Standard name | |
---|---|
Human Ortholog | |
Description | Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 10 | 9 | 2 | 8 | 10 | 22 | 25 | 16 | 11 | 12 | 13 | 2 | 2 | 2 | 9 | 1 | 9 | 1 | 7 | 5 | 8 | 6 | 11 |
Bud | 2 | 2 | 1 | 2 | 1 | 4 | 13 | 12 | 12 | 17 | 12 | 12 | 1 | 1 | 0 | 2 | 0 | 1 | 4 | 12 | 2 | 2 | 2 | 7 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 1 | 0 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 9 | 11 | 8 | 16 | 23 | 28 | 24 | 24 | 28 | 19 | 39 | 2 | 27 | 19 | 22 | 9 | 24 | 0 | 1 | 0 | 6 | 4 | 9 |
Cytoplasm | 53 | 182 | 43 | 53 | 78 | 83 | 89 | 111 | 123 | 101 | 119 | 130 | 28 | 106 | 175 | 28 | 20 | 41 | 18 | 82 | 3 | 16 | 30 | 57 |
Endoplasmic Reticulum | 45 | 125 | 51 | 49 | 73 | 146 | 121 | 121 | 76 | 90 | 76 | 90 | 28 | 44 | 56 | 0 | 1 | 4 | 86 | 185 | 98 | 114 | 114 | 124 |
Endosome | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 3 |
Golgi | 0 | 1 | 1 | 2 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 8 | 9 | 15 | 0 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 6 | 3 | 2 | 11 | 9 | 23 | 49 | 68 | 45 | 57 | 55 | 48 | 0 | 0 | 0 | 4 | 0 | 1 | 5 | 11 | 1 | 32 | 16 | 26 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 2 | 0 | 2 | 3 | 5 | 3 | 3 | 6 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 4 | 11 | 4 | 7 | 19 | 17 | 8 | 15 | 15 | 17 | 15 | 19 | 4 | 1 | 3 | 6 | 2 | 6 | 1 | 18 | 1 | 5 | 8 | 15 |
Unique Cell Count | 102 | 311 | 99 | 110 | 168 | 251 | 256 | 301 | 256 | 257 | 251 | 290 | 61 | 162 | 239 | 66 | 39 | 86 | 124 | 336 | 116 | 193 | 188 | 264 |
Labelled Cell Count | 115 | 346 | 124 | 136 | 215 | 309 | 335 | 382 | 319 | 326 | 318 | 359 | 65 | 181 | 260 | 79 | 42 | 101 | 124 | 336 | 116 | 193 | 188 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 6.8 | 8.3 | 8.6 | 10.8 | 10.2 | 9.5 | 9.4 | 9.4 | 8.7 | 8.6 | 8.4 | 7.8 | 6.9 | 6.5 | 28.6 | 26.9 | 26.8 | 7.3 | 7.3 | 7.1 |
Std Deviation (1e-4) | 2.1 | 2.0 | 3.8 | 3.4 | 4.3 | 3.9 | 4.2 | 3.7 | 4.1 | 3.7 | 4.0 | 3.4 | 2.3 | 1.6 | 1.5 | 15.3 | 16.5 | 15.0 | 1.9 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.38 | 0.3 | 0.2 | 0.17 | 0.19 | 0.06 | 0.04 | 0.02 | -0.09 | -0.26 | -0.34 | 1.78 | 1.7 | 1.69 | -0.18 | -0.18 | -0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.4 | -1.4 | -1.9 | -0.1 | -0.2 | -0.9 | -1.8 | -1.5 | -1.7 | 0 | 0 | 0 | 0.9 | 0 | 0.3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -0.4 | -0.6 | 0 | -1.0 | -0.5 | -0.1 | -1.1 | 0.6 | 0 | 1.2 | -0.9 | 3.5 | 1.8 | 2.9 |
Cytoplasm | 0.7 | 0.5 | -1.8 | -1.5 | -1.2 | 0.8 | -0.7 | 0.7 | 0.2 | 0.3 | 3.5 | 5.2 | -0.1 | 0.8 | 0.6 |
Endoplasmic Reticulum | -1.0 | -1.3 | 1.1 | -0.7 | -2.0 | -3.8 | -2.9 | -3.7 | -3.7 | -0.7 | -4.0 | -5.1 | -7.0 | -5.3 | -7.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 |
Mitochondria | 2.4 | 0 | 2.3 | 4.1 | 4.7 | 3.9 | 4.6 | 4.5 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.8 | 2.0 | 1.0 | 0 | 0 | 0.7 | 0.9 | 0.7 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.397 | 6.0507 | 3.4273 | 2.0699 | 2.7023 | 4.4595 | 4.962 | 5.958 | 4.5779 | 2.6065 | 3.3816 | 5.1887 | 4.5161 | 5.4762 | 2.7718 | 2.9405 | 1.2139 | 3.7754 |
Actin | 0.0186 | 0.0048 | 0.0258 | 0.0077 | 0.0569 | 0.0071 | 0.0337 | 0.0016 | 0.0056 | 0.0082 | 0.0059 | 0.0118 | 0.0111 | 0.0005 | 0.0197 | 0.0022 | 0.0646 | 0.0143 |
Bud | 0.0002 | 0.0015 | 0.0007 | 0.0016 | 0.0008 | 0.0008 | 0.0006 | 0.0008 | 0.0006 | 0.0021 | 0.0006 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0029 | 0.0002 |
Bud Neck | 0.0004 | 0.0006 | 0.0013 | 0.0007 | 0.0025 | 0.0039 | 0.0045 | 0.0005 | 0.0009 | 0.0019 | 0.001 | 0.0018 | 0.0002 | 0.0004 | 0.0006 | 0.037 | 0.0017 | 0.0056 |
Bud Periphery | 0.0003 | 0.0078 | 0.002 | 0.0056 | 0.0016 | 0.0019 | 0.0017 | 0.0025 | 0.0015 | 0.0154 | 0.0008 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0214 | 0.0006 |
Bud Site | 0.0015 | 0.0149 | 0.0113 | 0.0016 | 0.0014 | 0.0003 | 0.0052 | 0.0199 | 0.0074 | 0.0063 | 0.0017 | 0.0002 | 0.0004 | 0.0006 | 0.0012 | 0.0026 | 0.0025 | 0.0005 |
Cell Periphery | 0.0023 | 0.0075 | 0.0055 | 0.0048 | 0.0017 | 0.0026 | 0.0029 | 0.01 | 0.0033 | 0.005 | 0.0012 | 0.0016 | 0.0014 | 0.0135 | 0.0023 | 0.0087 | 0.0078 | 0.0021 |
Cytoplasm | 0.144 | 0.1697 | 0.2206 | 0.381 | 0.3579 | 0.1718 | 0.1528 | 0.1737 | 0.2613 | 0.1915 | 0.3378 | 0.1898 | 0.1593 | 0.1929 | 0.2872 | 0.2196 | 0.3055 | 0.2171 |
Cytoplasmic Foci | 0.0128 | 0.0039 | 0.014 | 0.0083 | 0.0105 | 0.0052 | 0.0162 | 0.0073 | 0.0191 | 0.01 | 0.0215 | 0.008 | 0.0069 | 0.0026 | 0.0086 | 0.0058 | 0.0064 | 0.0042 |
Eisosomes | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0018 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 |
Endoplasmic Reticulum | 0.7333 | 0.7361 | 0.5908 | 0.4073 | 0.3914 | 0.7558 | 0.6644 | 0.7202 | 0.5607 | 0.4666 | 0.3849 | 0.6944 | 0.7245 | 0.7054 | 0.5088 | 0.5594 | 0.2839 | 0.5968 |
Endosome | 0.0109 | 0.0046 | 0.0159 | 0.0141 | 0.036 | 0.0092 | 0.0185 | 0.007 | 0.0199 | 0.0213 | 0.0313 | 0.0133 | 0.0123 | 0.0047 | 0.0112 | 0.0098 | 0.0097 | 0.0065 |
Golgi | 0.0036 | 0.002 | 0.0063 | 0.0023 | 0.0126 | 0.0025 | 0.007 | 0.0011 | 0.0061 | 0.0041 | 0.0102 | 0.0047 | 0.0089 | 0.0007 | 0.0062 | 0.0014 | 0.0078 | 0.004 |
Lipid Particles | 0.0063 | 0.0102 | 0.0094 | 0.033 | 0.0128 | 0.0039 | 0.0112 | 0.0102 | 0.0163 | 0.0166 | 0.0271 | 0.0067 | 0.0108 | 0.0135 | 0.0101 | 0.0058 | 0.0058 | 0.004 |
Mitochondria | 0.0043 | 0.0006 | 0.0053 | 0.0009 | 0.0046 | 0.0004 | 0.0051 | 0.0004 | 0.0028 | 0.0053 | 0.0079 | 0.001 | 0.002 | 0.0001 | 0.0021 | 0.0001 | 0.003 | 0.019 |
None | 0.0257 | 0.0227 | 0.063 | 0.1185 | 0.072 | 0.0184 | 0.0467 | 0.0261 | 0.0674 | 0.2013 | 0.1236 | 0.0458 | 0.0471 | 0.0446 | 0.1315 | 0.1354 | 0.2472 | 0.1142 |
Nuclear Periphery | 0.0164 | 0.0062 | 0.0109 | 0.0051 | 0.0116 | 0.0069 | 0.0109 | 0.0101 | 0.0073 | 0.0105 | 0.0129 | 0.0054 | 0.0074 | 0.0071 | 0.0036 | 0.0055 | 0.0124 | 0.0031 |
Nucleolus | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0016 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0004 | 0.0009 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0043 | 0.0011 | 0.0026 | 0.0017 | 0.0026 | 0.0018 | 0.0014 | 0.0015 | 0.0022 | 0.0042 | 0.0046 | 0.0021 | 0.0008 | 0.0009 | 0.0011 | 0.001 | 0.0025 | 0.0009 |
Peroxisomes | 0.0044 | 0.0001 | 0.0014 | 0.0002 | 0.0051 | 0.0009 | 0.0062 | 0.0001 | 0.0065 | 0.0111 | 0.0021 | 0.001 | 0.0034 | 0 | 0.0013 | 0.0001 | 0.0039 | 0.0027 |
Punctate Nuclear | 0.0042 | 0.0008 | 0.0053 | 0.0007 | 0.0106 | 0.0018 | 0.0034 | 0.0004 | 0.0027 | 0.0044 | 0.0139 | 0.0063 | 0.0005 | 0.0003 | 0.0008 | 0.0005 | 0.0008 | 0.0004 |
Vacuole | 0.0049 | 0.0041 | 0.0064 | 0.004 | 0.0027 | 0.0039 | 0.0058 | 0.0056 | 0.0072 | 0.0121 | 0.0073 | 0.0042 | 0.002 | 0.0114 | 0.003 | 0.0036 | 0.0084 | 0.0033 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0008 | 0.0004 | 0.0013 | 0.0006 | 0.0011 | 0.0006 | 0.0006 | 0.0014 | 0.0029 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.7856 | 11.8217 | 47.1508 | 192.7303 | 45.5438 | 10.7388 | 7.4706 | 9.5801 | 138.441 | 101.8135 |
Translational Efficiency | 2.0384 | 0.6923 | 1.6224 | 1.2388 | 1.7628 | 0.7248 | 1.2332 | 1.7329 | 1.8839 | 1.6133 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1678 | 1857 | 361 | 1196 | 1446 | 1725 | 1294 | 135 | 3124 | 3582 | 1655 | 1331 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 759.63 | 891.51 | 1291.62 | 1158.59 | 818.35 | 835.82 | 1121.25 | 1411.24 | 786.81 | 864.69 | 1158.41 | 1184.22 |
Standard Deviation | 112.75 | 152.87 | 246.09 | 331.65 | 140.75 | 124.18 | 234.13 | 338.38 | 129.83 | 142.53 | 247.02 | 340.98 |
Intensity Change Log 2 | — | 0.230954 | 0.765813 | 0.609001 | — | 0.030474 | 0.454318 | 0.786173 | — | 0.130465 | 0.612671 | 0.703594 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000341 | 0.002854 | 0.001260 | 0.008151 | 0.001002 | 0.000961 | 0.004563 | 0.018446 | 0.000647 | 0.001943 | 0.003843 | 0.009195 |
Bud Neck | 0.008865 | 0.006945 | 0.002802 | 0.016947 | 0.007516 | 0.008720 | 0.010239 | 0.010797 | 0.008240 | 0.007800 | 0.008617 | 0.016323 |
Bud Site | 0.007669 | 0.023555 | 0.015447 | 0.061117 | 0.014675 | 0.014924 | 0.045357 | 0.113721* | 0.010912 | 0.019398 | 0.038833 | 0.066452 |
Cell Periphery | 0.001132 | 0.004100 | 0.007299 | 0.014951 | 0.002545 | 0.002239 | 0.012388 | 0.014296 | 0.001786 | 0.003204 | 0.011278 | 0.014885 |
Cytoplasm | 0.392978* | 0.265783* | 0.024904 | 0.266084* | 0.389366* | 0.280726* | 0.198010* | 0.144462* | 0.391306* | 0.272979* | 0.160251* | 0.253748* |
Cytoplasmic Foci | 0.135018 | 0.070841 | 0.004119 | 0.051526 | 0.104511 | 0.108884 | 0.019087 | 0.054685 | 0.120898 | 0.089162 | 0.015822 | 0.051846 |
Eisosomes | 0.000641 | 0.001213 | 0.000153 | 0.000824 | 0.000825 | 0.000825 | 0.000946 | 0.000542 | 0.000726 | 0.001026 | 0.000773 | 0.000795 |
Endoplasmic Reticulum | 0.236810* | 0.475801* | 0.904503* | 0.288380* | 0.294029* | 0.348687* | 0.590200* | 0.344775* | 0.263295* | 0.414586* | 0.658758* | 0.294100* |
Endosome | 0.012741 | 0.013074 | 0.006840 | 0.016286 | 0.010660 | 0.025915 | 0.010727 | 0.028755 | 0.011778 | 0.019258 | 0.009879 | 0.017551 |
Golgi | 0.009948 | 0.015070 | 0.001315 | 0.004227 | 0.010399 | 0.022053 | 0.005096 | 0.012282 | 0.010157 | 0.018433 | 0.004271 | 0.005044 |
Lipid Particles | 0.038551 | 0.028310 | 0.006755 | 0.052496 | 0.036247 | 0.057928 | 0.020668 | 0.059081 | 0.037485 | 0.042573 | 0.017633 | 0.053164 |
Mitochondria | 0.006291 | 0.008861 | 0.000352 | 0.002369 | 0.007342 | 0.013128 | 0.004403 | 0.000396 | 0.006777 | 0.010916 | 0.003519 | 0.002169 |
Mitotic Spindle | 0.001478 | 0.001077 | 0.000248 | 0.006830 | 0.001251 | 0.002882 | 0.000848 | 0.004301 | 0.001373 | 0.001946 | 0.000717 | 0.006574 |
None | 0.007345 | 0.007034 | 0.000191 | 0.009252 | 0.006319 | 0.005375 | 0.006415 | 0.000113 | 0.006870 | 0.006235 | 0.005057 | 0.008325 |
Nuclear Periphery | 0.003276 | 0.001092 | 0.000595 | 0.002410 | 0.002781 | 0.002903 | 0.000628 | 0.000679 | 0.003047 | 0.001964 | 0.000621 | 0.002234 |
Nuclear Periphery Foci | 0.004290 | 0.004235 | 0.006695 | 0.021219 | 0.004069 | 0.005124 | 0.007114 | 0.027502 | 0.004188 | 0.004663 | 0.007023 | 0.021856 |
Nucleolus | 0.000891 | 0.000377 | 0.000026 | 0.000847 | 0.000789 | 0.001110 | 0.000298 | 0.000061 | 0.000844 | 0.000730 | 0.000239 | 0.000767 |
Nucleus | 0.074843 | 0.029335 | 0.002472 | 0.056812 | 0.059712 | 0.032391 | 0.022991 | 0.006627 | 0.067839 | 0.030807 | 0.018515 | 0.051722 |
Peroxisomes | 0.003665 | 0.005122 | 0.000248 | 0.002352 | 0.002958 | 0.005368 | 0.002007 | 0.001610 | 0.003337 | 0.005241 | 0.001624 | 0.002277 |
Vacuole | 0.051340 | 0.032647 | 0.012970 | 0.115755 | 0.041295 | 0.054110 | 0.036531 | 0.155017 | 0.046691 | 0.042983 | 0.031392 | 0.119737 |
Vacuole Periphery | 0.001887 | 0.002672 | 0.000806 | 0.001165 | 0.001710 | 0.005747 | 0.001482 | 0.001853 | 0.001805 | 0.004153 | 0.001335 | 0.001235 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.53 | -3.10 | -7.97 | -5.05 | -6.47 | 0.19 | -6.06 | -2.96 | -2.97 | -2.29 | -6.60 | -7.09 | -8.04 | -6.70 | -4.48 |
Bud Neck | 1.73 | 5.08 | -4.73 | -6.09 | -8.43 | -1.11 | -2.24 | -1.88 | -1.24 | -0.36 | 0.57 | -0.49 | -5.59 | -5.98 | -4.86 |
Bud Site | -7.55 | -2.09 | -13.26 | -8.51 | -8.03 | -0.12 | -8.25 | -5.59 | -5.58 | -3.78 | -5.49 | -9.73 | -13.56 | -11.24 | -5.48 |
Cell Periphery | -7.01 | -3.95 | -9.34 | -6.86 | -3.09 | 0.50 | -8.04 | -2.80 | -2.89 | -0.23 | -3.91 | -9.80 | -9.23 | -8.09 | -1.52 |
Cytoplasm | 14.17 | 45.86 | 11.43 | -0.82 | -27.77 | 11.01 | 18.88 | 11.35 | 6.02 | 1.86 | 17.81 | 30.63 | 14.35 | 1.20 | -10.94 |
Cytoplasmic Foci | 12.85 | 31.18 | 16.79 | 4.83 | -15.10 | -0.83 | 20.94 | 5.40 | 5.90 | -4.02 | 8.79 | 33.89 | 17.73 | 10.55 | -11.53 |
Eisosomes | -3.94 | 6.79 | -1.34 | 2.89 | -6.84 | 0.00 | -0.88 | 1.91 | 1.94 | 2.49 | -3.20 | -0.47 | -0.16 | 2.70 | 0.29 |
Endoplasmic Reticulum | -19.49 | -47.60* | -1.77 | 15.91 | 42.47* | -4.06 | -20.13 | -0.62 | 0.97 | 7.81 | -16.59 | -34.93 | -0.21 | 12.72 | 29.04 |
Endosome | -0.34 | 4.85 | -2.27 | -2.06 | -6.02 | -10.82 | -0.17 | -4.92 | -0.65 | -4.87 | -8.74 | 2.35 | -4.47 | 1.78 | -5.82 |
Golgi | -4.73 | 11.48 | 7.40 | 11.37 | -4.22 | -6.97 | 4.93 | -0.21 | 2.35 | -1.42 | -8.44 | 8.31 | 6.66 | 13.59 | -0.39 |
Lipid Particles | 4.51 | 14.15 | -2.87 | -5.65 | -11.38 | -6.85 | 5.50 | -2.16 | -0.05 | -3.67 | -2.70 | 9.83 | -3.68 | -2.21 | -9.05 |
Mitochondria | -2.85 | 8.07 | 4.38 | 8.51 | -3.33 | -3.97 | 2.96 | 7.55 | 11.15 | 9.29 | -4.92 | 4.94 | 6.16 | 11.14 | 2.42 |
Mitotic Spindle | 0.52 | 1.93 | -4.34 | -4.77 | -5.63 | -1.90 | 0.64 | -1.51 | -0.93 | -1.63 | -1.03 | 1.33 | -4.84 | -4.31 | -5.44 |
None | 0.27 | 9.28 | -0.48 | -0.62 | -4.17 | 0.78 | 0.02 | 6.69 | 6.10 | 5.49 | 0.77 | 1.76 | -0.33 | -0.68 | -1.27 |
Nuclear Periphery | 4.59 | 5.80 | 1.25 | -3.42 | -4.77 | -0.22 | 4.32 | 4.15 | 6.67 | -0.33 | 2.90 | 7.13 | 1.46 | -0.98 | -4.80 |
Nuclear Periphery Foci | 0.10 | -2.17 | -9.99 | -9.97 | -7.18 | -1.64 | -3.61 | -5.19 | -4.97 | -4.44 | -1.18 | -4.30 | -11.14 | -10.85 | -8.70 |
Nucleolus | 3.27 | 5.31 | 0.75 | -2.71 | -5.13 | -0.63 | 2.20 | 3.16 | 3.12 | 7.22 | 0.82 | 4.46 | 0.97 | 0.06 | -3.52 |
Nucleus | 16.87 | 29.36 | 3.66 | -9.04 | -17.76 | 11.07 | 13.72 | 21.38 | 13.20 | 7.97 | 20.03 | 23.80 | 3.88 | -7.96 | -11.30 |
Peroxisomes | -2.97 | 11.23 | 3.89 | 6.48 | -8.33 | -5.51 | 2.86 | 3.59 | 7.95 | 1.20 | -5.76 | 6.43 | 4.21 | 9.20 | -1.95 |
Vacuole | 6.93 | 12.38 | -14.53 | -18.31 | -20.99 | -4.04 | 1.11 | -6.76 | -6.04 | -6.93 | 1.78 | 5.94 | -17.06 | -17.76 | -19.23 |
Vacuole Periphery | -3.74 | 3.50 | 4.62 | 7.65 | -0.93 | -7.78 | 0.87 | -0.10 | 5.78 | -0.52 | -8.59 | 2.46 | 4.12 | 10.76 | 0.95 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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