ORF | |
---|---|
Human Ortholog | |
Description | Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 4 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 4 | 1 | 3 | 3 | 4 | 5 | 9 |
Bud | 1 | 2 | 0 | 12 | 5 | 7 | 12 | 14 | 10 | 20 | 5 | 3 | 3 | 1 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 7 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 14 | 12 | 5 | 4 | 0 | 2 | 3 | 0 | 4 | 0 | 3 | 4 | 6 | 7 | 8 | 5 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 3 | 0 | 6 | 6 | 3 | 9 | 8 | 3 | 3 | 2 | 4 | 5 | 8 | 7 | 14 | 10 | 3 | 0 | 1 | 0 | 3 | 0 | 0 |
Endoplasmic Reticulum | 2 | 3 | 3 | 4 | 0 | 3 | 4 | 2 | 1 | 1 | 1 | 2 | 0 | 2 | 2 | 3 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 1 | 3 | 5 | 5 | 20 | 7 | 9 | 18 | 0 | 1 | 1 | 3 | 3 | 3 | 4 | 6 | 5 | 1 | 5 | 7 | 10 | 3 | 12 | 8 |
Golgi | 0 | 13 | 24 | 6 | 5 | 0 | 2 | 5 | 2 | 0 | 0 | 1 | 7 | 12 | 29 | 43 | 64 | 19 | 2 | 20 | 15 | 11 | 18 | 14 |
Mitochondria | 126 | 220 | 285 | 232 | 257 | 382 | 475 | 427 | 316 | 362 | 177 | 167 | 138 | 165 | 192 | 6 | 10 | 4 | 110 | 194 | 242 | 107 | 129 | 143 |
Nucleus | 2 | 1 | 1 | 1 | 0 | 4 | 5 | 5 | 2 | 1 | 1 | 2 | 1 | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 10 | 14 | 16 | 9 | 16 | 45 | 61 | 58 | 25 | 37 | 23 | 14 | 7 | 5 | 5 | 6 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | 1 |
Nucleolus | 12 | 4 | 5 | 6 | 10 | 32 | 44 | 42 | 25 | 29 | 23 | 13 | 2 | 1 | 4 | 4 | 4 | 1 | 0 | 0 | 0 | 1 | 2 | 2 |
Peroxisomes | 0 | 6 | 7 | 6 | 13 | 6 | 13 | 7 | 0 | 2 | 2 | 3 | 2 | 4 | 10 | 5 | 7 | 0 | 4 | 11 | 24 | 8 | 17 | 15 |
SpindlePole | 1 | 1 | 0 | 1 | 5 | 3 | 16 | 12 | 0 | 4 | 0 | 2 | 0 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 15 | 32 | 20 | 2 | 25 | 26 | 33 | 21 | 9 | 11 | 10 | 7 | 13 | 18 | 20 | 15 | 26 | 9 | 2 | 9 | 2 | 1 | 3 | 9 |
Unique Cell Count | 140 | 248 | 302 | 252 | 298 | 419 | 540 | 494 | 328 | 384 | 187 | 187 | 150 | 189 | 227 | 97 | 109 | 37 | 133 | 258 | 304 | 146 | 199 | 220 |
Labelled Cell Count | 177 | 317 | 382 | 296 | 366 | 520 | 691 | 629 | 394 | 478 | 246 | 228 | 185 | 227 | 283 | 118 | 144 | 46 | 133 | 258 | 304 | 146 | 199 | 220 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 8.0 | 7.4 | 6.7 | 7.0 | 6.3 | 5.7 | 6.2 | 6.1 | 5.4 | 5.6 | 5.8 | 7.4 | 7.7 | 7.9 | 11.0 | 12.7 | 13.9 | 7.9 | 7.8 | 8.7 |
Std Deviation (1e-4) | 1.4 | 1.4 | 1.3 | 1.3 | 1.4 | 1.1 | 1.3 | 1.3 | 1.2 | 1.3 | 1.1 | 1.7 | 1.5 | 1.4 | 1.4 | 3.1 | 2.9 | 3.1 | 2.4 | 2.0 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.08 | -0.24 | -0.37 | -0.24 | -0.28 | -0.44 | -0.39 | -0.35 | 0.0 | 0.06 | 0.1 | 0.57 | 0.78 | 0.91 | 0.1 | 0.08 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 3.8 | 0 | 0 | 0 | 3.0 | 0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | -2.0 | -4.1 | -3.9 | -3.4 | -3.6 | -2.5 | 0 | -1.5 | -0.7 | -0.5 | -0.5 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.1 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.9 | -3.6 | -5.9 | -6.1 | -5.1 | -4.6 | -5.6 | -4.0 | -3.6 | -1.3 | -0.7 | 1.8 | 8.3 | 11.1 | 0 |
Mitochondria | -1.1 | -3.4 | -1.6 | -3.0 | -3.5 | 1.2 | -0.1 | 0.1 | -2.1 | -1.0 | -2.8 | -3.7 | -17.0 | -16.9 | -13.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.0 | 0 | 2.6 | 2.9 | 3.0 | 1.2 | 2.1 | 2.8 | 1.0 | -0.3 | -1.4 | -1.8 | 0.3 | 0 | 0 |
Nucleolus | 0.6 | 1.3 | 3.6 | 3.9 | 4.0 | 3.5 | 3.5 | 4.9 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1.4 | -0.9 | 0.1 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.5 | 0.8 | -0.2 | -0.3 | -1.5 | -2.3 | -2.4 | -0.6 | -1.4 | 0.8 | 1.2 | 0.9 | 2.7 | 4.9 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0542 | 0.0276 | 0.0221 | 0.0008 | 0.0011 | 0.007 | 0.0562 | 0.0095 | 0.0237 | 0.0016 | 0.0046 | 0.0032 | 0.1132 | 0.0766 | 0.0464 | 0.0007 | 0.0034 | 0.0254 |
Bud | 0.0004 | 0.0016 | 0.0061 | 0.0002 | 0.0002 | 0.0007 | 0.001 | 0.0005 | 0.0011 | 0.0002 | 0.0043 | 0.0002 | 0.0003 | 0.0005 | 0.0009 | 0.0001 | 0.0003 | 0.0001 |
Bud Neck | 0.0004 | 0.0004 | 0.0033 | 0.0001 | 0.0002 | 0.0009 | 0.0011 | 0.0003 | 0.0014 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0006 | 0.0009 | 0.0006 | 0.0001 | 0.0005 |
Bud Periphery | 0.0009 | 0.0018 | 0.0229 | 0.0005 | 0.0005 | 0.003 | 0.0018 | 0.0011 | 0.0014 | 0.0011 | 0.0273 | 0.0004 | 0.0004 | 0.0007 | 0.0031 | 0.0001 | 0.0012 | 0.0003 |
Bud Site | 0.0013 | 0.0195 | 0.0117 | 0.0002 | 0.0004 | 0.0004 | 0.0056 | 0.003 | 0.0344 | 0.0002 | 0.0009 | 0.0002 | 0.0008 | 0.0017 | 0.0033 | 0.0001 | 0.0002 | 0.0002 |
Cell Periphery | 0.0003 | 0.0003 | 0.0122 | 0.0001 | 0.0002 | 0.0005 | 0.0008 | 0.0004 | 0.0027 | 0.0003 | 0.0019 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0.0021 | 0.001 | 0.0013 | 0 | 0.0001 | 0.0003 | 0.0057 | 0.0003 | 0.0045 | 0.0011 | 0.0005 | 0.0018 | 0 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0054 | 0.0138 | 0.0034 | 0.001 | 0.0034 | 0.0031 | 0.0604 | 0.0239 | 0.0281 | 0.0027 | 0.0146 | 0.0163 | 0.0017 | 0.0093 | 0.0061 | 0.0272 | 0.0007 | 0.0038 |
Eisosomes | 0.0012 | 0.0005 | 0.0014 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0005 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0017 | 0.0005 | 0.0015 | 0.0001 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0035 | 0.0007 | 0.0006 | 0.0001 | 0.0002 | 0.0008 | 0.0457 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Endosome | 0.0168 | 0.024 | 0.0049 | 0.0089 | 0.0231 | 0.0202 | 0.1005 | 0.0088 | 0.0263 | 0.0154 | 0.0084 | 0.018 | 0.0036 | 0.0086 | 0.0082 | 0.0066 | 0.0119 | 0.0057 |
Golgi | 0.0167 | 0.0207 | 0.0101 | 0.008 | 0.022 | 0.0172 | 0.0596 | 0.0147 | 0.0191 | 0.0037 | 0.0161 | 0.032 | 0.027 | 0.0393 | 0.0308 | 0.0153 | 0.0168 | 0.0184 |
Lipid Particles | 0.0169 | 0.0384 | 0.011 | 0.0013 | 0.0302 | 0.0096 | 0.0567 | 0.0533 | 0.0136 | 0.0017 | 0.0625 | 0.0311 | 0.0033 | 0.0015 | 0.001 | 0.0508 | 0.0022 | 0.0086 |
Mitochondria | 0.8109 | 0.798 | 0.8642 | 0.9724 | 0.8947 | 0.9047 | 0.5136 | 0.8559 | 0.7794 | 0.959 | 0.7754 | 0.8441 | 0.8396 | 0.8331 | 0.8782 | 0.798 | 0.9544 | 0.9203 |
None | 0.0068 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0045 | 0.0001 | 0.0007 | 0.0018 | 0.0004 | 0.0112 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0184 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0017 | 0.0304 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Nucleolus | 0.0008 | 0.0008 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0045 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0028 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.025 | 0.0453 | 0.0164 | 0.0025 | 0.0154 | 0.022 | 0.0349 | 0.0248 | 0.0529 | 0.0056 | 0.0777 | 0.0369 | 0.0065 | 0.0258 | 0.0143 | 0.0968 | 0.0011 | 0.013 |
Punctate Nuclear | 0.0062 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0095 | 0.0006 | 0.0002 | 0 | 0.0007 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 |
Vacuole | 0.0029 | 0.0026 | 0.0032 | 0.0012 | 0.0032 | 0.0026 | 0.0039 | 0.0008 | 0.0037 | 0.0023 | 0.0017 | 0.0008 | 0.0002 | 0.0002 | 0.0007 | 0.0011 | 0.0018 | 0.0008 |
Vacuole Periphery | 0.0043 | 0.0024 | 0.0037 | 0.0022 | 0.0042 | 0.0044 | 0.004 | 0.0007 | 0.0048 | 0.0018 | 0.0016 | 0.0013 | 0.0006 | 0.0004 | 0.0015 | 0.0018 | 0.0044 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.9412 | 18.2662 | 18.5242 | 19.2755 | 12.8049 | 21.1028 | 27.0878 | 28.878 | 24.5089 | 23.8271 |
Translational Efficiency | 0.5351 | 0.8275 | 0.5444 | 0.6254 | 0.8241 | 0.5074 | 0.6944 | 0.5124 | 0.6439 | 0.6135 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1281 | 1484 | 587 | 1818 | 1530 | 1939 | 1548 | 59 | 2811 | 3423 | 2135 | 1877 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1058.98 | 1483.90 | 1832.16 | 1458.58 | 1346.81 | 1494.82 | 1546.07 | 1609.09 | 1215.64 | 1490.09 | 1624.73 | 1463.31 |
Standard Deviation | 156.42 | 289.65 | 233.94 | 233.38 | 217.96 | 259.08 | 260.33 | 292.62 | 239.91 | 272.81 | 283.73 | 236.93 |
Intensity Change Log 2 | — | 0.486719 | 0.790870 | 0.461889 | — | 0.150425 | 0.199059 | 0.256699 | — | 0.308182 | 0.489757 | 0.350633 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000139 | 0.000379 | 0.000400 | 0.001565 | 0.000202 | 0.000118 | 0.000369 | 0.001391 | 0.000174 | 0.000231 | 0.000377 | 0.001559 |
Bud Neck | 0.000379 | 0.000482 | 0.002081 | 0.003423 | 0.000531 | 0.000412 | 0.001651 | 0.003351 | 0.000462 | 0.000443 | 0.001769 | 0.003421 |
Bud Site | 0.001922 | 0.002374 | 0.001775 | 0.010614 | 0.003053 | 0.001171 | 0.003979 | 0.008926 | 0.002538 | 0.001693 | 0.003373 | 0.010561 |
Cell Periphery | 0.000075 | 0.000074 | 0.000201 | 0.000102 | 0.000060 | 0.000031 | 0.000154 | 0.000216 | 0.000067 | 0.000050 | 0.000167 | 0.000105 |
Cytoplasm | 0.818962* | 0.713393* | 0.430066* | 0.541956* | 0.762880* | 0.723950* | 0.502569* | 0.511334* | 0.788437* | 0.719373* | 0.482635* | 0.540994* |
Cytoplasmic Foci | 0.019452 | 0.006316 | 0.004611 | 0.005158 | 0.008847 | 0.004736 | 0.003441 | 0.027911 | 0.013680 | 0.005421 | 0.003763 | 0.005873 |
Eisosomes | 0.000007 | 0.000003 | 0.000004 | 0.000007 | 0.000004 | 0.000001 | 0.000013 | 0.000025 | 0.000005 | 0.000002 | 0.000010 | 0.000008 |
Endoplasmic Reticulum | 0.000795 | 0.000353 | 0.000534 | 0.000512 | 0.000666 | 0.000141 | 0.001162 | 0.000782 | 0.000725 | 0.000233 | 0.000990 | 0.000520 |
Endosome | 0.000774 | 0.000594 | 0.000467 | 0.005099 | 0.000972 | 0.000291 | 0.001117 | 0.074714 | 0.000882 | 0.000422 | 0.000939 | 0.007287 |
Golgi | 0.000632 | 0.001127 | 0.000254 | 0.005424 | 0.000521 | 0.000734 | 0.000541 | 0.002320 | 0.000572 | 0.000905 | 0.000462 | 0.005326 |
Lipid Particles | 0.000494 | 0.000268 | 0.000779 | 0.000709 | 0.000251 | 0.000093 | 0.000604 | 0.005279 | 0.000362 | 0.000169 | 0.000652 | 0.000853 |
Mitochondria | 0.000385 | 0.000957 | 0.000500 | 0.003364 | 0.000180 | 0.000176 | 0.000982 | 0.000730 | 0.000273 | 0.000515 | 0.000849 | 0.003282 |
Mitotic Spindle | 0.001777 | 0.001869 | 0.005610 | 0.061276 | 0.002100 | 0.005201 | 0.006458 | 0.069789 | 0.001953 | 0.003757 | 0.006225 | 0.061543 |
None | 0.007718 | 0.015394 | 0.012081 | 0.019154 | 0.007748 | 0.015490 | 0.012641 | 0.007208 | 0.007734 | 0.015448 | 0.012487 | 0.018778 |
Nuclear Periphery | 0.000125 | 0.000113 | 0.000829 | 0.001293 | 0.000166 | 0.000095 | 0.000747 | 0.000433 | 0.000147 | 0.000103 | 0.000770 | 0.001266 |
Nuclear Periphery Foci | 0.002501 | 0.002475 | 0.001773 | 0.002448 | 0.003845 | 0.001024 | 0.002195 | 0.004315 | 0.003232 | 0.001653 | 0.002079 | 0.002506 |
Nucleolus | 0.000214 | 0.000135 | 0.000171 | 0.000287 | 0.000103 | 0.000208 | 0.000175 | 0.000165 | 0.000154 | 0.000176 | 0.000174 | 0.000283 |
Nucleus | 0.131363* | 0.245039* | 0.516863* | 0.300054* | 0.196367* | 0.241047* | 0.415400* | 0.155525* | 0.166744* | 0.242777* | 0.443296* | 0.295511* |
Peroxisomes | 0.000199 | 0.000250 | 0.000042 | 0.001042 | 0.000213 | 0.000137 | 0.000236 | 0.006098 | 0.000206 | 0.000186 | 0.000182 | 0.001201 |
Vacuole | 0.011780 | 0.007826 | 0.020873 | 0.033036 | 0.011170 | 0.004826 | 0.045020 | 0.119211 | 0.011448 | 0.006126 | 0.038381 | 0.035745 |
Vacuole Periphery | 0.000304 | 0.000578 | 0.000086 | 0.003477 | 0.000123 | 0.000117 | 0.000547 | 0.000278 | 0.000206 | 0.000317 | 0.000420 | 0.003376 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.02 | -10.30 | -2.87 | -0.36 | 2.51 | 2.41 | -3.12 | -3.25 | -3.95 | -2.02 | -2.31 | -8.35 | -2.63 | -1.85 | 0.02 |
Bud Neck | -5.50 | -15.45 | -15.30 | -13.68 | 3.27 | 0.87 | -7.83 | -5.53 | -5.73 | -2.08 | -1.44 | -14.15 | -14.97 | -14.73 | -2.34 |
Bud Site | -1.41 | -4.57 | -5.01 | -3.37 | -0.55 | 2.25 | -0.16 | -3.95 | -6.90 | -4.32 | 1.41 | -1.76 | -3.71 | -5.52 | -2.26 |
Cell Periphery | -2.26 | -13.10 | -6.14 | -2.92 | 11.44 | 6.76 | -5.41 | -4.53 | -5.37 | -2.18 | 1.69 | -11.51 | -9.33 | -10.61 | 7.01 |
Cytoplasm | 17.15 | 49.13 | 52.72 | 34.57 | -9.14 | 6.79 | 47.09 | 11.05 | 9.93 | 1.56 | 16.59 | 66.92 | 54.72 | 42.85 | -6.01 |
Cytoplasmic Foci | 9.34 | 8.77 | 13.58 | 4.09 | 6.30 | 4.83 | 9.08 | -0.31 | -2.15 | -3.37 | 10.31 | 14.33 | 14.03 | 3.66 | -0.71 |
Eisosomes | 3.17 | -4.93 | -7.88 | -11.94 | -1.91 | 5.22 | -7.56 | -7.66 | -8.49 | -4.60 | 5.61 | -8.00 | -13.90 | -20.25 | -1.38 |
Endoplasmic Reticulum | 4.88 | -1.61 | 2.29 | -4.31 | 4.15 | 6.13 | -2.90 | -1.67 | -5.42 | 0.07 | 7.95 | -3.31 | 1.60 | -10.49 | 6.17 |
Endosome | 1.30 | -1.65 | -1.08 | -1.99 | -0.01 | 1.65 | 0.38 | -1.05 | -1.14 | -1.07 | 2.05 | 0.07 | -1.12 | -2.87 | -1.39 |
Golgi | -0.74 | 0.38 | -0.26 | 0.44 | -0.67 | -0.42 | 1.10 | 0.03 | 0.43 | -0.92 | -0.82 | 1.14 | -0.48 | 0.07 | -1.10 |
Lipid Particles | 0.54 | -6.90 | -3.99 | -11.70 | 5.45 | 5.36 | -8.05 | -4.85 | -5.21 | -3.75 | 1.53 | -7.44 | -9.03 | -18.10 | -1.54 |
Mitochondria | -0.91 | -1.89 | -1.76 | -0.38 | -0.95 | 0.03 | -2.79 | -1.66 | -1.56 | 1.98 | -0.85 | -2.79 | -2.30 | -1.52 | -0.82 |
Mitotic Spindle | -0.07 | -4.38 | -8.75 | -9.06 | -5.06 | -1.99 | -5.02 | -2.39 | -1.99 | -1.38 | -1.79 | -6.46 | -9.20 | -8.33 | -5.65 |
None | -6.71 | -1.75 | -6.47 | 0.52 | -4.55 | -11.31 | -5.93 | 0.53 | 3.90 | 2.48 | -12.44 | -5.99 | -7.44 | 1.35 | -2.70 |
Nuclear Periphery | -1.55 | -19.69 | -22.90 | -22.82 | 0.47 | 5.76 | -22.84 | -6.88 | -7.23 | -1.31 | 3.05 | -30.03 | -23.17 | -23.66 | -4.34 |
Nuclear Periphery Foci | -1.07 | -2.22 | 4.46 | 5.61 | 6.26 | 9.51 | 6.50 | 0.67 | -3.12 | -2.12 | 6.56 | 4.49 | 8.75 | 2.71 | 4.59 |
Nucleolus | 0.85 | -0.80 | -1.37 | -7.11 | -2.49 | -1.33 | -4.31 | -2.70 | 0.47 | 1.72 | -0.63 | -2.31 | -3.91 | -2.11 | -3.39 |
Nucleus | -19.74 | -38.43 | -33.15 | -12.94 | 16.07 | -8.65 | -36.35 | 0.62 | 2.66 | 11.43 | -19.52 | -52.92 | -29.39 | -15.11 | 19.28 |
Peroxisomes | -0.10 | 1.26 | 0.23 | 0.32 | -0.81 | 0.46 | 0.76 | -0.88 | -0.98 | -1.04 | 0.31 | 1.25 | 0.04 | -0.20 | -0.91 |
Vacuole | 0.27 | -12.27 | -27.89 | -28.16 | -8.89 | 4.63 | -16.06 | -9.15 | -9.33 | -7.42 | 3.82 | -20.19 | -28.83 | -29.87 | -12.37 |
Vacuole Periphery | -0.68 | 1.02 | -0.88 | -0.09 | -1.76 | 0.25 | -1.03 | -0.71 | -0.89 | 0.77 | -0.55 | -0.07 | -1.48 | -1.06 | -1.43 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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