Standard name | |
---|---|
Human Ortholog | |
Description | Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 2 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 2 | 1 | 4 | 8 | 7 | 1 | 1 | 5 | 8 |
Bud | 7 | 1 | 23 | 15 | 6 | 13 | – | 32 | 19 | 11 | 9 | 17 | 1 | 1 | 1 | 1 | 0 | 2 | 3 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 4 | 0 | 0 | 3 | – | 10 | 9 | 10 | 5 | 15 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 11 | 9 | 3 | 4 | 2 | 2 | – | 4 | 6 | 2 | 2 | 3 | 4 | 5 | 5 | 2 | 3 | 7 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 29 | 31 | 26 | 16 | 23 | 15 | – | 30 | 14 | 12 | 10 | 9 | 7 | 9 | 13 | 5 | 10 | 15 | 6 | 4 | 2 | 0 | 1 | 2 |
Endoplasmic Reticulum | 11 | 5 | 4 | 2 | 1 | 2 | – | 2 | 2 | 6 | 1 | 0 | 8 | 9 | 3 | 3 | 4 | 2 | 1 | 3 | 1 | 1 | 0 | 1 |
Endosome | 16 | 18 | 1 | 1 | 39 | 4 | – | 8 | 1 | 1 | 0 | 0 | 7 | 9 | 20 | 4 | 4 | 3 | 32 | 18 | 18 | 6 | 15 | 17 |
Golgi | 6 | 15 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 38 | 30 | 15 | 13 | 6 | 19 | 11 | 11 | 5 | 5 | 25 | 16 |
Mitochondria | 329 | 146 | 249 | 252 | 200 | 383 | – | 834 | 518 | 489 | 352 | 445 | 141 | 134 | 78 | 4 | 6 | 12 | 337 | 215 | 208 | 77 | 143 | 148 |
Nucleus | 4 | 3 | 2 | 1 | 4 | 12 | – | 22 | 7 | 3 | 7 | 12 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 1 | 3 | 0 | 0 | 2 | 12 | – | 15 | 21 | 25 | 12 | 17 | 4 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 0 |
Nucleolus | 2 | 1 | 0 | 1 | 4 | 9 | – | 45 | 24 | 31 | 15 | 20 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 1 |
Peroxisomes | 1 | 5 | 1 | 4 | 17 | 2 | – | 24 | 0 | 3 | 0 | 2 | 1 | 0 | 10 | 1 | 0 | 2 | 8 | 2 | 8 | 4 | 11 | 7 |
SpindlePole | 3 | 4 | 7 | 2 | 8 | 8 | – | 27 | 10 | 8 | 4 | 14 | 1 | 5 | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 1 | 1 | 0 |
Vac/Vac Membrane | 65 | 65 | 14 | 5 | 32 | 33 | – | 57 | 33 | 16 | 16 | 15 | 28 | 27 | 15 | 7 | 8 | 20 | 30 | 21 | 11 | 2 | 10 | 31 |
Unique Cell Count | 404 | 230 | 272 | 271 | 257 | 409 | 924 | 549 | 521 | 366 | 463 | 183 | 181 | 128 | 37 | 35 | 72 | 462 | 299 | 270 | 105 | 226 | 246 | |
Labelled Cell Count | 492 | 308 | 336 | 305 | 338 | 498 | 1110 | 664 | 617 | 433 | 569 | 245 | 233 | 160 | 43 | 44 | 89 | 462 | 299 | 270 | 105 | 226 | 246 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 6.4 | 4.1 | 4.5 | 5.3 | 4.4 | – | 4.5 | 4.0 | 4.2 | 4.2 | 4.0 | 6.5 | 6.5 | 6.2 | 7.8 | 8.4 | 7.9 | 6.0 | 6.5 | 6.9 |
Std Deviation (1e-4) | 0.8 | 1.4 | 1.2 | 2.0 | 1.7 | 1.6 | – | 1.4 | 1.2 | 1.0 | 1.2 | 1.0 | 1.2 | 1.2 | 1.0 | 1.4 | 2.0 | 1.7 | 1.5 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.39 | 0.11 | – | 0.13 | -0.01 | 0.05 | 0.04 | -0.02 | 0.69 | 0.68 | 0.61 | 0.94 | 1.05 | 0.96 | 0.57 | 0.68 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -3.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | -3.7 | -3.7 | -3.0 | 0 | 0 | -1.7 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | -0.2 | -3.2 | – | -4.3 | -4.4 | -4.5 | -3.7 | -4.7 | -2.3 | -1.8 | 0.2 | 0 | 0 | 2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 |
Endosome | 0 | 6.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 7.9 | 6.9 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -4.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | -4.3 | -5.1 | -7.4 | -12.0 | -11.0 | -13.2 |
Nucleus | 0 | 0 | 2.0 | – | 1.7 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 4.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0.4 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.1 | 3.0 | 1.5 | – | 0.6 | 0.5 | -1.5 | -0.5 | -1.3 | 3.7 | 3.6 | 2.4 | 0 | 0 | 5.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7206 | 2.0402 | 1.8023 | 1.9345 | 1.3275 | 1.6852 | 2.0537 | 1.5204 | 1.7902 | 1.552 | 1.9004 | 1.5531 | 2.8046 | 2.3531 | 2.2806 | 2.5106 | 1.6555 | 2.7666 |
Actin | 0.0699 | 0.0131 | 0.0221 | 0.0183 | 0.07 | 0.0088 | 0.0524 | 0.0154 | 0.0294 | 0.0296 | 0.0563 | 0.0175 | 0.0457 | 0.0302 | 0.0347 | 0.0727 | 0.0309 | 0.0462 |
Bud | 0.001 | 0.0025 | 0.0015 | 0.0162 | 0.0017 | 0.0006 | 0.0012 | 0.0069 | 0.0024 | 0.0059 | 0.0018 | 0.0015 | 0.0011 | 0.0035 | 0.0017 | 0.0105 | 0.0022 | 0.001 |
Bud Neck | 0.0016 | 0.0029 | 0.0013 | 0.003 | 0.001 | 0.0067 | 0.0022 | 0.0012 | 0.0023 | 0.0006 | 0.0013 | 0.0161 | 0.0025 | 0.0015 | 0.0021 | 0.0011 | 0.0008 | 0.0024 |
Bud Periphery | 0.0017 | 0.0035 | 0.0027 | 0.0367 | 0.0044 | 0.0011 | 0.0025 | 0.01 | 0.0049 | 0.0241 | 0.0025 | 0.0038 | 0.0033 | 0.0047 | 0.0033 | 0.0243 | 0.0044 | 0.0028 |
Bud Site | 0.0018 | 0.0244 | 0.0061 | 0.018 | 0.0022 | 0.0015 | 0.006 | 0.0319 | 0.0121 | 0.0042 | 0.0038 | 0.0019 | 0.0026 | 0.0499 | 0.0222 | 0.0029 | 0.002 | 0.0024 |
Cell Periphery | 0.0007 | 0.0018 | 0.0007 | 0.0013 | 0.0028 | 0.0005 | 0.0015 | 0.0014 | 0.0006 | 0.0008 | 0.0004 | 0.0006 | 0.0017 | 0.0028 | 0.0011 | 0.0014 | 0.0007 | 0.0007 |
Cytoplasm | 0.024 | 0.0373 | 0.016 | 0.0221 | 0.0081 | 0.0065 | 0.0189 | 0.0624 | 0.0165 | 0.0202 | 0.0066 | 0.0167 | 0.0109 | 0.0345 | 0.0275 | 0.0308 | 0.0031 | 0.0066 |
Cytoplasmic Foci | 0.0551 | 0.061 | 0.0757 | 0.0892 | 0.0711 | 0.0461 | 0.0678 | 0.0673 | 0.0603 | 0.0451 | 0.0446 | 0.0415 | 0.0476 | 0.0719 | 0.0405 | 0.0264 | 0.0159 | 0.0302 |
Eisosomes | 0.0007 | 0.0005 | 0.0008 | 0.0002 | 0.0005 | 0.0003 | 0.0017 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0046 | 0.0015 | 0.0009 | 0.0006 | 0.0004 | 0.0006 |
Endoplasmic Reticulum | 0.0097 | 0.0098 | 0.0024 | 0.0013 | 0.0032 | 0.0053 | 0.0235 | 0.005 | 0.0023 | 0.0152 | 0.0056 | 0.0021 | 0.0148 | 0.005 | 0.0037 | 0.0006 | 0.0024 | 0.0186 |
Endosome | 0.1401 | 0.1628 | 0.1252 | 0.1022 | 0.138 | 0.1266 | 0.1961 | 0.1633 | 0.1329 | 0.1046 | 0.1497 | 0.1106 | 0.1047 | 0.1926 | 0.1117 | 0.027 | 0.098 | 0.1266 |
Golgi | 0.0944 | 0.1219 | 0.1236 | 0.0339 | 0.0678 | 0.087 | 0.1014 | 0.1217 | 0.1017 | 0.077 | 0.1162 | 0.0937 | 0.0755 | 0.1157 | 0.088 | 0.0316 | 0.0999 | 0.13 |
Lipid Particles | 0.0681 | 0.0259 | 0.0233 | 0.0275 | 0.011 | 0.0205 | 0.0659 | 0.0226 | 0.0202 | 0.0152 | 0.024 | 0.0415 | 0.049 | 0.0201 | 0.0136 | 0.0111 | 0.0022 | 0.0275 |
Mitochondria | 0.411 | 0.4646 | 0.4999 | 0.5542 | 0.5413 | 0.6298 | 0.3466 | 0.4387 | 0.5633 | 0.6025 | 0.4835 | 0.5952 | 0.4848 | 0.3842 | 0.5749 | 0.716 | 0.7128 | 0.5508 |
None | 0.0195 | 0.0126 | 0.017 | 0.0038 | 0.0013 | 0.0013 | 0.0085 | 0.0149 | 0.0144 | 0.0068 | 0.0746 | 0.0051 | 0.035 | 0.0223 | 0.0414 | 0.0006 | 0.0008 | 0.0025 |
Nuclear Periphery | 0.0244 | 0.005 | 0.0084 | 0.0012 | 0.0012 | 0.0066 | 0.0255 | 0.0014 | 0.0013 | 0.002 | 0.0017 | 0.0024 | 0.0218 | 0.0031 | 0.0005 | 0.0002 | 0.0004 | 0.0141 |
Nucleolus | 0.0011 | 0.0016 | 0.0008 | 0.001 | 0.0003 | 0.0003 | 0.001 | 0.0006 | 0.0004 | 0.001 | 0.0005 | 0.0054 | 0.0016 | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0.0016 |
Nucleus | 0.0119 | 0.002 | 0.0012 | 0.0037 | 0.0005 | 0.0011 | 0.0067 | 0.001 | 0.0008 | 0.0013 | 0.0007 | 0.0022 | 0.0233 | 0.0062 | 0.0004 | 0.0002 | 0.0003 | 0.0034 |
Peroxisomes | 0.0352 | 0.0227 | 0.0538 | 0.0286 | 0.056 | 0.0373 | 0.0427 | 0.0157 | 0.0179 | 0.0274 | 0.0165 | 0.0268 | 0.0495 | 0.0214 | 0.0175 | 0.0374 | 0.0107 | 0.0164 |
Punctate Nuclear | 0.0072 | 0.0016 | 0.0041 | 0.0057 | 0.0009 | 0.0009 | 0.0063 | 0.0005 | 0.001 | 0.0009 | 0.002 | 0.0008 | 0.0079 | 0.0011 | 0.0004 | 0.0003 | 0.0001 | 0.0023 |
Vacuole | 0.0119 | 0.0158 | 0.0075 | 0.0237 | 0.0094 | 0.0049 | 0.0119 | 0.0119 | 0.0089 | 0.0103 | 0.0038 | 0.0083 | 0.0065 | 0.0177 | 0.0072 | 0.0021 | 0.0055 | 0.0065 |
Vacuole Periphery | 0.009 | 0.0068 | 0.006 | 0.0082 | 0.0073 | 0.0061 | 0.0096 | 0.006 | 0.006 | 0.0049 | 0.0037 | 0.0058 | 0.0056 | 0.0092 | 0.0064 | 0.0021 | 0.0065 | 0.0068 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.3456 | 17.6139 | 28.3189 | 20.2098 | 8.5155 | 26.257 | 23.0038 | 27.0453 | 27.1891 | 27.0616 |
Translational Efficiency | 1.7887 | 1.3904 | 1.1753 | 1.9172 | 4.6763 | 1.6272 | 1.409 | 1.4294 | 1.6625 | 1.6534 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1199 | 1685 | 496 | 1207 | 1130 | 1702 | 1635 | 236 | 2329 | 3387 | 2131 | 1443 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 668.04 | 769.91 | 859.81 | 946.15 | 755.71 | 808.23 | 924.24 | 929.67 | 710.58 | 789.17 | 909.24 | 943.45 |
Standard Deviation | 106.94 | 119.63 | 121.18 | 143.56 | 89.57 | 110.16 | 103.83 | 162.42 | 108.17 | 116.55 | 111.49 | 146.93 |
Intensity Change Log 2 | — | 0.204755 | 0.364083 | 0.502134 | — | 0.096933 | 0.290435 | 0.298886 | — | 0.148529 | 0.325460 | 0.397826 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000904 | 0.001110 | 0.010029 | 0.011440 | 0.000817 | 0.001001 | 0.002248 | 0.012107 | 0.000862 | 0.001055 | 0.004059 | 0.011549 |
Bud Neck | 0.018291 | 0.006928 | 0.025448 | 0.010753 | 0.010669 | 0.007749 | 0.010860 | 0.042304 | 0.014593 | 0.007341 | 0.014255 | 0.015913 |
Bud Site | 0.017272 | 0.016129 | 0.113653* | 0.066580 | 0.012740 | 0.015424 | 0.047265 | 0.054120 | 0.015073 | 0.015775 | 0.062717 | 0.064543 |
Cell Periphery | 0.000658 | 0.000649 | 0.000334 | 0.000831 | 0.000966 | 0.000404 | 0.000186 | 0.001264 | 0.000808 | 0.000526 | 0.000220 | 0.000902 |
Cytoplasm | 0.022314 | 0.007303 | 0.106877* | 0.017040 | 0.010948 | 0.007079 | 0.021826 | 0.034319 | 0.016799 | 0.007191 | 0.041622 | 0.019866 |
Cytoplasmic Foci | 0.075721 | 0.043446 | 0.131158 | 0.073963 | 0.064358 | 0.052360 | 0.038894 | 0.130670 | 0.070208 | 0.047925 | 0.060369 | 0.083237 |
Eisosomes | 0.000283 | 0.000091 | 0.000164 | 0.000136 | 0.000160 | 0.000099 | 0.000057 | 0.000251 | 0.000224 | 0.000095 | 0.000082 | 0.000155 |
Endoplasmic Reticulum | 0.001855 | 0.000599 | 0.012540 | 0.002477 | 0.001213 | 0.000374 | 0.001047 | 0.001500 | 0.001544 | 0.000486 | 0.003722 | 0.002317 |
Endosome | 0.078980 | 0.085008 | 0.063973 | 0.094281 | 0.076315 | 0.084995 | 0.065069 | 0.107950 | 0.077687 | 0.085002 | 0.064814 | 0.096517 |
Golgi | 0.099353 | 0.129525* | 0.043111 | 0.140655* | 0.119609* | 0.131459* | 0.058351 | 0.113799* | 0.109181 | 0.130497* | 0.054804 | 0.136263* |
Lipid Particles | 0.017008 | 0.003140 | 0.008236 | 0.004231 | 0.007612 | 0.002088 | 0.000293 | 0.033295 | 0.012449 | 0.002611 | 0.002142 | 0.008984 |
Mitochondria | 0.580903* | 0.629686* | 0.100680* | 0.372577* | 0.616506* | 0.617111* | 0.541993* | 0.245689* | 0.598177* | 0.623367* | 0.439275* | 0.351824* |
Mitotic Spindle | 0.004225 | 0.010837 | 0.101106* | 0.056035 | 0.005976 | 0.010403 | 0.060603 | 0.050628 | 0.005075 | 0.010619 | 0.070030 | 0.055151 |
None | 0.004413 | 0.000520 | 0.000968 | 0.000720 | 0.000436 | 0.000385 | 0.000330 | 0.003076 | 0.002483 | 0.000452 | 0.000479 | 0.001105 |
Nuclear Periphery | 0.000414 | 0.000092 | 0.001364 | 0.000388 | 0.000221 | 0.000072 | 0.000222 | 0.000713 | 0.000320 | 0.000082 | 0.000488 | 0.000441 |
Nuclear Periphery Foci | 0.000643 | 0.000251 | 0.003375 | 0.000746 | 0.000197 | 0.000125 | 0.000140 | 0.001366 | 0.000427 | 0.000188 | 0.000893 | 0.000847 |
Nucleolus | 0.002350 | 0.001254 | 0.004697 | 0.001670 | 0.001164 | 0.000489 | 0.000477 | 0.007145 | 0.001775 | 0.000870 | 0.001459 | 0.002566 |
Nucleus | 0.007319 | 0.003445 | 0.076395 | 0.004336 | 0.002799 | 0.000859 | 0.002441 | 0.018292 | 0.005126 | 0.002146 | 0.019654 | 0.006618 |
Peroxisomes | 0.030965 | 0.023625 | 0.060877 | 0.029537 | 0.032525 | 0.025791 | 0.015783 | 0.063543 | 0.031722 | 0.024714 | 0.026279 | 0.035099 |
Vacuole | 0.018515 | 0.017860 | 0.115195 | 0.070068 | 0.013877 | 0.020748 | 0.096419 | 0.049843 | 0.016265 | 0.019311 | 0.100789 | 0.066760 |
Vacuole Periphery | 0.017612 | 0.018501 | 0.019820 | 0.041537 | 0.020893 | 0.020983 | 0.035496 | 0.028126 | 0.019204 | 0.019749 | 0.031847 | 0.039343 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.69 | -5.10 | -5.91 | -5.84 | -0.68 | -0.72 | -3.26 | -2.92 | -2.87 | -2.53 | -0.97 | -5.79 | -6.64 | -6.52 | -4.46 |
Bud Neck | 4.85 | -2.44 | 2.93 | -2.47 | 5.56 | 1.64 | -0.35 | -3.68 | -4.05 | -3.64 | 4.89 | -0.06 | -0.67 | -4.55 | -0.68 |
Bud Site | 0.42 | -11.79 | -11.22 | -12.29 | 4.93 | -1.34 | -12.73 | -4.93 | -4.60 | -0.70 | -0.46 | -16.27 | -13.27 | -13.38 | -0.71 |
Cell Periphery | 0.06 | 4.55 | -1.08 | -0.85 | -3.57 | 3.93 | 5.61 | -0.79 | -2.55 | -3.23 | 2.58 | 7.67 | -0.59 | -2.46 | -5.27 |
Cytoplasm | 5.62 | -10.57 | 1.69 | -5.17 | 11.43 | 2.37 | -5.73 | -3.85 | -4.53 | -1.85 | 6.06 | -9.63 | -1.62 | -7.24 | 7.98 |
Cytoplasmic Foci | 8.64 | -7.48 | 0.46 | -8.42 | 7.87 | 3.39 | 7.55 | -6.43 | -7.69 | -9.02 | 8.61 | 3.51 | -3.44 | -10.36 | -6.32 |
Eisosomes | 2.87 | 1.72 | 2.17 | -3.53 | 2.08 | 4.83 | 8.79 | -3.24 | -5.74 | -7.40 | 3.67 | 4.01 | 1.88 | -5.19 | -6.26 |
Endoplasmic Reticulum | 2.44 | -6.64 | -1.10 | -5.55 | 6.43 | 3.28 | 0.43 | -0.75 | -3.93 | -1.22 | 3.63 | -4.58 | -2.04 | -6.69 | 3.06 |
Endosome | -1.23 | 3.40 | -2.94 | -1.87 | -5.99 | -1.79 | 2.52 | -3.49 | -2.56 | -5.00 | -2.13 | 4.07 | -4.40 | -2.79 | -8.00 |
Golgi | -4.90 | 10.23 | -5.59 | -1.41 | -14.57 | -1.75 | 10.51 | 0.59 | 1.58 | -4.65 | -4.67 | 13.55 | -4.23 | -0.77 | -14.54 |
Lipid Particles | 5.96 | 3.32 | 5.28 | -1.06 | 2.89 | 4.88 | 6.80 | -3.51 | -4.30 | -4.54 | 7.46 | 7.92 | 1.70 | -4.37 | -4.69 |
Mitochondria | -3.83 | 37.74* | 14.93 | 20.15 | -21.59* | -0.06 | 6.14 | 17.01 | 17.61 | 13.69 | -2.82 | 15.52 | 21.82 | 25.69 | 6.98 |
Mitotic Spindle | -4.70 | -11.13 | -13.82 | -11.63 | 4.54 | -3.13 | -15.73 | -5.67 | -5.09 | 1.24 | -5.58 | -19.48 | -14.80 | -12.95 | 3.09 |
None | 3.48 | 3.04 | 3.26 | -1.11 | 1.28 | 0.24 | 1.37 | -2.41 | -2.44 | -2.52 | 3.48 | 3.46 | 2.20 | -2.73 | -2.73 |
Nuclear Periphery | 2.82 | -5.46 | 0.17 | -5.13 | 6.35 | 3.04 | -0.25 | -2.43 | -3.25 | -2.41 | 3.76 | -2.78 | -1.50 | -6.37 | 1.23 |
Nuclear Periphery Foci | 1.96 | -5.56 | -0.54 | -3.43 | 5.49 | 2.35 | 1.38 | -2.37 | -2.52 | -2.46 | 2.30 | -3.44 | -2.62 | -4.76 | 0.52 |
Nucleolus | 1.63 | -2.00 | 0.85 | -0.89 | 2.77 | 1.19 | 1.22 | -2.75 | -3.11 | -3.12 | 2.05 | 0.65 | -1.33 | -3.23 | -2.07 |
Nucleus | 1.96 | -10.17 | 1.54 | -0.71 | 10.68 | 2.56 | 0.00 | -3.93 | -4.47 | -3.95 | 2.77 | -7.94 | -1.21 | -4.02 | 7.10 |
Peroxisomes | 2.41 | -4.86 | 0.40 | -1.87 | 5.06 | 2.11 | 5.67 | -4.06 | -5.09 | -6.51 | 3.18 | 2.38 | -1.09 | -3.68 | -3.05 |
Vacuole | 0.32 | -14.71 | -12.84 | -13.49 | 6.81 | -3.76 | -20.39 | -5.90 | -4.78 | 6.80 | -2.20 | -24.49 | -14.88 | -14.18 | 7.82 |
Vacuole Periphery | -0.60 | -0.17 | -9.13 | -8.70 | -7.15 | -0.05 | -6.11 | -1.45 | -1.46 | 1.53 | -0.48 | -6.77 | -8.69 | -8.56 | -3.01 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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