Standard name | |
---|---|
Human Ortholog | |
Description | Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 14 | 35 | 48 |
Bud | 0 | – | 5 | 3 | 3 | 3 | 5 | 7 | 15 | 14 | 9 | 13 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 36 | 56 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 23 | 27 |
Bud Site | 0 | – | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 0 | 2 | 4 | 3 | 3 | 1 | 2 | 2 | 5 | 1 | 2 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 3 | 3 |
Cytoplasm | 47 | – | 144 | 300 | 626 | 499 | 825 | 886 | 586 | 670 | 334 | 567 | 136 | 268 | 290 | 286 | 168 | 216 | 56 | 22 | 142 | 30 | 69 | 152 |
Endoplasmic Reticulum | 0 | – | 1 | 2 | 1 | 2 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 16 | 10 | 8 | 2 | 0 | 1 | 0 | 0 | 2 |
Endosome | 0 | – | 0 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 4 | 4 | 2 | 0 | 1 | 3 | 5 | 5 |
Golgi | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | – | 8 | 37 | 41 | 98 | 193 | 274 | 277 | 333 | 241 | 355 | 1 | 0 | 21 | 10 | 1 | 5 | 2 | 0 | 4 | 1 | 5 | 5 |
Nucleus | 0 | – | 0 | 6 | 2 | 1 | 3 | 3 | 8 | 3 | 2 | 4 | 2 | 3 | 4 | 11 | 9 | 6 | 0 | 3 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 2 | 4 |
Vac/Vac Membrane | 1 | – | 2 | 14 | 14 | 10 | 11 | 21 | 13 | 16 | 3 | 18 | 11 | 20 | 37 | 61 | 45 | 55 | 0 | 1 | 5 | 1 | 6 | 8 |
Unique Cell Count | 54 | 150 | 336 | 655 | 535 | 908 | 1027 | 722 | 825 | 467 | 760 | 145 | 289 | 330 | 329 | 205 | 267 | 74 | 29 | 165 | 74 | 193 | 320 | |
Labelled Cell Count | 56 | 161 | 363 | 694 | 620 | 1045 | 1204 | 904 | 1048 | 603 | 965 | 154 | 296 | 356 | 391 | 238 | 300 | 74 | 29 | 165 | 74 | 193 | 320 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | – | 4.0 | 4.0 | 4.0 | 3.7 | 3.3 | 3.5 | 3.0 | 3.0 | 3.0 | 2.8 | 5.8 | 5.8 | 4.9 | 6.0 | 6.6 | 7.3 | 5.7 | 5.5 | 6.1 |
Std Deviation (1e-4) | 0.7 | – | 1.2 | 0.5 | 0.7 | 0.9 | 0.7 | 0.8 | 0.8 | 0.7 | 0.8 | 0.6 | 1.1 | 1.1 | 0.9 | 1.4 | 1.5 | 1.7 | 1.6 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.01 | -0.13 | -0.27 | -0.2 | -0.42 | -0.42 | -0.4 | -0.52 | 0.53 | 0.53 | 0.3 | 0.6 | 0.72 | 0.88 | 0.52 | 0.46 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.4 | -0.2 | -1.2 | -2.1 | -3.4 | -4.5 | -4.5 | -6.2 | -5.8 | -0.9 | -1.4 | -2.8 | -3.0 | -4.0 | -4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | -1.2 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.5 | 3.7 | 5.2 | 5.7 | 5.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7362 | 2.1789 | 1.9313 | 1.3635 | 1.5263 | 1.9685 | 2.9354 | 3.2269 | 3.0316 | 2.8847 | 2.776 | 2.848 | 0.4322 | 1.0616 | 0.9123 | 1.2357 | 0.4961 | 0.7833 |
Actin | 0.0355 | 0.002 | 0.0128 | 0.0238 | 0.0258 | 0.0116 | 0.048 | 0.002 | 0.0403 | 0.0007 | 0.0105 | 0.0025 | 0.019 | 0.0013 | 0.0147 | 0.0042 | 0.0074 | 0.0019 |
Bud | 0.0009 | 0.0008 | 0.0017 | 0.0014 | 0.0007 | 0.0007 | 0.0052 | 0.0043 | 0.0018 | 0.0002 | 0.0007 | 0.0017 | 0.0009 | 0.0005 | 0.0001 | 0.0045 | 0.0059 | 0.0034 |
Bud Neck | 0.0095 | 0.001 | 0.0091 | 0.002 | 0.0017 | 0.0093 | 0.0093 | 0.0005 | 0.002 | 0.0006 | 0.0011 | 0.0039 | 0.0083 | 0.0006 | 0.0002 | 0.0018 | 0.0004 | 0.0023 |
Bud Periphery | 0.0015 | 0.0006 | 0.0018 | 0.002 | 0.0023 | 0.0009 | 0.0069 | 0.0028 | 0.0018 | 0.0001 | 0.0007 | 0.0022 | 0.0014 | 0.0003 | 0.0001 | 0.0049 | 0.0097 | 0.0067 |
Bud Site | 0.0082 | 0.0097 | 0.0155 | 0.0016 | 0.0008 | 0.0005 | 0.0145 | 0.0055 | 0.0048 | 0.0002 | 0.0004 | 0.0002 | 0.0086 | 0.036 | 0.0003 | 0.0472 | 0.0047 | 0.0007 |
Cell Periphery | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0006 | 0.0009 | 0.0001 | 0.0007 | 0.0002 | 0.0003 |
Cytoplasm | 0.7254 | 0.7795 | 0.7468 | 0.7992 | 0.7835 | 0.8131 | 0.5552 | 0.8081 | 0.7692 | 0.79 | 0.651 | 0.7997 | 0.6123 | 0.6863 | 0.613 | 0.6273 | 0.6177 | 0.6806 |
Cytoplasmic Foci | 0.0144 | 0.0105 | 0.0144 | 0.0095 | 0.0104 | 0.0099 | 0.0226 | 0.0092 | 0.0106 | 0.0074 | 0.0176 | 0.0082 | 0.0105 | 0.0029 | 0.0089 | 0.0143 | 0.0091 | 0.0029 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0117 | 0.0077 | 0.0074 | 0.0125 | 0.0098 | 0.0079 | 0.0054 | 0.0032 | 0.002 | 0.0065 | 0.0089 | 0.0035 | 0.0031 | 0.0032 | 0.003 | 0.0083 | 0.0028 | 0.0027 |
Endosome | 0.0197 | 0.0045 | 0.008 | 0.0049 | 0.0146 | 0.0065 | 0.0128 | 0.002 | 0.0117 | 0.021 | 0.0196 | 0.0072 | 0.0122 | 0.0008 | 0.0022 | 0.0025 | 0.0046 | 0.0022 |
Golgi | 0.0084 | 0.0004 | 0.0022 | 0.0031 | 0.0037 | 0.0008 | 0.0059 | 0.0006 | 0.0048 | 0.002 | 0.0124 | 0.0022 | 0.0032 | 0.0001 | 0.0017 | 0.0028 | 0.0033 | 0.0002 |
Lipid Particles | 0.0038 | 0.0009 | 0.0042 | 0.0034 | 0.0046 | 0.0008 | 0.0239 | 0.0001 | 0.0006 | 0.0053 | 0.0055 | 0.0021 | 0.0025 | 0.0002 | 0.0043 | 0.0041 | 0.026 | 0.0001 |
Mitochondria | 0.0057 | 0.0003 | 0.0072 | 0.0104 | 0.0105 | 0.0009 | 0.0157 | 0.0011 | 0.0055 | 0.0007 | 0.0215 | 0.0151 | 0.0012 | 0.0001 | 0.0007 | 0.0025 | 0.0097 | 0.0003 |
None | 0.084 | 0.145 | 0.1305 | 0.083 | 0.0518 | 0.0616 | 0.1859 | 0.1153 | 0.1323 | 0.1419 | 0.2112 | 0.0409 | 0.2886 | 0.262 | 0.3389 | 0.213 | 0.2763 | 0.2854 |
Nuclear Periphery | 0.0107 | 0.0025 | 0.0055 | 0.0038 | 0.0089 | 0.0068 | 0.0234 | 0.0022 | 0.0009 | 0.0094 | 0.0039 | 0.0028 | 0.0023 | 0.0009 | 0.0007 | 0.0009 | 0.0017 | 0.001 |
Nucleolus | 0.0008 | 0.0004 | 0.0017 | 0.0002 | 0.0006 | 0.0006 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0021 | 0.0001 | 0.0001 | 0.0014 | 0.0004 | 0.0001 |
Nucleus | 0.0447 | 0.0278 | 0.0147 | 0.0304 | 0.0515 | 0.0565 | 0.0149 | 0.0359 | 0.0054 | 0.0076 | 0.0142 | 0.102 | 0.0124 | 0.0022 | 0.0026 | 0.0024 | 0.0043 | 0.0073 |
Peroxisomes | 0.0032 | 0.0004 | 0.0071 | 0.0042 | 0.0014 | 0.0006 | 0.0316 | 0.0025 | 0.0029 | 0.0004 | 0.0151 | 0.0006 | 0.0018 | 0 | 0.007 | 0.0518 | 0.0126 | 0.0001 |
Punctate Nuclear | 0.0066 | 0.003 | 0.005 | 0.0021 | 0.0055 | 0.0079 | 0.0128 | 0.0035 | 0.0012 | 0.0032 | 0.0037 | 0.0031 | 0.003 | 0.0007 | 0.0007 | 0.0007 | 0.0014 | 0.0006 |
Vacuole | 0.0035 | 0.0025 | 0.0034 | 0.0016 | 0.0068 | 0.0026 | 0.0021 | 0.0007 | 0.0009 | 0.0019 | 0.0009 | 0.0015 | 0.0052 | 0.0008 | 0.0006 | 0.0042 | 0.0012 | 0.0011 |
Vacuole Periphery | 0.001 | 0.0002 | 0.0006 | 0.0003 | 0.0046 | 0.0004 | 0.0019 | 0.0001 | 0.0003 | 0.0008 | 0.0007 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.4236 | 10.7448 | 13.5543 | 18.9335 | 13.7659 | 9.21 | 19.5554 | 18.2091 | 15.2463 | 20.9174 |
Translational Efficiency | 0.6005 | 0.8947 | 0.9019 | 0.6262 | 0.7544 | 1.2822 | 0.5924 | 0.6538 | 0.8665 | 0.6744 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1594 | 1021 | 181 | 399 | 1351 | 1761 | 411 | 102 | 2945 | 2782 | 592 | 501 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 661.48 | 779.38 | 962.42 | 938.02 | 629.10 | 773.67 | 1020.31 | 996.91 | 646.63 | 775.77 | 1002.61 | 950.01 |
Standard Deviation | 85.21 | 106.45 | 122.80 | 109.32 | 123.63 | 98.33 | 108.22 | 122.83 | 105.84 | 101.42 | 115.98 | 114.68 |
Intensity Change Log 2 | — | 0.236629 | 0.540969 | 0.503921 | — | 0.298429 | 0.697646 | 0.664174 | — | 0.267085 | 0.619469 | 0.584262 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000239 | 0.000585 | 0.001160 | 0.000306 | 0.000191 | 0.000427 | 0.000409 | 0.000982 | 0.000217 | 0.000485 | 0.000638 | 0.000444 |
Bud Neck | 0.002188 | 0.008067 | 0.003149 | 0.003536 | 0.005726 | 0.021817 | 0.003922 | 0.003969 | 0.003811 | 0.016771 | 0.003686 | 0.003624 |
Bud Site | 0.003353 | 0.008953 | 0.004899 | 0.007701 | 0.002350 | 0.014440 | 0.008885 | 0.006940 | 0.002893 | 0.012426 | 0.007667 | 0.007546 |
Cell Periphery | 0.000123 | 0.000161 | 0.000315 | 0.000210 | 0.000164 | 0.000133 | 0.000195 | 0.000223 | 0.000142 | 0.000143 | 0.000231 | 0.000213 |
Cytoplasm | 0.638568* | 0.536855* | 0.376339* | 0.508311* | 0.562636* | 0.514819* | 0.342807* | 0.365617* | 0.603735* | 0.522907* | 0.353059* | 0.479259* |
Cytoplasmic Foci | 0.067790 | 0.146338 | 0.010667 | 0.033139 | 0.112765 | 0.162325 | 0.011502 | 0.008266 | 0.088422 | 0.156458 | 0.011247 | 0.028075 |
Eisosomes | 0.000046 | 0.000079 | 0.000043 | 0.000075 | 0.000090 | 0.000105 | 0.000053 | 0.000059 | 0.000066 | 0.000096 | 0.000050 | 0.000072 |
Endoplasmic Reticulum | 0.000494 | 0.001807 | 0.008274 | 0.001705 | 0.001192 | 0.000795 | 0.005468 | 0.008522 | 0.000814 | 0.001166 | 0.006326 | 0.003093 |
Endosome | 0.001960 | 0.009878 | 0.002136 | 0.017604 | 0.005778 | 0.012351 | 0.003280 | 0.004311 | 0.003712 | 0.011444 | 0.002930 | 0.014898 |
Golgi | 0.001342 | 0.001231 | 0.000227 | 0.005107 | 0.001179 | 0.004744 | 0.000154 | 0.000165 | 0.001267 | 0.003455 | 0.000177 | 0.004101 |
Lipid Particles | 0.001879 | 0.003648 | 0.001229 | 0.007214 | 0.005689 | 0.004919 | 0.001664 | 0.002745 | 0.003627 | 0.004453 | 0.001531 | 0.006304 |
Mitochondria | 0.001352 | 0.001072 | 0.001000 | 0.009780 | 0.002115 | 0.006008 | 0.000915 | 0.000637 | 0.001702 | 0.004197 | 0.000941 | 0.007919 |
Mitotic Spindle | 0.002118 | 0.001095 | 0.001299 | 0.021001 | 0.000632 | 0.014776 | 0.017489 | 0.007334 | 0.001436 | 0.009755 | 0.012539 | 0.018219 |
None | 0.019497 | 0.003849 | 0.004294 | 0.010789 | 0.014522 | 0.002070 | 0.014411 | 0.039870 | 0.017215 | 0.002723 | 0.011318 | 0.016710 |
Nuclear Periphery | 0.000186 | 0.000444 | 0.001044 | 0.001052 | 0.000791 | 0.000351 | 0.002173 | 0.002376 | 0.000463 | 0.000385 | 0.001828 | 0.001322 |
Nuclear Periphery Foci | 0.000825 | 0.001735 | 0.005550 | 0.004774 | 0.001016 | 0.000530 | 0.002395 | 0.007066 | 0.000913 | 0.000972 | 0.003360 | 0.005241 |
Nucleolus | 0.001343 | 0.000968 | 0.000309 | 0.000314 | 0.002529 | 0.001111 | 0.000308 | 0.001014 | 0.001887 | 0.001059 | 0.000308 | 0.000456 |
Nucleus | 0.239992* | 0.193770* | 0.486334* | 0.192573* | 0.244278* | 0.161547* | 0.446470* | 0.330326* | 0.241958* | 0.173373* | 0.458658* | 0.220619* |
Peroxisomes | 0.000892 | 0.005389 | 0.000170 | 0.001474 | 0.002541 | 0.009042 | 0.000200 | 0.000357 | 0.001648 | 0.007701 | 0.000191 | 0.001247 |
Vacuole | 0.015533 | 0.073026 | 0.090768 | 0.172045 | 0.033134 | 0.066570 | 0.136717 | 0.208976* | 0.023607 | 0.068939 | 0.122669 | 0.179564 |
Vacuole Periphery | 0.000280 | 0.001050 | 0.000794 | 0.001290 | 0.000684 | 0.001118 | 0.000583 | 0.000246 | 0.000465 | 0.001093 | 0.000648 | 0.001077 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.59 | -4.27 | -1.21 | 0.99 | 4.10 | -2.05 | -3.79 | -4.83 | -3.76 | -2.90 | -2.39 | -5.36 | -3.26 | -0.46 | 3.00 |
Bud Neck | -6.19 | -3.07 | -4.32 | 2.60 | -2.14 | -9.61 | 0.61 | -0.11 | 8.98 | -0.59 | -11.81 | -2.15 | -2.53 | 9.76 | -0.96 |
Bud Site | -4.67 | -2.41 | -3.53 | 0.19 | -2.13 | -8.41 | -3.22 | -4.57 | 2.91 | -1.00 | -9.07 | -3.38 | -5.01 | 2.56 | -1.60 |
Cell Periphery | -2.89 | -3.98 | -1.21 | 1.53 | 3.63 | 0.72 | -0.02 | -0.24 | -2.74 | -0.56 | -0.04 | -2.64 | -0.18 | -0.31 | 3.50 |
Cytoplasm | 10.64 | 15.73 | 19.99 | 12.00 | -0.34 | 4.94 | 22.35 | 12.29 | 10.35 | -0.21 | 12.09 | 29.55 | 21.71 | 15.06 | -2.40 |
Cytoplasmic Foci | -13.05 | 19.81 | 15.80 | 22.33 | -2.78 | -8.19 | 25.41 | 25.43 | 34.24 | -0.22 | -16.27 | 32.31 | 26.01 | 37.36 | -3.84 |
Eisosomes | -7.79 | -1.90 | -6.91 | -0.69 | -3.88 | -0.76 | 1.05 | 0.84 | 3.57 | -0.41 | -3.25 | -0.04 | -1.73 | 2.35 | -2.95 |
Endoplasmic Reticulum | -7.23 | -8.24 | -10.15 | -5.01 | 5.53 | 1.53 | -10.80 | -5.70 | -6.16 | -0.22 | -2.57 | -14.04 | -10.27 | -9.32 | 6.23 |
Endosome | -10.03 | -1.94 | -2.66 | 1.77 | -2.18 | -7.34 | 5.46 | 3.25 | 9.08 | -0.41 | -12.70 | 2.61 | -1.54 | 3.72 | -2.18 |
Golgi | 0.26 | 2.05 | 0.07 | -0.11 | -1.42 | -4.12 | 2.79 | 2.67 | 6.04 | -1.70 | -3.57 | 3.25 | 0.31 | 3.01 | -1.51 |
Lipid Particles | -4.26 | 0.62 | -1.01 | 0.46 | -1.17 | 1.11 | 6.57 | 4.27 | 2.95 | -2.61 | -1.63 | 5.78 | 0.57 | 1.31 | -1.26 |
Mitochondria | 0.66 | 1.09 | -0.66 | -0.80 | -0.94 | -3.22 | 1.92 | 2.30 | 5.58 | 2.26 | -3.40 | 2.30 | -0.35 | 1.06 | -0.91 |
Mitotic Spindle | 0.69 | 0.97 | -4.48 | -4.64 | -4.69 | -6.52 | -2.61 | -2.04 | -0.67 | -1.21 | -5.77 | -2.25 | -5.01 | -3.34 | -3.79 |
None | 9.09 | 9.60 | 5.23 | -3.77 | -4.27 | 7.88 | 3.23 | 0.24 | -2.62 | -1.39 | 12.47 | 6.74 | 4.27 | -5.31 | -2.17 |
Nuclear Periphery | -7.54 | -10.44 | -13.50 | -11.57 | -5.14 | 1.84 | -7.76 | -4.44 | -6.15 | 0.75 | 0.57 | -11.70 | -10.80 | -13.45 | 0.78 |
Nuclear Periphery Foci | -4.78 | -6.26 | -3.18 | -2.04 | 2.46 | 3.79 | -3.73 | -3.31 | -3.69 | -1.86 | -0.64 | -6.98 | -4.09 | -3.99 | 0.08 |
Nucleolus | 1.07 | 3.15 | 1.28 | 0.52 | -2.19 | 4.30 | 6.49 | 5.65 | 3.23 | -0.83 | 3.49 | 6.46 | 3.68 | 1.25 | -2.09 |
Nucleus | 5.48 | -11.73 | -0.40 | -3.93 | 10.40 | 11.03 | -13.88 | -2.52 | -5.93 | 5.69 | 12.60 | -18.24 | -1.41 | -7.66 | 13.61 |
Peroxisomes | -5.03 | 3.54 | 1.08 | 5.41 | -1.69 | -6.06 | 4.04 | 3.82 | 9.85 | -2.00 | -8.46 | 5.19 | 3.11 | 10.54 | -1.85 |
Vacuole | -16.51 | -8.88 | -20.61 | -15.98 | -9.56 | -11.08 | -14.54 | -10.15 | -9.02 | -3.85 | -20.99 | -17.24 | -22.62 | -18.92 | -8.35 |
Vacuole Periphery | -2.40 | -0.68 | -1.00 | 1.67 | 0.24 | -2.00 | 3.49 | 5.43 | 4.93 | 0.99 | -3.54 | 0.88 | 0.54 | 3.30 | -0.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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