Standard name | |
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Human Ortholog | |
Description | Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functionally complemented by human EIF4A3 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 1 | 8 | 8 | 6 | 9 | 12 | 10 | 0 | 0 | 0 | – | – | – | 1 | 2 | 3 | 1 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 2 | 1 | 2 | 2 | 3 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 5 | 3 | 19 | 9 | 13 | 15 | 20 | 26 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 4 | 17 | 32 | 24 | 43 | 41 | 12 | 15 | 13 | 13 | 4 | 20 | 28 | – | – | – | 0 | 0 | 1 | 1 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 24 | 11 | 9 | 19 | 19 | 35 | 13 | 13 | 1 | 7 | 0 | 5 | 4 | – | – | – | 0 | 3 | 14 | 1 | 3 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 3 | 0 | 0 | 0 |
Mitochondria | 9 | 23 | 16 | 46 | 84 | 230 | 433 | 204 | 250 | 396 | 324 | 409 | 6 | 1 | 1 | – | – | – | 0 | 1 | 1 | 0 | 1 | 1 |
Nucleus | 14 | 84 | 174 | 135 | 333 | 360 | 438 | 370 | 316 | 335 | 273 | 322 | 43 | 76 | 85 | – | – | – | 12 | 63 | 104 | 27 | 33 | 39 |
Nuclear Periphery | 0 | 2 | 9 | 1 | 3 | 7 | 14 | 9 | 11 | 10 | 10 | 9 | 0 | 0 | 0 | – | – | – | 0 | 0 | 2 | 0 | 0 | 1 |
Nucleolus | 22 | 62 | 254 | 311 | 163 | 159 | 343 | 280 | 225 | 248 | 221 | 246 | 57 | 82 | 46 | – | – | – | 37 | 61 | 273 | 35 | 64 | 48 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 28 | 58 | 6 | 13 | 38 | 48 | 36 | 27 | 19 | 30 | 2 | 21 | 7 | – | – | – | 5 | 6 | 33 | 4 | 14 | 13 |
Vac/Vac Membrane | 0 | 0 | 4 | 2 | 21 | 28 | 25 | 26 | 24 | 24 | 9 | 16 | 0 | 4 | 7 | – | – | – | 2 | 2 | 11 | 2 | 8 | 24 |
Unique Cell Count | 33 | 132 | 422 | 436 | 542 | 643 | 1001 | 815 | 646 | 769 | 597 | 771 | 93 | 174 | 160 | 60 | 143 | 456 | 76 | 127 | 151 | |||
Labelled Cell Count | 45 | 171 | 516 | 585 | 658 | 844 | 1383 | 1033 | 909 | 1095 | 904 | 1091 | 112 | 209 | 178 | 60 | 143 | 456 | 76 | 127 | 151 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.8 | 7.0 | 6.3 | 5.9 | 5.0 | 4.7 | 4.8 | 4.6 | 4.4 | 4.2 | 4.3 | 7.2 | 8.4 | 7.8 | – | – | – | 8.1 | 8.7 | 9.1 |
Std Deviation (1e-4) | 1.0 | 1.2 | 1.2 | 1.4 | 1.5 | 1.3 | 1.4 | 1.5 | 1.1 | 1.1 | 1.4 | 0.8 | 1.2 | 1.7 | 1.7 | – | – | – | 1.2 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.25 | -0.48 | -0.57 | -0.53 | -0.58 | -0.67 | -0.73 | -0.71 | 0.05 | 0.27 | 0.17 | – | – | – | 0.22 | 0.33 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 2.8 | 4.0 | 2.8 | 3.2 | 3.6 | 1.2 | 1.3 | 1.5 | 1.0 | 0 | 6.0 | 7.8 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Endosome | -2.3 | -3.4 | -2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | -1.6 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 3.8 | 5.9 | 12.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | -3.1 | 6.2 | 4.7 | 0.9 | 1.4 | 2.5 | 0.8 | 1.4 | 0.2 | 0.9 | 0.6 | 2.6 | – | – | – |
Nuclear Periphery | 0 | -2.2 | -1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 3.4 | -9.4 | -11.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | -2.9 | -6.8 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 3.3 | -4.6 | -3.8 | 0 | 0 | 0 | 0 | 0 | 0 | -1.7 | 2.2 | -1.0 | – | – | – |
Vacuole | 0 | 2.8 | 3.2 | 1.9 | 2.4 | 2.8 | 2.4 | 0.8 | 1.5 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5045 | 2.6783 | 2.1925 | 1.7938 | 2.0554 | 2.3012 | 2.6654 | 3.7855 | 3.1181 | 2.6095 | 2.8622 | 2.9488 | 1.3588 | 2.6109 | 1.9772 | 1.9435 | 1.5964 | 1.8025 |
Actin | 0.0155 | 0 | 0.006 | 0.0001 | 0.0544 | 0.0137 | 0.0333 | 0.0001 | 0.0165 | 0 | 0.0036 | 0.0113 | 0.0014 | 0 | 0.0025 | 0 | 0 | 0 |
Bud | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0001 | 0.0015 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0011 | 0.0008 | 0.001 | 0.0013 | 0.0013 | 0.0015 | 0.0024 | 0.0005 | 0.001 | 0.0008 | 0.0077 | 0.0009 | 0.0009 | 0.0006 | 0.001 | 0.0008 | 0.0008 | 0.0021 |
Bud Periphery | 0.0005 | 0.0001 | 0.0011 | 0.0003 | 0.0004 | 0.0002 | 0.0016 | 0.0002 | 0.0006 | 0.0002 | 0.0026 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0033 | 0.0006 | 0.0018 | 0.0008 | 0.0008 | 0.0002 | 0.0069 | 0.0025 | 0.0041 | 0.0004 | 0.0141 | 0.0035 | 0.0007 | 0.0003 | 0.0005 | 0.0006 | 0.0003 | 0.0003 |
Cell Periphery | 0.0002 | 0.0002 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.0064 | 0.0011 | 0.0004 | 0.0009 | 0.0004 | 0.0105 | 0.0149 | 0.0008 | 0.0013 | 0.0001 | 0.0003 | 0.0002 | 0.0025 | 0 | 0.0006 | 0.0003 | 0 | 0.0011 |
Cytoplasmic Foci | 0.0104 | 0.003 | 0.0037 | 0.0005 | 0.0011 | 0.0003 | 0.0321 | 0.0004 | 0.0057 | 0.0005 | 0.0065 | 0.0069 | 0.0014 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0047 |
Eisosomes | 0.0002 | 0 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0033 | 0.0003 | 0.0024 | 0.0001 | 0.0007 | 0.0002 | 0.0053 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0019 | 0 | 0.0004 | 0 | 0 | 0 |
Endosome | 0.0205 | 0.0005 | 0.0038 | 0.0009 | 0.0027 | 0.0009 | 0.043 | 0.0003 | 0.0082 | 0.0002 | 0.0038 | 0.0037 | 0.0038 | 0.0001 | 0.0008 | 0.0003 | 0.0001 | 0.0001 |
Golgi | 0.0056 | 0 | 0.0029 | 0.0002 | 0.002 | 0.0015 | 0.0155 | 0 | 0.0055 | 0.0001 | 0.003 | 0.0037 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 |
Lipid Particles | 0.01 | 0.0005 | 0.0077 | 0.0031 | 0.0041 | 0.0015 | 0.0345 | 0.0003 | 0.0164 | 0.004 | 0.0095 | 0.0075 | 0.0012 | 0.0003 | 0.0029 | 0.0003 | 0.0002 | 0.0004 |
Mitochondria | 0.0099 | 0.0002 | 0.0102 | 0.0003 | 0.0015 | 0.0011 | 0.0182 | 0.0003 | 0.0064 | 0.0357 | 0.0178 | 0.0028 | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 |
None | 0.0027 | 0.0007 | 0.0007 | 0.0007 | 0.0007 | 0.0016 | 0.0052 | 0.0002 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0016 | 0 | 0.0003 | 0.0002 | 0 | 0.0025 |
Nuclear Periphery | 0.0088 | 0.0054 | 0.0095 | 0.0012 | 0.0076 | 0.001 | 0.0222 | 0.0006 | 0.0013 | 0.0011 | 0.0037 | 0.0004 | 0.0046 | 0.0001 | 0.0019 | 0.0006 | 0.0006 | 0.0003 |
Nucleolus | 0.5471 | 0.6486 | 0.6794 | 0.6869 | 0.6784 | 0.7176 | 0.5205 | 0.702 | 0.6695 | 0.6062 | 0.655 | 0.7637 | 0.5972 | 0.6365 | 0.6723 | 0.7129 | 0.6175 | 0.6999 |
Nucleus | 0.3203 | 0.3098 | 0.2501 | 0.2936 | 0.2292 | 0.2373 | 0.1875 | 0.28 | 0.2457 | 0.3454 | 0.2395 | 0.1666 | 0.3385 | 0.3594 | 0.3101 | 0.2792 | 0.3454 | 0.2676 |
Peroxisomes | 0.0039 | 0.0001 | 0.0097 | 0.0002 | 0.0007 | 0.0003 | 0.0129 | 0 | 0.0039 | 0.0002 | 0.0138 | 0.008 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.0267 | 0.0268 | 0.0048 | 0.0066 | 0.012 | 0.01 | 0.0226 | 0.008 | 0.004 | 0.0034 | 0.0113 | 0.0177 | 0.0411 | 0.0012 | 0.0036 | 0.0027 | 0.0342 | 0.0197 |
Vacuole | 0.0018 | 0.0011 | 0.0016 | 0.0019 | 0.0008 | 0.0002 | 0.0137 | 0.0024 | 0.0058 | 0.0009 | 0.0032 | 0.0007 | 0.001 | 0.0008 | 0.0008 | 0.0009 | 0.0002 | 0.0004 |
Vacuole Periphery | 0.0013 | 0.0002 | 0.0017 | 0.0003 | 0.0006 | 0.0002 | 0.0057 | 0.0009 | 0.002 | 0.0003 | 0.0015 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.1486 | 10.6857 | 8.5392 | 9.5001 | 14.3061 | 9.1899 | 9.1421 | 8.4141 | 6.2953 | 12.7102 |
Translational Efficiency | 1.076 | 1.9636 | 1.887 | 1.0132 | 1.2987 | 1.2859 | 1.3207 | 0.8506 | 0.8808 | 1.1838 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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