Standard name | |
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Human Ortholog | |
Description | Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 1 | 1 | 4 | – | 4 | 6 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 8 | 1 | – | 8 | 10 | 5 | 10 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 1 | 3 | 12 | 20 | 16 | 24 | – | 14 | 12 | 14 | 4 | 18 | 59 | 114 | 22 | 20 | 20 | 0 | 0 | 0 | 0 | 2 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 4 | 8 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 2 | 2 | 0 | 6 | 8 | 2 | 9 | – | 2 | 1 | 1 | 0 | 1 | 2 | 11 | 9 | 8 | 12 | 7 | 1 | 1 | 2 | 11 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 0 |
Mitochondria | 27 | 23 | 4 | 21 | 16 | 156 | 31 | – | 106 | 100 | 79 | 48 | 13 | 11 | 2 | 33 | 19 | 23 | 1 | 1 | 1 | 2 | 3 | 7 |
Nucleus | 157 | 79 | 78 | 259 | 397 | 270 | 487 | – | 273 | 349 | 189 | 141 | 458 | 571 | 624 | 302 | 237 | 222 | 148 | 89 | 78 | 166 | 422 | 377 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 3 | 1 | 6 | – | 3 | 4 | 3 | 0 | 0 | 2 | 2 | 1 | 1 | 3 | 2 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 12 | 3 | 0 | 3 | 12 | 8 | 20 | – | 26 | 40 | 34 | 31 | 17 | 9 | 23 | 5 | 4 | 5 | 23 | 12 | 17 | 52 | 120 | 126 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 3 | 1 | 6 | 16 | 12 | 12 | 23 | – | 4 | 7 | 5 | 2 | 11 | 15 | 8 | 17 | 18 | 14 | 4 | 3 | 7 | 8 | 31 | 32 |
Vac/Vac Membrane | 3 | 6 | 0 | 2 | 9 | 2 | 6 | – | 3 | 1 | 0 | 1 | 1 | 0 | 6 | 11 | 12 | 5 | 10 | 10 | 12 | 16 | 45 | 44 |
Unique Cell Count | 179 | 100 | 85 | 290 | 447 | 347 | 559 | 348 | 428 | 263 | 172 | 495 | 647 | 771 | 352 | 282 | 278 | 205 | 121 | 125 | 256 | 651 | 618 | |
Labelled Cell Count | 209 | 115 | 91 | 321 | 478 | 477 | 612 | 444 | 533 | 331 | 237 | 520 | 673 | 795 | 409 | 326 | 318 | 205 | 121 | 125 | 256 | 651 | 618 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.8 | 5.0 | 5.4 | 6.1 | 4.4 | 5.3 | – | 4.9 | 5.0 | 5.3 | 4.9 | 5.5 | 5.2 | 5.9 | 6.2 | 6.6 | 6.4 | 4.8 | 5.2 | 5.3 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.0 | 1.3 | 1.6 | 1.5 | 1.2 | – | 1.5 | 1.3 | 1.7 | 1.1 | 0.9 | 1.1 | 1.4 | 1.1 | 1.1 | 1.4 | 1.1 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.3 | -0.19 | 0.1 | – | -0.01 | 0.01 | 0.1 | -0.04 | 0.15 | 0.07 | 0.23 | 0.31 | 0.4 | 0.35 | -0.05 | 0.05 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7005 | 0.5678 | 0.6904 | 0.1538 | 0.1304 | 0.6981 | 1.2033 | 1.5089 | 1.19 | 0.9254 | 1.255 | 1.2037 | 3.4576 | 3.576 | 3.2529 | 3.085 | 3.2848 | 3.2457 |
Actin | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0.0253 | 0 | 0.0118 | 0.0001 | 0.0014 | 0 |
Bud | 0.0001 | 0 | 0 | 0.0006 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0008 | 0.0019 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0064 | 0.0002 | 0.0026 | 0.0079 | 0.0014 | 0.001 |
Bud Periphery | 0.0002 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0026 | 0 | 0.0001 | 0.0004 | 0 | 0 |
Bud Site | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0.0021 | 0.0001 | 0.0018 | 0.0004 | 0.0001 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0027 | 0 | 0 | 0.0004 | 0 | 0 |
Cytoplasm | 0.0006 | 0.0008 | 0.0046 | 0.0261 | 0.0001 | 0.0029 | 0.0014 | 0.0017 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0022 | 0.0001 | 0.0002 | 0.0002 | 0.001 | 0.0001 |
Cytoplasmic Foci | 0.0008 | 0 | 0.0007 | 0.0079 | 0 | 0.0024 | 0.0022 | 0.0002 | 0 | 0 | 0 | 0 | 0.0081 | 0 | 0.0006 | 0 | 0.0045 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0003 | 0 | 0.0008 | 0.0002 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0027 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endosome | 0.003 | 0.0001 | 0.0015 | 0.0252 | 0 | 0.0013 | 0.0035 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0089 | 0 | 0.0009 | 0.0001 | 0.0028 | 0 |
Golgi | 0.0008 | 0 | 0.0001 | 0.0031 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0043 | 0 | 0.0009 | 0 | 0.0012 | 0 |
Lipid Particles | 0.0034 | 0 | 0.0002 | 0.0132 | 0 | 0.0002 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0044 | 0 | 0.0006 | 0 | 0.0126 | 0 |
Mitochondria | 0.0051 | 0.0002 | 0.0002 | 0.0027 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0017 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0001 |
None | 0.0002 | 0.0005 | 0.0017 | 0.0041 | 0.0001 | 0.0003 | 0.0013 | 0.0081 | 0 | 0.0001 | 0.0001 | 0 | 0.0018 | 0 | 0.0006 | 0 | 0.0011 | 0.0001 |
Nuclear Periphery | 0.0066 | 0.0045 | 0.0067 | 0.0013 | 0.0028 | 0.0016 | 0.0014 | 0.0016 | 0.0043 | 0.0014 | 0.0009 | 0.0001 | 0.0119 | 0.0003 | 0.0013 | 0.0021 | 0.0027 | 0.0007 |
Nucleolus | 0.0209 | 0.0353 | 0.035 | 0.0167 | 0.0931 | 0.0775 | 0.0321 | 0.0332 | 0.0229 | 0.0195 | 0.0833 | 0.0765 | 0.0341 | 0.0404 | 0.032 | 0.0277 | 0.0664 | 0.0605 |
Nucleus | 0.9397 | 0.9544 | 0.9322 | 0.8365 | 0.8993 | 0.8795 | 0.9462 | 0.9518 | 0.9703 | 0.9775 | 0.8905 | 0.9154 | 0.838 | 0.9557 | 0.9438 | 0.9568 | 0.879 | 0.9338 |
Peroxisomes | 0.0018 | 0 | 0 | 0.0012 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.007 | 0 | 0.0004 | 0 | 0.0142 | 0 |
Punctate Nuclear | 0.014 | 0.0036 | 0.015 | 0.0557 | 0.003 | 0.0301 | 0.0076 | 0.0023 | 0.0017 | 0.001 | 0.0244 | 0.0068 | 0.0317 | 0.0031 | 0.0015 | 0.0029 | 0.0109 | 0.0036 |
Vacuole | 0.0012 | 0.0001 | 0.0002 | 0.0029 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0022 | 0 | 0.0001 | 0.0006 | 0.0001 | 0 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.126 | 29.0111 | 22.9626 | 24.5884 | 19.6359 | 30.2728 | 18.0887 | 25.7372 | 22.7355 | 27.3758 |
Translational Efficiency | 0.3661 | 0.3402 | 0.3736 | 0.2052 | 0.5262 | 0.3172 | 0.3981 | 0.3013 | 0.4935 | 0.4466 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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