ORF
Human Ortholog
Description F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.95 0.95 0.96 0.95 0.93 0.92 0.91 0.84 0.85 0.76 0.81 0.98 0.97 0.95 0.84 0.86 0.85 0.67 0.6 0.54 0.65 0.72 0.68
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0 0 0 0.16 0.12 0.34 0.16 0 0 0 0 0 0 0.11 0.07 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0.05 0 0 0.07 0.07 0 0.06 0 0 0.07 0.18 0.18 0.17 0.1 0.27 0.29 0.22 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 1 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1 1 0 1 3
Bud 1 0 0 1 0 1 1 5 3 4 10 2 1 1 0 0 0 0 0 0 0 0 2 6
Bud Neck 0 1 2 5 11 3 10 20 11 19 5 7 0 0 1 2 3 0 2 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 6 1 1 4 1 3 2 0 0 0 0 1 5 9 7 0 0 0 0 0 0
Cytoplasm 146 281 53 225 330 340 538 658 319 368 334 433 158 377 451 251 341 334 117 182 47 51 129 184
Endoplasmic Reticulum 3 3 0 1 0 0 3 0 0 1 2 1 0 3 0 17 27 32 2 1 0 1 0 1
Endosome 0 0 0 0 0 0 3 4 4 2 0 4 0 4 3 3 3 4 8 3 1 0 1 3
Golgi 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 4 1 0 1 0 0 0 1
Mitochondria 19 5 0 2 0 13 25 24 61 54 149 86 1 0 3 1 6 2 18 21 6 3 5 9
Nucleus 0 0 0 1 0 4 4 6 3 4 4 1 0 5 2 2 4 4 0 2 0 1 0 0
Nuclear Periphery 1 0 0 1 0 1 0 1 0 0 0 2 0 0 0 1 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 1 0 0 1 5 5 11 15 5 12 6 19 0 0 1 0 1 1 1 2 1 0 1 1
Vac/Vac Membrane 3 9 3 4 9 18 24 20 25 31 10 32 3 7 34 53 71 66 17 82 25 17 29 51
Unique Cell Count 161 295 56 235 346 367 584 721 381 434 439 536 161 388 475 298 396 391 176 305 88 79 179 272
Labelled Cell Count 175 300 58 248 356 386 623 757 434 499 522 589 163 397 496 336 471 452 176 305 88 79 179 272


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.2 5.3 4.8 5.2 4.4 4.5 4.8 4.0 4.0 3.8 4.0 5.3 5.2 5.2 5.9 5.7 5.6 5.2 5.4 5.4
Std Deviation (1e-4) 0.7 0.7 0.8 1.0 1.3 1.2 1.3 1.5 1.4 1.1 1.4 1.1 1.4 1.3 1.7 1.3 1.4 1.5 1.9 1.9 1.8
Intensity Change (Log2) – – – -0.12 -0.01 -0.25 -0.23 -0.13 -0.39 -0.39 -0.48 -0.4 0.0 -0.01 -0.03 0.16 0.11 0.09 -0.02 0.05 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.3 2.4 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2429 -0.301 -0.0085 -0.6823 -0.1687 -0.3878 -0.8414 -0.2992 -0.1115 -0.2979 -0.2169 -0.1545 -1.6143 -1.4051 -1.5058 -1.6015 -1.4055 -1.1182
Actin 0.0057 0.0002 0.0144 0.0001 0.0436 0.0155 0.0169 0.0004 0.0152 0.0044 0.0002 0.0004 0.0088 0.0004 0.0368 0.0001 0.0102 0.0006
Bud 0.0015 0.0026 0.0006 0.0018 0.0133 0.0001 0.0005 0.0008 0.0031 0.0004 0.0001 0.0009 0.0196 0.0088 0.0006 0.0005 0.0002 0.0001
Bud Neck 0.0036 0 0.0013 0.0001 0.0018 0.0004 0.0007 0.0002 0.0007 0.0069 0.0001 0.0009 0.0002 0.0002 0.0037 0 0.0006 0.0007
Bud Periphery 0.0033 0.0027 0.0007 0.0024 0.0142 0.0001 0.0015 0.001 0.0017 0.0007 0.0001 0.0023 0.0074 0.0016 0.0007 0.0011 0.0003 0.0002
Bud Site 0.0109 0.0027 0.0062 0.0001 0.0051 0.0001 0.0048 0.0054 0.0101 0.0052 0.0003 0.0007 0.0202 0.0107 0.0124 0.0001 0.0005 0.0001
Cell Periphery 0.0003 0.0001 0.0003 0.0001 0.0004 0 0.0019 0.0002 0.0003 0.0007 0.0001 0.0003 0.0005 0.0004 0.0009 0.0001 0.0001 0.0002
Cytoplasm 0.0227 0.0653 0.0746 0.0454 0.1486 0.1224 0.0188 0.0555 0.0705 0.0496 0.0358 0.1511 0.0588 0.0801 0.0771 0.0528 0.13 0.2185
Cytoplasmic Foci 0.0275 0.0037 0.015 0.0089 0.0226 0.0126 0.0173 0.0163 0.0235 0.0357 0.0769 0.0277 0.0162 0.0167 0.0182 0.0034 0.0373 0.0332
Eisosomes 0.0002 0.0001 0.0003 0.0001 0.0005 0.0001 0.0009 0.0006 0.0007 0.0004 0.0001 0.0001 0.0014 0.0007 0.0005 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0025 0.0011 0.0014 0.0012 0.0031 0.0011 0.0131 0.0011 0.0011 0.0004 0.0016 0.0016 0.0042 0.0018 0.0015 0.0003 0.0046 0.0016
Endosome 0.0362 0.0051 0.0172 0.0018 0.0203 0.0172 0.0451 0.0049 0.0244 0.0127 0.0254 0.0148 0.0447 0.0067 0.0138 0.0004 0.0457 0.0315
Golgi 0.0067 0.0001 0.011 0.0001 0.0095 0.0059 0.0047 0.0034 0.0088 0.0107 0.0111 0.0015 0.0115 0.0003 0.0087 0 0.0344 0.0003
Lipid Particles 0.0399 0.0004 0.0112 0.0006 0.0068 0.0087 0.0145 0.0014 0.0054 0.0144 0.0428 0.0018 0.0207 0.0086 0.0049 0.0001 0.0152 0.0025
Mitochondria 0.0067 0.0003 0.0144 0.0008 0.0064 0.0034 0.0126 0.0062 0.016 0.0271 0.0069 0.0035 0.0064 0.0015 0.0137 0.0003 0.0106 0.0008
None 0.8041 0.9082 0.8107 0.9295 0.6688 0.8065 0.7916 0.8951 0.7837 0.7992 0.7815 0.7494 0.7317 0.8362 0.7762 0.9389 0.6826 0.6533
Nuclear Periphery 0.0033 0.001 0.0009 0.0009 0.0035 0.001 0.0214 0.0003 0.0009 0.0001 0.0018 0.0013 0.0067 0.0009 0.0007 0.0002 0.0032 0.0016
Nucleolus 0.0013 0.0001 0.0015 0.0005 0.0009 0.0001 0.0014 0.001 0.0004 0.0008 0.0005 0.0004 0.0037 0.0052 0.0007 0.0001 0.0005 0.0006
Nucleus 0.0058 0.0005 0.0019 0.0011 0.0078 0.0004 0.0046 0.0009 0.0018 0.0003 0.0005 0.0016 0.0064 0.0026 0.0008 0.0002 0.0025 0.0021
Peroxisomes 0.0089 0.0001 0.007 0.0002 0.0037 0.0012 0.011 0.0015 0.0129 0.027 0.0069 0.0006 0.0087 0.0005 0.0068 0.0001 0.0024 0.0015
Punctate Nuclear 0.0053 0.0002 0.002 0.0016 0.0029 0.0004 0.0028 0.0017 0.0062 0.0005 0.0044 0.0029 0.0027 0.0043 0.0009 0.0002 0.006 0.001
Vacuole 0.0027 0.005 0.0068 0.0024 0.0127 0.0022 0.0093 0.002 0.0111 0.0021 0.0019 0.0321 0.0173 0.0113 0.0194 0.0008 0.0112 0.0451
Vacuole Periphery 0.001 0.0004 0.0007 0.0005 0.0036 0.0005 0.0044 0.0003 0.0014 0.0005 0.0012 0.004 0.0023 0.0008 0.0011 0.0001 0.0014 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.9524 4.7223 3.5042 5.4282 4.2807 7.9195 6.1176 7.2509 7.7801 6.5649
Translational Efficiency 0.8294 0.6113 0.7788 0.5343 0.6276 0.398 0.4641 0.3128 0.3979 0.4165

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
970 525 87 229 1721 1694 167 1859 2691 2219 254 2088

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 591.45 702.04 927.89 836.05 680.69 702.77 914.87 817.23 648.52 702.60 919.33 819.29
Standard Deviation 74.29 101.55 116.21 116.60 80.62 90.60 87.85 93.62 89.34 93.31 98.68 96.58
Intensity Change Log 2 — 0.247297 0.649698 0.499333 — 0.046055 0.426569 0.263744 — 0.143118 0.534611 0.378074

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000042 0.000306 0.001225 0.009139 0.000046 0.000241 0.000438 0.000742 0.000044 0.000256 0.000708 0.001663
Bud Neck 0.022254 0.035336 0.002368 0.008355 0.015801 0.029152 0.002963 0.009819 0.018127 0.030615 0.002759 0.009658
Bud Site 0.004734 0.014772 0.004950 0.019942 0.003340 0.019090 0.002507 0.027693 0.003842 0.018068 0.003344 0.026843
Cell Periphery 0.000555 0.000143 0.000467 0.000179 0.000180 0.000226 0.000197 0.000128 0.000315 0.000206 0.000290 0.000134
Cytoplasm 0.297324* 0.300640* 0.205330* 0.459857* 0.408320* 0.133012* 0.271339* 0.562535* 0.368310* 0.172671* 0.248730* 0.551274*
Cytoplasmic Foci 0.093662 0.215791* 0.023554 0.033912 0.140119 0.068692 0.013013 0.023064 0.123373 0.103495 0.016623 0.024253
Eisosomes 0.000118 0.000142 0.000174 0.000078 0.000092 0.000065 0.000090 0.000038 0.000101 0.000083 0.000119 0.000042
Endoplasmic Reticulum 0.001432 0.000967 0.016109 0.003026 0.000886 0.002309 0.006932 0.001175 0.001083 0.001992 0.010075 0.001378
Endosome 0.020603 0.047698 0.010632 0.014303 0.019259 0.053012 0.005372 0.003374 0.019744 0.051755 0.007173 0.004573
Golgi 0.000482 0.003363 0.000255 0.009655 0.001485 0.002948 0.000114 0.000539 0.001124 0.003047 0.000162 0.001539
Lipid Particles 0.005050 0.008651 0.007887 0.003639 0.006099 0.004181 0.003635 0.001046 0.005721 0.005238 0.005092 0.001330
Mitochondria 0.001921 0.008612 0.000806 0.008893 0.002084 0.008603 0.000490 0.004353 0.002025 0.008605 0.000598 0.004851
Mitotic Spindle 0.000275 0.002497 0.000767 0.026775 0.000390 0.005469 0.019326 0.029265 0.000349 0.004766 0.012969 0.028991
None 0.004169 0.001899 0.004341 0.023110 0.003842 0.001110 0.004838 0.004327 0.003960 0.001296 0.004668 0.006387
Nuclear Periphery 0.000515 0.000812 0.003046 0.001335 0.000399 0.000411 0.002403 0.001338 0.000441 0.000506 0.002623 0.001337
Nuclear Periphery Foci 0.000191 0.000456 0.005910 0.003239 0.000301 0.000181 0.002923 0.000806 0.000262 0.000246 0.003946 0.001073
Nucleolus 0.001403 0.003187 0.001660 0.000809 0.001869 0.001227 0.000746 0.000468 0.001701 0.001691 0.001059 0.000506
Nucleus 0.137027* 0.093767 0.513184* 0.214153* 0.139297* 0.046894 0.517855* 0.208911* 0.138479* 0.057984 0.516255* 0.209486*
Peroxisomes 0.002000 0.016771 0.001081 0.004690 0.002175 0.004873 0.000371 0.000921 0.002112 0.007688 0.000614 0.001335
Vacuole 0.403312* 0.239881* 0.195555 0.154486 0.251938* 0.608050* 0.144187 0.118877 0.306503* 0.520944* 0.161781 0.122782
Vacuole Periphery 0.002929 0.004310 0.000698 0.000423 0.002079 0.010254 0.000261 0.000582 0.002385 0.008848 0.000411 0.000565

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.00 -8.34 -1.52 -1.37 -0.77 -2.16 -8.95 -2.24 -0.99 0.65 -2.94 -11.15 -2.57 -1.67 0.56
Bud Neck -2.86 9.24 3.81 5.39 -2.28 -6.01 8.52 4.80 10.72 -4.75 -6.23 12.42 7.06 12.21 -5.62
Bud Site -4.65 -1.01 -3.27 -0.29 -2.97 -9.48 0.59 -6.48 1.56 -6.61 -10.38 -0.26 -6.83 1.26 -6.72
Cell Periphery 3.49 0.95 3.41 -0.54 5.66 -1.91 -0.35 4.33 8.81 5.39 2.38 0.86 4.86 8.70 7.94
Cytoplasm -0.24 5.56 -0.46 -0.28 -4.71 31.88 10.83 -3.48 -36.65 -12.64 25.93 12.01 -6.92 -31.38 -15.74
Cytoplasmic Foci -11.79 13.50 12.89 19.90 -0.99 13.44 28.76 28.31 14.03 -4.06 4.18 31.13 31.29 22.31 -3.24
Eisosomes -1.41 -4.15 1.61 2.26 5.10 5.19 -1.90 10.91 4.30 6.97 3.10 -3.99 12.74 5.82 10.01
Endoplasmic Reticulum 1.26 -8.30 -5.77 -7.50 6.52 -8.04 -9.78 -11.98 1.26 8.42 -4.91 -11.98 -8.29 -2.56 10.69
Endosome -6.91 5.53 5.24 9.70 1.32 -13.65 11.78 15.19 22.03 3.24 -15.27 12.52 17.91 24.77 5.27
Golgi -3.94 1.89 -1.14 0.36 -1.28 -2.43 3.37 2.92 6.18 -1.67 -4.18 3.65 1.79 5.93 -1.69
Lipid Particles -2.65 -2.94 2.72 4.09 5.03 2.92 3.91 9.52 6.32 5.21 0.86 1.26 11.15 8.05 8.34
Mitochondria -3.61 3.07 -0.28 2.17 -0.85 -5.08 3.24 0.18 4.94 -2.19 -6.19 4.23 0.03 5.61 -2.29
Mitotic Spindle -1.19 -1.61 -3.33 -3.00 -3.26 -3.86 -1.42 -9.59 -7.04 -2.69 -4.04 -1.46 -10.17 -7.87 -4.37
None 2.58 1.00 -1.22 -1.79 -1.53 4.06 -0.62 -1.11 -10.35 0.01 5.01 0.01 -1.64 -7.11 -1.22
Nuclear Periphery -1.08 -9.19 -7.72 -5.44 2.79 -0.22 -11.98 -22.70 -22.21 1.51 -0.77 -15.01 -21.54 -20.15 2.84
Nuclear Periphery Foci -3.32 -8.02 -5.75 -5.42 1.38 2.02 -7.44 -5.81 -6.34 2.90 0.41 -10.57 -7.67 -7.74 4.75
Nucleolus -1.27 0.40 -0.73 0.40 -0.81 1.24 2.34 0.59 -1.03 -3.10 0.10 2.39 0.16 0.06 -2.60
Nucleus 4.86 -11.75 -5.60 -7.98 7.47 19.27 -14.79 -17.92 -35.41 9.98 19.17 -18.70 -19.36 -34.78 12.39
Peroxisomes -6.98 2.42 0.43 6.93 -1.09 -5.27 10.17 6.60 8.33 -3.36 -8.55 7.81 5.30 10.84 -2.35
Vacuole 9.24 5.43 2.60 -4.21 -2.62 -30.35* 1.15 4.35 38.09* 0.79 -20.19 4.01 9.17 30.31 0.81
Vacuole Periphery -1.70 4.07 4.77 8.00 4.24 -10.78 9.44 8.67 13.51 -0.54 -10.35 8.39 8.51 14.75 1.18
ORF
Human Ortholog
Description F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex
Localization
Cell Percentages cytoplasm (30%), vacuole (26%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YDR131C

YDR131C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr131c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available