Standard name
Human Ortholog
Description Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – 0 0 0.06 0 0 0 0 0 0 0 0.05 0.12 0.15 0.06 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0.07 0.05 0.07 0.16 0.16 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 – 0.95 0.91 0.89 0.85 0.83 0.82 0.81 0.78 0.82 0.81 0.93 0.85 0.79 0.88 0.86 0.89 0.93 0.94 0.94 0.89 0.79 0.63
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0.15 0.06 0.14 0.19 0.22 0.25 0.24 0.26 0.27 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0
Bud 1 – 0 0 0 0 1 2 1 7 1 4 0 0 0 0 0 0 4 0 4 0 2 4
Bud Neck 0 – 0 0 0 1 3 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 – 0 0 3 6 5 3 7 14 10 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 5 – 5 4 14 8 13 10 7 7 6 5 16 49 58 25 20 10 0 0 0 2 5 4
Endoplasmic Reticulum 0 – 0 0 0 0 2 1 0 0 0 0 1 0 0 5 1 0 0 0 0 1 0 0
Endosome 1 – 0 0 0 0 0 0 1 0 0 0 2 5 6 0 1 0 1 0 0 0 0 2
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 1
Mitochondria 2 – 0 2 1 9 32 16 29 80 54 42 0 0 0 3 2 3 1 0 0 0 1 0
Nucleus 348 – 246 86 192 220 364 257 347 400 278 286 304 358 307 340 249 236 347 42 248 169 292 251
Nuclear Periphery 0 – 0 0 2 0 2 2 3 6 0 2 0 0 1 0 1 0 0 0 0 1 1 0
Nucleolus 6 – 8 14 13 36 81 68 106 124 88 94 4 6 15 5 12 11 4 0 5 1 12 24
Peroxisomes 0 – 0 0 0 0 0 0 1 0 0 0 0 0 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 3 2 3 0 0 0 1 0 0 0 1 3
Vac/Vac Membrane 8 – 4 2 3 1 4 4 4 7 1 2 2 9 10 16 11 10 9 0 5 10 49 96
Unique Cell Count 367 258 95 216 258 436 314 430 511 341 354 328 423 389 386 289 265 375 45 266 191 370 397
Labelled Cell Count 371 263 108 228 281 507 363 508 647 438 441 334 430 404 394 298 270 375 45 266 191 370 397


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.1 – 15.9 12.9 11.2 9.1 8.3 7.9 7.5 6.9 6.4 6.6 17.9 17.4 15.3 21.4 20.2 19.8 18.0 18.1 18.8
Std Deviation (1e-4) 3.0 – 3.2 2.8 2.9 2.5 2.2 1.9 1.7 2.1 1.6 1.7 4.1 5.1 4.8 7.0 6.1 5.7 4.6 4.5 4.3
Intensity Change (Log2) – – – -0.3 -0.5 -0.8 -0.93 -1.01 -1.08 -1.19 -1.31 -1.27 0.17 0.13 -0.05 0.44 0.35 0.32 0.18 0.19 0.24

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70001020WT3HU80HU120HU16001020WT3rpd3Δ_1rpd3Δ_2rpd3Δ_301020WT1AF100AF140AF18001020
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.5 0.8 0.8 0.9 0 0 0 0 1.9 4.5 5.4 2.7 2.8 1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.5 3.7 4.3 6.7 6.7 5.7 0 0 0 0 0 0
Nucleus -1.7 -2.6 -3.9 -4.6 -4.9 -5.4 -6.1 -5.1 -5.3 -1.3 -4.3 -5.8 -3.2 -3.7 -2.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.0 1.5 4.4 5.9 6.5 7.4 7.3 7.5 7.7 -1.6 -1.5 0.5 -1.6 0.7 0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0.9 1.9 1.6 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8978 10.0871 9.3349 8.4432 7.4428 9.2396 8.9077 10.48 9.3163 9.2061 7.4379 9.4494 10.725 10.9537 9.8207 9.7033 7.7769 9.1018
Actin 0.0051 0.0001 0.0009 0 0.0001 0 0.0241 0 0.0104 0 0.0299 0.0003 0.025 0.0106 0.016 0 0.0038 0.0051
Bud 0.0005 0 0.0001 0.0001 0.0001 0 0.0008 0 0.0029 0 0.0021 0.0003 0.0003 0.0008 0.0001 0 0.0001 0.0002
Bud Neck 0.001 0 0.0001 0 0.0009 0.0003 0.001 0 0.0003 0.0001 0.0005 0.0007 0.0007 0.0002 0.0002 0 0.0012 0.0005
Bud Periphery 0.0005 0 0.0001 0 0.0003 0 0.0016 0 0.0054 0 0.0053 0.0005 0.0003 0.001 0.0002 0 0.0001 0.0004
Bud Site 0.0011 0 0.0002 0 0.0008 0 0.0022 0 0.0066 0 0.0039 0.0008 0.0011 0.0051 0.0006 0 0.0018 0.0002
Cell Periphery 0.0001 0 0.0001 0 0.0003 0 0.0006 0 0.0003 0 0.0003 0.0002 0.0002 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0081 0.0263 0.0008 0.0003 0.0012 0 0.0022 0 0.0022 0.0001 0.0003 0.0005 0.0083 0.0001 0.0007 0 0.0033 0.0046
Cytoplasmic Foci 0.0049 0 0.0011 0 0.0164 0 0.0056 0 0.0081 0 0.0026 0.0002 0.0107 0.0004 0.0056 0 0.0168 0.0011
Eisosomes 0.0002 0 0 0 0 0 0.0006 0 0.0001 0 0.0004 0 0.0001 0 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0044 0.0001 0.002 0 0.0012 0 0.0023 0 0.0014 0 0.0009 0.0002 0.0012 0 0.0015 0 0.0004 0.0006
Endosome 0.0044 0 0.0034 0 0.0129 0 0.0092 0 0.0062 0 0.0121 0.0003 0.0059 0.0005 0.0079 0 0.0074 0.0055
Golgi 0.0012 0 0.0006 0 0.0008 0 0.0039 0 0.0022 0 0.0092 0.0001 0.0027 0.0008 0.0042 0 0.0039 0.0028
Lipid Particles 0.001 0 0.0031 0 0.0145 0 0.0097 0 0.0075 0 0.0037 0.0001 0.0033 0.0002 0.0079 0 0.0072 0.0101
Mitochondria 0.0021 0.0002 0.001 0.0001 0.0008 0.0001 0.0091 0.0003 0.0058 0.0001 0.0952 0.0031 0.0018 0.001 0.0023 0.0001 0.0005 0.0187
None 0.007 0 0.0005 0 0.0008 0 0.0031 0 0.0023 0 0.0005 0.0001 0.0021 0 0.0004 0 0.0007 0.0005
Nuclear Periphery 0.0045 0.0058 0.004 0.0009 0.0069 0.0003 0.0052 0.0005 0.0058 0.0013 0.0017 0.0007 0.0068 0.0011 0.005 0.0022 0.0062 0.0112
Nucleolus 0.0049 0.0079 0.005 0.0033 0.064 0.0156 0.0113 0.0054 0.0082 0.0016 0.0186 0.0157 0.0133 0.0037 0.0028 0.0036 0.0161 0.0217
Nucleus 0.9393 0.9481 0.9744 0.9942 0.8549 0.9834 0.8828 0.9936 0.9154 0.9965 0.789 0.9749 0.8963 0.9693 0.9333 0.9938 0.8895 0.9068
Peroxisomes 0.0006 0 0.0007 0 0.0006 0 0.0134 0 0.0036 0 0.0102 0.0001 0.0034 0.0002 0.0075 0 0.0055 0.0018
Punctate Nuclear 0.0079 0.0111 0.0008 0.001 0.0056 0.0001 0.0038 0.0001 0.0025 0.0001 0.0047 0.0003 0.0149 0.0045 0.0023 0.0001 0.035 0.0061
Vacuole 0.001 0.0002 0.0007 0 0.0141 0.0001 0.0035 0 0.0019 0 0.0036 0.0006 0.0009 0.0001 0.0007 0 0.0003 0.0006
Vacuole Periphery 0.0004 0.0002 0.0004 0 0.0028 0 0.004 0.0001 0.0011 0 0.0053 0.0002 0.0006 0.0001 0.0005 0 0.0002 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.3205 46.5481 61.4057 60.0356 58.9391 41.3031 41.6485 33.0261 35.9262 60.2444
Translational Efficiency 1.182 1.1684 1.1475 0.914 1.0532 1.2948 0.9622 1.0948 0.9739 0.9483

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
89 145 1557 1342 1533 2660 409 98 1622 2805 1966 1440

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1099.54 1616.39 1605.56 1797.97 1271.53 1482.29 1733.59 1703.68 1262.09 1489.22 1632.19 1791.55
Standard Deviation 208.06 283.89 269.45 355.81 258.41 277.14 284.58 328.65 258.88 279.07 277.57 354.83
Intensity Change Log 2 — 0.555875 0.546176 0.709469 — 0.221262 0.447197 0.422089 — 0.386116 0.493943 0.562497

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002183 0.000346 0.000374 0.000532 0.000366 0.000463 0.000468 0.000865 0.000466 0.000457 0.000393 0.000555
Bud Neck 0.014335 0.004439 0.015320 0.012401 0.004640 0.008352 0.010451 0.008836 0.005172 0.008150 0.014307 0.012159
Bud Site 0.009067 0.000442 0.000869 0.001391 0.000365 0.001144 0.001215 0.003817 0.000843 0.001108 0.000941 0.001556
Cell Periphery 0.000166 0.000087 0.000125 0.000107 0.000233 0.000889 0.000142 0.000049 0.000230 0.000847 0.000129 0.000103
Cytoplasm 0.001835 0.000854 0.000345 0.000812 0.000627 0.000297 0.000290 0.003076 0.000693 0.000326 0.000333 0.000966
Cytoplasmic Foci 0.004061 0.000437 0.000011 0.001460 0.000008 0.000236 0.000043 0.009257 0.000230 0.000246 0.000018 0.001991
Eisosomes 0.000069 0.000050 0.000064 0.000045 0.000100 0.000232 0.000069 0.000051 0.000098 0.000222 0.000065 0.000046
Endoplasmic Reticulum 0.012397 0.001005 0.001278 0.000817 0.002592 0.004126 0.000758 0.001165 0.003130 0.003965 0.001170 0.000840
Endosome 0.003179 0.000611 0.000043 0.001849 0.000049 0.000330 0.000042 0.005156 0.000220 0.000344 0.000043 0.002074
Golgi 0.000653 0.000165 0.000017 0.001644 0.000013 0.000244 0.000025 0.000210 0.000048 0.000239 0.000018 0.001546
Lipid Particles 0.013051 0.000033 0.000006 0.000168 0.000005 0.000043 0.000008 0.000126 0.000721 0.000043 0.000006 0.000165
Mitochondria 0.006449 0.000883 0.001091 0.002856 0.000571 0.000881 0.001054 0.001991 0.000894 0.000881 0.001083 0.002797
Mitotic Spindle 0.013596 0.006041 0.000104 0.002401 0.000005 0.000778 0.000104 0.000903 0.000750 0.001050 0.000104 0.002299
None 0.000828 0.001802 0.000522 0.001108 0.001101 0.001142 0.000872 0.002222 0.001086 0.001176 0.000595 0.001184
Nuclear Periphery 0.000699 0.001561 0.000230 0.000668 0.000060 0.000150 0.000073 0.000257 0.000095 0.000223 0.000198 0.000640
Nuclear Periphery Foci 0.003517 0.000780 0.000021 0.001146 0.000019 0.000250 0.000003 0.000122 0.000211 0.000278 0.000017 0.001076
Nucleolus 0.014109 0.002726 0.001097 0.004064 0.004794 0.004364 0.003247 0.004662 0.005305 0.004279 0.001544 0.004105
Nucleus 0.891092 0.974723 0.978003 0.963153 0.983071 0.973453 0.980226 0.951701 0.978024 0.973519 0.978465 0.962374
Peroxisomes 0.003048 0.000257 0.000073 0.000277 0.000179 0.000156 0.000441 0.002385 0.000336 0.000162 0.000150 0.000420
Vacuole 0.004257 0.001625 0.000357 0.001953 0.001048 0.001945 0.000395 0.002950 0.001224 0.001929 0.000365 0.002021
Vacuole Periphery 0.001410 0.001130 0.000052 0.001148 0.000155 0.000524 0.000076 0.000199 0.000224 0.000555 0.000057 0.001083

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.88 2.80 2.48 -5.39 -5.23 -1.61 -3.59 -4.98 -4.18 -3.93 0.19 0.92 -3.25 -2.98 -5.66
Bud Neck 3.53 -0.67 -0.08 -10.35 1.42 -5.94 -3.77 -2.64 -1.68 -0.97 -4.79 -11.86 -10.83 -7.03 0.47
Bud Site 1.43 1.35 1.25 -3.32 -1.85 -3.84 -1.80 -1.69 -1.32 -1.23 -0.72 -0.50 -1.89 -1.62 -2.00
Cell Periphery 0.98 0.68 0.80 -0.51 0.40 -2.96 1.10 3.14 3.79 0.86 -2.94 2.08 2.46 3.62 0.60
Cytoplasm 1.17 1.93 1.05 -0.39 -2.70 0.84 0.12 -1.72 -2.06 -1.81 0.99 0.64 -1.15 -3.63 -3.07
Cytoplasmic Foci 1.65 1.86 1.29 -1.38 -2.85 -1.99 -2.79 -1.20 -1.17 -1.19 -0.09 1.71 -2.30 -2.28 -2.66
Eisosomes 2.06 0.77 2.70 0.76 4.69 -5.27 3.12 4.66 7.42 2.35 -5.22 3.93 6.14 7.87 5.55
Endoplasmic Reticulum 3.87 3.78 3.92 0.71 6.28 -5.87 9.63 4.65 10.21 -3.31 -2.74 8.01 9.19 15.98 4.76
Endosome 1.39 1.75 0.90 -1.36 -3.18 -3.10 0.05 -1.17 -1.08 -1.17 -0.95 1.75 -2.94 -2.69 -3.37
Golgi 1.66 2.32 -0.98 -1.82 -2.07 -1.45 -2.96 -1.91 0.22 -1.60 -1.26 1.43 -2.04 -1.67 -2.08
Lipid Particles 2.00 2.01 1.98 -2.21 -2.86 -2.26 -1.41 -1.61 -1.21 -1.43 1.87 1.96 1.53 -2.20 -2.98
Mitochondria 1.78 1.69 1.09 -3.06 -2.73 -1.09 -6.51 -5.56 -3.81 -3.98 0.04 -1.42 -3.28 -3.15 -2.91
Mitotic Spindle 0.75 1.51 1.19 0.68 -2.88 -1.99 -1.52 -1.70 -0.56 -0.47 -0.48 0.95 -1.99 -1.72 -2.78
None -0.85 1.15 -1.05 0.51 -2.62 -0.10 0.30 -1.47 -1.44 -1.70 -0.21 1.21 -0.54 -0.33 -2.69
Nuclear Periphery -0.59 1.69 -0.11 0.57 -2.39 -3.28 -2.03 -3.31 -2.40 -2.75 -1.60 -2.19 -3.50 -2.53 -2.65
Nuclear Periphery Foci 1.36 1.86 1.29 -0.27 -2.09 -1.18 0.37 -1.71 0.54 -1.79 -0.35 1.82 -1.63 -1.37 -2.11
Nucleolus 3.40 3.83 2.68 -3.13 -6.03 0.71 1.75 -0.86 -1.23 -1.86 1.84 8.27 0.15 -1.30 -5.61
Nucleus -3.18 -3.42 -2.74 1.58 4.61 5.01 1.87 2.65 2.00 2.26 1.90 0.93 5.34 4.19 5.17
Peroxisomes 1.91 2.03 1.89 -0.56 -4.61 0.52 -3.98 -1.81 -1.82 -1.55 1.94 2.07 -0.74 -2.79 -2.95
Vacuole 1.27 2.09 1.22 -0.30 -3.38 -2.66 1.45 -1.53 -1.10 -1.76 -2.08 2.80 -1.66 -0.35 -3.69
Vacuole Periphery 0.28 2.19 0.56 0.17 -3.13 -3.32 2.10 -0.42 2.45 -1.28 -2.87 3.92 -2.54 -1.29 -3.15
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Trm82

Trm82


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm82-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available