Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by human homolog DCAKD and by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies; also localized to lipid droplets |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | – | 5 | 17 | 6 | 0 | 3 | 4 | 1 | 3 | 1 | 5 | 12 | 6 | 10 | 0 | 5 | 7 | 1 | 0 | 2 | 1 | 5 | 8 |
Bud | 0 | – | 1 | 5 | 0 | 1 | 10 | 6 | 13 | 7 | 16 | 11 | 1 | 2 | 1 | 0 | 2 | 3 | 2 | 0 | 0 | 1 | 4 | 4 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 8 | 6 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 3 | 0 | 8 | 6 | 2 | 5 | 0 | 2 | 3 | 1 | 3 | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 24 | – | 116 | 179 | 265 | 267 | 269 | 273 | 176 | 163 | 112 | 149 | 274 | 450 | 467 | 30 | 79 | 109 | 13 | 15 | 39 | 66 | 229 | 196 |
Endoplasmic Reticulum | 19 | – | 47 | 49 | 31 | 26 | 17 | 4 | 10 | 22 | 3 | 9 | 109 | 132 | 68 | 3 | 6 | 9 | 68 | 21 | 121 | 32 | 123 | 201 |
Endosome | 0 | – | 6 | 11 | 10 | 31 | 47 | 61 | 49 | 60 | 45 | 79 | 35 | 35 | 72 | 15 | 34 | 43 | 1 | 1 | 9 | 4 | 11 | 23 |
Golgi | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 3 | 2 | 0 | 1 | 0 | 1 | 5 |
Mitochondria | 0 | – | 1 | 9 | 3 | 26 | 48 | 30 | 184 | 142 | 162 | 194 | 1 | 3 | 2 | 3 | 5 | 8 | 10 | 4 | 42 | 13 | 37 | 34 |
Nucleus | 2 | – | 1 | 3 | 4 | 5 | 5 | 8 | 10 | 7 | 12 | 12 | 0 | 1 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 2 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 3 |
Nucleolus | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 2 | 4 | 0 | 5 | 8 | 5 | 7 | 3 | 4 | 4 | 0 | 3 | 2 | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 7 | – | 29 | 21 | 35 | 96 | 161 | 186 | 139 | 161 | 113 | 168 | 19 | 25 | 44 | 13 | 21 | 28 | 10 | 3 | 11 | 12 | 40 | 50 |
Unique Cell Count | 51 | 191 | 269 | 338 | 422 | 513 | 522 | 490 | 493 | 385 | 538 | 425 | 612 | 628 | 58 | 143 | 195 | 115 | 48 | 240 | 140 | 474 | 550 | |
Labelled Cell Count | 54 | 210 | 296 | 367 | 458 | 570 | 587 | 590 | 580 | 474 | 640 | 460 | 657 | 675 | 67 | 157 | 217 | 115 | 48 | 240 | 140 | 474 | 550 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | – | 7.2 | 6.9 | 7.4 | 6.3 | 6.2 | 6.7 | 5.6 | 5.9 | 5.9 | 6.0 | 7.1 | 7.3 | 7.1 | 6.8 | 8.3 | 8.7 | 8.0 | 7.9 | 8.3 |
Std Deviation (1e-4) | 0.9 | – | 1.4 | 1.4 | 1.5 | 1.3 | 1.5 | 2.2 | 1.1 | 1.1 | 3.4 | 1.0 | 1.0 | 0.9 | 0.9 | 1.3 | 1.6 | 1.9 | 1.5 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.02 | -0.21 | -0.22 | -0.12 | -0.38 | -0.3 | -0.29 | -0.28 | -0.02 | 0.01 | -0.02 | -0.09 | 0.19 | 0.26 | 0.14 | 0.12 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0.6 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 4.3 | 0.6 | -2.0 | -2.0 | -5.9 | -6.6 | -7.3 | -8.2 | 0.9 | 3.4 | 3.6 | -1.2 | -1.0 | -1.0 |
Endoplasmic Reticulum | -1.7 | -4.8 | -6.5 | -8.7 | -10.9 | -9.6 | -7.8 | -9.6 | -10.2 | 0.3 | -0.9 | -4.8 | -3.2 | -5.1 | -5.6 |
Endosome | 0.5 | -0.1 | 2.0 | 2.7 | 3.5 | 3.0 | 3.6 | 3.4 | 4.3 | 2.3 | 1.4 | 3.4 | 0 | 5.7 | 5.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.2 | 4.1 | 3.0 | 9.8 | 8.2 | 10.4 | 9.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.5 | -1.6 | 2.2 | 4.3 | 5.3 | 3.6 | 4.6 | 3.7 | 4.3 | -4.6 | -5.3 | -3.5 | 1.3 | -0.1 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7714 | 1.9453 | 1.7186 | 1.8705 | 1.4669 | 1.7965 | 3.4316 | 4.1744 | 3.4893 | 3.6711 | 3.008 | 3.5869 | 1.9865 | 2.6595 | 2.1133 | 2.0493 | 2.3437 | 2.1536 |
Actin | 0.0043 | 0.0052 | 0.0039 | 0.0037 | 0.002 | 0.0013 | 0.0198 | 0.0004 | 0.0172 | 0.0054 | 0.0042 | 0.004 | 0.0235 | 0.0016 | 0.0043 | 0.0358 | 0.0008 | 0.001 |
Bud | 0.0011 | 0.0005 | 0.0047 | 0.0071 | 0.0233 | 0.0012 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0003 | 0.0005 | 0.0004 | 0.0001 |
Bud Neck | 0.0003 | 0.0007 | 0.0005 | 0.0009 | 0.0005 | 0.0011 | 0.0013 | 0.0006 | 0.0019 | 0.0086 | 0.0004 | 0.0016 | 0.0006 | 0.0004 | 0.0005 | 0.0006 | 0.0156 | 0.0009 |
Bud Periphery | 0.0006 | 0.0003 | 0.001 | 0.0041 | 0.0061 | 0.0008 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0001 |
Bud Site | 0.0023 | 0.016 | 0.0021 | 0.0093 | 0.0043 | 0.0002 | 0.0038 | 0.0019 | 0.0032 | 0.0011 | 0.0001 | 0.0007 | 0.0008 | 0.0095 | 0.0017 | 0.0022 | 0.0012 | 0.0001 |
Cell Periphery | 0.0003 | 0.0007 | 0.0007 | 0.0011 | 0.0004 | 0.0003 | 0.0016 | 0.001 | 0.0013 | 0.0006 | 0.0004 | 0.0007 | 0.0009 | 0.0008 | 0.0009 | 0.0005 | 0.0003 | 0.0002 |
Cytoplasm | 0.1177 | 0.1904 | 0.184 | 0.2168 | 0.1602 | 0.1816 | 0.1597 | 0.1986 | 0.2929 | 0.2753 | 0.2639 | 0.2728 | 0.1528 | 0.3309 | 0.2736 | 0.1879 | 0.2793 | 0.2822 |
Cytoplasmic Foci | 0.0549 | 0.0577 | 0.0683 | 0.0657 | 0.0481 | 0.0638 | 0.0597 | 0.0846 | 0.0674 | 0.0446 | 0.0703 | 0.0415 | 0.0578 | 0.0505 | 0.0608 | 0.0436 | 0.0559 | 0.0474 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0018 | 0.0001 | 0.0004 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.2858 | 0.2485 | 0.282 | 0.2939 | 0.2364 | 0.3181 | 0.2958 | 0.3135 | 0.2576 | 0.2531 | 0.3472 | 0.3814 | 0.334 | 0.3337 | 0.2938 | 0.3759 | 0.2874 | 0.3754 |
Endosome | 0.2873 | 0.2627 | 0.2817 | 0.2181 | 0.3084 | 0.2602 | 0.1634 | 0.2189 | 0.1452 | 0.1373 | 0.1564 | 0.1165 | 0.1715 | 0.1398 | 0.1921 | 0.1814 | 0.1943 | 0.1438 |
Golgi | 0.0149 | 0.0222 | 0.028 | 0.0129 | 0.0172 | 0.0129 | 0.0239 | 0.0092 | 0.0145 | 0.0178 | 0.0156 | 0.0081 | 0.0155 | 0.0105 | 0.0122 | 0.0307 | 0.0143 | 0.0137 |
Lipid Particles | 0.112 | 0.0799 | 0.0455 | 0.0138 | 0.0413 | 0.0464 | 0.0896 | 0.0974 | 0.0729 | 0.04 | 0.0581 | 0.0629 | 0.1155 | 0.0528 | 0.0732 | 0.0589 | 0.019 | 0.0698 |
Mitochondria | 0.0062 | 0.0055 | 0.0058 | 0.01 | 0.006 | 0.0065 | 0.0128 | 0.0007 | 0.013 | 0.0055 | 0.0074 | 0.0074 | 0.0021 | 0.0009 | 0.0015 | 0.0048 | 0.002 | 0.0053 |
None | 0.0174 | 0.0207 | 0.0241 | 0.04 | 0.0491 | 0.0221 | 0.0932 | 0.0417 | 0.056 | 0.1202 | 0.0485 | 0.0627 | 0.0325 | 0.0119 | 0.0349 | 0.0228 | 0.0664 | 0.0223 |
Nuclear Periphery | 0.0571 | 0.0507 | 0.0363 | 0.0243 | 0.0426 | 0.0453 | 0.0284 | 0.0121 | 0.0137 | 0.023 | 0.0146 | 0.0124 | 0.042 | 0.0252 | 0.021 | 0.0165 | 0.0285 | 0.0186 |
Nucleolus | 0.0005 | 0.0003 | 0.0003 | 0.0043 | 0.0019 | 0.0007 | 0.0008 | 0.0001 | 0.0016 | 0.0036 | 0.0001 | 0.0009 | 0.0008 | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.0001 |
Nucleus | 0.005 | 0.0071 | 0.0042 | 0.0123 | 0.0133 | 0.0061 | 0.0039 | 0.0011 | 0.0037 | 0.0337 | 0.0015 | 0.0036 | 0.005 | 0.0032 | 0.0025 | 0.0028 | 0.0028 | 0.0031 |
Peroxisomes | 0.003 | 0.0018 | 0.0008 | 0.0008 | 0.0008 | 0.001 | 0.0132 | 0.0009 | 0.0106 | 0.0036 | 0.0018 | 0.0013 | 0.0034 | 0.0005 | 0.0014 | 0.0019 | 0.0019 | 0.0007 |
Punctate Nuclear | 0.0051 | 0.0034 | 0.0052 | 0.0403 | 0.0063 | 0.0098 | 0.0057 | 0.0018 | 0.0092 | 0.0121 | 0.003 | 0.0039 | 0.0098 | 0.0037 | 0.0053 | 0.0153 | 0.0038 | 0.0038 |
Vacuole | 0.0188 | 0.0202 | 0.0168 | 0.0158 | 0.0244 | 0.0148 | 0.0196 | 0.0137 | 0.0143 | 0.0103 | 0.0048 | 0.0147 | 0.0278 | 0.0204 | 0.0172 | 0.0134 | 0.0222 | 0.0091 |
Vacuole Periphery | 0.0052 | 0.0058 | 0.0042 | 0.0046 | 0.0077 | 0.0057 | 0.003 | 0.0017 | 0.0022 | 0.0023 | 0.0016 | 0.0021 | 0.0031 | 0.0019 | 0.0022 | 0.0033 | 0.0035 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.2778 | 31.2488 | 28.2288 | 26.7627 | 25.617 | 37.975 | 22.6665 | 28.2787 | 31.4584 | 27.9576 |
Translational Efficiency | 1.3048 | 0.8238 | 0.7206 | 0.9308 | 1.0337 | 0.669 | 1.3622 | 0.9657 | 0.9336 | 1.0762 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1181 | 1577 | 352 | 1514 | 1249 | 1363 | 73 | 609 | 2430 | 2940 | 425 | 2123 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 724.16 | 920.85 | 944.11 | 967.87 | 736.15 | 872.86 | 967.84 | 933.48 | 730.32 | 898.60 | 948.19 | 958.00 |
Standard Deviation | 87.81 | 122.26 | 99.34 | 131.58 | 101.45 | 105.87 | 96.20 | 160.60 | 95.25 | 117.42 | 99.21 | 141.38 |
Intensity Change Log 2 | — | 0.346658 | 0.382646 | 0.418505 | — | 0.245751 | 0.394769 | 0.342619 | — | 0.296672 | 0.388770 | 0.380749 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000687 | 0.001619 | 0.002299 | 0.006094 | 0.000531 | 0.000903 | 0.005754 | 0.009987 | 0.000607 | 0.001287 | 0.002892 | 0.007211 |
Bud Neck | 0.007321 | 0.003813 | 0.008846 | 0.010029 | 0.004217 | 0.004720 | 0.003177 | 0.009515 | 0.005725 | 0.004233 | 0.007872 | 0.009882 |
Bud Site | 0.005901 | 0.014877 | 0.023617 | 0.031978 | 0.003752 | 0.005463 | 0.013759 | 0.036493 | 0.004797 | 0.010513 | 0.021924 | 0.033273 |
Cell Periphery | 0.001152 | 0.000492 | 0.000368 | 0.000323 | 0.000460 | 0.000299 | 0.000591 | 0.000652 | 0.000796 | 0.000403 | 0.000406 | 0.000417 |
Cytoplasm | 0.203802* | 0.098948 | 0.190328* | 0.172476* | 0.178964* | 0.091914 | 0.224984* | 0.158096* | 0.191035* | 0.095687 | 0.196281* | 0.168351* |
Cytoplasmic Foci | 0.200878* | 0.247191* | 0.157874 | 0.184374* | 0.195102* | 0.250726* | 0.164173 | 0.222000* | 0.197909* | 0.248830* | 0.158956 | 0.195167* |
Eisosomes | 0.001103 | 0.000229 | 0.000164 | 0.000185 | 0.000465 | 0.000377 | 0.000303 | 0.000316 | 0.000775 | 0.000297 | 0.000188 | 0.000223 |
Endoplasmic Reticulum | 0.195694* | 0.210368* | 0.057151 | 0.040438 | 0.244658* | 0.120156* | 0.064576 | 0.091968 | 0.220861* | 0.168545* | 0.058427 | 0.055219 |
Endosome | 0.041591 | 0.094518 | 0.079424 | 0.097796 | 0.052615 | 0.094462 | 0.060501 | 0.116536 | 0.047257 | 0.094492 | 0.076174 | 0.103172 |
Golgi | 0.070580 | 0.093900 | 0.004413 | 0.013807 | 0.037408 | 0.097335 | 0.006610 | 0.025050 | 0.053529 | 0.095493 | 0.004790 | 0.017032 |
Lipid Particles | 0.150321* | 0.082703 | 0.063640 | 0.055939 | 0.141659* | 0.186162* | 0.072349 | 0.099526 | 0.145869* | 0.130667* | 0.065136 | 0.068442 |
Mitochondria | 0.040587 | 0.027302 | 0.000678 | 0.003563 | 0.015444 | 0.037577 | 0.000246 | 0.001746 | 0.027663 | 0.032066 | 0.000604 | 0.003042 |
Mitotic Spindle | 0.000730 | 0.005116 | 0.013768 | 0.050551 | 0.001116 | 0.006168 | 0.002381 | 0.016364 | 0.000929 | 0.005604 | 0.011812 | 0.040744 |
None | 0.002760 | 0.001979 | 0.000161 | 0.001640 | 0.003586 | 0.001274 | 0.000310 | 0.003570 | 0.003185 | 0.001652 | 0.000187 | 0.002194 |
Nuclear Periphery | 0.001795 | 0.002358 | 0.003314 | 0.002212 | 0.006508 | 0.002830 | 0.003012 | 0.001137 | 0.004217 | 0.002577 | 0.003262 | 0.001903 |
Nuclear Periphery Foci | 0.003678 | 0.011951 | 0.053554 | 0.022800 | 0.010003 | 0.007825 | 0.044596 | 0.030293 | 0.006929 | 0.010038 | 0.052015 | 0.024950 |
Nucleolus | 0.001314 | 0.000569 | 0.000500 | 0.000585 | 0.001774 | 0.000814 | 0.000507 | 0.000643 | 0.001550 | 0.000682 | 0.000501 | 0.000602 |
Nucleus | 0.018591 | 0.006005 | 0.036721 | 0.015362 | 0.024227 | 0.010221 | 0.043198 | 0.009514 | 0.021488 | 0.007960 | 0.037834 | 0.013684 |
Peroxisomes | 0.009603 | 0.003612 | 0.005976 | 0.008160 | 0.006631 | 0.007027 | 0.015440 | 0.010867 | 0.008075 | 0.005195 | 0.007601 | 0.008937 |
Vacuole | 0.031041 | 0.065831 | 0.295857* | 0.275831* | 0.060364 | 0.057553 | 0.272688* | 0.153293 | 0.046113 | 0.061993 | 0.291878* | 0.240680* |
Vacuole Periphery | 0.010873 | 0.026618 | 0.001347 | 0.005856 | 0.010517 | 0.016195 | 0.000844 | 0.002434 | 0.010690 | 0.021786 | 0.001260 | 0.004875 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.91 | -2.54 | -7.01 | -5.51 | -3.64 | -2.64 | -1.54 | -5.54 | -5.32 | -1.29 | -5.62 | -2.94 | -8.93 | -7.87 | -4.02 |
Bud Neck | 3.48 | -1.32 | -2.81 | -9.20 | -1.21 | -0.94 | 1.53 | -5.66 | -5.19 | -6.31 | 2.62 | -2.46 | -6.34 | -10.43 | -2.30 |
Bud Site | -6.37 | -5.03 | -12.31 | -7.12 | -2.01 | -2.04 | -1.88 | -8.89 | -8.32 | -3.79 | -6.75 | -5.65 | -15.67 | -11.76 | -3.27 |
Cell Periphery | 2.48 | 2.99 | 3.21 | 4.86 | 1.13 | 3.71 | -0.90 | -1.58 | -3.05 | -0.37 | 3.00 | 2.98 | 3.00 | 0.15 | -0.08 |
Cytoplasm | 15.50 | 0.83 | 2.79 | -12.14 | 0.91 | 13.80 | -1.43 | 1.56 | -7.23 | 1.94 | 20.59 | -0.58 | 2.43 | -14.66 | 1.85 |
Cytoplasmic Foci | -6.56 | 5.61 | 4.65 | 11.70 | -2.42 | -7.80 | 2.00 | -2.41 | 3.65 | -3.07 | -10.14 | 5.94 | 2.69 | 12.53 | -4.14 |
Eisosomes | 8.97 | 9.71 | 9.58 | 3.20 | -0.62 | 1.59 | 2.44 | 2.72 | 1.72 | -0.19 | 8.61 | 10.74 | 10.26 | 4.63 | -1.46 |
Endoplasmic Reticulum | -1.42 | 14.78 | 19.84 | 23.84 | 2.48 | 12.44 | 11.64 | 13.92 | 3.61 | -1.79 | 7.11 | 21.11 | 26.61 | 22.18 | 0.44 |
Endosome | -15.16 | -5.10 | -11.79 | 2.30 | -1.96 | -11.26 | -0.74 | -9.53 | -2.58 | -4.81 | -18.49 | -4.39 | -14.36 | 0.63 | -3.96 |
Golgi | -5.25 | 21.31 | 18.77 | 24.51 | -5.96 | -14.65 | 9.50 | 5.09 | 18.01 | -5.23 | -13.92 | 24.87 | 19.34 | 31.48 | -8.73 |
Lipid Particles | 11.15 | 12.86 | 17.75 | 7.67 | 1.08 | -6.01 | 4.70 | 6.06 | 11.06 | -1.71 | 3.16 | 14.29 | 19.38 | 16.16 | -0.85 |
Mitochondria | 4.30 | 15.72 | 14.33 | 12.86 | -3.14 | -8.54 | 11.95 | 10.23 | 15.62 | -3.29 | -2.19 | 19.02 | 16.65 | 19.62 | -3.72 |
Mitotic Spindle | -5.72 | -3.89 | -12.13 | -10.94 | -6.08 | -3.67 | -0.71 | -5.57 | -3.76 | -4.82 | -6.13 | -3.87 | -13.10 | -11.40 | -6.17 |
None | 1.28 | 5.52 | 1.70 | 0.73 | -1.76 | 2.27 | 3.12 | 0.10 | -1.22 | -1.71 | 2.53 | 5.31 | 1.40 | -0.42 | -2.50 |
Nuclear Periphery | -1.29 | -4.08 | -1.44 | 0.12 | 2.84 | 3.31 | 3.10 | 5.34 | 2.69 | 2.99 | 2.71 | 1.31 | 4.05 | 1.54 | 4.03 |
Nuclear Periphery Foci | -7.11 | -8.20 | -10.57 | -5.23 | 4.72 | 1.62 | -3.19 | -6.04 | -6.89 | 1.40 | -3.33 | -8.35 | -10.76 | -8.83 | 4.72 |
Nucleolus | 1.37 | 1.36 | 0.95 | -1.61 | -1.56 | 3.20 | 3.45 | 3.46 | 0.91 | -0.44 | 2.84 | 3.17 | 2.52 | -0.71 | -1.63 |
Nucleus | 8.09 | -4.39 | 1.07 | -8.37 | 4.89 | 9.03 | -1.89 | 8.00 | -0.36 | 3.26 | 12.37 | -4.42 | 4.86 | -7.04 | 5.96 |
Peroxisomes | 12.04 | 3.78 | 3.07 | -6.31 | -1.65 | -0.62 | -1.12 | -2.68 | -2.45 | 0.54 | 7.09 | 0.66 | -0.40 | -5.15 | -0.80 |
Vacuole | -10.60 | -21.71 | -39.93 | -33.46 | 0.70 | 0.71 | -8.20 | -12.55 | -12.86 | 4.39 | -6.22 | -22.31 | -38.64 | -35.31 | 3.60 |
Vacuole Periphery | -10.85 | 14.18 | 5.96 | 14.53 | -4.55 | -4.90 | 12.49 | 10.42 | 15.28 | -5.12 | -11.65 | 18.41 | 8.80 | 18.05 | -5.17 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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