Standard name
Human Ortholog
Description RNA polymerase I subunit A14
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0.05 0.06 0.06 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14 0.15 0.05 0 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.24 0.09 0.15 0.11 0.07 0.17 0.13 0.13 0.13 0 0.05 0 0.08 0.14 0.16 0 0 0 0 0 0
Nucleus 0.14 0.08 0.13 0.11 0.05 0 0 0 0.05 0 0.08 0.07 0 0.09 0.06 0.05 0.06 0.08 0.09 0.07 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.9 0.91 0.92 0.9 0.89 0.9 0.82 0.86 0.9 0.83 0.85 0.81 0.9 0.77 0.72 0.9 0.9 0.79 0.77 0.78 0.63 0.72 0.68 0.61
Peroxisomes 0 0 0 0 0 0.05 0.06 0.06 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0.1 0.22 0.11 0.23 0.33 0.41 0.35 0.34 0.39 0.36 0.35 0.14 0.12 0.16 0.08 0.06 0 0.08 0.09 0.24 0.12 0.2 0.2
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 1 1 0 3 11 6 1 5 4 2 0 0 1 0 0 0 4 7 2 1 2 3
Bud Neck 7 12 15 0 0 2 14 9 2 16 5 14 9 1 3 2 0 1 2 5 1 0 3 1
Bud Site 0 3 7 6 6 8 24 16 18 25 24 45 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 3 1 9 4 16 11 1 6 8 7 10 38 43 10 6 14 1 0 0 2 2 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0
Endosome 1 3 1 1 19 11 6 8 2 3 1 1 1 7 17 2 1 0 3 4 4 3 0 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 1 0 2
Mitochondria 13 12 15 48 27 54 56 44 58 56 55 73 7 15 8 17 25 19 1 2 0 0 2 1
Nucleus 61 35 23 22 17 16 22 26 16 18 35 38 9 25 18 10 11 10 41 30 8 5 7 7
Nuclear Periphery 4 3 3 0 0 0 0 1 0 0 2 2 0 0 3 0 1 0 1 0 0 1 0 1
Nucleolus 389 389 168 177 281 334 430 526 301 351 370 470 190 214 211 181 160 95 344 347 115 119 173 156
Peroxisomes 2 9 0 2 5 20 30 39 11 18 25 32 5 8 8 2 1 1 0 0 0 0 1 2
SpindlePole 40 44 40 21 74 121 213 216 112 166 155 205 30 34 47 17 11 2 36 40 44 19 51 50
Vac/Vac Membrane 0 2 0 0 1 0 1 2 0 3 0 0 1 3 2 1 2 2 1 4 1 3 5 7
Unique Cell Count 432 428 183 197 316 371 525 609 333 425 433 580 212 277 292 201 178 120 446 448 184 166 255 256
Labelled Cell Count 522 517 276 279 439 573 823 904 522 667 684 889 262 347 364 243 218 145 446 448 184 166 255 256


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.5 13.4 7.0 9.3 9.2 7.7 7.2 8.1 7.0 7.8 7.5 7.7 16.1 14.0 14.3 21.4 21.0 17.1 14.4 16.3 16.2
Std Deviation (1e-4) 3.2 4.6 2.1 2.9 2.7 2.2 2.5 2.7 2.1 2.8 2.6 2.7 7.5 6.2 8.4 9.9 9.0 6.1 5.8 5.5 4.6
Intensity Change (Log2) – – – 0.42 0.4 0.15 0.04 0.22 0.01 0.16 0.1 0.14 1.21 1.01 1.04 1.62 1.59 1.29 1.05 1.22 1.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -4.1 -5.2 -4.9 -3.3 -4.6 -4.6 -2.3 -4.5 -3.6 -1.6 -4.5 -4.0 -3.4 -3.9 -2.8
Bud Site -0.4 0 0 0.4 -0.8 0.8 1.0 0.9 1.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 1.7 4.5 4.7 1.8 0 3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.0 0 0 0 0 0 0 0 0 0 2.9 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.2 0.1 2.1 1.0 -0.4 2.9 1.8 1.6 1.6 -2.1 -1.2 -2.7 0.1 1.8 2.1
Nucleus -0.4 -2.8 -3.6 -4.0 -4.1 -3.2 -3.8 -1.7 -2.6 -3.0 -1.2 -2.4 -2.7 -2.1 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.7 -1.0 -0.7 -3.2 -2.0 -0.5 -2.9 -2.2 -3.4 -0.7 -4.1 -5.2 -0.6 -0.6 -3.2
Peroxisomes 0 0 3.2 3.3 3.5 0 2.8 3.3 3.2 0 0 0 0 0 0
SpindlePole -3.0 0.4 2.6 4.5 3.5 2.8 4.1 3.4 3.4 -2.0 -2.7 -1.6 -3.7 -4.3 -5.0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.8585 16.6073 15.2537 14.6382 11.5195 14.5298 16.414 17.3031 15.4892 15.1912 14.3001 15.195 19.3376 17.9109 17.8726 17.5784 16.1854 16.6244
Actin 0.0126 0 0.0025 0 0.0166 0.0012 0.0001 0 0.0045 0 0.0006 0.0013 0 0 0 0 0 0
Bud 0.0004 0 0.0001 0 0.002 0.0001 0.0002 0 0.0002 0 0 0 0 0 0 0 0 0
Bud Neck 0.0007 0.0001 0.0003 0 0.0009 0.0001 0.0001 0 0.0004 0 0.0001 0 0 0.0002 0 0.0001 0.0001 0.0001
Bud Periphery 0.0005 0 0.0001 0 0.003 0.0001 0.0002 0 0.0002 0 0 0.0001 0 0 0 0 0 0
Bud Site 0.0047 0.0001 0.0006 0 0.017 0.0002 0.0006 0 0.0059 0 0.0001 0 0.0002 0 0 0 0 0
Cell Periphery 0.0005 0.0002 0.0001 0.0001 0.0006 0.0001 0.0002 0 0.0002 0 0.0001 0 0 0 0 0.0001 0 0
Cytoplasm 0.0074 0 0.0033 0 0.0039 0.0004 0.0011 0 0.0033 0 0.0001 0.0001 0 0 0 0 0 0
Cytoplasmic Foci 0.0184 0.0001 0.0097 0.0003 0.0357 0.0228 0.0063 0.0001 0.0045 0 0.0145 0.0071 0.0018 0.0001 0.0001 0.0002 0.0009 0.0002
Eisosomes 0.0003 0 0 0 0.0002 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0044 0 0.0011 0 0.0016 0.0004 0.0003 0 0.0014 0 0 0.0001 0 0 0 0 0 0
Endosome 0.0234 0 0.0063 0 0.0575 0.001 0.0011 0 0.0027 0 0.0014 0.0005 0 0 0 0 0 0
Golgi 0.0051 0 0.0025 0 0.0224 0.0008 0.0002 0 0.0014 0 0.0008 0.0004 0 0 0 0 0.0001 0
Lipid Particles 0.0151 0.0002 0.0068 0.0007 0.0386 0.0115 0.0035 0.0001 0.0037 0 0.015 0.0023 0.0007 0.0001 0.0001 0.0003 0.0024 0.0003
Mitochondria 0.0041 0.0005 0.0018 0.0004 0.0474 0.007 0.0017 0 0.0028 0 0.0101 0.0017 0.0004 0.0001 0 0.0041 0.006 0.0001
None 0.0044 0 0.0022 0 0.0024 0.0022 0.006 0 0.001 0 0.0003 0.0003 0.0002 0 0 0 0 0
Nuclear Periphery 0.0275 0 0.0009 0 0.003 0.0095 0.0031 0 0.0004 0 0.0002 0.0014 0 0.0002 0 0 0.0002 0
Nucleolus 0.7798 0.8769 0.872 0.9361 0.6768 0.889 0.914 0.9151 0.9059 0.9073 0.8998 0.9269 0.9434 0.8783 0.942 0.9371 0.8933 0.9353
Nucleus 0.0502 0.1171 0.0723 0.0622 0.0332 0.0446 0.0485 0.0817 0.0564 0.0902 0.0394 0.0546 0.0502 0.1184 0.0552 0.0576 0.0577 0.0571
Peroxisomes 0.0207 0 0.0009 0 0.0156 0.0004 0.0003 0 0.0024 0 0.0094 0.0002 0.0001 0 0 0 0.0003 0
Punctate Nuclear 0.0135 0.0001 0.0156 0.0001 0.0135 0.0057 0.0105 0.0026 0.0019 0.0022 0.0077 0.0022 0.0027 0.0024 0.0025 0.0004 0.0386 0.0068
Vacuole 0.0036 0.0014 0.0006 0.0001 0.0057 0.0004 0.0013 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0 0 0.0001 0 0
Vacuole Periphery 0.0027 0.0034 0.0003 0.0001 0.0025 0.0024 0.0006 0.0001 0.0002 0 0.0003 0.0005 0.0001 0 0 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 97.2808 78.6242 88.3978 79.7359 77.7507 47.9475 77.6901 59.3457 69.5943 64.4721
Translational Efficiency 1.8585 2.1283 1.6322 1.8848 1.9935 3.3971 1.7379 3.2137 2.1871 2.0681

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1705 1872 604 1348 2342 2325 2242 1490 4047 4197 2846 2838

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 895.44 1249.75 1238.54 1584.08 1050.99 1234.13 1718.92 1720.43 985.46 1241.10 1616.97 1655.67
Standard Deviation 186.12 246.17 248.88 424.13 219.49 236.43 361.35 466.54 219.94 240.95 393.18 452.05
Intensity Change Log 2 — 0.480971 0.467972 0.822977 — 0.231745 0.709753 0.711020 — 0.351765 0.603528 0.763606

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000312 0.000250 0.000273 0.000562 0.000242 0.000259 0.000404 0.000464 0.000272 0.000255 0.000376 0.000510
Bud Neck 0.002693 0.001661 0.002776 0.007719 0.001121 0.001546 0.001989 0.004135 0.001783 0.001597 0.002156 0.005837
Bud Site 0.001903 0.000612 0.000688 0.002677 0.000277 0.000608 0.000540 0.001386 0.000962 0.000609 0.000571 0.001999
Cell Periphery 0.000141 0.000024 0.000020 0.000119 0.000023 0.000036 0.000038 0.000107 0.000073 0.000031 0.000034 0.000113
Cytoplasm 0.002400 0.000452 0.000084 0.001384 0.000140 0.000223 0.000096 0.000785 0.001092 0.000325 0.000094 0.001069
Cytoplasmic Foci 0.006574 0.004494 0.002980 0.010467 0.002327 0.003265 0.002960 0.008964 0.004116 0.003813 0.002964 0.009678
Eisosomes 0.000323 0.000313 0.000178 0.000164 0.000337 0.000402 0.000356 0.000261 0.000331 0.000362 0.000318 0.000214
Endoplasmic Reticulum 0.001253 0.000303 0.000391 0.000371 0.000438 0.000380 0.000104 0.000174 0.000781 0.000346 0.000165 0.000267
Endosome 0.001081 0.000265 0.000119 0.000958 0.000149 0.000521 0.000149 0.001198 0.000542 0.000407 0.000143 0.001084
Golgi 0.001177 0.000409 0.000625 0.001160 0.000376 0.000412 0.000209 0.001243 0.000713 0.000411 0.000297 0.001203
Lipid Particles 0.000439 0.000339 0.000061 0.000178 0.000146 0.001043 0.000156 0.000263 0.000270 0.000729 0.000136 0.000222
Mitochondria 0.001218 0.001255 0.001093 0.003045 0.001340 0.001290 0.002032 0.004370 0.001289 0.001275 0.001833 0.003741
Mitotic Spindle 0.012092 0.014684 0.040711 0.046207 0.022375 0.011098 0.012205 0.020466 0.018043 0.012697 0.018255 0.032693
None 0.003908 0.000377 0.000326 0.002125 0.000394 0.000474 0.000306 0.000785 0.001874 0.000431 0.000310 0.001421
Nuclear Periphery 0.000168 0.000168 0.000220 0.000567 0.000341 0.000157 0.000200 0.000359 0.000268 0.000162 0.000204 0.000458
Nuclear Periphery Foci 0.006013 0.001055 0.001011 0.001018 0.001613 0.001441 0.000132 0.000310 0.003467 0.001269 0.000319 0.000646
Nucleolus 0.677519* 0.672733* 0.679724* 0.635811* 0.719688* 0.694713* 0.700957* 0.702526* 0.701923* 0.684909* 0.696451* 0.670838*
Nucleus 0.172563* 0.143866* 0.139651* 0.183367* 0.129258 0.170329* 0.162529* 0.163379* 0.147503* 0.158525* 0.157674* 0.172873*
Peroxisomes 0.106497* 0.155782* 0.127957* 0.097469 0.118628* 0.110617* 0.112992* 0.084722 0.113517* 0.130762* 0.116168* 0.090776
Vacuole 0.001237 0.000521 0.000646 0.002370 0.000474 0.000671 0.000741 0.001964 0.000796 0.000604 0.000720 0.002157
Vacuole Periphery 0.000488 0.000439 0.000465 0.002265 0.000312 0.000513 0.000908 0.002140 0.000386 0.000480 0.000814 0.002199

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.15 1.32 -7.35 -10.11 -9.49 -1.49 -10.07 -8.53 -7.31 -2.58 0.81 -6.48 -11.45 -12.39 -7.22
Bud Neck 2.77 -0.53 -10.21 -13.16 -7.78 -3.93 -6.49 -11.89 -9.59 -7.65 0.75 -2.36 -14.91 -16.09 -12.93
Bud Site 2.88 3.14 -2.77 -7.69 -8.64 -2.69 -3.21 -5.52 -3.40 -4.11 1.25 2.04 -5.96 -7.72 -8.89
Cell Periphery 3.55 3.71 0.38 -7.46 -7.95 -1.63 -1.41 -3.75 -3.19 -2.96 2.82 2.36 -2.43 -6.20 -5.42
Cytoplasm 2.60 3.57 1.37 -2.24 -4.86 -1.08 0.42 -2.42 -2.23 -2.51 2.33 3.56 -0.72 -3.18 -4.41
Cytoplasmic Foci 2.32 5.04 -4.44 -6.61 -8.96 -2.35 -1.52 -6.32 -5.31 -5.52 0.31 2.34 -8.08 -8.17 -9.13
Eisosomes 0.69 10.35 11.50 12.08 1.23 -3.23 -1.03 3.86 6.02 4.46 -2.35 1.01 9.20 10.48 7.14
Endoplasmic Reticulum 11.18 10.34 9.23 -2.18 -0.73 0.75 11.00 7.74 3.40 -3.68 8.29 16.29 11.01 1.08 -5.06
Endosome 2.09 2.58 0.37 -2.22 -2.93 -1.72 -0.06 -2.58 -1.44 -2.53 0.64 2.27 -1.72 -2.32 -3.58
Golgi 4.67 3.51 0.21 -2.52 -1.78 -0.39 2.86 -2.09 -1.99 -2.56 3.53 5.54 -1.75 -3.03 -3.57
Lipid Particles 0.31 2.30 1.52 1.50 -1.68 -1.99 -0.07 -1.16 1.73 -0.77 -1.78 1.29 0.67 2.06 -0.84
Mitochondria -0.19 0.66 -8.33 -7.40 -9.82 0.16 -2.91 -3.37 -3.42 -2.59 0.04 -3.17 -5.24 -5.22 -4.05
Mitotic Spindle -1.07 -6.10 -9.20 -8.56 -1.14 5.25 4.73 -0.20 -4.30 -3.89 3.65 -0.19 -6.88 -9.42 -6.41
None 4.58 4.66 2.01 -3.21 -3.32 -0.99 2.36 -4.48 -2.69 -5.56 4.37 4.79 1.17 -3.76 -4.32
Nuclear Periphery -0.03 -1.52 -7.36 -7.56 -6.59 1.71 1.28 -0.29 -4.57 -3.61 1.65 0.95 -3.14 -8.70 -7.43
Nuclear Periphery Foci 6.20 7.11 6.75 0.16 -0.05 0.26 6.80 5.89 3.31 -3.96 5.22 9.98 8.13 2.13 -2.05
Nucleolus 0.76 0.01 6.37 5.98 5.43 4.91 3.75 3.43 -0.16 0.64 4.29 1.42 7.43 4.30 5.91
Nucleus 5.37 4.69 -2.49 -6.48 -5.99 -9.82 -7.49 -6.13 1.04 -0.23 -3.36 -2.62 -6.37 -3.85 -3.72
Peroxisomes -11.01 -3.97 1.92 12.67 5.36 2.30 1.57 9.26 7.03 7.80 -6.18 -1.09 7.89 13.70 8.46
Vacuole 3.84 3.02 -2.78 -4.12 -3.91 -1.63 -1.52 -4.13 -3.48 -1.94 1.53 -0.53 -4.85 -5.37 -3.36
Vacuole Periphery 0.57 0.26 -4.03 -4.12 -4.08 -3.06 -10.22 -5.77 -5.02 -3.72 -2.02 -8.62 -6.80 -6.36 -5.02
Standard name
Human Ortholog
Description RNA polymerase I subunit A14
Localization
Cell Percentages nucleolus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Rpa14

Rpa14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpa14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available