Standard name | |
---|---|
Human Ortholog | |
Description | Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 9 | 8 | 9 | 12 | 5 | – | 8 | 4 | 17 | 3 | 11 | 26 | 27 | 21 | 6 | 2 | 4 | 11 | 7 | 4 | 1 | 29 | 36 |
Bud | 1 | 1 | 3 | 12 | 21 | 18 | – | 23 | 32 | 40 | 41 | 44 | 6 | 6 | 8 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | – | 4 | 5 | 18 | 4 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 2 | 3 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1 | 0 | 7 | 8 | 7 | – | 9 | 4 | 18 | 13 | 10 | 13 | 17 | 21 | 5 | 4 | 8 | 0 | 0 | 0 | 0 | 1 | 2 |
Endoplasmic Reticulum | 1 | 0 | 5 | 2 | 1 | 1 | – | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 7 | 1 | 0 | 2 | 20 | 14 | – | 29 | 5 | 2 | 4 | 4 | 6 | 24 | 28 | 5 | 3 | 2 | 21 | 5 | 15 | 13 | 42 | 48 |
Golgi | 20 | 33 | 4 | 1 | 12 | 2 | – | 16 | 1 | 1 | 0 | 1 | 66 | 70 | 104 | 41 | 51 | 54 | 111 | 85 | 74 | 69 | 348 | 448 |
Mitochondria | 175 | 111 | 130 | 310 | 427 | 434 | – | 543 | 423 | 545 | 370 | 549 | 499 | 683 | 765 | 5 | 8 | 8 | 15 | 9 | 12 | 10 | 48 | 36 |
Nucleus | 1 | 0 | 0 | 0 | 2 | 0 | – | 5 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 5 | 0 | 3 | 1 | 3 | 7 | – | 11 | 11 | 6 | 10 | 11 | 8 | 6 | 5 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 25 | 3 | 4 | 21 | 21 | 34 | – | 97 | 59 | 42 | 48 | 57 | 14 | 24 | 32 | 5 | 14 | 9 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 2 | 2 | 2 | 37 | 67 | 79 | – | 142 | 41 | 99 | 68 | 112 | 18 | 59 | 89 | 6 | 21 | 8 | 25 | 8 | 27 | 11 | 36 | 34 |
SpindlePole | 1 | 0 | 0 | 5 | 12 | 11 | – | 35 | 21 | 28 | 25 | 27 | 1 | 2 | 4 | 3 | 4 | 4 | 3 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 3 | 5 | 4 | 5 | 5 | 1 | – | 11 | 5 | 2 | 4 | 5 | 21 | 11 | 26 | 5 | 5 | 6 | 2 | 3 | 0 | 3 | 6 | 10 |
Unique Cell Count | 188 | 121 | 135 | 336 | 483 | 481 | 740 | 493 | 628 | 449 | 655 | 528 | 745 | 871 | 71 | 100 | 97 | 193 | 122 | 135 | 112 | 519 | 627 | |
Labelled Cell Count | 247 | 167 | 163 | 413 | 612 | 617 | 935 | 615 | 821 | 595 | 854 | 682 | 931 | 1104 | 87 | 114 | 109 | 193 | 122 | 135 | 112 | 519 | 627 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 13.1 | 8.9 | 8.9 | 9.8 | 9.5 | – | 10.2 | 9.1 | 8.6 | 8.5 | 8.7 | 12.9 | 14.0 | 13.8 | 15.7 | 17.0 | 16.0 | 10.6 | 12.5 | 13.1 |
Std Deviation (1e-4) | 2.1 | 2.5 | 1.7 | 1.6 | 1.7 | 2.2 | – | 2.3 | 2.5 | 2.4 | 2.5 | 2.6 | 2.7 | 3.0 | 3.3 | 4.1 | 4.0 | 4.0 | 2.4 | 2.4 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.13 | 0.09 | – | 0.19 | 0.04 | -0.04 | -0.07 | -0.03 | 0.54 | 0.65 | 0.64 | 0.82 | 0.93 | 0.84 | 0.25 | 0.49 | 0.56 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -0.5 | -1.3 | 0 | 0 | 0 | -0.6 |
Bud | 0 | 1.1 | 0 | – | 0 | 1.9 | 1.9 | 2.7 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.2 | 2.5 | 3.1 | 9.0 | 8.6 | 9.1 |
Mitochondria | -1.6 | -2.7 | -2.2 | – | -5.8 | -3.3 | -3.1 | -4.0 | -3.8 | -0.8 | -1.9 | -2.9 | -12.8 | -13.6 | -13.5 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.4 | 0.7 | 1.8 | – | 3.4 | 3.1 | 1.6 | 2.8 | 2.3 | 0 | 0 | 0 | 0 | 3.1 | 2.1 |
Peroxisomes | 3.4 | 4.0 | 4.5 | – | 5.1 | 2.8 | 4.4 | 4.3 | 4.7 | 0 | 2.7 | 3.3 | 0 | 5.0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 2.6 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.6 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.8693 | 13.667 | 12.4424 | 11.5491 | 10.9191 | 13.3282 | 10.3568 | 12.6692 | 12.7521 | 12.0034 | 12.2834 | 12.7999 | 13.0089 | 15.4662 | 14.9417 | 12.9915 | 15.065 | 16.4676 |
Actin | 0.0256 | 0.0387 | 0.0351 | 0.015 | 0.0158 | 0.0257 | 0.0895 | 0.0289 | 0.0321 | 0.0241 | 0.0414 | 0.0529 | 0.0914 | 0.049 | 0.0472 | 0.0185 | 0.0044 | 0.0088 |
Bud | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0029 | 0.0011 | 0.0002 | 0.0001 | 0.0005 |
Bud Neck | 0.0024 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0008 | 0.0007 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0005 | 0.0006 | 0.0015 | 0.0002 | 0.0001 | 0.0015 |
Bud Periphery | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0025 | 0.0014 | 0.0004 | 0.0001 | 0.0009 |
Bud Site | 0.003 | 0.0008 | 0.0011 | 0.0017 | 0.0006 | 0.0001 | 0.0022 | 0.0044 | 0.0013 | 0.0002 | 0.0002 | 0.0002 | 0.002 | 0.0121 | 0.0184 | 0.0008 | 0.0001 | 0.001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0003 |
Cytoplasm | 0.002 | 0.0003 | 0.0004 | 0.0004 | 0.0122 | 0.0005 | 0.0009 | 0.0093 | 0.0115 | 0.0062 | 0.0004 | 0.0001 | 0.0072 | 0.0013 | 0.0005 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0621 | 0.0551 | 0.0474 | 0.0516 | 0.0585 | 0.0323 | 0.0457 | 0.0612 | 0.0424 | 0.0524 | 0.0377 | 0.0202 | 0.046 | 0.0456 | 0.0279 | 0.0151 | 0.0292 | 0.0174 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0027 | 0.0001 | 0.0003 | 0.0009 | 0.0017 | 0.0006 | 0.0025 | 0.0002 | 0.0024 | 0.0048 | 0.0007 | 0.0002 | 0.0014 | 0.0003 | 0.0009 | 0.0003 | 0.0001 | 0.0001 |
Endosome | 0.0948 | 0.0796 | 0.0711 | 0.0809 | 0.0741 | 0.0524 | 0.0673 | 0.1027 | 0.0913 | 0.1077 | 0.1238 | 0.0462 | 0.0752 | 0.112 | 0.0666 | 0.1101 | 0.0526 | 0.0343 |
Golgi | 0.5992 | 0.7021 | 0.6386 | 0.7479 | 0.6661 | 0.7426 | 0.4551 | 0.6198 | 0.6031 | 0.4925 | 0.7065 | 0.6559 | 0.4394 | 0.5309 | 0.5952 | 0.4676 | 0.7225 | 0.6542 |
Lipid Particles | 0.0754 | 0.0333 | 0.0926 | 0.0519 | 0.0287 | 0.0507 | 0.13 | 0.0853 | 0.0756 | 0.1895 | 0.0277 | 0.08 | 0.1137 | 0.0643 | 0.0747 | 0.0182 | 0.0382 | 0.1031 |
Mitochondria | 0.0691 | 0.049 | 0.074 | 0.018 | 0.0898 | 0.0534 | 0.1022 | 0.0392 | 0.0785 | 0.0729 | 0.0361 | 0.0972 | 0.1113 | 0.1217 | 0.0957 | 0.3343 | 0.1392 | 0.1458 |
None | 0.0019 | 0 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0017 | 0.0001 | 0.0009 | 0.0002 | 0 | 0 | 0.0067 | 0.0001 | 0.0005 | 0.0005 | 0 | 0 |
Nuclear Periphery | 0.005 | 0.0001 | 0.0008 | 0.0009 | 0.0045 | 0.0013 | 0.0228 | 0.0002 | 0.0011 | 0.0035 | 0.0002 | 0.0001 | 0.0159 | 0.0003 | 0.0008 | 0.0004 | 0.0001 | 0.0001 |
Nucleolus | 0.0021 | 0.0004 | 0.0004 | 0.0002 | 0.0013 | 0.0005 | 0.0006 | 0.0021 | 0.0009 | 0.0004 | 0 | 0 | 0.0008 | 0.0011 | 0.0002 | 0.0005 | 0 | 0.0002 |
Nucleus | 0.0012 | 0.0001 | 0.0003 | 0.0003 | 0.0032 | 0.0005 | 0.0016 | 0.0003 | 0.0003 | 0.0002 | 0 | 0 | 0.0088 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0001 |
Peroxisomes | 0.0381 | 0.034 | 0.0314 | 0.0234 | 0.0219 | 0.0326 | 0.0578 | 0.0341 | 0.0402 | 0.0332 | 0.0205 | 0.0427 | 0.046 | 0.0264 | 0.0566 | 0.0109 | 0.0087 | 0.0249 |
Punctate Nuclear | 0.0054 | 0.0001 | 0.001 | 0.0014 | 0.002 | 0.0034 | 0.0011 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0229 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.0042 | 0.0018 | 0.0014 | 0.0016 | 0.007 | 0.0006 | 0.0055 | 0.005 | 0.0086 | 0.0031 | 0.0011 | 0.0005 | 0.0024 | 0.0154 | 0.0017 | 0.004 | 0.0008 | 0.0015 |
Vacuole Periphery | 0.0045 | 0.0033 | 0.0027 | 0.002 | 0.0111 | 0.002 | 0.0104 | 0.0054 | 0.0077 | 0.0078 | 0.0025 | 0.0023 | 0.0066 | 0.0124 | 0.0078 | 0.0165 | 0.0033 | 0.0047 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 103.4785 | 100.437 | 101.1112 | 108.9456 | 88.9684 | 88.8819 | 111.4753 | 103.4806 | 97.3105 | 109.5617 |
Translational Efficiency | 0.989 | 1.0446 | 0.9238 | 0.8321 | 0.8836 | 0.9623 | 0.7823 | 0.6808 | 0.7526 | 0.6938 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
85 | 1546 | 1135 | 949 | 214 | 220 | 1696 | 953 | 299 | 1766 | 2831 | 1902 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1077.91 | 1163.75 | 1341.15 | 1342.59 | 1152.46 | 1200.27 | 1439.55 | 1286.50 | 1131.27 | 1168.30 | 1400.10 | 1314.49 |
Standard Deviation | 217.48 | 201.06 | 183.62 | 195.05 | 164.82 | 197.08 | 201.67 | 185.34 | 184.44 | 200.93 | 200.52 | 192.30 |
Intensity Change Log 2 | — | 0.110544 | 0.315234 | 0.316782 | — | 0.058642 | 0.320901 | 0.158735 | — | 0.083959 | 0.318165 | 0.237280 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.079547 | 0.043205 | 0.036474 | 0.030714 | 0.022790 | 0.012347 | 0.081939 | 0.033196 | 0.038925 | 0.039360 | 0.063711 | 0.031958 |
Bud Neck | 0.002364 | 0.000611 | 0.001574 | 0.002240 | 0.001389 | 0.000984 | 0.001314 | 0.001204 | 0.001666 | 0.000657 | 0.001418 | 0.001721 |
Bud Site | 0.000687 | 0.000575 | 0.001151 | 0.003238 | 0.000673 | 0.001061 | 0.000746 | 0.002180 | 0.000677 | 0.000636 | 0.000909 | 0.002708 |
Cell Periphery | 0.000150 | 0.000091 | 0.000089 | 0.000154 | 0.000142 | 0.000092 | 0.000081 | 0.000114 | 0.000145 | 0.000091 | 0.000084 | 0.000134 |
Cytoplasm | 0.000768 | 0.000820 | 0.000672 | 0.001206 | 0.000704 | 0.000745 | 0.000698 | 0.001103 | 0.000722 | 0.000811 | 0.000687 | 0.001154 |
Cytoplasmic Foci | 0.157347 | 0.144043 | 0.192772* | 0.170326 | 0.172576 | 0.231653* | 0.105812 | 0.189737* | 0.168247 | 0.154957 | 0.140676 | 0.180052* |
Eisosomes | 0.000925 | 0.000524 | 0.000258 | 0.000236 | 0.000450 | 0.000166 | 0.000720 | 0.000204 | 0.000585 | 0.000479 | 0.000535 | 0.000220 |
Endoplasmic Reticulum | 0.000909 | 0.000395 | 0.000598 | 0.000649 | 0.000723 | 0.000336 | 0.000514 | 0.000443 | 0.000776 | 0.000388 | 0.000548 | 0.000546 |
Endosome | 0.048267 | 0.054783 | 0.065219 | 0.121897 | 0.071126 | 0.127727 | 0.040022 | 0.153231 | 0.064628 | 0.063870 | 0.050124 | 0.137597 |
Golgi | 0.436312* | 0.541302* | 0.514149* | 0.499094* | 0.524621* | 0.469870* | 0.578276* | 0.498881* | 0.499516* | 0.532403* | 0.552566* | 0.498988* |
Lipid Particles | 0.105564* | 0.058820 | 0.128840* | 0.053382 | 0.058122 | 0.066517 | 0.125653* | 0.029931 | 0.071609 | 0.059779 | 0.126931* | 0.041632 |
Mitochondria | 0.141743* | 0.133070* | 0.032061 | 0.057474 | 0.117179* | 0.056365 | 0.044074 | 0.046700 | 0.124162* | 0.123515* | 0.039258 | 0.052076 |
Mitotic Spindle | 0.001094 | 0.000569 | 0.003850 | 0.010652 | 0.001847 | 0.001441 | 0.001760 | 0.006783 | 0.001633 | 0.000677 | 0.002598 | 0.008714 |
None | 0.000436 | 0.000207 | 0.000116 | 0.000134 | 0.000258 | 0.000087 | 0.000201 | 0.000083 | 0.000309 | 0.000192 | 0.000167 | 0.000109 |
Nuclear Periphery | 0.000711 | 0.000135 | 0.000744 | 0.000783 | 0.000209 | 0.000182 | 0.000750 | 0.000348 | 0.000352 | 0.000141 | 0.000748 | 0.000565 |
Nuclear Periphery Foci | 0.000489 | 0.000131 | 0.000712 | 0.001009 | 0.000334 | 0.000279 | 0.000656 | 0.000846 | 0.000378 | 0.000150 | 0.000678 | 0.000928 |
Nucleolus | 0.000095 | 0.000066 | 0.000038 | 0.000192 | 0.000094 | 0.000050 | 0.000040 | 0.000061 | 0.000095 | 0.000064 | 0.000039 | 0.000127 |
Nucleus | 0.000045 | 0.000034 | 0.000026 | 0.000060 | 0.000066 | 0.000041 | 0.000025 | 0.000029 | 0.000060 | 0.000035 | 0.000026 | 0.000044 |
Peroxisomes | 0.016295 | 0.016526 | 0.008681 | 0.015478 | 0.020188 | 0.024403 | 0.007465 | 0.017616 | 0.019081 | 0.017507 | 0.007953 | 0.016549 |
Vacuole | 0.003349 | 0.001640 | 0.006343 | 0.019089 | 0.003026 | 0.002678 | 0.004402 | 0.010879 | 0.003118 | 0.001770 | 0.005180 | 0.014975 |
Vacuole Periphery | 0.002901 | 0.002453 | 0.005630 | 0.011993 | 0.003482 | 0.002975 | 0.004853 | 0.006429 | 0.003317 | 0.002518 | 0.005164 | 0.009205 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.75 | 2.08 | 2.36 | 2.71 | 1.28 | 1.66 | -8.79 | -1.67 | -4.08 | 8.61 | -0.06 | -3.26 | 0.94 | 1.96 | 8.33 |
Bud Neck | 3.01 | 1.28 | 0.11 | -6.57 | -2.26 | 0.97 | 0.24 | 0.60 | -0.64 | 0.63 | 3.83 | 0.88 | -0.29 | -7.00 | -1.87 |
Bud Site | 0.55 | -2.05 | -7.48 | -8.15 | -6.10 | -0.79 | -0.38 | -4.57 | -2.02 | -5.10 | 0.20 | -1.45 | -8.23 | -8.74 | -8.26 |
Cell Periphery | 2.80 | 2.96 | -0.17 | -3.88 | -4.11 | 1.99 | 2.81 | 1.22 | -1.59 | -4.88 | 3.09 | 3.63 | 0.54 | -4.50 | -5.82 |
Cytoplasm | -0.68 | 1.15 | -3.99 | -4.41 | -5.72 | -0.27 | 0.09 | -4.28 | -2.01 | -5.29 | -1.61 | 0.66 | -5.99 | -5.45 | -7.83 |
Cytoplasmic Foci | 0.94 | -2.46 | -0.91 | -4.73 | 3.55 | -4.16 | 7.06 | -1.64 | 3.49 | -14.91 | 1.60 | 3.45 | -1.43 | -5.33 | -9.45 |
Eisosomes | 1.95 | 3.26 | 3.36 | 8.66 | 0.91 | 4.43 | -3.64 | 3.92 | -1.74 | 11.99 | 1.38 | 0.64 | 4.89 | 10.17 | 11.08 |
Endoplasmic Reticulum | 3.21 | 1.92 | 1.56 | -3.79 | -0.73 | 2.80 | 1.63 | 2.15 | -1.63 | 1.72 | 3.67 | 2.22 | 2.17 | -3.43 | 0.04 |
Endosome | -0.73 | -1.88 | -7.69 | -14.04 | -11.03 | -4.57 | 4.61 | -10.01 | -2.19 | -21.27* | 0.13 | 2.63 | -11.72 | -18.13 | -23.82 |
Golgi | -3.42 | -2.51 | -1.99 | 3.83 | 1.31 | 2.18 | -2.80 | 1.30 | -1.47 | 7.29 | -1.96 | -3.23 | 0.06 | 3.80 | 6.70 |
Lipid Particles | 2.53 | -1.21 | 2.79 | 1.03 | 10.52 | -0.64 | -7.37 | 3.39 | 3.44 | 17.94 | 1.42 | -6.46 | 3.69 | 4.57 | 19.38 |
Mitochondria | 0.34 | 4.43 | 3.38 | 9.82 | -4.52 | 3.42 | 4.78 | 4.53 | 0.92 | -0.52 | 0.05 | 6.54 | 5.48 | 11.21 | -3.38 |
Mitotic Spindle | 1.52 | -2.68 | -5.00 | -5.33 | -3.23 | 0.35 | 0.08 | -2.71 | -3.16 | -3.02 | 1.40 | -1.17 | -5.09 | -6.50 | -4.63 |
None | 3.61 | 5.06 | 4.77 | 6.21 | -1.80 | 5.88 | 2.09 | 6.68 | 0.26 | 11.33 | 4.29 | 5.31 | 7.57 | 9.04 | 7.67 |
Nuclear Periphery | 2.37 | -0.14 | -0.28 | -5.26 | -0.24 | 0.55 | -4.10 | -1.64 | -1.84 | 2.64 | 2.88 | -3.48 | -2.08 | -5.73 | 1.59 |
Nuclear Periphery Foci | 2.88 | -1.19 | -2.91 | -6.55 | -1.54 | 0.45 | -1.47 | -1.50 | -1.60 | -0.47 | 4.01 | -2.02 | -2.93 | -4.28 | -1.09 |
Nucleolus | 0.62 | 1.99 | -1.16 | -1.39 | -1.78 | 0.74 | 1.14 | 0.65 | -0.26 | -1.10 | 0.62 | 1.58 | -0.70 | -1.20 | -1.96 |
Nucleus | 0.73 | 3.19 | -1.11 | -1.40 | -2.36 | 0.86 | 2.02 | 1.75 | 0.55 | -0.64 | 1.33 | 2.36 | 0.85 | -0.75 | -2.43 |
Peroxisomes | -0.05 | 1.82 | 0.16 | 0.42 | -3.28 | -0.58 | 3.12 | 0.58 | 1.08 | -5.26 | 0.46 | 3.53 | 0.74 | 0.47 | -6.15 |
Vacuole | 1.91 | -2.84 | -8.43 | -10.49 | -7.23 | 0.39 | -1.68 | -6.04 | -7.00 | -5.69 | 2.23 | -3.11 | -10.34 | -13.16 | -9.38 |
Vacuole Periphery | 1.15 | -4.78 | -8.39 | -9.25 | -5.72 | 0.41 | -1.28 | -2.40 | -3.63 | -2.17 | 1.05 | -2.35 | -6.11 | -10.63 | -6.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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