Standard name | |
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Human Ortholog | |
Description | Autophagy-specific protein required for autophagosome formation; forms a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; constitutively phosphorylated, and phosphorylation of residue S174 is required for function; high-copy suppressor of CIK1 deletion |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 3 | 0 | 4 | 0 | 4 | 0 | 0 | 0 | 2 | 1 | 2 | 4 | 2 | 2 | 1 | 4 | 2 |
Bud | 1 | 1 | 0 | 1 | 5 | 9 | 5 | 18 | 9 | 23 | 17 | 16 | 2 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 6 | 6 |
Bud Neck | 0 | 2 | 0 | 8 | 2 | 9 | 8 | 21 | 6 | 10 | 0 | 5 | 2 | 2 | 1 | 1 | 2 | 0 | 1 | 2 | 1 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 3 | 5 | 1 | 4 | 0 | 1 | 0 | 0 | 3 | 2 | 0 | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 3 | 0 | 1 |
Cytoplasm | 225 | 189 | 153 | 213 | 202 | 214 | 249 | 313 | 200 | 216 | 140 | 237 | 205 | 341 | 336 | 128 | 73 | 65 | 177 | 173 | 126 | 173 | 278 | 207 |
Endoplasmic Reticulum | 0 | 1 | 1 | 1 | 0 | 3 | 0 | 2 | 5 | 1 | 1 | 4 | 1 | 0 | 1 | 15 | 1 | 2 | 0 | 0 | 1 | 0 | 5 | 1 |
Endosome | 3 | 0 | 0 | 1 | 17 | 5 | 38 | 27 | 28 | 33 | 26 | 20 | 4 | 4 | 17 | 5 | 2 | 3 | 11 | 3 | 2 | 2 | 11 | 6 |
Golgi | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 10 | 5 | 0 | 0 | 0 | 48 | 13 | 43 | 59 | 76 | 93 | 137 | 1 | 1 | 1 | 10 | 1 | 4 | 36 | 11 | 18 | 28 | 27 | 19 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 3 | 2 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 7 | 8 | 5 | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 3 | 0 | 1 | 18 | 38 | 35 | 91 | 77 | 49 | 76 | 30 | 36 | 3 | 5 | 16 | 2 | 2 | 1 | 4 | 0 | 3 | 4 | 7 | 6 |
Vac/Vac Membrane | 1 | 0 | 0 | 1 | 3 | 4 | 9 | 9 | 5 | 11 | 4 | 8 | 1 | 0 | 0 | 5 | 0 | 1 | 3 | 2 | 1 | 3 | 8 | 5 |
Unique Cell Count | 243 | 193 | 155 | 237 | 254 | 297 | 366 | 451 | 321 | 371 | 260 | 400 | 216 | 352 | 364 | 150 | 82 | 72 | 251 | 203 | 167 | 222 | 364 | 266 |
Labelled Cell Count | 247 | 199 | 158 | 251 | 271 | 335 | 420 | 524 | 372 | 463 | 319 | 474 | 221 | 355 | 377 | 173 | 87 | 80 | 251 | 203 | 167 | 222 | 364 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 3.9 | 5.0 | 5.0 | 5.4 | 3.7 | 4.5 | 4.3 | 4.2 | 4.2 | 3.9 | 3.9 | 4.3 | 4.6 | 4.8 | 5.6 | 5.6 | 5.6 | 3.8 | 4.2 | 4.3 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.7 | 2.9 | 1.8 | 0.9 | 1.3 | 1.2 | 1.9 | 1.4 | 0.7 | 0.9 | 1.2 | 1.5 | 1.5 | 1.5 | 1.6 | 1.6 | 0.8 | 1.7 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.11 | -0.44 | -0.16 | -0.23 | -0.27 | -0.25 | -0.36 | -0.38 | -0.22 | -0.12 | -0.07 | 0.15 | 0.16 | 0.16 | -0.41 | -0.27 | -0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6166 | -1.0803 | -1.0724 | -1.3319 | -0.6298 | -1.1572 | -0.197 | 0.1708 | 0.0861 | -0.1145 | 0.0019 | -0.0116 | 1.5356 | 2.158 | 1.9279 | 1.1851 | 1.0472 | 1.7367 |
Actin | 0.0676 | 0.0001 | 0.0025 | 0 | 0.0459 | 0.0008 | 0.0244 | 0.0015 | 0.0133 | 0.0087 | 0.0155 | 0.0123 | 0.0791 | 0.0015 | 0.0168 | 0.0344 | 0.0125 | 0.0004 |
Bud | 0.0009 | 0.0001 | 0.0076 | 0 | 0.0015 | 0.0002 | 0.0012 | 0.0112 | 0.001 | 0.0031 | 0.0036 | 0.0007 | 0.0013 | 0.0009 | 0.003 | 0.0003 | 0.0003 | 0.0001 |
Bud Neck | 0.0037 | 0 | 0.0003 | 0 | 0.0011 | 0.0001 | 0.0044 | 0.0004 | 0.0015 | 0.0004 | 0.0007 | 0.0013 | 0.0019 | 0.0019 | 0.0028 | 0.0431 | 0.0002 | 0.0005 |
Bud Periphery | 0.0011 | 0.0001 | 0.0077 | 0 | 0.0024 | 0.0006 | 0.0027 | 0.0048 | 0.0014 | 0.0077 | 0.0084 | 0.0028 | 0.0037 | 0.0019 | 0.0022 | 0.001 | 0.0004 | 0.0004 |
Bud Site | 0.0055 | 0.0006 | 0.002 | 0 | 0.0014 | 0.0003 | 0.0147 | 0.02 | 0.0016 | 0.0024 | 0.005 | 0.0003 | 0.0036 | 0.0365 | 0.003 | 0.0009 | 0.0002 | 0.0001 |
Cell Periphery | 0.0003 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.001 | 0.0003 | 0.0005 | 0 | 0.0001 |
Cytoplasm | 0.0329 | 0.057 | 0.0279 | 0.0131 | 0.0244 | 0.0558 | 0.0895 | 0.2121 | 0.1598 | 0.1254 | 0.155 | 0.2079 | 0.0466 | 0.0796 | 0.0661 | 0.0778 | 0.112 | 0.0995 |
Cytoplasmic Foci | 0.0312 | 0.0197 | 0.0127 | 0.0039 | 0.005 | 0.011 | 0.0267 | 0.0238 | 0.0178 | 0.0327 | 0.0179 | 0.0321 | 0.0464 | 0.0047 | 0.0218 | 0.0197 | 0.0362 | 0.0207 |
Eisosomes | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0009 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0045 | 0.0005 | 0.0006 | 0.0004 | 0.0025 | 0.0015 | 0.008 | 0.0023 | 0.0018 | 0.0013 | 0.0209 | 0.0013 | 0.0054 | 0.0011 | 0.0011 | 0.0005 | 0.001 | 0.0005 |
Endosome | 0.04 | 0.0039 | 0.0075 | 0.0016 | 0.0081 | 0.0064 | 0.0252 | 0.0077 | 0.0169 | 0.0203 | 0.04 | 0.0096 | 0.0148 | 0.0004 | 0.0039 | 0.0013 | 0.0086 | 0.0045 |
Golgi | 0.0108 | 0.0002 | 0.0006 | 0 | 0.0048 | 0.0011 | 0.0052 | 0.0004 | 0.0024 | 0.0028 | 0.021 | 0.0017 | 0.0089 | 0.0001 | 0.0019 | 0.002 | 0.0018 | 0.0006 |
Lipid Particles | 0.0156 | 0.0014 | 0.0007 | 0.002 | 0.003 | 0.0017 | 0.0059 | 0.0007 | 0.0046 | 0.0057 | 0.0285 | 0.0034 | 0.0239 | 0.0003 | 0.013 | 0.0032 | 0.0086 | 0.0052 |
Mitochondria | 0.0051 | 0.0002 | 0.0036 | 0.0001 | 0.0019 | 0.0036 | 0.0084 | 0.0006 | 0.001 | 0.0444 | 0.0238 | 0.0027 | 0.0033 | 0.0003 | 0.0013 | 0.0007 | 0.0009 | 0.0046 |
None | 0.7354 | 0.9144 | 0.9163 | 0.97 | 0.8844 | 0.9124 | 0.7547 | 0.7075 | 0.7661 | 0.73 | 0.6066 | 0.711 | 0.7189 | 0.8658 | 0.8449 | 0.7991 | 0.7956 | 0.8603 |
Nuclear Periphery | 0.0094 | 0.0002 | 0.0021 | 0.0034 | 0.0066 | 0.0008 | 0.0098 | 0.0008 | 0.0015 | 0.001 | 0.0251 | 0.005 | 0.0111 | 0.0003 | 0.0016 | 0.0003 | 0.0017 | 0.0001 |
Nucleolus | 0.0007 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0009 | 0.0004 | 0.0002 | 0.0008 | 0.0017 | 0.0002 | 0.0006 | 0.0003 | 0.0008 | 0.0003 | 0.0004 | 0.0001 |
Nucleus | 0.0039 | 0.0002 | 0.0035 | 0.0009 | 0.001 | 0.0007 | 0.0026 | 0.0015 | 0.0031 | 0.0023 | 0.0074 | 0.0014 | 0.0052 | 0.0008 | 0.0011 | 0.0005 | 0.0006 | 0.0002 |
Peroxisomes | 0.0141 | 0.0003 | 0.0003 | 0.0001 | 0.0011 | 0.0008 | 0.0045 | 0.0004 | 0.0025 | 0.0067 | 0.0043 | 0.0013 | 0.0165 | 0.0002 | 0.0081 | 0.0112 | 0.0093 | 0.0016 |
Punctate Nuclear | 0.0116 | 0.0004 | 0.001 | 0.0036 | 0.0032 | 0.0011 | 0.0043 | 0.0016 | 0.0012 | 0.0016 | 0.006 | 0.0031 | 0.0033 | 0.0012 | 0.0051 | 0.0021 | 0.0086 | 0.0005 |
Vacuole | 0.0037 | 0.0005 | 0.0015 | 0.0003 | 0.0007 | 0.0006 | 0.0033 | 0.0017 | 0.0017 | 0.0015 | 0.004 | 0.001 | 0.003 | 0.0004 | 0.0007 | 0.0006 | 0.0006 | 0.0002 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0021 | 0.0002 | 0.0004 | 0.0007 | 0.0036 | 0.0005 | 0.0012 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.6675 | 10.014 | 11.1462 | 10.0641 | 3.631 | 9.3299 | 8.0157 | 12.5286 | 14.8632 | 13.2015 |
Translational Efficiency | 1.5445 | 0.8586 | 0.6122 | 0.7151 | 2.2245 | 1.2938 | 1.8046 | 1.1347 | 1.035 | 1.3827 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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