Standard name
Human Ortholog
Description Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.09 0.09 0.1 0.16 0.12 0.11 0.1 0.09 0.08 0.1 0.13 0.17 0.23 0.29 0.13 0.17 0.14 0 0.06 0 0.09 0.06 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.19 0.13 0.12 0.08 0.59 0.67 0.66 0.71 0.68 0.75 0.68 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Nucleus 0.82 0.74 0.72 0.78 0.63 0.51 0.48 0.52 0.44 0.43 0.43 0.37 0.76 0.74 0.66 0.68 0.72 0.7 0.82 0.69 0.72 0.53 0.54 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.1 0.15 0.1 0.19 0.11 0.08 0.07 0.16 0.1 0.09 0.17 0.09 0.06 0.11 0.23 0.11 0.23 0 0.14 0.16 0.25 0.29 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 4 5 5 7 7 6 1 0 0 0 0 0 0 0 2 3 1 3 5
Bud Neck 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 1 0 0 1 5
Bud Site 0 0 0 0 0 1 8 6 9 14 17 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 4 27 16 19 44 46 44 55 27 28 41 52 20 45 51 12 22 16 2 19 8 12 18 15
Endoplasmic Reticulum 0 1 0 1 0 0 1 0 1 0 0 1 2 0 1 7 7 6 0 0 1 0 1 1
Endosome 0 3 4 0 17 2 1 0 2 3 1 5 0 1 1 0 2 0 0 5 1 0 4 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 1 1 0 0
Mitochondria 7 56 24 24 23 225 266 351 214 248 306 281 3 0 0 5 9 1 2 10 2 6 5 8
Nucleus 31 220 133 155 172 194 188 280 132 159 176 153 88 144 116 62 94 80 58 234 144 76 174 125
Nuclear Periphery 0 0 0 2 0 1 2 5 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 3 3 3 1 2 0 0 0 0 0 0 0 0 0 0 0 0 3 4
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 2 2 1 0 1 0 1 1 0 1 0 0 1 0 1 7 0 4 10 8
Vac/Vac Membrane 7 29 27 19 51 42 30 37 47 37 38 71 10 12 20 21 14 26 2 48 33 36 95 79
Unique Cell Count 38 298 184 200 271 381 395 535 301 366 407 411 116 194 176 91 131 114 71 339 201 145 324 266
Labelled Cell Count 50 337 206 220 310 520 549 743 442 499 588 571 123 203 190 107 150 131 71 339 201 145 324 266


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.6 4.6 4.7 5.3 3.7 3.7 3.9 3.5 3.7 3.5 3.5 5.3 5.4 5.6 6.4 6.4 6.5 4.8 5.1 5.2
Std Deviation (1e-4) 0.5 0.9 0.9 1.8 4.2 1.8 2.1 1.1 0.6 1.1 1.0 1.1 1.1 1.0 1.1 1.1 1.2 1.3 0.8 1.1 1.0
Intensity Change (Log2) – – – 0.05 0.22 -0.3 -0.29 -0.24 -0.4 -0.31 -0.39 -0.38 0.22 0.23 0.29 0.49 0.49 0.5 0.07 0.15 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.7 -1.0 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6946 -0.4683 -0.3496 -0.421 -0.8979 -0.3496 0.9193 1.367 1.2724 1.0793 0.9761 1.1613 -0.6606 0.0997 -0.5378 -0.2891 -0.5357 -0.0934
Actin 0.0109 0.0004 0.0071 0.0005 0.0259 0.0071 0.0191 0.0001 0.0032 0.0079 0.0001 0.0004 0.0035 0.0005 0 0.0003 0.0015 0.0001
Bud 0.0002 0.0002 0.0004 0.0001 0.0003 0.0002 0.0005 0.0001 0.0005 0.0012 0.0003 0.0001 0.0016 0.0001 0 0.0001 0 0.0001
Bud Neck 0.0009 0.0004 0.0007 0.0007 0.0007 0.0015 0.0022 0.0003 0.0011 0.0003 0.0011 0.0011 0.0027 0.0006 0.0001 0.0003 0.0004 0.0015
Bud Periphery 0.0005 0.0002 0.0004 0.0001 0.0003 0.0004 0.0005 0 0.0012 0.0008 0.0001 0.0001 0.0096 0.0001 0 0.0001 0 0.0001
Bud Site 0.0008 0.0018 0.0042 0.0015 0.0023 0.0004 0.0074 0.0006 0.0027 0.0014 0.0028 0.0001 0.0029 0.0032 0.0001 0.0002 0.0002 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0 0.0001 0.0003 0 0.0003 0 0 0 0.0035 0.0001 0 0 0 0
Cytoplasm 0.053 0.0305 0.0236 0.0159 0.0352 0.0211 0.0427 0.0352 0.0159 0.0117 0.0128 0.0142 0.021 0.0723 0.007 0.041 0.0479 0.0347
Cytoplasmic Foci 0.0133 0.001 0.0071 0.0238 0.0055 0.0012 0.0223 0.0005 0.0055 0.0029 0.0396 0.0004 0.025 0.0017 0.0003 0.0009 0.0087 0.0003
Eisosomes 0.0002 0 0.0002 0 0.0002 0.0001 0.0008 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0029 0.0015 0.0011 0.0006 0.0009 0.0012 0.0032 0.0008 0.0009 0.0009 0.0002 0.0034 0.0009 0.0027 0.0001 0.0022 0.0011 0.0004
Endosome 0.0089 0.0022 0.0052 0.002 0.0019 0.006 0.0175 0.0008 0.0121 0.0046 0.0151 0.0018 0.0032 0.0024 0.0002 0.0019 0.0056 0.0004
Golgi 0.0031 0.0001 0.0013 0.0007 0.0026 0.0031 0.0149 0 0.0025 0.0014 0.0026 0.0006 0.0013 0.0001 0 0 0.0122 0
Lipid Particles 0.0092 0.0003 0.0042 0.0067 0.0044 0.0021 0.0136 0 0.009 0.0065 0.0012 0.0056 0.0052 0.0002 0 0.0001 0.1 0
Mitochondria 0.0017 0.0013 0.0027 0.0007 0.003 0.0073 0.0093 0.0004 0.0075 0.0065 0.0005 0.0028 0.0107 0.0005 0.0002 0.0006 0.0024 0.0003
None 0.0673 0.0629 0.0705 0.0243 0.0142 0.0638 0.0415 0.0187 0.0357 0.01 0.0036 0.0016 0.056 0.124 0.0771 0.006 0.1049 0.0108
Nuclear Periphery 0.0266 0.0243 0.0313 0.0301 0.0128 0.0126 0.0116 0.0181 0.0071 0.0106 0.0046 0.0097 0.0125 0.0179 0.0032 0.0378 0.0087 0.0032
Nucleolus 0.004 0.0049 0.0038 0.0064 0.0085 0.0085 0.0036 0.0037 0.0045 0.003 0.007 0.0065 0.0041 0.0053 0.0024 0.0026 0.0043 0.0042
Nucleus 0.7633 0.8527 0.8123 0.8263 0.8424 0.8442 0.7528 0.901 0.8537 0.9003 0.8843 0.9416 0.8007 0.7072 0.9074 0.8859 0.666 0.9355
Peroxisomes 0.0044 0.0004 0.0025 0.0022 0.0091 0.0006 0.0133 0.0001 0.0113 0.0178 0.0045 0.0005 0.0119 0.0001 0 0 0.0139 0
Punctate Nuclear 0.0243 0.013 0.0199 0.0563 0.0291 0.0165 0.0208 0.0188 0.0164 0.0095 0.0183 0.009 0.0095 0.059 0.0015 0.0188 0.021 0.0078
Vacuole 0.0032 0.0015 0.0011 0.0005 0.0007 0.0013 0.0017 0.0006 0.0073 0.0013 0.0011 0.0004 0.0105 0.0018 0.0002 0.0007 0.0006 0.0004
Vacuole Periphery 0.0012 0.0005 0.0004 0.0003 0.0003 0.0009 0.0005 0.0002 0.0015 0.0009 0.0001 0.0003 0.0034 0.0003 0.0001 0.0004 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0369 4.8471 3.5967 5.1555 7.03 5.4191 4.084 3.0321 4.0288 7.5911
Translational Efficiency 0.501 0.7013 0.6576 0.5329 0.4095 0.7869 0.6179 0.6993 0.5357 0.56

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2130 2115 3272 82 2352 1356 1557 37 4482 3471 4829 119

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 704.11 793.24 844.09 914.65 716.48 788.36 854.04 977.07 710.60 791.33 847.30 934.06
Standard Deviation 81.60 106.64 99.83 110.58 92.86 108.82 96.66 129.70 87.90 107.52 98.93 120.38
Intensity Change Log 2 — 0.171957 0.261596 0.377419 — 0.137928 0.253377 0.447535 — 0.154895 0.257457 0.413208

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.000106 0.000161 0.000392 0.000043 0.001828 0.000261 0.002788 0.000045 0.000779 0.000194 0.001137
Bud Neck 0.002415 0.004328 0.010543 0.001964 0.002066 0.021473 0.006124 0.024037 0.002232 0.011026 0.009118 0.008827
Bud Site 0.001681 0.000871 0.004960 0.005212 0.000592 0.025626 0.004994 0.242862* 0.001110 0.010542 0.004971 0.079103
Cell Periphery 0.000096 0.000062 0.000162 0.000294 0.000097 0.000523 0.000070 0.000440 0.000096 0.000242 0.000133 0.000340
Cytoplasm 0.137285* 0.051181 0.034376 0.140969* 0.104478* 0.106802* 0.016732 0.132372* 0.120069* 0.072911 0.028688 0.138296*
Cytoplasmic Foci 0.014324 0.003818 0.000443 0.007994 0.008116 0.031314 0.000188 0.021673 0.011066 0.014560 0.000361 0.012247
Eisosomes 0.000018 0.000011 0.000014 0.000066 0.000015 0.000076 0.000015 0.000070 0.000016 0.000037 0.000014 0.000067
Endoplasmic Reticulum 0.000992 0.002251 0.002472 0.007673 0.000995 0.008308 0.003555 0.004862 0.000994 0.004617 0.002821 0.006799
Endosome 0.000951 0.000627 0.000298 0.000998 0.000454 0.007106 0.000292 0.029126 0.000690 0.003158 0.000296 0.009744
Golgi 0.000182 0.000166 0.000025 0.000082 0.000096 0.004573 0.000024 0.006845 0.000137 0.001888 0.000025 0.002185
Lipid Particles 0.001654 0.001075 0.000133 0.001432 0.001050 0.004751 0.000220 0.001490 0.001337 0.002511 0.000161 0.001450
Mitochondria 0.002958 0.001900 0.001038 0.000539 0.001613 0.016074 0.000873 0.020951 0.002252 0.007438 0.000984 0.006886
Mitotic Spindle 0.000759 0.002799 0.001096 0.004380 0.000139 0.019277 0.002419 0.116669* 0.000434 0.009236 0.001522 0.039293
None 0.006673 0.005013 0.004670 0.013363 0.008163 0.007745 0.002618 0.002241 0.007455 0.006080 0.004009 0.009905
Nuclear Periphery 0.000965 0.001505 0.001071 0.001626 0.001050 0.003056 0.001054 0.002233 0.001009 0.002111 0.001066 0.001815
Nuclear Periphery Foci 0.000208 0.000474 0.000045 0.000619 0.000158 0.001078 0.000102 0.002394 0.000182 0.000710 0.000063 0.001171
Nucleolus 0.002167 0.002712 0.000881 0.001431 0.002745 0.007310 0.000817 0.005026 0.002471 0.004508 0.000860 0.002549
Nucleus 0.821896* 0.917648* 0.934048* 0.772414* 0.865421* 0.693190* 0.955857* 0.099704 0.844736* 0.829960* 0.941080* 0.563252*
Peroxisomes 0.001218 0.000747 0.000127 0.000266 0.000630 0.014154 0.000129 0.003442 0.000909 0.005985 0.000127 0.001253
Vacuole 0.003195 0.002254 0.003316 0.038134 0.001958 0.023767 0.003495 0.245831* 0.002546 0.010658 0.003374 0.102712
Vacuole Periphery 0.000315 0.000451 0.000120 0.000152 0.000119 0.001968 0.000162 0.034945 0.000212 0.001044 0.000133 0.010970

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.16 -6.16 -4.09 -3.74 -3.48 -5.14 -10.02 -4.84 -0.86 -4.22 -5.43 -10.82 -6.11 -2.36 -5.26
Bud Neck -2.77 -10.20 -2.21 -0.15 5.62 -12.65 -5.42 -3.19 1.30 -2.02 -11.81 -12.02 -3.77 1.81 1.16
Bud Site 1.35 -3.41 -2.05 -2.44 -0.65 -9.05 -3.25 -3.55 -2.88 -3.44 -8.32 -5.23 -3.49 -2.75 -3.20
Cell Periphery 3.26 -4.27 -1.53 -1.92 -0.86 -6.68 2.31 -2.01 1.76 -2.22 -5.68 -3.49 -2.31 -0.13 -1.84
Cytoplasm 18.10 24.12 1.46 -2.96 -3.94 -0.24 22.09 -1.24 -1.16 -5.60 13.17 31.21 0.33 -2.88 -5.97
Cytoplasmic Foci 8.02 12.62 4.48 -0.41 -2.48 -9.37 9.78 -1.10 3.43 -2.90 -2.85 15.73 1.78 3.27 -3.77
Eisosomes 5.03 4.00 -6.10 -6.59 -6.44 -15.07 -2.64 -5.05 -0.27 -4.77 -11.40 1.29 -7.76 -5.44 -7.88
Endoplasmic Reticulum -5.51 -7.76 -6.77 -6.07 -5.78 -13.80 -12.55 -3.08 -0.68 -2.00 -14.63 -14.17 -7.22 -4.80 -5.76
Endosome 1.36 3.45 -2.26 -3.57 -5.24 -8.16 1.73 -3.12 -1.81 -3.14 -7.17 3.95 -3.27 -1.80 -3.50
Golgi 0.08 3.02 1.05 1.00 -3.71 -5.68 2.25 -1.58 0.68 -1.62 -5.61 3.79 -1.51 1.22 -1.72
Lipid Particles 1.64 7.31 0.39 -1.20 -6.01 -9.62 5.94 -3.13 2.95 -4.64 -5.05 9.73 -2.21 1.96 -7.32
Mitochondria 1.16 2.23 2.81 3.36 3.48 -7.91 1.14 -1.70 0.89 -1.82 -5.86 2.43 -0.85 1.92 -1.58
Mitotic Spindle -2.38 -1.00 -1.60 -1.45 -1.57 -7.27 -2.89 -2.89 -2.28 -2.79 -7.59 -3.27 -3.13 -2.51 -3.03
None 1.75 2.89 -0.68 -1.31 -1.64 0.71 7.93 7.55 5.11 1.36 2.10 6.51 0.72 -0.13 -1.47
Nuclear Periphery -3.03 -1.93 -5.77 -3.93 -5.28 -10.63 -3.72 -1.57 -0.84 -1.45 -8.96 -3.50 -2.81 -1.55 -2.50
Nuclear Periphery Foci -2.49 4.47 -3.75 -2.37 -4.30 -4.43 0.73 -1.75 -1.44 -1.76 -4.97 3.27 -2.37 -1.80 -2.47
Nucleolus -1.36 3.02 2.20 3.74 -0.76 -4.89 4.45 -0.18 2.33 -1.40 -4.26 5.24 1.25 4.37 -1.57
Nucleus -15.11 -20.04 3.28 6.19 6.76 17.67 -15.45 15.02 10.84 16.98 3.05 -24.37 8.47 7.93 11.28
Peroxisomes 1.03 4.04 3.72 2.98 -1.28 -7.78 2.55 -1.76 6.59 -2.65 -7.17 4.65 0.67 7.28 -2.77
Vacuole 1.97 -1.21 -4.96 -5.00 -4.93 -13.60 -5.39 -7.33 -6.71 -7.22 -11.94 -5.08 -7.86 -7.38 -7.75
Vacuole Periphery -0.79 1.43 1.13 2.60 -1.24 -6.79 -0.59 -1.46 -1.29 -1.45 -5.77 1.20 -1.42 -1.18 -1.45
Standard name
Human Ortholog
Description Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
Localization
Cell Percentages nucleus (80%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad55

Rad55


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad55-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available