Standard name
Human Ortholog
Description Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.06 0.07 0.11 0.08 – 0.12 0.2 0.14 0.08 0.08 0 0.1 0.13 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.1 – 0.1 0.09 0.1 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.06 0.08 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Mitochondria 0 0.05 0.05 0.18 0.18 0.28 0.37 0.33 – 0.43 0.44 0.49 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.06 0.09 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.08 0.07 0 0.05 0 0.06 0.05 0
Nuclear Periphery 0.07 0.07 0.3 0.29 0.16 0.15 0.18 0.14 – 0.1 0.07 0.11 0.12 0.14 0.18 0.2 0.23 0.26 0.05 0 0.15 0.09 0.07 0.07
Nucleolus 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.89 0.86 0.8 0.76 0.75 0.64 0.53 0.49 – 0.48 0.38 0.4 0.83 0.84 0.88 0.8 0.75 0.78 0.88 0.87 0.77 0.78 0.78 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 11 – 0 2 2 0 0 0 0 0 0 2 0 1 0 0 0
Bud 0 0 0 0 0 0 1 1 – 2 1 0 0 0 0 0 0 0 6 2 5 1 3 7
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 3 8 7 16 – 11 17 15 1 1 0 3 0 3 0 0 0 0 0 0
Cytoplasm 16 14 17 19 19 41 62 64 – 66 86 71 15 15 12 37 33 12 2 0 1 0 0 0
Endoplasmic Reticulum 0 0 2 4 5 21 29 78 – 54 39 49 2 1 4 10 15 9 1 0 1 1 3 2
Endosome 4 3 6 3 2 2 1 5 – 0 0 0 3 3 0 5 2 3 5 7 4 7 9 15
Golgi 30 5 8 20 27 18 17 20 – 5 5 5 5 4 11 5 10 20 0 0 0 3 1 2
Mitochondria 8 24 19 61 60 161 206 266 – 229 189 245 1 1 2 16 12 10 7 4 6 4 4 6
Nucleus 45 47 15 9 10 14 11 14 – 8 8 10 7 7 9 25 19 15 20 29 7 22 18 17
Nuclear Periphery 52 33 109 100 53 90 101 108 – 55 29 55 23 26 47 72 57 56 42 18 58 31 26 33
Nucleolus 22 9 21 12 4 9 6 14 – 2 1 3 7 7 4 6 8 4 12 4 1 7 10 14
Peroxisomes 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 – 0 0 0 1 1 0 0 0 0 0 1 0 0 0 1
Vac/Vac Membrane 696 430 293 259 255 373 296 393 – 256 163 202 156 156 234 287 187 168 744 474 299 288 286 398
Unique Cell Count 779 501 367 340 341 581 557 800 536 433 505 188 186 267 360 248 215 850 548 390 372 369 505
Labelled Cell Count 873 566 490 487 438 739 740 991 689 540 657 221 222 323 466 343 300 850 548 390 372 369 505


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.0 14.6 11.5 11.8 11.4 9.8 9.3 9.6 – 9.1 9.2 8.7 16.7 16.7 18.1 11.7 12.3 14.7 16.1 16.7 18.5
Std Deviation (1e-4) 4.5 4.7 3.6 3.3 3.2 3.1 3.1 2.7 – 2.7 2.6 2.3 5.2 5.2 5.3 3.8 4.4 5.3 4.9 5.1 5.8
Intensity Change (Log2) – – – 0.05 -0.01 -0.22 -0.3 -0.26 – -0.34 -0.32 -0.39 0.54 0.54 0.66 0.03 0.09 0.36 0.49 0.55 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.7 – 0 3.8 3.3 0 0 0 0 0 0
Cytoplasm 0.6 0.6 1.5 3.5 2.1 – 3.9 6.4 4.6 1.6 1.6 -0.1 2.9 3.9 0.5
Endoplasmic Reticulum 0 0 3.0 3.9 5.8 – 5.8 5.4 5.7 0 0 0 2.4 4.1 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 -0.3 0 0
Golgi 2.5 3.5 0.8 0.8 0.3 – -1.5 -1.1 -1.4 0 0 1.4 -0.8 1.3 3.9
Mitochondria 5.4 5.2 8.6 11.0 10.4 – 12.4 12.4 13.8 -2.8 -2.8 -3.1 -0.5 -0.2 -0.3
Nucleus -1.1 -0.8 -1.5 -1.9 -2.4 – -2.4 -1.9 -1.8 -0.2 -0.2 -0.5 1.7 1.9 1.5
Nuclear Periphery -0.1 -4.5 -5.2 -4.1 -6.6 – -7.4 -8.6 -7.0 -4.6 -4.1 -3.5 -3.0 -1.8 -0.9
Nucleolus -1.4 -3.3 -3.6 -4.1 -3.7 – -5.0 -4.7 -4.6 -1.0 -1.0 -2.7 -2.9 -1.4 -2.2
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.2 -1.6 -5.1 -8.3 -9.9 – -9.7 -12.0 -11.7 0.9 1.1 2.6 0 -1.3 -0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.014 0 0.0085 0.0145 0 0.0066 0.0078 0 0.0104 0.0134 0.021 0.0005 – – – – – –
Bud 0.0003 0.0001 0.0005 0.0001 0.0001 0.0011 0.0003 0.0003 0.0056 0.0002 0.0003 0.0005 – – – – – –
Bud Neck 0.0001 0 0.0006 0.0014 0 0.0008 0.0001 0 0.0005 0.0002 0.0001 0.0002 – – – – – –
Bud Periphery 0.0005 0.0001 0.0007 0.0002 0.0005 0.0021 0.0007 0.0002 0.0114 0.0008 0.0006 0.0025 – – – – – –
Bud Site 0.0003 0.0001 0.0011 0.0006 0 0.0018 0.0006 0.0001 0.0109 0.0003 0.0007 0.0004 – – – – – –
Cell Periphery 0.0008 0.0006 0.001 0.0003 0.0002 0.0007 0.001 0.0004 0.0017 0.0009 0.0017 0.0008 – – – – – –
Cytoplasm 0.0016 0.0011 0.004 0.0002 0 0.0006 0.0218 0.0028 0.0076 0.0004 0.0058 0.0054 – – – – – –
Cytoplasmic Foci 0.0005 0.0001 0.0016 0.0078 0 0.0008 0.007 0 0.0074 0.0054 0.0058 0.0034 – – – – – –
Eisosomes 0.0001 0 0.0001 0.0001 0 0.0001 0.0003 0 0.0001 0.0001 0.0001 0 – – – – – –
Endoplasmic Reticulum 0.0031 0.0001 0.003 0.0001 0.0001 0.0012 0.0036 0.0003 0.002 0.0006 0.0026 0.0012 – – – – – –
Endosome 0.0138 0.0049 0.0275 0.0057 0.0018 0.0107 0.0156 0.0026 0.026 0.0142 0.0184 0.0118 – – – – – –
Golgi 0.0019 0.0001 0.0023 0.007 0 0.0019 0.003 0 0.0041 0.0052 0.0095 0.0014 – – – – – –
Lipid Particles 0.0012 0.0006 0.0017 0.0139 0 0.001 0.0177 0.0001 0.0071 0.0143 0.0044 0.0053 – – – – – –
Mitochondria 0.0031 0.0009 0.0057 0.0021 0.0001 0.009 0.0049 0.0062 0.0169 0.0323 0.0056 0.0017 – – – – – –
None 0.001 0 0.0003 0 0 0.0002 0.0052 0.0001 0.0012 0.0006 0.0007 0.0091 – – – – – –
Nuclear Periphery 0.0324 0.0133 0.0206 0.0005 0.0014 0.0321 0.045 0.011 0.006 0.003 0.0098 0.0296 – – – – – –
Nucleolus 0.0021 0.001 0.002 0.0002 0.0001 0.0019 0.0017 0.0002 0.0024 0.0007 0.0135 0.001 – – – – – –
Nucleus 0.0096 0.0119 0.0087 0.0003 0.0002 0.0037 0.0076 0.0039 0.002 0.0008 0.0057 0.0028 – – – – – –
Peroxisomes 0.0001 0 0.0002 0.0346 0 0.0003 0.0066 0 0.0036 0.0059 0.001 0.0024 – – – – – –
Punctate Nuclear 0.0005 0 0.0002 0.0003 0 0.0003 0.0082 0 0.0026 0.0033 0.0001 0.0014 – – – – – –
Vacuole 0.294 0.3287 0.3111 0.3643 0.2118 0.2614 0.2333 0.2398 0.2853 0.3151 0.2393 0.2319 – – – – – –
Vacuole Periphery 0.6192 0.6364 0.5987 0.546 0.7837 0.6616 0.6082 0.732 0.5853 0.5823 0.6531 0.6868 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.2521 70.2695 85.1348 100.7659 76.7523 71.0463 92.4301 102.4934 94.9107 106.9546
Translational Efficiency 0.4915 0.4922 0.5345 0.3953 0.4448 0.5523 0.4441 0.3269 0.3994 0.376

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR
Localization
Cell Percentages vacuolar membrane (89%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Ycf1

Ycf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ycf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available