Standard name
Human Ortholog
Description Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.18 0.15
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.19 0.18
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.12 0.08
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 – 0.96 0.89 0.96 0.93 0.91 0.86 0.81 0.81 0.72 0.75 0.94 0.93 0.88 0.87 0.82 0.81 0.77 0.78 0.86 0.41 0.36 0.48
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 – 0.05 0.11 0.06 0.18 0.21 0.27 0.38 0.4 0.52 0.47 0 0 0.06 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.11 0.19 0.22 0.21 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 14 35 48
Bud 0 – 5 3 3 3 5 7 15 14 9 13 2 0 1 0 0 0 0 0 0 10 36 56
Bud Neck 0 – 0 0 0 0 1 3 0 1 2 1 0 0 0 0 0 0 0 0 0 8 23 27
Bud Site 0 – 0 0 1 0 2 1 1 2 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 2 4 3 3 1 2 2 5 1 2 0 1 0 3 0 0 0 0 3 3
Cytoplasm 47 – 144 300 626 499 825 886 586 670 334 567 136 268 290 286 168 216 56 22 142 30 69 152
Endoplasmic Reticulum 0 – 1 2 1 2 0 3 0 1 1 0 0 1 2 16 10 8 2 0 1 0 0 2
Endosome 0 – 0 0 3 0 1 1 0 0 0 0 1 1 1 4 4 4 2 0 1 3 5 5
Golgi 0 – 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 2 3 0 0 0 0 0
Mitochondria 7 – 8 37 41 98 193 274 277 333 241 355 1 0 21 10 1 5 2 0 4 1 5 5
Nucleus 0 – 0 6 2 1 3 3 8 3 2 4 2 3 4 11 9 6 0 3 0 0 1 0
Nuclear Periphery 0 – 0 0 0 0 1 0 1 2 6 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 1 – 0 0 0 2 0 1 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 1 0 1 0 0 1 2 2 2 1 0 0 0 0 0 1 2 0 1 1 2 4
Vac/Vac Membrane 1 – 2 14 14 10 11 21 13 16 3 18 11 20 37 61 45 55 0 1 5 1 6 8
Unique Cell Count 54 150 336 655 535 908 1027 722 825 467 760 145 289 330 329 205 267 74 29 165 74 193 320
Labelled Cell Count 56 161 363 694 620 1045 1204 904 1048 603 965 154 296 356 391 238 300 74 29 165 74 193 320


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 – 4.0 4.0 4.0 3.7 3.3 3.5 3.0 3.0 3.0 2.8 5.8 5.8 4.9 6.0 6.6 7.3 5.7 5.5 6.1
Std Deviation (1e-4) 0.7 – 1.2 0.5 0.7 0.9 0.7 0.8 0.8 0.7 0.8 0.6 1.1 1.1 0.9 1.4 1.5 1.7 1.6 1.3 1.2
Intensity Change (Log2) – – – -0.01 0.01 -0.13 -0.27 -0.2 -0.42 -0.42 -0.4 -0.52 0.53 0.53 0.3 0.6 0.72 0.88 0.52 0.46 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.4 -0.2 -1.2 -2.1 -3.4 -4.5 -4.5 -6.2 -5.8 -0.9 -1.4 -2.8 -3.0 -4.0 -4.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.4 -1.2 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 2.6 2.5 3.7 5.2 5.7 5.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7362 2.1789 1.9313 1.3635 1.5263 1.9685 2.9354 3.2269 3.0316 2.8847 2.776 2.848 0.4322 1.0616 0.9123 1.2357 0.4961 0.7833
Actin 0.0355 0.002 0.0128 0.0238 0.0258 0.0116 0.048 0.002 0.0403 0.0007 0.0105 0.0025 0.019 0.0013 0.0147 0.0042 0.0074 0.0019
Bud 0.0009 0.0008 0.0017 0.0014 0.0007 0.0007 0.0052 0.0043 0.0018 0.0002 0.0007 0.0017 0.0009 0.0005 0.0001 0.0045 0.0059 0.0034
Bud Neck 0.0095 0.001 0.0091 0.002 0.0017 0.0093 0.0093 0.0005 0.002 0.0006 0.0011 0.0039 0.0083 0.0006 0.0002 0.0018 0.0004 0.0023
Bud Periphery 0.0015 0.0006 0.0018 0.002 0.0023 0.0009 0.0069 0.0028 0.0018 0.0001 0.0007 0.0022 0.0014 0.0003 0.0001 0.0049 0.0097 0.0067
Bud Site 0.0082 0.0097 0.0155 0.0016 0.0008 0.0005 0.0145 0.0055 0.0048 0.0002 0.0004 0.0002 0.0086 0.036 0.0003 0.0472 0.0047 0.0007
Cell Periphery 0.0006 0.0003 0.0004 0.0003 0.0003 0.0001 0.0007 0.0001 0.0002 0 0.0001 0 0.0006 0.0009 0.0001 0.0007 0.0002 0.0003
Cytoplasm 0.7254 0.7795 0.7468 0.7992 0.7835 0.8131 0.5552 0.8081 0.7692 0.79 0.651 0.7997 0.6123 0.6863 0.613 0.6273 0.6177 0.6806
Cytoplasmic Foci 0.0144 0.0105 0.0144 0.0095 0.0104 0.0099 0.0226 0.0092 0.0106 0.0074 0.0176 0.0082 0.0105 0.0029 0.0089 0.0143 0.0091 0.0029
Eisosomes 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0008 0 0.0008 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0117 0.0077 0.0074 0.0125 0.0098 0.0079 0.0054 0.0032 0.002 0.0065 0.0089 0.0035 0.0031 0.0032 0.003 0.0083 0.0028 0.0027
Endosome 0.0197 0.0045 0.008 0.0049 0.0146 0.0065 0.0128 0.002 0.0117 0.021 0.0196 0.0072 0.0122 0.0008 0.0022 0.0025 0.0046 0.0022
Golgi 0.0084 0.0004 0.0022 0.0031 0.0037 0.0008 0.0059 0.0006 0.0048 0.002 0.0124 0.0022 0.0032 0.0001 0.0017 0.0028 0.0033 0.0002
Lipid Particles 0.0038 0.0009 0.0042 0.0034 0.0046 0.0008 0.0239 0.0001 0.0006 0.0053 0.0055 0.0021 0.0025 0.0002 0.0043 0.0041 0.026 0.0001
Mitochondria 0.0057 0.0003 0.0072 0.0104 0.0105 0.0009 0.0157 0.0011 0.0055 0.0007 0.0215 0.0151 0.0012 0.0001 0.0007 0.0025 0.0097 0.0003
None 0.084 0.145 0.1305 0.083 0.0518 0.0616 0.1859 0.1153 0.1323 0.1419 0.2112 0.0409 0.2886 0.262 0.3389 0.213 0.2763 0.2854
Nuclear Periphery 0.0107 0.0025 0.0055 0.0038 0.0089 0.0068 0.0234 0.0022 0.0009 0.0094 0.0039 0.0028 0.0023 0.0009 0.0007 0.0009 0.0017 0.001
Nucleolus 0.0008 0.0004 0.0017 0.0002 0.0006 0.0006 0.0006 0.0002 0.0001 0.0002 0.0002 0.0001 0.0021 0.0001 0.0001 0.0014 0.0004 0.0001
Nucleus 0.0447 0.0278 0.0147 0.0304 0.0515 0.0565 0.0149 0.0359 0.0054 0.0076 0.0142 0.102 0.0124 0.0022 0.0026 0.0024 0.0043 0.0073
Peroxisomes 0.0032 0.0004 0.0071 0.0042 0.0014 0.0006 0.0316 0.0025 0.0029 0.0004 0.0151 0.0006 0.0018 0 0.007 0.0518 0.0126 0.0001
Punctate Nuclear 0.0066 0.003 0.005 0.0021 0.0055 0.0079 0.0128 0.0035 0.0012 0.0032 0.0037 0.0031 0.003 0.0007 0.0007 0.0007 0.0014 0.0006
Vacuole 0.0035 0.0025 0.0034 0.0016 0.0068 0.0026 0.0021 0.0007 0.0009 0.0019 0.0009 0.0015 0.0052 0.0008 0.0006 0.0042 0.0012 0.0011
Vacuole Periphery 0.001 0.0002 0.0006 0.0003 0.0046 0.0004 0.0019 0.0001 0.0003 0.0008 0.0007 0.0005 0.0005 0.0001 0.0001 0.0002 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.4236 10.7448 13.5543 18.9335 13.7659 9.21 19.5554 18.2091 15.2463 20.9174
Translational Efficiency 0.6005 0.8947 0.9019 0.6262 0.7544 1.2822 0.5924 0.6538 0.8665 0.6744

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1594 1021 181 399 1351 1761 411 102 2945 2782 592 501

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.48 779.38 962.42 938.02 629.10 773.67 1020.31 996.91 646.63 775.77 1002.61 950.01
Standard Deviation 85.21 106.45 122.80 109.32 123.63 98.33 108.22 122.83 105.84 101.42 115.98 114.68
Intensity Change Log 2 — 0.236629 0.540969 0.503921 — 0.298429 0.697646 0.664174 — 0.267085 0.619469 0.584262

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000239 0.000585 0.001160 0.000306 0.000191 0.000427 0.000409 0.000982 0.000217 0.000485 0.000638 0.000444
Bud Neck 0.002188 0.008067 0.003149 0.003536 0.005726 0.021817 0.003922 0.003969 0.003811 0.016771 0.003686 0.003624
Bud Site 0.003353 0.008953 0.004899 0.007701 0.002350 0.014440 0.008885 0.006940 0.002893 0.012426 0.007667 0.007546
Cell Periphery 0.000123 0.000161 0.000315 0.000210 0.000164 0.000133 0.000195 0.000223 0.000142 0.000143 0.000231 0.000213
Cytoplasm 0.638568* 0.536855* 0.376339* 0.508311* 0.562636* 0.514819* 0.342807* 0.365617* 0.603735* 0.522907* 0.353059* 0.479259*
Cytoplasmic Foci 0.067790 0.146338 0.010667 0.033139 0.112765 0.162325 0.011502 0.008266 0.088422 0.156458 0.011247 0.028075
Eisosomes 0.000046 0.000079 0.000043 0.000075 0.000090 0.000105 0.000053 0.000059 0.000066 0.000096 0.000050 0.000072
Endoplasmic Reticulum 0.000494 0.001807 0.008274 0.001705 0.001192 0.000795 0.005468 0.008522 0.000814 0.001166 0.006326 0.003093
Endosome 0.001960 0.009878 0.002136 0.017604 0.005778 0.012351 0.003280 0.004311 0.003712 0.011444 0.002930 0.014898
Golgi 0.001342 0.001231 0.000227 0.005107 0.001179 0.004744 0.000154 0.000165 0.001267 0.003455 0.000177 0.004101
Lipid Particles 0.001879 0.003648 0.001229 0.007214 0.005689 0.004919 0.001664 0.002745 0.003627 0.004453 0.001531 0.006304
Mitochondria 0.001352 0.001072 0.001000 0.009780 0.002115 0.006008 0.000915 0.000637 0.001702 0.004197 0.000941 0.007919
Mitotic Spindle 0.002118 0.001095 0.001299 0.021001 0.000632 0.014776 0.017489 0.007334 0.001436 0.009755 0.012539 0.018219
None 0.019497 0.003849 0.004294 0.010789 0.014522 0.002070 0.014411 0.039870 0.017215 0.002723 0.011318 0.016710
Nuclear Periphery 0.000186 0.000444 0.001044 0.001052 0.000791 0.000351 0.002173 0.002376 0.000463 0.000385 0.001828 0.001322
Nuclear Periphery Foci 0.000825 0.001735 0.005550 0.004774 0.001016 0.000530 0.002395 0.007066 0.000913 0.000972 0.003360 0.005241
Nucleolus 0.001343 0.000968 0.000309 0.000314 0.002529 0.001111 0.000308 0.001014 0.001887 0.001059 0.000308 0.000456
Nucleus 0.239992* 0.193770* 0.486334* 0.192573* 0.244278* 0.161547* 0.446470* 0.330326* 0.241958* 0.173373* 0.458658* 0.220619*
Peroxisomes 0.000892 0.005389 0.000170 0.001474 0.002541 0.009042 0.000200 0.000357 0.001648 0.007701 0.000191 0.001247
Vacuole 0.015533 0.073026 0.090768 0.172045 0.033134 0.066570 0.136717 0.208976* 0.023607 0.068939 0.122669 0.179564
Vacuole Periphery 0.000280 0.001050 0.000794 0.001290 0.000684 0.001118 0.000583 0.000246 0.000465 0.001093 0.000648 0.001077

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.59 -4.27 -1.21 0.99 4.10 -2.05 -3.79 -4.83 -3.76 -2.90 -2.39 -5.36 -3.26 -0.46 3.00
Bud Neck -6.19 -3.07 -4.32 2.60 -2.14 -9.61 0.61 -0.11 8.98 -0.59 -11.81 -2.15 -2.53 9.76 -0.96
Bud Site -4.67 -2.41 -3.53 0.19 -2.13 -8.41 -3.22 -4.57 2.91 -1.00 -9.07 -3.38 -5.01 2.56 -1.60
Cell Periphery -2.89 -3.98 -1.21 1.53 3.63 0.72 -0.02 -0.24 -2.74 -0.56 -0.04 -2.64 -0.18 -0.31 3.50
Cytoplasm 10.64 15.73 19.99 12.00 -0.34 4.94 22.35 12.29 10.35 -0.21 12.09 29.55 21.71 15.06 -2.40
Cytoplasmic Foci -13.05 19.81 15.80 22.33 -2.78 -8.19 25.41 25.43 34.24 -0.22 -16.27 32.31 26.01 37.36 -3.84
Eisosomes -7.79 -1.90 -6.91 -0.69 -3.88 -0.76 1.05 0.84 3.57 -0.41 -3.25 -0.04 -1.73 2.35 -2.95
Endoplasmic Reticulum -7.23 -8.24 -10.15 -5.01 5.53 1.53 -10.80 -5.70 -6.16 -0.22 -2.57 -14.04 -10.27 -9.32 6.23
Endosome -10.03 -1.94 -2.66 1.77 -2.18 -7.34 5.46 3.25 9.08 -0.41 -12.70 2.61 -1.54 3.72 -2.18
Golgi 0.26 2.05 0.07 -0.11 -1.42 -4.12 2.79 2.67 6.04 -1.70 -3.57 3.25 0.31 3.01 -1.51
Lipid Particles -4.26 0.62 -1.01 0.46 -1.17 1.11 6.57 4.27 2.95 -2.61 -1.63 5.78 0.57 1.31 -1.26
Mitochondria 0.66 1.09 -0.66 -0.80 -0.94 -3.22 1.92 2.30 5.58 2.26 -3.40 2.30 -0.35 1.06 -0.91
Mitotic Spindle 0.69 0.97 -4.48 -4.64 -4.69 -6.52 -2.61 -2.04 -0.67 -1.21 -5.77 -2.25 -5.01 -3.34 -3.79
None 9.09 9.60 5.23 -3.77 -4.27 7.88 3.23 0.24 -2.62 -1.39 12.47 6.74 4.27 -5.31 -2.17
Nuclear Periphery -7.54 -10.44 -13.50 -11.57 -5.14 1.84 -7.76 -4.44 -6.15 0.75 0.57 -11.70 -10.80 -13.45 0.78
Nuclear Periphery Foci -4.78 -6.26 -3.18 -2.04 2.46 3.79 -3.73 -3.31 -3.69 -1.86 -0.64 -6.98 -4.09 -3.99 0.08
Nucleolus 1.07 3.15 1.28 0.52 -2.19 4.30 6.49 5.65 3.23 -0.83 3.49 6.46 3.68 1.25 -2.09
Nucleus 5.48 -11.73 -0.40 -3.93 10.40 11.03 -13.88 -2.52 -5.93 5.69 12.60 -18.24 -1.41 -7.66 13.61
Peroxisomes -5.03 3.54 1.08 5.41 -1.69 -6.06 4.04 3.82 9.85 -2.00 -8.46 5.19 3.11 10.54 -1.85
Vacuole -16.51 -8.88 -20.61 -15.98 -9.56 -11.08 -14.54 -10.15 -9.02 -3.85 -20.99 -17.24 -22.62 -18.92 -8.35
Vacuole Periphery -2.40 -0.68 -1.00 1.67 0.24 -2.00 3.49 5.43 4.93 0.99 -3.54 0.88 0.54 3.30 -0.28
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation
Localization
Cell Percentages cytoplasm (77%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ste5

Ste5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available