8 24
Unique Cell Count 33 132 422 436 542 643 1001 815 646 769 597 771 93 174 160 60 143 456 76 127 151
Labelled Cell Count 45 171 516 585 658 844 1383 1033 909 1095 904 1091 112 209 178 60 143 456 76 127 151


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.8 7.0 6.3 5.9 5.0 4.7 4.8 4.6 4.4 4.2 4.3 7.2 8.4 7.8 – – – 8.1 8.7 9.1
Std Deviation (1e-4) 1.0 1.2 1.2 1.4 1.5 1.3 1.4 1.5 1.1 1.1 1.4 0.8 1.2 1.7 1.7 – – – 1.2 1.4 1.5
Intensity Change (Log2) – – – -0.13 -0.25 -0.48 -0.57 -0.53 -0.58 -0.67 -0.73 -0.71 0.05 0.27 0.17 – – – 0.22 0.33 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 2.8 4.0 2.8 3.2 3.6 1.2 1.3 1.5 1.0 0 6.0 7.8 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome -2.3 -3.4 -2.2 0 0 0 0 0 0 0 -1.5 -1.6 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 3.8 5.9 12.1 0 0 0 0 0 0 0 0 0 – – –
Nucleus -3.1 6.2 4.7 0.9 1.4 2.5 0.8 1.4 0.2 0.9 0.6 2.6 – – –
Nuclear Periphery 0 -2.2 -1.4 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 3.4 -9.4 -11.6 0 0 0 0 0 0 0.2 -2.9 -6.8 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 3.3 -4.6 -3.8 0 0 0 0 0 0 -1.7 2.2 -1.0 – – –
Vacuole 0 2.8 3.2 1.9 2.4 2.8 2.4 0.8 1.5 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5045 2.6783 2.1925 1.7938 2.0554 2.3012 2.6654 3.7855 3.1181 2.6095 2.8622 2.9488 1.3588 2.6109 1.9772 1.9435 1.5964 1.8025
Actin 0.0155 0 0.006 0.0001 0.0544 0.0137 0.0333 0.0001 0.0165 0 0.0036 0.0113 0.0014 0 0.0025 0 0 0
Bud 0.0004 0.0001 0.0003 0.0001 0.0002 0.0001 0.0009 0.0001 0.0005 0.0001 0.0015 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0011 0.0008 0.001 0.0013 0.0013 0.0015 0.0024 0.0005 0.001 0.0008 0.0077 0.0009 0.0009 0.0006 0.001 0.0008 0.0008 0.0021
Bud Periphery 0.0005 0.0001 0.0011 0.0003 0.0004 0.0002 0.0016 0.0002 0.0006 0.0002 0.0026 0.0011 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Site 0.0033 0.0006 0.0018 0.0008 0.0008 0.0002 0.0069 0.0025 0.0041 0.0004 0.0141 0.0035 0.0007 0.0003 0.0005 0.0006 0.0003 0.0003
Cell Periphery 0.0002 0.0002 0.0006 0.0003 0.0001 0.0001 0.0008 0.0002 0.0004 0.0002 0.0006 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002
Cytoplasm 0.0064 0.0011 0.0004 0.0009 0.0004 0.0105 0.0149 0.0008 0.0013 0.0001 0.0003 0.0002 0.0025 0 0.0006 0.0003 0 0.0011
Cytoplasmic Foci 0.0104 0.003 0.0037 0.0005 0.0011 0.0003 0.0321 0.0004 0.0057 0.0005 0.0065 0.0069 0.0014 0.0001 0.0007 0.0001 0.0001 0.0047
Eisosomes 0.0002 0 0.0005 0 0.0002 0.0002 0.0002 0 0.0001 0 0.0002 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0033 0.0003 0.0024 0.0001 0.0007 0.0002 0.0053 0.0001 0.0006 0 0.0002 0.0001 0.0019 0 0.0004 0 0 0
Endosome 0.0205 0.0005 0.0038 0.0009 0.0027 0.0009 0.043 0.0003 0.0082 0.0002 0.0038 0.0037 0.0038 0.0001 0.0008 0.0003 0.0001 0.0001
Golgi 0.0056 0 0.0029 0.0002 0.002 0.0015 0.0155 0 0.0055 0.0001 0.003 0.0037 0.0004 0 0.0002 0 0 0
Lipid Particles 0.01 0.0005 0.0077 0.0031 0.0041 0.0015 0.0345 0.0003 0.0164 0.004 0.0095 0.0075 0.0012 0.0003 0.0029 0.0003 0.0002 0.0004
Mitochondria 0.0099 0.0002 0.0102 0.0003 0.0015 0.0011 0.0182 0.0003 0.0064 0.0357 0.0178 0.0028 0.0007 0.0001 0.0003 0.0003 0.0002 0.0004
None 0.0027 0.0007 0.0007 0.0007 0.0007 0.0016 0.0052 0.0002 0.0005 0.0001 0.0005 0.0001 0.0016 0 0.0003 0.0002 0 0.0025
Nuclear Periphery 0.0088 0.0054 0.0095 0.0012 0.0076 0.001 0.0222 0.0006 0.0013 0.0011 0.0037 0.0004 0.0046 0.0001 0.0019 0.0006 0.0006 0.0003
Nucleolus 0.5471 0.6486 0.6794 0.6869 0.6784 0.7176 0.5205 0.702 0.6695 0.6062 0.655 0.7637 0.5972 0.6365 0.6723 0.7129 0.6175 0.6999
Nucleus 0.3203 0.3098 0.2501 0.2936 0.2292 0.2373 0.1875 0.28 0.2457 0.3454 0.2395 0.1666 0.3385 0.3594 0.3101 0.2792 0.3454 0.2676
Peroxisomes 0.0039 0.0001 0.0097 0.0002 0.0007 0.0003 0.0129 0 0.0039 0.0002 0.0138 0.008 0.0002 0 0.0001 0 0 0
Punctate Nuclear 0.0267 0.0268 0.0048 0.0066 0.012 0.01 0.0226 0.008 0.004 0.0034 0.0113 0.0177 0.0411 0.0012 0.0036 0.0027 0.0342 0.0197
Vacuole 0.0018 0.0011 0.0016 0.0019 0.0008 0.0002 0.0137 0.0024 0.0058 0.0009 0.0032 0.0007 0.001 0.0008 0.0008 0.0009 0.0002 0.0004
Vacuole Periphery 0.0013 0.0002 0.0017 0.0003 0.0006 0.0002 0.0057 0.0009 0.002 0.0003 0.0015 0.0002 0.0005 0.0001 0.0002 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.1486 10.6857 8.5392 9.5001 14.3061 9.1899 9.1421 8.4141 6.2953 12.7102
Translational Efficiency 1.076 1.9636 1.887 1.0132 1.2987 1.2859 1.3207 0.8506 0.8808 1.1838

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
615 205 234 1216 423 1572 466 1642 1038 1777 700 2858

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 828.84 972.59 1114.26 1116.76 810.48 920.80 1117.17 1126.69 821.36 926.77 1116.20 1122.47
Standard Deviation 126.58 152.30 132.10 196.05 122.80 138.78 158.40 194.17 125.38 141.38 150.13 195.04
Intensity Change Log 2 — 0.230738 0.426920 0.430154 — 0.184111 0.463000 0.475242 — 0.207874 0.444871 0.452622

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000335 0.000820 0.001034 0.000966 0.000713 0.000579 0.000556 0.000741 0.000489 0.000607 0.000716 0.000837
Bud Neck 0.004116 0.014015 0.012505 0.031636 0.005947 0.007750 0.013038 0.038691 0.004862 0.008472 0.012860 0.035689
Bud Site 0.000682 0.001794 0.001737 0.004395 0.001814 0.001251 0.001221 0.004016 0.001143 0.001314 0.001393 0.004177
Cell Periphery 0.000055 0.000078 0.000039 0.000144 0.000110 0.000068 0.000019 0.000107 0.000077 0.000069 0.000026 0.000122
Cytoplasm 0.001791 0.001296 0.000190 0.001309 0.007392 0.001067 0.000073 0.003792 0.004074 0.001093 0.000112 0.002735
Cytoplasmic Foci 0.000459 0.000366 0.000086 0.001630 0.001544 0.001759 0.000043 0.000894 0.000901 0.001599 0.000057 0.001207
Eisosomes 0.000044 0.000049 0.000063 0.000047 0.000044 0.000047 0.000043 0.000048 0.000044 0.000047 0.000049 0.000048
Endoplasmic Reticulum 0.001697 0.002789 0.003698 0.001995 0.003439 0.002293 0.001486 0.002042 0.002407 0.002350 0.002225 0.002022
Endosome 0.000465 0.000503 0.000331 0.002994 0.001086 0.002456 0.000177 0.002093 0.000718 0.002231 0.000229 0.002476
Golgi 0.000383 0.000489 0.000364 0.003509 0.000652 0.001447 0.000302 0.000917 0.000493 0.001337 0.000323 0.002020
Lipid Particles 0.000375 0.000579 0.000197 0.000639 0.000380 0.001745 0.000062 0.000178 0.000377 0.001610 0.000107 0.000374
Mitochondria 0.000770 0.001651 0.001058 0.005466 0.000849 0.003258 0.000771 0.004061 0.000802 0.003073 0.000867 0.004659
Mitotic Spindle 0.005779 0.012963 0.016386 0.018306 0.001211 0.014300 0.002835 0.017208 0.003917 0.014145 0.007365 0.017675
None 0.001704 0.002117 0.000653 0.002267 0.010414 0.002177 0.000695 0.002345 0.005254 0.002170 0.000681 0.002312
Nuclear Periphery 0.000290 0.000410 0.000371 0.003137 0.000308 0.000902 0.000348 0.001705 0.000298 0.000845 0.000356 0.002315
Nuclear Periphery Foci 0.006528 0.002315 0.001357 0.001423 0.003861 0.003942 0.000810 0.001192 0.005441 0.003754 0.000993 0.001291
Nucleolus 0.492610* 0.432696* 0.492454* 0.433409* 0.322693* 0.442561* 0.538402* 0.358999* 0.423366* 0.441423* 0.523042* 0.390659*
Nucleus 0.479362* 0.521261* 0.460993* 0.473465* 0.634425* 0.507001* 0.435312* 0.550673* 0.542553* 0.508646* 0.443897* 0.517823*
Peroxisomes 0.001952 0.002291 0.004214 0.001837 0.001765 0.002531 0.002685 0.001405 0.001876 0.002503 0.003196 0.001589
Vacuole 0.000332 0.000923 0.001836 0.007443 0.000938 0.001928 0.000767 0.005532 0.000579 0.001812 0.001124 0.006345
Vacuole Periphery 0.000268 0.000595 0.000433 0.003985 0.000412 0.000940 0.000356 0.003361 0.000327 0.000900 0.000382 0.003626

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.98 -8.08 -10.28 -1.26 1.31 1.28 0.47 -0.89 -2.92 -2.70 -1.25 -5.67 -7.79 -4.06 -1.16
Bud Neck -2.87 -6.22 -17.92 -5.96 -8.44 -1.20 -6.72 -20.38 -20.74 -13.30 -4.25 -9.93 -27.75 -23.87 -15.65
Bud Site -2.65 -5.59 -6.06 -3.65 -3.66 1.43 0.19 -3.56 -7.73 -5.75 -0.34 -2.91 -7.80 -8.38 -6.49
Cell Periphery -0.79 0.33 -3.08 -1.77 -3.78 2.03 4.34 0.40 -2.17 -6.01 0.61 3.54 -2.38 -3.13 -6.77
Cytoplasm 0.54 1.64 -0.10 -0.93 -3.39 3.05 3.52 1.56 -4.60 -6.43 2.89 3.85 0.86 -4.47 -7.28
Cytoplasmic Foci 0.41 2.18 -1.94 -2.20 -3.14 0.31 2.54 1.71 2.25 -2.99 -0.92 3.12 0.49 1.52 -4.32
Eisosomes -1.38 -5.03 -0.59 1.02 4.71 -0.43 0.21 -0.20 0.32 -0.56 -1.25 -3.10 -0.68 0.73 2.82
Endoplasmic Reticulum -2.95 -4.78 -2.47 1.72 3.84 3.80 6.44 4.13 0.23 -3.70 0.07 -0.31 1.08 0.95 1.20
Endosome -0.33 0.26 -4.01 -3.97 -4.26 -2.11 3.24 -0.95 1.39 -4.23 -3.03 3.13 -3.80 0.62 -6.07
Golgi -1.08 -0.87 -2.49 -2.33 -2.43 -1.76 2.60 -0.03 1.60 -1.88 -2.54 2.05 -2.51 -0.59 -2.90
Lipid Particles -0.59 1.26 -0.47 0.09 -0.88 -2.60 4.34 2.72 3.08 -2.96 -2.68 3.80 0.33 2.69 -1.39
Mitochondria -2.14 -2.89 -5.09 -3.47 -4.49 -3.20 -1.41 -4.93 -0.78 -4.69 -3.41 -2.59 -7.07 -1.75 -6.55
Mitotic Spindle -1.69 -2.10 -4.73 -0.74 -0.19 -5.45 -2.14 -7.06 -1.55 -4.81 -4.37 -2.30 -7.47 -2.20 -3.11
None -0.56 2.45 -1.16 -0.34 -3.34 3.71 4.41 3.59 -0.75 -6.06 2.97 4.79 2.88 -0.65 -6.34
Nuclear Periphery -1.79 -2.60 -4.80 -4.36 -4.45 -3.50 -0.57 -6.15 -3.00 -5.57 -3.83 -1.54 -7.17 -4.60 -6.63
Nuclear Periphery Foci 2.84 3.91 3.93 1.52 0.17 0.71 3.96 3.83 3.98 -0.43 1.93 5.55 5.41 3.91 -0.43
Nucleolus 3.42 1.28 6.93 1.42 3.18 -8.12 -10.89 -0.39 11.66 13.36 -1.51 -5.80 6.38 9.71 12.07
Nucleus -2.08 0.32 -0.58 1.73 -0.70 8.25 10.32 4.34 -5.91 -8.60 3.11 6.63 0.96 -2.74 -6.73
Peroxisomes -1.15 -5.44 0.32 1.55 6.21 -1.17 -2.17 1.76 2.59 6.00 -1.40 -5.10 1.86 2.37 8.31
Vacuole -2.60 -6.73 -5.70 -5.05 -4.07 -1.57 -0.10 -5.28 -3.99 -5.34 -2.70 -4.49 -7.99 -5.60 -6.78
Vacuole Periphery -2.15 -3.61 -4.67 -4.05 -4.33 -2.75 0.33 -4.84 -3.59 -4.94 -3.57 -2.11 -6.79 -5.04 -6.57
Standard name
Human Ortholog
Description Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functionally complemented by human EIF4A3
Localization
Cell Percentages nucleolus (47%), nucleus (33%), mixed (19%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fal1

Fal1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fal1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available