Standard name | |
---|---|
Human Ortholog | |
Description | Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 26 | 32 | 62 | 41 | 60 | 34 | 60 | 39 | 9 | 14 | 14 | 38 | 10 | 12 | 13 | 16 | 16 | 9 | 9 | 22 | 28 | 9 | 44 | 25 |
Bud | 2 | 10 | 9 | 9 | 11 | 23 | 31 | 23 | 35 | 34 | 39 | 29 | 1 | 3 | 2 | 0 | 4 | 0 | 0 | 1 | 2 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 4 | 0 | 3 | 2 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 9 | 2 | 3 | 2 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 24 | 18 | 11 | 16 | 16 | 18 | 13 | 13 | 10 | 14 | 8 | 22 | 28 | 22 | 15 | 9 | 0 | 2 | 12 | 7 | 28 | 23 |
Cytoplasm | 55 | 93 | 161 | 178 | 216 | 248 | 302 | 267 | 272 | 227 | 188 | 219 | 37 | 71 | 81 | 23 | 13 | 15 | 9 | 21 | 27 | 6 | 3 | 3 |
Endoplasmic Reticulum | 200 | 129 | 129 | 82 | 110 | 107 | 199 | 76 | 46 | 41 | 10 | 78 | 204 | 144 | 163 | 5 | 6 | 2 | 262 | 207 | 379 | 128 | 227 | 190 |
Endosome | 0 | 10 | 1 | 11 | 9 | 12 | 4 | 1 | 16 | 6 | 3 | 4 | 5 | 18 | 11 | 2 | 1 | 2 | 0 | 6 | 3 | 0 | 0 | 2 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 3 | 2 | 2 | 1 | 0 | 1 |
Mitochondria | 21 | 45 | 1 | 31 | 28 | 98 | 160 | 117 | 213 | 222 | 239 | 128 | 21 | 11 | 9 | 3 | 5 | 2 | 11 | 53 | 90 | 19 | 22 | 21 |
Nucleus | 1 | 0 | 1 | 0 | 2 | 0 | 2 | 9 | 3 | 7 | 9 | 4 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 3 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 0 | 6 | 4 | 0 | 9 | 0 | 3 | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 6 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 4 | 3 | 7 | 5 | 9 | 8 | 5 | 10 | 1 | 13 | 0 | 4 | 0 | 3 | 4 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 14 | 18 | 17 | 34 | 32 | 38 | 64 | 23 | 29 | 44 | 24 | 4 | 10 | 7 | 6 | 2 | 2 | 7 | 16 | 23 | 2 | 3 | 5 |
Unique Cell Count | 269 | 280 | 376 | 346 | 438 | 507 | 697 | 552 | 545 | 495 | 463 | 475 | 251 | 270 | 275 | 67 | 52 | 38 | 311 | 350 | 596 | 182 | 340 | 283 |
Labelled Cell Count | 315 | 336 | 415 | 390 | 492 | 578 | 825 | 630 | 639 | 618 | 568 | 553 | 300 | 303 | 318 | 83 | 68 | 45 | 311 | 350 | 596 | 182 | 340 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 5.4 | 6.2 | 5.9 | 6.3 | 5.1 | 5.5 | 5.7 | 4.8 | 4.7 | 4.4 | 5.3 | 6.2 | 6.6 | 6.5 | 9.4 | 8.7 | 9.6 | 5.7 | 5.8 | 6.4 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.7 | 0.7 | 1.0 | 0.7 | 0.9 | 1.0 | 0.8 | 1.0 | 0.7 | 0.9 | 0.8 | 1.2 | 1.0 | 1.5 | 1.6 | 2.3 | 1.0 | 1.0 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.02 | -0.28 | -0.19 | -0.13 | -0.38 | -0.4 | -0.51 | -0.24 | -0.01 | 0.07 | 0.06 | 0.6 | 0.48 | 0.62 | -0.14 | -0.1 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.8 | -1.1 | -4.6 | -3.9 | -4.5 | 0 | 0 | 0 | -3.8 | -4.8 | -4.7 | -4.6 | 1.5 | 2.5 | 1.1 |
Bud | 0.2 | 0.1 | 1.7 | 1.7 | 1.5 | 0 | 0 | 0 | 2.6 | 0 | -1.2 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.7 | -2.7 | -2.3 | -3.4 | -2.2 | 0 | 0 | 0 | -2.4 | -1.8 | 0.9 | 1.8 | 6.5 | 0 | 0 |
Cytoplasm | 2.3 | 1.9 | 1.8 | 0.2 | 1.7 | 2.1 | 0.9 | -0.6 | 1.0 | -7.4 | -4.3 | -3.5 | -1.3 | -2.5 | -0.4 |
Endoplasmic Reticulum | -3.1 | -2.9 | -4.4 | -2.0 | -7.4 | 0 | 0 | 0 | -6.0 | 11.5 | 4.8 | 6.3 | -4.4 | -3.3 | -3.7 |
Endosome | 3.1 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.7 | 3.5 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.7 | 4.7 | 8.9 | 9.9 | 9.4 | 0 | 0 | 0 | 10.8 | 5.4 | 3.5 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 1.7 | 1.0 | 0.5 | 3.6 | -0.4 | 0.7 | 2.6 | 0.2 | -2.1 | -0.7 | -1.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9295 | 1.1115 | 1.0706 | 1.2395 | 0.6343 | 1.0444 | 1.6739 | 1.9906 | 1.8361 | 1.8883 | 1.8875 | 1.771 | 1.8219 | 2.1146 | 1.8536 | 1.7866 | 1.6364 | 1.7392 |
Actin | 0.0115 | 0.0091 | 0.0119 | 0.0315 | 0.0072 | 0.0088 | 0.0368 | 0.0141 | 0.0381 | 0.0287 | 0.0063 | 0.0168 | 0.0436 | 0.005 | 0.0029 | 0.0436 | 0.0066 | 0.0148 |
Bud | 0.0011 | 0.001 | 0.0005 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0005 | 0.0012 | 0.0008 | 0.0015 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Bud Neck | 0.0024 | 0.0026 | 0.0097 | 0.0023 | 0.0019 | 0.007 | 0.0011 | 0.0015 | 0.0052 | 0.007 | 0.0021 | 0.0092 | 0.0019 | 0.0009 | 0.004 | 0.0148 | 0.0028 | 0.0059 |
Bud Periphery | 0.0026 | 0.0029 | 0.0014 | 0.0007 | 0.0013 | 0.0009 | 0.0011 | 0.0012 | 0.0039 | 0.0016 | 0.0085 | 0.0011 | 0.0017 | 0.0003 | 0.0003 | 0.001 | 0.0002 | 0.0002 |
Bud Site | 0.0052 | 0.0252 | 0.0183 | 0.0009 | 0.0014 | 0.0006 | 0.0033 | 0.0273 | 0.0094 | 0.0021 | 0.0014 | 0.0008 | 0.0038 | 0.0061 | 0.01 | 0.0023 | 0.0004 | 0.0003 |
Cell Periphery | 0.0077 | 0.0082 | 0.0083 | 0.0011 | 0.002 | 0.0038 | 0.0064 | 0.0149 | 0.0145 | 0.0052 | 0.0046 | 0.0046 | 0.0112 | 0.0102 | 0.0058 | 0.0121 | 0.0016 | 0.0018 |
Cytoplasm | 0.1859 | 0.2947 | 0.2514 | 0.3202 | 0.2855 | 0.3151 | 0.1499 | 0.2087 | 0.1948 | 0.2111 | 0.1367 | 0.2441 | 0.1495 | 0.2532 | 0.2066 | 0.1766 | 0.1678 | 0.2222 |
Cytoplasmic Foci | 0.0216 | 0.0246 | 0.0275 | 0.0269 | 0.0202 | 0.037 | 0.0405 | 0.0245 | 0.0269 | 0.0395 | 0.0442 | 0.026 | 0.0201 | 0.018 | 0.0288 | 0.0166 | 0.0183 | 0.0179 |
Eisosomes | 0.0006 | 0.0006 | 0.0006 | 0.0004 | 0.001 | 0.0006 | 0.0007 | 0.0012 | 0.0012 | 0.0007 | 0.0002 | 0.0003 | 0.0013 | 0.0004 | 0.0003 | 0.0007 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.5717 | 0.4114 | 0.4768 | 0.3467 | 0.385 | 0.4194 | 0.5167 | 0.5036 | 0.5032 | 0.4013 | 0.4361 | 0.54 | 0.5793 | 0.5441 | 0.5956 | 0.6034 | 0.6786 | 0.6163 |
Endosome | 0.0498 | 0.0595 | 0.0529 | 0.0408 | 0.0736 | 0.0431 | 0.0485 | 0.0474 | 0.0575 | 0.0474 | 0.1581 | 0.046 | 0.0331 | 0.0445 | 0.0376 | 0.0262 | 0.0126 | 0.0192 |
Golgi | 0.0175 | 0.01 | 0.0101 | 0.0071 | 0.0084 | 0.0087 | 0.0204 | 0.0243 | 0.0244 | 0.0408 | 0.0353 | 0.0127 | 0.011 | 0.0067 | 0.007 | 0.0128 | 0.0039 | 0.009 |
Lipid Particles | 0.0368 | 0.0354 | 0.034 | 0.0167 | 0.0206 | 0.0287 | 0.0671 | 0.0594 | 0.0458 | 0.0868 | 0.0606 | 0.0263 | 0.0563 | 0.043 | 0.0452 | 0.0114 | 0.0215 | 0.0212 |
Mitochondria | 0.0057 | 0.0044 | 0.0048 | 0.0091 | 0.0139 | 0.0141 | 0.0057 | 0.0069 | 0.0262 | 0.0156 | 0.0662 | 0.0063 | 0.0023 | 0.0009 | 0.0013 | 0.002 | 0.0037 | 0.0019 |
None | 0.0462 | 0.0687 | 0.0583 | 0.1222 | 0.1404 | 0.0847 | 0.0462 | 0.0382 | 0.0249 | 0.0735 | 0.0041 | 0.0412 | 0.0477 | 0.0454 | 0.0347 | 0.0477 | 0.0047 | 0.055 |
Nuclear Periphery | 0.0146 | 0.0209 | 0.0126 | 0.0382 | 0.0171 | 0.0098 | 0.0308 | 0.0117 | 0.0089 | 0.0068 | 0.0083 | 0.0118 | 0.0151 | 0.0087 | 0.0083 | 0.0106 | 0.0068 | 0.0056 |
Nucleolus | 0.0006 | 0.0005 | 0.0005 | 0.0007 | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0207 | 0.0001 |
Nucleus | 0.0022 | 0.0036 | 0.0039 | 0.015 | 0.0046 | 0.0048 | 0.003 | 0.0017 | 0.0014 | 0.0016 | 0.0018 | 0.0017 | 0.0042 | 0.0014 | 0.0013 | 0.0015 | 0.0114 | 0.0011 |
Peroxisomes | 0.0045 | 0.0007 | 0.0025 | 0.0026 | 0.0009 | 0.002 | 0.008 | 0.0008 | 0.0014 | 0.0164 | 0.0031 | 0.0008 | 0.0027 | 0.0003 | 0.0008 | 0.0007 | 0.0267 | 0.0015 |
Punctate Nuclear | 0.0013 | 0.0019 | 0.0024 | 0.0076 | 0.0022 | 0.0023 | 0.0022 | 0.0013 | 0.0012 | 0.002 | 0.0023 | 0.0011 | 0.0054 | 0.0009 | 0.0013 | 0.0006 | 0.0065 | 0.0007 |
Vacuole | 0.0088 | 0.0122 | 0.01 | 0.0075 | 0.0094 | 0.0067 | 0.0085 | 0.0094 | 0.0085 | 0.0091 | 0.0144 | 0.007 | 0.0067 | 0.0092 | 0.0071 | 0.0131 | 0.004 | 0.0044 |
Vacuole Periphery | 0.0016 | 0.0018 | 0.0015 | 0.0015 | 0.0023 | 0.0013 | 0.0024 | 0.0012 | 0.0014 | 0.0015 | 0.0037 | 0.0016 | 0.0015 | 0.0007 | 0.0007 | 0.0018 | 0.0011 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.0036 | 20.111 | 18.7408 | 16.8039 | 13.5667 | 12.5496 | 26.0089 | 21.0651 | 19.7425 | 23.8099 |
Translational Efficiency | 0.7214 | 0.8282 | 0.7008 | 0.7956 | 0.7837 | 1.0743 | 0.6892 | 0.698 | 0.7202 | 0.6252 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
940 | 1018 | 1351 | 1094 | 2118 | 1251 | 208 | 154 | 3058 | 2269 | 1559 | 1248 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 725.98 | 866.82 | 1123.37 | 919.77 | 843.25 | 810.17 | 1059.73 | 981.66 | 807.20 | 835.59 | 1114.88 | 927.41 |
Standard Deviation | 367.24 | 119.00 | 158.38 | 114.08 | 103.86 | 114.27 | 102.14 | 131.32 | 227.72 | 119.78 | 153.61 | 118.12 |
Intensity Change Log 2 | — | 0.255803 | 0.629831 | 0.341343 | — | -0.057736 | 0.329664 | 0.219263 | — | 0.095817 | 0.476321 | 0.277028 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000728 | 0.001800 | 0.000939 | 0.003279 | 0.001297 | 0.000718 | 0.000860 | 0.005783 | 0.001122 | 0.001204 | 0.000928 | 0.003588 |
Bud Neck | 0.005021 | 0.001487 | 0.002194 | 0.007850 | 0.002212 | 0.003528 | 0.001910 | 0.005078 | 0.003075 | 0.002613 | 0.002156 | 0.007508 |
Bud Site | 0.002905 | 0.005013 | 0.005138 | 0.018301 | 0.002816 | 0.006150 | 0.002390 | 0.006818 | 0.002843 | 0.005640 | 0.004771 | 0.016884 |
Cell Periphery | 0.001538 | 0.002750 | 0.000303 | 0.000752 | 0.002417 | 0.001472 | 0.000628 | 0.000520 | 0.002147 | 0.002045 | 0.000346 | 0.000724 |
Cytoplasm | 0.244029* | 0.134059* | 0.091798 | 0.207446* | 0.206260* | 0.137311* | 0.092070 | 0.196895* | 0.217870* | 0.135852* | 0.091834 | 0.206144* |
Cytoplasmic Foci | 0.087863 | 0.032766 | 0.007615 | 0.051192 | 0.027284 | 0.053691 | 0.031081 | 0.068971 | 0.045905 | 0.044303 | 0.010746 | 0.053386 |
Eisosomes | 0.001550 | 0.001577 | 0.000029 | 0.000437 | 0.002593 | 0.000886 | 0.000232 | 0.000488 | 0.002272 | 0.001196 | 0.000056 | 0.000443 |
Endoplasmic Reticulum | 0.352929* | 0.670818* | 0.527693* | 0.308360* | 0.611017* | 0.569220* | 0.393370* | 0.129041* | 0.531683* | 0.614803* | 0.509772* | 0.286232* |
Endosome | 0.011893 | 0.008544 | 0.012261 | 0.019268 | 0.005077 | 0.017257 | 0.019429 | 0.024400 | 0.007172 | 0.013348 | 0.013218 | 0.019902 |
Golgi | 0.029360 | 0.039363 | 0.000831 | 0.003452 | 0.035850 | 0.033885 | 0.001248 | 0.011853 | 0.033855 | 0.036343 | 0.000887 | 0.004489 |
Lipid Particles | 0.174460* | 0.062737 | 0.008705 | 0.046070 | 0.052947 | 0.091216 | 0.045902 | 0.039176 | 0.090299 | 0.078439 | 0.013668 | 0.045220 |
Mitochondria | 0.012098 | 0.007172 | 0.000568 | 0.001870 | 0.005656 | 0.013613 | 0.000276 | 0.004709 | 0.007636 | 0.010723 | 0.000529 | 0.002220 |
Mitotic Spindle | 0.000330 | 0.000163 | 0.003108 | 0.016232 | 0.000621 | 0.000668 | 0.000946 | 0.021120 | 0.000531 | 0.000441 | 0.002820 | 0.016836 |
None | 0.002728 | 0.002689 | 0.000397 | 0.001186 | 0.010475 | 0.002350 | 0.000300 | 0.000692 | 0.008094 | 0.002502 | 0.000384 | 0.001126 |
Nuclear Periphery | 0.007557 | 0.001100 | 0.123958* | 0.022573 | 0.001099 | 0.008780 | 0.064559 | 0.031198 | 0.003084 | 0.005334 | 0.116033* | 0.023637 |
Nuclear Periphery Foci | 0.006683 | 0.005999 | 0.166966 | 0.086117 | 0.003818 | 0.008967 | 0.182159 | 0.098081 | 0.004699 | 0.007635 | 0.168993 | 0.087593 |
Nucleolus | 0.000434 | 0.000105 | 0.000082 | 0.000458 | 0.000141 | 0.000786 | 0.000442 | 0.000388 | 0.000231 | 0.000480 | 0.000130 | 0.000449 |
Nucleus | 0.027769 | 0.005583 | 0.022638 | 0.064375 | 0.012725 | 0.012356 | 0.044362 | 0.072774 | 0.017349 | 0.009317 | 0.025536 | 0.065412 |
Peroxisomes | 0.010280 | 0.007157 | 0.000227 | 0.002010 | 0.007473 | 0.007307 | 0.002206 | 0.004529 | 0.008336 | 0.007240 | 0.000491 | 0.002321 |
Vacuole | 0.017006 | 0.007444 | 0.023956 | 0.136228 | 0.007340 | 0.025092 | 0.115263 | 0.274810* | 0.010311 | 0.017174 | 0.036138 | 0.153329 |
Vacuole Periphery | 0.002841 | 0.001676 | 0.000594 | 0.002541 | 0.000883 | 0.004745 | 0.000369 | 0.002676 | 0.001485 | 0.003368 | 0.000564 | 0.002558 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.74 | -0.88 | -3.44 | -1.90 | -3.01 | 2.46 | 1.34 | -1.04 | -1.24 | -1.17 | -0.35 | 0.45 | -3.11 | -3.01 | -3.22 |
Bud Neck | 4.24 | 2.94 | -3.20 | -7.87 | -6.64 | -2.00 | 0.25 | -3.90 | -2.37 | -3.84 | 0.99 | 1.50 | -6.38 | -6.79 | -7.18 |
Bud Site | -1.41 | -2.01 | -6.54 | -5.25 | -5.25 | -2.25 | 0.10 | -3.31 | -1.38 | -3.26 | -2.68 | -2.33 | -7.15 | -5.35 | -5.60 |
Cell Periphery | -2.18 | 5.43 | 3.54 | 3.93 | -6.18 | 3.37 | 7.88 | 9.19 | 4.37 | 0.91 | 0.35 | 11.60 | 9.16 | 5.14 | -5.58 |
Cytoplasm | 12.08 | 17.37 | 2.97 | -8.82 | -14.01 | 10.15 | 8.91 | 0.50 | -3.16 | -4.69 | 15.39 | 21.70 | 0.83 | -9.84 | -14.78 |
Cytoplasmic Foci | 11.26 | 18.48 | 7.21 | -5.48 | -16.17 | -7.98 | -1.50 | -4.95 | -1.17 | -3.57 | 0.59 | 17.29 | -2.63 | -2.96 | -16.11 |
Eisosomes | -0.09 | 6.27 | 4.58 | 5.89 | -8.24 | 6.34 | 9.24 | 7.97 | 3.35 | -1.07 | 5.01 | 11.66 | 9.54 | 7.11 | -8.04 |
Endoplasmic Reticulum | -21.07* | -12.28 | 5.76 | 28.45* | 19.92 | 3.37 | 9.92 | 36.88* | 29.81* | 10.77 | -8.43 | 2.95 | 26.28 | 31.91 | 21.63 |
Endosome | 3.44 | -0.70 | -5.41 | -8.31 | -5.21 | -9.64 | -8.15 | -5.76 | -1.04 | -0.17 | -7.69 | -10.04 | -11.67 | -4.57 | -4.86 |
Golgi | -3.29 | 15.51 | 13.73 | 14.66 | -3.67 | 0.78 | 22.79 | 5.19 | 4.66 | -1.12 | -1.25 | 27.98 | 21.20 | 18.38 | -3.14 |
Lipid Particles | 15.16 | 25.03 | 18.91 | 4.74 | -15.02 | -8.49 | 1.28 | 5.05 | 10.82 | 2.13 | 3.13 | 26.71 | 14.56 | 10.84 | -12.72 |
Mitochondria | 2.21 | 6.03 | 5.33 | 4.57 | -3.26 | -5.09 | 12.68 | 1.02 | 3.63 | -1.14 | -2.64 | 10.77 | 7.26 | 8.25 | -3.14 |
Mitotic Spindle | 1.03 | -3.89 | -6.11 | -6.18 | -4.85 | -0.10 | -0.60 | -2.50 | -2.50 | -2.44 | 0.25 | -3.44 | -6.46 | -6.51 | -5.43 |
None | 0.04 | 4.06 | 2.20 | 1.89 | -1.91 | 5.57 | 7.34 | 6.44 | 1.83 | -2.11 | 5.31 | 7.92 | 6.66 | 2.45 | -2.20 |
Nuclear Periphery | 6.00 | -22.27 | -6.30 | -10.84 | 18.96 | -6.02 | -8.25 | -4.92 | -2.95 | 4.71 | -2.85 | -24.57 | -10.44 | -8.60 | 19.42 |
Nuclear Periphery Foci | 0.80 | -30.97* | -19.12 | -19.24 | 13.25 | -5.40 | -12.41 | -7.20 | -6.66 | 5.87 | -4.60 | -33.83 | -21.02 | -20.01 | 14.48 |
Nucleolus | 4.39 | 4.74 | -1.28 | -4.11 | -4.29 | -2.40 | -2.02 | -3.57 | 1.21 | 0.20 | -1.70 | 2.50 | -3.31 | -0.52 | -4.09 |
Nucleus | 12.90 | 1.39 | -11.56 | -19.93 | -12.32 | 0.11 | -4.99 | -6.25 | -6.24 | -2.20 | 8.30 | -5.26 | -16.89 | -19.57 | -11.96 |
Peroxisomes | 2.65 | 10.06 | 8.08 | 7.23 | -8.00 | 0.18 | 2.67 | 2.28 | 2.17 | -0.15 | 1.71 | 15.02 | 11.23 | 9.41 | -4.27 |
Vacuole | 6.88 | -4.83 | -23.10 | -24.67 | -21.23 | -8.39 | -9.41 | -14.82 | -14.02 | -8.20 | -5.26 | -12.01 | -27.37 | -26.03 | -21.40 |
Vacuole Periphery | 2.06 | 4.22 | 0.81 | -0.82 | -2.60 | -6.41 | 5.05 | -0.59 | 2.06 | -0.97 | -4.89 | 4.73 | -1.13 | 1.90 | -2.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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