Standard name
Human Ortholog
Description Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.73 0.84 0.75 0.87 0.83 0.83 0.69 0.65 0.68 0.71 0.68 0.67 0.88 0.79 0.71 – 0.81 0.78 0.22 0.35 0.25 0.19 0.14 0.15
Endoplasmic Reticulum 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0 – 0.14 0 0 0 0 0.05 0.05 0.06
Endosome 0.1 0 0.09 0.05 0 0 0 0 0 0 0 0 0 0.08 0.12 – 0.1 0 0.23 0.16 0.23 0.11 0.16 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.16 0.17 0.26 0.32 0.49 0.43 0.53 0.38 0.49 0.55 0 0 0 – 0 0 0.07 0.05 0.15 0.14 0.11 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.08 0.06 0 0.06 0 0.05 0 0 0.06 0 0 0.05 0.17 0.24 – 0.14 0.22 0.38 0.36 0.28 0.45 0.49 0.49
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 4 0 0 0 0 0 1 3 2 3 0 1 4 4 – 0 0 1 2 4 1 2 2
Bud 0 1 3 5 11 17 24 34 33 26 35 35 2 1 2 – 0 0 2 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 – 0 0 1 1 0 1 1 1
Bud Site 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 1 0 0 2 0 1 0 6 7 6 12 0 0 1 – 0 0 0 0 0 0 0 0
Cytoplasm 173 169 192 303 408 513 562 555 469 618 435 576 218 376 367 – 17 14 58 77 76 45 49 56
Endoplasmic Reticulum 15 13 6 1 1 2 4 9 1 7 8 8 15 13 3 – 3 0 8 4 10 11 16 21
Endosome 23 5 22 19 13 6 11 19 9 18 5 4 4 36 60 – 2 0 61 36 70 25 55 46
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 7 2 2 5 6 7
Mitochondria 5 0 41 60 126 195 401 371 362 328 313 477 0 1 0 – 0 0 20 10 48 34 39 39
Nucleus 1 0 1 0 0 7 7 16 4 3 3 0 4 2 1 – 0 0 0 1 0 1 0 1
Nuclear Periphery 1 0 0 0 1 1 10 3 1 1 1 1 0 0 0 – 0 0 1 0 3 0 1 1
Nucleolus 0 0 0 0 0 0 1 4 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1
Peroxisomes 0 0 1 1 1 0 0 1 1 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 2 2 1 3 2 0 7 2 2 0 2 5 – 0 0 2 0 3 0 2 1
Vac/Vac Membrane 36 16 16 8 31 23 37 35 29 50 25 23 12 82 126 – 3 4 102 80 86 107 172 181
Unique Cell Count 237 201 257 350 494 619 813 855 689 867 643 865 248 477 519 21 18 270 223 313 240 351 368
Labelled Cell Count 257 209 284 399 596 766 1061 1050 919 1068 836 1138 258 517 570 25 18 270 223 313 240 351 368


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.7 4.7 4.6 4.3 4.5 4.1 4.4 4.1 4.4 4.1 4.2 6.5 6.8 7.0 – 8.3 8.1 7.4 7.2 7.6
Std Deviation (1e-4) 0.9 1.0 0.8 1.2 1.2 1.2 1.2 1.2 1.0 1.2 1.1 0.9 0.8 1.1 1.0 – 1.6 1.4 1.6 1.2 1.4
Intensity Change (Log2) – – – -0.02 -0.12 -0.05 -0.19 -0.07 -0.19 -0.07 -0.17 -0.15 0.47 0.55 0.6 – 0.83 0.79 0.67 0.63 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm 3.7 2.6 2.8 -1.7 -2.9 -2.0 -1.1 -2.1 -2.5 3.8 1.3 -1.2 – 0.6 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 2.1 0.3 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 -3.5 -0.5 1.3 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.6 -8.8 -9.3 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole -2.5 0 -1.6 -1.1 -1.4 -1.3 -0.3 -1.5 -2.7 -0.7 4.2 6.1 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8683 1.2628 1.1446 0.3427 0.8781 1.1624 2.0795 2.9805 2.2617 2.1559 2.4278 2.3666 0.2675 0.5325 0.3617 0.5408 0.0787 0.3353
Actin 0.0208 0.0009 0.0114 0 0.0039 0.0003 0.0085 0.0002 0.0064 0.0001 0 0.0002 0.0012 0.0001 0.0001 0 0.0001 0.004
Bud 0.0014 0.0059 0.0236 0.0002 0.006 0.0008 0.0003 0.0006 0.0005 0.0021 0.0003 0.0002 0.0003 0.0002 0.0003 0.0003 0.0002 0.0005
Bud Neck 0.0011 0.0003 0.0019 0.0002 0.002 0.0018 0.0004 0.0002 0.0006 0.0003 0.0002 0.0013 0.0005 0.0002 0.0003 0.0005 0.0002 0.0009
Bud Periphery 0.0017 0.0015 0.0062 0.0001 0.0032 0.0009 0.0002 0.0002 0.0005 0.0008 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0 0.0005
Bud Site 0.0023 0.0209 0.0113 0.0002 0.0018 0.0021 0.0016 0.0011 0.0055 0.0014 0.0003 0.0002 0.0007 0.0005 0.0005 0.0003 0.0002 0.0004
Cell Periphery 0.0002 0.0007 0.0005 0 0.0001 0.0002 0.0005 0.0002 0.0003 0.0002 0 0.0001 0.0003 0.0001 0.0001 0 0 0
Cytoplasm 0.2227 0.4398 0.2567 0.2334 0.1551 0.3593 0.3204 0.3082 0.2678 0.2986 0.3105 0.3498 0.2661 0.3699 0.2868 0.2009 0.285 0.3012
Cytoplasmic Foci 0.119 0.0989 0.1163 0.1159 0.1595 0.1226 0.101 0.1102 0.0835 0.0959 0.0813 0.0999 0.1026 0.1125 0.0934 0.1179 0.0858 0.1252
Eisosomes 0.0004 0.0001 0.0001 0 0.0001 0 0.0003 0 0.0005 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0092 0.0043 0.0053 0.0032 0.0017 0.0025 0.0096 0.0059 0.0051 0.0026 0.0035 0.0051 0.0087 0.0045 0.0046 0.0024 0.0017 0.003
Endosome 0.2993 0.186 0.3146 0.5353 0.3135 0.3298 0.3042 0.2734 0.3772 0.3218 0.4754 0.3545 0.3911 0.3393 0.4318 0.5163 0.4985 0.4424
Golgi 0.0155 0.0062 0.022 0.0048 0.0218 0.0079 0.0236 0.0054 0.0226 0.0098 0.0055 0.0182 0.0124 0.0113 0.0113 0.0072 0.0057 0.0105
Lipid Particles 0.0094 0.0031 0.0058 0.0008 0.033 0.0082 0.0189 0.0054 0.0063 0.0023 0.0004 0.0011 0.0063 0.001 0.0025 0.0014 0.0004 0.0012
Mitochondria 0.0089 0.0019 0.0145 0.0019 0.0439 0.0072 0.0062 0.0013 0.0117 0.0034 0.0021 0.0075 0.0063 0.0068 0.0041 0.0017 0.0011 0.0074
None 0.2223 0.183 0.1283 0.0206 0.1751 0.0984 0.1163 0.2226 0.117 0.1404 0.0489 0.1058 0.1243 0.1074 0.0873 0.0329 0.0467 0.0604
Nuclear Periphery 0.0082 0.0023 0.0088 0.0015 0.0035 0.0022 0.0292 0.0021 0.003 0.0022 0.0018 0.0015 0.0052 0.0021 0.0024 0.0017 0.001 0.0014
Nucleolus 0.0007 0.0006 0.0009 0.0001 0.0006 0.0033 0.0004 0.0003 0.0005 0.0008 0.0001 0.0001 0.0005 0.0002 0.0002 0.0003 0 0.0002
Nucleus 0.004 0.0037 0.0058 0.0011 0.0022 0.0047 0.0063 0.0012 0.0016 0.0024 0.001 0.0013 0.0018 0.0026 0.0017 0.0016 0.0009 0.0012
Peroxisomes 0.007 0.0052 0.0125 0.0005 0.044 0.0026 0.0037 0.0007 0.0193 0.0008 0.0003 0.0027 0.0014 0.0011 0.0007 0.0007 0.0003 0.0016
Punctate Nuclear 0.0078 0.0017 0.0051 0.0005 0.0096 0.0029 0.0018 0.0009 0.0008 0.0006 0.0002 0.0006 0.0026 0.0009 0.0007 0.0022 0.0006 0.0011
Vacuole 0.0319 0.0279 0.0369 0.0598 0.0133 0.0329 0.0371 0.0525 0.0551 0.0923 0.0531 0.04 0.0551 0.0304 0.0548 0.0849 0.048 0.027
Vacuole Periphery 0.0061 0.0052 0.0114 0.0199 0.0059 0.0092 0.0096 0.0075 0.0142 0.021 0.0149 0.0099 0.0121 0.0087 0.0162 0.0266 0.0235 0.0097

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.8367 7.4725 6.9312 12.2506 15.5796 6.2659 13.4582 14.3422 13.8084 13.3324
Translational Efficiency 0.992 1.146 1.4525 0.8892 0.5823 1.8727 0.7945 0.9846 1.1448 0.7822

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
Localization
Cell Percentages cytoplasm (30%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-3

Rav2

Rav2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rav2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available