Standard name | |
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Human Ortholog | |
Description | Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 2 | – | 5 | 5 | 0 | 0 | 0 | – | – | 0 | 0 | 5 | 2 | 2 | 15 | 2 |
Bud Neck | 0 | 0 | 6 | 0 | 2 | 1 | 1 | 3 | 0 | – | 3 | 2 | 0 | 0 | 0 | – | – | 0 | 0 | 1 | 0 | 0 | 4 | 5 |
Bud Site | 1 | 0 | 0 | 1 | 1 | 2 | 5 | 1 | 14 | – | 14 | 18 | 1 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 11 | 25 | 14 | 26 | 13 | 17 | 27 | 12 | – | 8 | 3 | 14 | 30 | 40 | – | – | 2 | 2 | 1 | 3 | 1 | 4 | 2 |
Endoplasmic Reticulum | 0 | 2 | 2 | 0 | 3 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 1 | 1 | – | – | 1 | 1 | 0 | 1 | 0 | 0 | 2 |
Endosome | 0 | 0 | 5 | 1 | 4 | 2 | 0 | 2 | 1 | – | 0 | 1 | 1 | 2 | 1 | – | – | 0 | 3 | 3 | 2 | 1 | 2 | 6 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 2 | 1 | 1 | 1 | 1 |
Mitochondria | 30 | 59 | 5 | 22 | 9 | 82 | 113 | 41 | 166 | – | 160 | 88 | 29 | 7 | 13 | – | – | 4 | 1 | 7 | 6 | 1 | 5 | 6 |
Nucleus | 112 | 280 | 207 | 231 | 239 | 186 | 287 | 281 | 261 | – | 246 | 216 | 243 | 274 | 227 | – | – | 14 | 109 | 281 | 215 | 135 | 217 | 177 |
Nuclear Periphery | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 4 | – | 1 | 2 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 11 | 1 | 2 | 0 | 1 | 3 | 3 | 4 | 24 | – | 37 | 34 | 25 | 7 | 18 | – | – | 0 | 6 | 9 | 11 | 8 | 18 | 25 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 3 | 19 | 13 | 8 | 10 | 5 | 10 | 6 | – | 6 | 11 | 3 | 2 | 0 | – | – | 0 | 2 | 5 | 11 | 1 | 8 | 8 |
Vac/Vac Membrane | 2 | 10 | 4 | 0 | 6 | 5 | 1 | 3 | 3 | – | 3 | 1 | 4 | 2 | 12 | – | – | 4 | 5 | 25 | 25 | 28 | 50 | 38 |
Unique Cell Count | 129 | 318 | 253 | 253 | 282 | 243 | 340 | 337 | 320 | 315 | 260 | 280 | 318 | 293 | 19 | 135 | 346 | 288 | 185 | 339 | 287 | |||
Labelled Cell Count | 166 | 369 | 277 | 282 | 299 | 306 | 437 | 376 | 494 | 483 | 382 | 320 | 327 | 312 | 25 | 135 | 346 | 288 | 185 | 339 | 287 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.8 | 5.6 | 5.1 | 5.9 | 4.5 | 4.7 | 5.3 | 4.4 | – | 4.1 | 4.5 | 5.3 | 5.8 | 5.7 | – | – | 7.6 | 4.9 | 5.1 | 5.6 |
Std Deviation (1e-4) | 1.2 | 0.7 | 1.3 | 1.4 | 1.5 | 1.1 | 1.1 | 1.4 | 1.3 | – | 1.1 | 1.0 | 1.4 | 1.6 | 1.6 | – | – | 1.8 | 1.6 | 1.8 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.15 | 0.06 | -0.32 | -0.25 | -0.09 | -0.36 | – | -0.47 | -0.32 | -0.09 | 0.04 | 0.01 | – | – | 0.44 | -0.19 | -0.14 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8684 | 3.041 | 3.0259 | 3.5202 | 2.246 | 2.9077 | 0.8941 | 1.6308 | 1.2977 | 1.431 | 0.6482 | 1.2294 | 1.795 | 2.0795 | 1.9443 | 1.9453 | 1.5315 | 2.0643 |
Actin | 0.0078 | 0 | 0.0024 | 0.0011 | 0.0254 | 0.0078 | 0.0011 | 0 | 0.003 | 0.0217 | 0.0027 | 0.0001 | 0.0039 | 0 | 0.0002 | 0 | 0.0002 | 0 |
Bud | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Bud Neck | 0.0014 | 0.0001 | 0.0004 | 0.0035 | 0.0023 | 0.0022 | 0.0011 | 0.0001 | 0.0012 | 0.0009 | 0.0004 | 0.0008 | 0.0014 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0006 |
Bud Periphery | 0.0004 | 0 | 0.0006 | 0.0003 | 0.0001 | 0.0007 | 0.0019 | 0 | 0.0005 | 0.0006 | 0.0009 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Bud Site | 0.0009 | 0 | 0.0033 | 0.0009 | 0.0002 | 0.0007 | 0.0016 | 0.0001 | 0.0072 | 0.0029 | 0.0006 | 0.0002 | 0.0008 | 0 | 0.0028 | 0 | 0.0004 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0037 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 |
Cytoplasm | 0.007 | 0.0008 | 0.0004 | 0.0016 | 0.0004 | 0.0003 | 0.0036 | 0.0003 | 0.0008 | 0.0009 | 0.0002 | 0.0006 | 0.0012 | 0.0001 | 0.0018 | 0 | 0.0015 | 0.0001 |
Cytoplasmic Foci | 0.0068 | 0 | 0.0002 | 0.0012 | 0.0048 | 0.0037 | 0.0073 | 0 | 0.0079 | 0.0032 | 0.0011 | 0.0035 | 0.0031 | 0 | 0.0019 | 0 | 0.0052 | 0.0032 |
Eisosomes | 0.0005 | 0 | 0.0027 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0034 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.001 | 0 | 0.0003 | 0.0006 | 0.0005 | 0.0001 | 0.001 | 0 | 0.0007 | 0 | 0.0006 | 0 |
Endosome | 0.0142 | 0 | 0.0002 | 0.0004 | 0.0015 | 0.0048 | 0.0173 | 0 | 0.0063 | 0.0172 | 0.0028 | 0.0025 | 0.0027 | 0 | 0.0046 | 0 | 0.0155 | 0.0001 |
Golgi | 0.0045 | 0 | 0.0002 | 0.0008 | 0.0016 | 0.006 | 0.0071 | 0 | 0.0047 | 0.0135 | 0.0027 | 0.001 | 0.001 | 0 | 0.0003 | 0 | 0.0094 | 0 |
Lipid Particles | 0.0232 | 0 | 0.0002 | 0.0083 | 0.0052 | 0.0111 | 0.0104 | 0 | 0.0016 | 0.0021 | 0.0058 | 0.0024 | 0.0031 | 0 | 0.0016 | 0 | 0.0074 | 0.0007 |
Mitochondria | 0.0066 | 0.0001 | 0.0039 | 0.0013 | 0.0007 | 0.0156 | 0.0113 | 0.0001 | 0.008 | 0.0105 | 0.0286 | 0.0024 | 0.0015 | 0 | 0.0008 | 0 | 0.004 | 0 |
None | 0.0281 | 0.0006 | 0.0009 | 0.0002 | 0.0017 | 0.0002 | 0.0009 | 0.0037 | 0.0001 | 0.001 | 0.0002 | 0.0004 | 0.0017 | 0 | 0.0081 | 0 | 0.0109 | 0 |
Nuclear Periphery | 0.028 | 0.0026 | 0.0006 | 0.0042 | 0.0169 | 0.0013 | 0.0096 | 0.0012 | 0.0076 | 0.0055 | 0.0052 | 0.0015 | 0.0051 | 0.0007 | 0.0037 | 0.0002 | 0.0029 | 0.0009 |
Nucleolus | 0.0304 | 0.0163 | 0.0137 | 0.0108 | 0.0933 | 0.051 | 0.0222 | 0.0174 | 0.0143 | 0.0163 | 0.0554 | 0.0391 | 0.0233 | 0.0132 | 0.0102 | 0.008 | 0.0417 | 0.028 |
Nucleus | 0.8144 | 0.9742 | 0.9637 | 0.9235 | 0.7932 | 0.8754 | 0.8829 | 0.9716 | 0.9268 | 0.8843 | 0.8818 | 0.9361 | 0.9049 | 0.9383 | 0.9473 | 0.9118 | 0.811 | 0.9434 |
Peroxisomes | 0.0043 | 0 | 0.0003 | 0.0325 | 0.0168 | 0.0078 | 0.0042 | 0 | 0.0043 | 0.0009 | 0.0033 | 0.0004 | 0.0019 | 0 | 0.0003 | 0 | 0.0011 | 0.0001 |
Punctate Nuclear | 0.0139 | 0.0051 | 0.0013 | 0.0086 | 0.035 | 0.0091 | 0.0078 | 0.0052 | 0.0031 | 0.0153 | 0.0047 | 0.0079 | 0.0404 | 0.0473 | 0.0143 | 0.0797 | 0.0828 | 0.0228 |
Vacuole | 0.0022 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0009 | 0.0052 | 0.0001 | 0.0012 | 0.0012 | 0.0009 | 0.0006 | 0.0015 | 0 | 0.0006 | 0 | 0.0029 | 0 |
Vacuole Periphery | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0026 | 0 | 0.0004 | 0.0007 | 0.0015 | 0.0002 | 0.0005 | 0 | 0.0002 | 0 | 0.0008 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.0409 | 6.9799 | 7.0876 | 15.1558 | 9.0579 | 8.2145 | 11.0504 | 14.9166 | 13.4789 | 12.8249 |
Translational Efficiency | 1.1373 | 1.3015 | 1.236 | 0.7256 | 1.0185 | 1.6129 | 0.84 | 0.7954 | 1.045 | 0.8515 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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