Standard name
Human Ortholog
Description Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 – 0 0 0.05 0 0 0.09 0.15 0.06 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.23 0.05 0.07 0 0.45 0.06 – 0.3 0.23 0.3 0.28 0 0 0 0.09 0.07 0.08 0 0 0 0 0 0
Nucleus 0.88 0.79 0.92 0.89 0.89 0.78 0.87 – 0.78 0.82 0.72 0.82 0.93 0.88 0.81 0.86 0.84 0.8 0.72 0.74 0.63 0.65 0.65 0.61
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 – 0.07 0.09 0.13 0.18 0 0 0 0 0 0 0.12 0.11 0.14 0.21 0.19 0.2
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.06 0 0 0 – 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0.06 0 0.05 0.05
Vac/Vac Membrane 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.1 0.07 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 1 1 4 – 4 6 1 0 0 1 0 3 0 1 1 0 0 0 4 4
Bud Neck 0 0 0 0 0 1 0 – 1 3 0 0 1 0 0 0 0 0 0 0 0 1 2 3
Bud Site 0 0 0 1 0 8 1 – 8 10 5 10 0 0 0 1 0 1 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 3 1 3 12 20 16 24 – 14 12 14 4 18 59 114 22 20 20 0 0 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 4 4 8 0 0 0 0 1 0
Endosome 2 2 0 6 8 2 9 – 2 1 1 0 1 2 11 9 8 12 7 1 1 2 11 6
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 1 0 1 1 1 0
Mitochondria 27 23 4 21 16 156 31 – 106 100 79 48 13 11 2 33 19 23 1 1 1 2 3 7
Nucleus 157 79 78 259 397 270 487 – 273 349 189 141 458 571 624 302 237 222 148 89 78 166 422 377
Nuclear Periphery 1 0 0 0 3 1 6 – 3 4 3 0 0 2 2 1 1 3 2 0 0 0 1 3
Nucleolus 12 3 0 3 12 8 20 – 26 40 34 31 17 9 23 5 4 5 23 12 17 52 120 126
Peroxisomes 0 0 0 0 0 0 1 – 0 0 0 0 0 2 3 0 1 3 0 0 0 0 0 1
SpindlePole 3 1 6 16 12 12 23 – 4 7 5 2 11 15 8 17 18 14 4 3 7 8 31 32
Vac/Vac Membrane 3 6 0 2 9 2 6 – 3 1 0 1 1 0 6 11 12 5 10 10 12 16 45 44
Unique Cell Count 179 100 85 290 447 347 559 348 428 263 172 495 647 771 352 282 278 205 121 125 256 651 618
Labelled Cell Count 209 115 91 321 478 477 612 444 533 331 237 520 673 795 409 326 318 205 121 125 256 651 618


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.8 5.0 5.4 6.1 4.4 5.3 – 4.9 5.0 5.3 4.9 5.5 5.2 5.9 6.2 6.6 6.4 4.8 5.2 5.3
Std Deviation (1e-4) 0.7 0.7 1.0 1.3 1.6 1.5 1.2 – 1.5 1.3 1.7 1.1 0.9 1.1 1.4 1.1 1.1 1.4 1.1 1.1 1.3
Intensity Change (Log2) – – – 0.11 0.3 -0.19 0.1 – -0.01 0.01 0.1 -0.04 0.15 0.07 0.23 0.31 0.4 0.35 -0.05 0.05 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7005 0.5678 0.6904 0.1538 0.1304 0.6981 1.2033 1.5089 1.19 0.9254 1.255 1.2037 3.4576 3.576 3.2529 3.085 3.2848 3.2457
Actin 0.0003 0.0001 0.0005 0.0001 0.0001 0.0002 0.0002 0.0002 0 0 0 0 0.0253 0 0.0118 0.0001 0.0014 0
Bud 0.0001 0 0 0.0006 0 0.0004 0.0001 0 0 0 0 0 0.0005 0 0.0001 0.0001 0 0
Bud Neck 0.0003 0.0002 0.0003 0.0003 0.0008 0.0019 0.001 0.0001 0.0001 0.0001 0.0002 0.001 0.0064 0.0002 0.0026 0.0079 0.0014 0.001
Bud Periphery 0.0002 0 0 0.0002 0 0.0002 0.0001 0 0 0 0 0 0.0026 0 0.0001 0.0004 0 0
Bud Site 0.0002 0.0001 0.0002 0.0003 0.0001 0.0002 0.0007 0.0001 0 0 0 0 0.0021 0.0001 0.0018 0.0004 0.0001 0.0001
Cell Periphery 0 0 0 0.0001 0 0 0.0001 0 0 0 0 0 0.0027 0 0 0.0004 0 0
Cytoplasm 0.0006 0.0008 0.0046 0.0261 0.0001 0.0029 0.0014 0.0017 0.0002 0.0001 0.0002 0 0.0022 0.0001 0.0002 0.0002 0.001 0.0001
Cytoplasmic Foci 0.0008 0 0.0007 0.0079 0 0.0024 0.0022 0.0002 0 0 0 0 0.0081 0 0.0006 0 0.0045 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0007 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0003 0 0.0008 0.0002 0 0.0004 0.0002 0.0001 0.0001 0 0 0 0.0027 0 0.0002 0.0001 0.0001 0
Endosome 0.003 0.0001 0.0015 0.0252 0 0.0013 0.0035 0.0002 0.0001 0 0 0 0.0089 0 0.0009 0.0001 0.0028 0
Golgi 0.0008 0 0.0001 0.0031 0 0.0002 0.0002 0 0 0 0 0 0.0043 0 0.0009 0 0.0012 0
Lipid Particles 0.0034 0 0.0002 0.0132 0 0.0002 0.0005 0.0001 0 0 0 0 0.0044 0 0.0006 0 0.0126 0
Mitochondria 0.0051 0.0002 0.0002 0.0027 0.0004 0.0002 0.0005 0.0001 0.0001 0.0001 0.0001 0 0.0017 0.0001 0.0002 0.0003 0.0004 0.0001
None 0.0002 0.0005 0.0017 0.0041 0.0001 0.0003 0.0013 0.0081 0 0.0001 0.0001 0 0.0018 0 0.0006 0 0.0011 0.0001
Nuclear Periphery 0.0066 0.0045 0.0067 0.0013 0.0028 0.0016 0.0014 0.0016 0.0043 0.0014 0.0009 0.0001 0.0119 0.0003 0.0013 0.0021 0.0027 0.0007
Nucleolus 0.0209 0.0353 0.035 0.0167 0.0931 0.0775 0.0321 0.0332 0.0229 0.0195 0.0833 0.0765 0.0341 0.0404 0.032 0.0277 0.0664 0.0605
Nucleus 0.9397 0.9544 0.9322 0.8365 0.8993 0.8795 0.9462 0.9518 0.9703 0.9775 0.8905 0.9154 0.838 0.9557 0.9438 0.9568 0.879 0.9338
Peroxisomes 0.0018 0 0 0.0012 0 0 0.0003 0 0 0 0 0 0.007 0 0.0004 0 0.0142 0
Punctate Nuclear 0.014 0.0036 0.015 0.0557 0.003 0.0301 0.0076 0.0023 0.0017 0.001 0.0244 0.0068 0.0317 0.0031 0.0015 0.0029 0.0109 0.0036
Vacuole 0.0012 0.0001 0.0002 0.0029 0.0001 0.0003 0.0004 0.0001 0.0001 0 0 0 0.0022 0 0.0001 0.0006 0.0001 0
Vacuole Periphery 0.0007 0.0001 0.0001 0.0015 0.0001 0.0001 0.0002 0 0 0 0 0 0.0007 0 0.0001 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.126 29.0111 22.9626 24.5884 19.6359 30.2728 18.0887 25.7372 22.7355 27.3758
Translational Efficiency 0.3661 0.3402 0.3736 0.2052 0.5262 0.3172 0.3981 0.3013 0.4935 0.4466

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1712 1411 140 172 1008 2161 1315 1457 2720 3572 1455 1629

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.37 746.99 1034.23 887.70 680.79 785.92 863.80 754.37 658.50 770.54 880.20 768.45
Standard Deviation 86.41 113.30 129.88 118.65 90.70 94.80 132.83 104.78 89.67 104.26 141.75 113.95
Intensity Change Log 2 — 0.210962 0.680359 0.459946 — 0.207173 0.343487 0.148062 — 0.209018 0.517248 0.308261

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000287 0.001213 0.002595 0.001566 0.000350 0.000344 0.001502 0.001003 0.000310 0.000687 0.001607 0.001063
Bud Neck 0.003229 0.011485 0.011597 0.020039 0.002451 0.006879 0.011531 0.017918 0.002941 0.008698 0.011538 0.018142
Bud Site 0.001253 0.005827 0.002454 0.009855 0.000639 0.001580 0.001778 0.003766 0.001026 0.003258 0.001843 0.004409
Cell Periphery 0.000171 0.000185 0.000105 0.000185 0.000123 0.000108 0.000087 0.000138 0.000153 0.000138 0.000089 0.000143
Cytoplasm 0.039039 0.035279 0.004684 0.035441 0.019423 0.012405 0.005864 0.015857 0.031769 0.021441 0.005751 0.017925
Cytoplasmic Foci 0.012898 0.010003 0.000137 0.003725 0.005018 0.003139 0.000197 0.001225 0.009978 0.005850 0.000191 0.001489
Eisosomes 0.000042 0.000053 0.000064 0.000057 0.000036 0.000028 0.000033 0.000037 0.000040 0.000038 0.000036 0.000039
Endoplasmic Reticulum 0.002433 0.005226 0.011549 0.012654 0.002152 0.002070 0.007058 0.005961 0.002329 0.003317 0.007491 0.006668
Endosome 0.001002 0.002837 0.000618 0.005923 0.000774 0.001123 0.000603 0.001726 0.000918 0.001800 0.000604 0.002169
Golgi 0.001650 0.002643 0.000228 0.001421 0.000580 0.001497 0.000197 0.001385 0.001254 0.001950 0.000200 0.001388
Lipid Particles 0.003997 0.002535 0.000621 0.000867 0.001752 0.001424 0.000390 0.000625 0.003165 0.001863 0.000412 0.000650
Mitochondria 0.008237 0.011718 0.002001 0.001987 0.002889 0.005478 0.002416 0.003763 0.006255 0.007943 0.002376 0.003575
Mitotic Spindle 0.002597 0.007420 0.001352 0.007467 0.000921 0.005267 0.003326 0.011832 0.001976 0.006118 0.003136 0.011371
None 0.052119 0.028018 0.006828 0.014547 0.021410 0.013143 0.004766 0.009698 0.040739 0.019019 0.004964 0.010210
Nuclear Periphery 0.001284 0.001005 0.000815 0.001630 0.000501 0.000860 0.000803 0.001542 0.000994 0.000917 0.000805 0.001552
Nuclear Periphery Foci 0.001999 0.002996 0.000721 0.000974 0.001124 0.000831 0.001029 0.002765 0.001675 0.001686 0.000999 0.002576
Nucleolus 0.075044 0.076644 0.022786 0.007072 0.070122 0.065795 0.024656 0.049492 0.073220 0.070080 0.024476 0.045013
Nucleus 0.788400* 0.784193* 0.924394* 0.856639* 0.866766* 0.873932* 0.928745* 0.865046* 0.817442* 0.838484* 0.928327* 0.864159*
Peroxisomes 0.002443 0.003725 0.000714 0.000739 0.001375 0.001685 0.000484 0.000588 0.002047 0.002491 0.000506 0.000604
Vacuole 0.001156 0.004523 0.005466 0.016479 0.001177 0.001357 0.004213 0.003818 0.001164 0.002607 0.004333 0.005155
Vacuole Periphery 0.000719 0.002471 0.000269 0.000732 0.000417 0.001056 0.000321 0.001815 0.000607 0.001615 0.000316 0.001701

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.25 -9.60 -7.79 -3.65 2.82 0.26 -17.36 -11.11 -11.38 6.07 -4.92 -21.69 -15.18 -6.42 6.43
Bud Neck -9.36 -6.68 -5.94 -3.74 -2.06 -8.27 -14.42 -15.81 -11.99 -4.29 -11.94 -15.21 -16.63 -11.52 -4.71
Bud Site -4.85 -5.52 -3.94 -1.58 -2.36 -3.05 -6.78 -6.31 -4.44 -3.65 -5.24 -6.09 -6.68 -2.74 -4.26
Cell Periphery -0.65 1.55 -0.35 0.17 -1.56 0.46 1.35 -0.26 -1.06 -3.40 0.84 4.50 0.90 0.04 -3.69
Cytoplasm 1.29 11.79 1.10 0.42 -4.00 2.72 5.86 1.53 -1.56 -6.21 4.93 14.35 6.50 2.01 -7.31
Cytoplasmic Foci 2.46 9.89 7.21 5.37 -1.82 2.66 5.44 4.47 4.15 -4.01 4.98 11.28 9.90 8.04 -4.41
Eisosomes -2.95 -3.57 -5.30 -4.09 -0.02 3.45 1.40 -1.32 -6.17 -3.63 1.89 1.16 -2.17 -3.91 -3.08
Endoplasmic Reticulum -7.95 -8.69 -9.67 -7.56 -1.55 0.44 -14.79 -11.80 -13.55 1.99 -5.18 -17.75 -15.07 -12.10 0.94
Endosome -3.94 -0.15 -1.59 -0.34 -1.55 -0.68 1.18 -1.79 -1.22 -3.00 -3.19 2.33 -2.28 0.08 -3.39
Golgi -1.59 4.46 2.27 3.57 -0.81 -1.76 3.10 -0.84 0.66 -1.96 -1.44 5.52 0.84 1.83 -2.04
Lipid Particles 2.88 6.42 6.62 3.90 -0.37 1.65 5.75 4.62 4.25 -2.27 4.31 10.11 8.88 6.41 -2.34
Mitochondria -1.98 4.79 5.62 6.94 0.71 -2.47 0.42 -0.52 2.30 -1.24 -1.66 4.52 3.23 5.38 -1.17
Mitotic Spindle -3.37 -1.48 -3.14 -2.16 -2.23 -3.36 -3.27 -5.69 -3.58 -3.64 -4.22 -2.75 -5.92 -3.77 -4.12
None 6.84 14.14 10.59 5.00 -2.08 3.91 8.97 6.29 3.28 -4.66 9.74 18.21 14.90 7.36 -5.11
Nuclear Periphery 0.89 -0.26 -3.61 -5.11 -3.94 -3.68 -6.56 -6.34 -4.46 -3.06 0.38 -0.85 -3.50 -5.31 -3.92
Nuclear Periphery Foci -1.15 0.50 -0.30 0.71 -0.64 1.22 0.42 -1.89 -2.81 -2.31 0.56 2.21 -1.16 -1.48 -2.40
Nucleolus 0.04 10.02 19.70 19.65 2.61 0.86 10.44 3.45 3.19 -7.69 1.25 16.33 7.05 6.43 -7.06
Nucleus -0.26 -9.81 -2.81 -2.63 3.99 -1.41 -7.97 1.34 3.15 10.64 -4.34 -18.58 -5.44 -1.76 11.39
Peroxisomes -1.28 4.95 5.57 5.19 1.29 0.02 3.87 3.39 4.14 -2.42 -0.25 7.39 6.86 6.74 -1.93
Vacuole -5.15 -3.03 -4.66 -4.11 -2.56 -0.28 -3.06 -5.02 -5.09 -1.06 -3.88 -4.15 -7.10 -5.54 -2.33
Vacuole Periphery -3.71 2.37 1.22 4.27 -0.82 -1.76 0.98 -2.09 -0.67 -2.33 -3.45 2.50 -1.67 0.52 -2.35
Standard name
Human Ortholog
Description Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
Localization
Cell Percentages nucleus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Snf11

Snf11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snf11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available