Standard name
Human Ortholog
Description Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.06 0.07 0.11 0.08 – 0.12 0.2 0.14 0.08 0.08 0 0.1 0.13 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.1 – 0.1 0.09 0.1 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.06 0.08 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Mitochondria 0 0.05 0.05 0.18 0.18 0.28 0.37 0.33 – 0.43 0.44 0.49 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.06 0.09 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.08 0.07 0 0.05 0 0.06 0.05 0
Nuclear Periphery 0.07 0.07 0.3 0.29 0.16 0.15 0.18 0.14 – 0.1 0.07 0.11 0.12 0.14 0.18 0.2 0.23 0.26 0.05 0 0.15 0.09 0.07 0.07
Nucleolus 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.89 0.86 0.8 0.76 0.75 0.64 0.53 0.49 – 0.48 0.38 0.4 0.83 0.84 0.88 0.8 0.75 0.78 0.88 0.87 0.77 0.78 0.78 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 11 – 0 2 2 0 0 0 0 0 0 2 0 1 0 0 0
Bud 0 0 0 0 0 0 1 1 – 2 1 0 0 0 0 0 0 0 6 2 5 1 3 7
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 3 8 7 16 – 11 17 15 1 1 0 3 0 3 0 0 0 0 0 0
Cytoplasm 16 14 17 19 19 41 62 64 – 66 86 71 15 15 12 37 33 12 2 0 1 0 0 0
Endoplasmic Reticulum 0 0 2 4 5 21 29 78 – 54 39 49 2 1 4 10 15 9 1 0 1 1 3 2
Endosome 4 3 6 3 2 2 1 5 – 0 0 0 3 3 0 5 2 3 5 7 4 7 9 15
Golgi 30 5 8 20 27 18 17 20 – 5 5 5 5 4 11 5 10 20 0 0 0 3 1 2
Mitochondria 8 24 19 61 60 161 206 266 – 229 189 245 1 1 2 16 12 10 7 4 6 4 4 6
Nucleus 45 47 15 9 10 14 11 14 – 8 8 10 7 7 9 25 19 15 20 29 7 22 18 17
Nuclear Periphery 52 33 109 100 53 90 101 108 – 55 29 55 23 26 47 72 57 56 42 18 58 31 26 33
Nucleolus 22 9 21 12 4 9 6 14 – 2 1 3 7 7 4 6 8 4 12 4 1 7 10 14
Peroxisomes 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 – 0 0 0 1 1 0 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 696 430 293 259 255 373 296 393 – 256 163 202 156 156 234 287 187 168 744 474 299 288 286 398
Unique Cell Count 779 501 367 340 341 581 557 800 536 433 505 188 186 267 360 248 215 850 548 390 372 369 505
Labelled Cell Count 873 566 490 487 438 739 740 991 689 540 657 221 222 323 466 343 300 850 548 390 372 369 505


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.0 14.6 11.5 11.8 11.4 9.8 9.3 9.6 – 9.1 9.2 8.7 16.7 16.7 18.1 11.7 12.3 14.7 16.1 16.7 18.5
Std Deviation (1e-4) 4.5 4.7 3.6 3.3 3.2 3.1 3.1 2.7 – 2.7 2.6 2.3 5.2 5.2 5.3 3.8 4.4 5.3 4.9 5.1 5.8
Intensity Change (Log2) – – – 0.05 -0.01 -0.22 -0.3 -0.26 – -0.34 -0.32 -0.39 0.54 0.54 0.66 0.03 0.09 0.36 0.49 0.55 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.7 – 0 3.8 3.3 0 0 0 0 0 0
Cytoplasm 0.6 0.6 1.5 3.5 2.1 – 3.9 6.4 4.6 1.6 1.6 -0.1 2.9 3.9 0.5
Endoplasmic Reticulum 0 0 3.0 3.9 5.8 – 5.8 5.4 5.7 0 0 0 2.4 4.1 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 -0.3 0 0
Golgi 2.5 3.5 0.8 0.8 0.3 – -1.5 -1.1 -1.4 0 0 1.4 -0.8 1.3 3.9
Mitochondria 5.4 5.2 8.6 11.0 10.4 – 12.4 12.4 13.8 -2.8 -2.8 -3.1 -0.5 -0.2 -0.3
Nucleus -1.1 -0.8 -1.5 -1.9 -2.4 – -2.4 -1.9 -1.8 -0.2 -0.2 -0.5 1.7 1.9 1.5
Nuclear Periphery -0.1 -4.5 -5.2 -4.1 -6.6 – -7.4 -8.6 -7.0 -4.6 -4.1 -3.5 -3.0 -1.8 -0.9
Nucleolus -1.4 -3.3 -3.6 -4.1 -3.7 – -5.0 -4.7 -4.6 -1.0 -1.0 -2.7 -2.9 -1.4 -2.2
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.2 -1.6 -5.1 -8.3 -9.9 – -9.7 -12.0 -11.7 0.9 1.1 2.6 0 -1.3 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.014 0 0.0085 0.0145 0 0.0066 0.0078 0 0.0104 0.0134 0.021 0.0005 – – – – – –
Bud 0.0003 0.0001 0.0005 0.0001 0.0001 0.0011 0.0003 0.0003 0.0056 0.0002 0.0003 0.0005 – – – – – –
Bud Neck 0.0001 0 0.0006 0.0014 0 0.0008 0.0001 0 0.0005 0.0002 0.0001 0.0002 – – – – – –
Bud Periphery 0.0005 0.0001 0.0007 0.0002 0.0005 0.0021 0.0007 0.0002 0.0114 0.0008 0.0006 0.0025 – – – – – –
Bud Site 0.0003 0.0001 0.0011 0.0006 0 0.0018 0.0006 0.0001 0.0109 0.0003 0.0007 0.0004 – – – – – –
Cell Periphery 0.0008 0.0006 0.001 0.0003 0.0002 0.0007 0.001 0.0004 0.0017 0.0009 0.0017 0.0008 – – – – – –
Cytoplasm 0.0016 0.0011 0.004 0.0002 0 0.0006 0.0218 0.0028 0.0076 0.0004 0.0058 0.0054 – – – – – –
Cytoplasmic Foci 0.0005 0.0001 0.0016 0.0078 0 0.0008 0.007 0 0.0074 0.0054 0.0058 0.0034 – – – – – –
Eisosomes 0.0001 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0001 0.0001 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0031 0.0001 0.003 0.0001 0.0001 0.0012 0.0036 0.0003 0.002 0.0006 0.0026 0.0012 – – – – – –
Endosome 0.0138 0.0049 0.0275 0.0057 0.0018 0.0107 0.0156 0.0026 0.026 0.0142 0.0184 0.0118 – – – – – –
Golgi 0.0019 0.0001 0.0023 0.007 0 0.0019 0.003 0 0.0041 0.0052 0.0095 0.0014 – – – – – –
Lipid Particles 0.0012 0.0006 0.0017 0.0139 0 0.001 0.0177 0.0001 0.0071 0.0143 0.0044 0.0053 – – – – – –
Mitochondria 0.0031 0.0009 0.0057 0.0021 0.0001 0.009 0.0049 0.0062 0.0169 0.0323 0.0056 0.0017 – – – – – –
None 0.001 0 0.0003 0 0 0.0002 0.0052 0.0001 0.0012 0.0006 0.0007 0.0091 – – – – – –
Nuclear Periphery 0.0324 0.0133 0.0206 0.0005 0.0014 0.0321 0.045 0.011 0.006 0.003 0.0098 0.0296 – – – – – –
Nucleolus 0.0021 0.001 0.002 0.0002 0.0001 0.0019 0.0017 0.0002 0.0024 0.0007 0.0135 0.001 – – – – – –
Nucleus 0.0096 0.0119 0.0087 0.0003 0.0002 0.0037 0.0076 0.0039 0.002 0.0008 0.0057 0.0028 – – – – – –
Peroxisomes 0.0001 0 0.0002 0.0346 0 0.0003 0.0066 0 0.0036 0.0059 0.001 0.0024 – – – – – –
Punctate Nuclear 0.0005 0 0.0002 0.0003 0 0.0003 0.0082 0 0.0026 0.0033 0.0001 0.0014 – – – – – –
Vacuole 0.294 0.3287 0.3111 0.3643 0.2118 0.2614 0.2333 0.2398 0.2853 0.3151 0.2393 0.2319 – – – – – –
Vacuole Periphery 0.6192 0.6364 0.5987 0.546 0.7837 0.6616 0.6082 0.732 0.5853 0.5823 0.6531 0.6868 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.2521 70.2695 85.1348 100.7659 76.7523 71.0463 92.4301 102.4934 94.9107 106.9546
Translational Efficiency 0.4915 0.4922 0.5345 0.3953 0.4448 0.5523 0.4441 0.3269 0.3994 0.376

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
115 1249 1537 73 1904 2059 1872 1484 2019 3308 3409 1557

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1267.50 1363.84 1147.45 1543.54 1071.37 1346.48 1367.55 1268.92 1082.54 1353.03 1268.31 1281.80
Standard Deviation 203.56 270.45 279.82 294.08 205.97 255.68 247.62 240.99 210.79 261.49 284.55 250.55
Intensity Change Log 2 — 0.105689 -0.143554 0.284257 — 0.329736 0.352137 0.244144 — 0.212652 0.104747 0.266021

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000093 0.000054 0.000153 0.000037 0.000011 0.000032 0.000024 0.000067 0.000016 0.000040 0.000082 0.000065
Bud Neck 0.001385 0.000606 0.001260 0.001570 0.000151 0.000265 0.000590 0.001408 0.000221 0.000394 0.000892 0.001415
Bud Site 0.001933 0.000890 0.003537 0.001830 0.001006 0.000616 0.000504 0.002267 0.001058 0.000719 0.001872 0.002247
Cell Periphery 0.000498 0.000643 0.000746 0.000219 0.001244 0.000639 0.001276 0.000526 0.001202 0.000640 0.001037 0.000511
Cytoplasm 0.000214 0.000183 0.001673 0.000165 0.000280 0.000078 0.000071 0.000402 0.000277 0.000118 0.000793 0.000391
Cytoplasmic Foci 0.005983 0.000342 0.002011 0.001160 0.000170 0.000377 0.000372 0.001600 0.000502 0.000363 0.001111 0.001579
Eisosomes 0.000018 0.000015 0.000022 0.000008 0.000002 0.000016 0.000066 0.000024 0.000003 0.000016 0.000046 0.000023
Endoplasmic Reticulum 0.001130 0.000510 0.000427 0.000141 0.001288 0.000552 0.000357 0.000716 0.001279 0.000536 0.000389 0.000689
Endosome 0.016204 0.001837 0.005408 0.006015 0.000803 0.001244 0.001617 0.009264 0.001680 0.001468 0.003326 0.009112
Golgi 0.082712 0.000504 0.005191 0.032899 0.000290 0.000757 0.000831 0.007941 0.004985 0.000661 0.002797 0.009111
Lipid Particles 0.000708 0.000488 0.000737 0.000543 0.000133 0.000695 0.001446 0.001165 0.000166 0.000616 0.001127 0.001136
Mitochondria 0.028165 0.005629 0.011111 0.005015 0.003002 0.010387 0.008944 0.018428 0.004435 0.008590 0.009921 0.017799
Mitotic Spindle 0.000578 0.000312 0.000293 0.000111 0.000023 0.000074 0.000430 0.000367 0.000054 0.000164 0.000368 0.000355
None 0.000378 0.000523 0.001174 0.000132 0.000136 0.000387 0.000416 0.000508 0.000150 0.000438 0.000758 0.000490
Nuclear Periphery 0.001419 0.001539 0.001666 0.001468 0.000312 0.001988 0.001020 0.003492 0.000375 0.001818 0.001311 0.003397
Nuclear Periphery Foci 0.001095 0.000748 0.001133 0.000976 0.000091 0.000981 0.000627 0.002518 0.000149 0.000893 0.000855 0.002445
Nucleolus 0.002342 0.003130 0.005626 0.003113 0.000225 0.002791 0.004248 0.004001 0.000345 0.002919 0.004869 0.003959
Nucleus 0.001742 0.001866 0.004015 0.001138 0.000423 0.002224 0.001288 0.002963 0.000498 0.002089 0.002517 0.002877
Peroxisomes 0.000253 0.000434 0.000749 0.000254 0.000031 0.000201 0.000142 0.000230 0.000044 0.000289 0.000416 0.000232
Vacuole 0.390882* 0.225421* 0.208774* 0.352285* 0.124424 0.236403* 0.403662* 0.388137* 0.139601 0.232256* 0.315794* 0.386456*
Vacuole Periphery 0.462268* 0.754329* 0.744294* 0.590921* 0.865954* 0.739294* 0.572066* 0.553977* 0.842960* 0.744971* 0.649718* 0.555710*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.95 -1.50 1.59 0.75 4.10 -3.62 -2.92 -3.59 -2.18 -2.71 -2.74 -5.32 -3.33 -1.46 0.97
Bud Neck 1.36 0.15 -0.39 -1.84 -0.65 -1.64 -5.31 -7.11 -6.70 -4.66 -1.74 -6.05 -6.93 -5.80 -2.81
Bud Site 1.52 -1.83 -0.09 -1.39 1.55 1.25 1.95 -3.05 -4.27 -5.06 1.18 -2.22 -3.02 -4.24 -0.92
Cell Periphery -0.83 -1.22 1.60 2.87 2.91 3.49 -0.16 4.07 0.95 5.02 3.54 0.95 4.13 1.23 4.18
Cytoplasm 0.31 -4.03 0.27 -0.04 4.12 2.38 2.04 -1.16 -2.12 -2.06 1.92 -3.06 -1.16 -1.95 1.24
Cytoplasmic Foci 2.32 1.62 1.95 -2.15 1.76 -1.18 -3.14 -3.78 -2.97 -3.23 0.76 -3.13 -2.80 -3.24 -1.25
Eisosomes 0.66 -1.01 2.42 2.80 5.17 -9.12 -13.11 -10.64 -3.14 7.97 -10.61 -15.06 -10.25 -3.32 6.64
Endoplasmic Reticulum 1.26 1.42 2.02 5.87 5.21 4.22 5.89 3.25 -1.51 -4.46 4.66 5.83 3.50 -1.68 -3.81
Endosome 3.71 2.76 2.43 -2.85 -0.47 -2.06 -3.09 -10.48 -9.75 -9.13 0.67 -4.02 -9.22 -9.67 -6.93
Golgi 4.16 3.92 2.11 -2.42 -2.06 -1.97 -5.86 -6.88 -6.33 -6.38 3.58 1.77 -2.39 -6.79 -4.97
Lipid Particles 0.65 -0.11 0.39 -0.18 0.66 -6.91 -12.05 -6.35 -2.65 1.36 -6.54 -11.33 -6.20 -3.12 -0.11
Mitochondria 3.14 2.37 3.17 0.31 2.77 -4.95 -4.23 -7.80 -3.55 -4.29 -3.41 -4.65 -6.92 -4.63 -4.00
Mitotic Spindle 0.71 0.79 1.59 0.98 1.94 -1.77 -1.30 -2.83 -2.38 0.18 -1.40 -1.84 -2.58 -1.41 0.10
None -0.93 -4.63 3.44 2.74 6.47 -4.75 -8.22 -4.52 -1.29 -1.08 -4.52 -8.02 -4.34 -0.54 2.53
Nuclear Periphery -0.19 -0.52 -0.08 0.11 0.42 -5.22 -5.50 -4.85 -2.07 -3.72 -5.23 -6.49 -4.82 -2.32 -3.27
Nuclear Periphery Foci 0.99 -0.22 0.30 -0.69 0.59 -3.36 -8.51 -5.04 -2.81 -3.90 -4.05 -7.63 -5.00 -3.15 -3.38
Nucleolus -0.52 -2.21 -0.45 0.02 2.02 -5.45 -8.02 -6.84 -1.68 0.32 -6.27 -10.98 -6.77 -1.58 1.39
Nucleus -0.12 -1.94 0.51 1.23 3.76 -3.00 -5.10 -4.84 -1.06 -3.32 -3.81 -6.13 -4.73 -1.37 -0.66
Peroxisomes -0.68 -2.10 0.01 0.70 2.18 -2.21 -4.02 -6.22 -0.37 -2.25 -2.38 -3.97 -5.92 0.55 1.91
Vacuole 6.71 7.43 0.94 -4.87 -5.51 -18.15 -41.08 -35.84 -19.80 1.90 -16.45 -29.89 -33.58 -22.14 -9.82
Vacuole Periphery -11.08 -10.68 -3.09 5.37 5.02 18.79* 41.02* 38.65* 21.88 2.09 15.56 29.51 35.05 24.55 11.82
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR
Localization
Cell Percentages vacuolar membrane (89%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Ycf1

Ycf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available