Standard name
Human Ortholog
Description RNA binding protein; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.07 0 0.09 0 0 0.07 0 – – – 0.06 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.06 0 0.15 0 0.07 0.09 0.07 0.36 0.22 0.42 0.17 – – – 0.05 0 0 0 0 0 0 0 0
Nucleus 0.97 0.93 0.89 0.92 0.94 0.88 0.94 0.84 0.86 0.86 0.83 0.91 – – – 0.83 0.89 0.85 0.95 0.87 0.76 0.69 0.78 0.85
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.08 0 0 0 0 0.05 0.06 0.05 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.06 0 0
Vac/Vac Membrane 0 0 0.07 0 0 0 0 0 0 0 0 0 – – – 0.07 0.06 0.07 0 0.07 0.08 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 1 0 1 – – – 1 0 0 0 2 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 3 5 1 3 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
Cytoplasm 1 7 1 0 8 10 9 23 4 6 12 5 – – – 20 9 12 0 5 1 0 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 2 0 0 0 0 – – – 3 4 4 0 1 0 0 0 0
Endosome 0 0 0 0 0 1 0 1 0 0 0 0 – – – 3 2 3 0 4 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 1 0 0 1 2 0 0 0
Mitochondria 21 21 0 4 0 10 19 18 40 31 70 29 – – – 15 5 5 0 3 1 0 0 0
Nucleus 131 315 25 24 168 134 194 223 96 121 137 157 – – – 277 193 219 123 312 52 13 17 45
Nuclear Periphery 0 0 0 1 0 1 0 2 1 1 1 4 – – – 0 2 0 0 2 0 0 0 0
Nucleolus 1 0 0 2 0 1 1 6 5 8 8 5 – – – 6 2 5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 2 0 0 1 1 1 0 0 – – – 2 0 4 1 1 1 1 0 1
Vac/Vac Membrane 1 15 2 0 2 3 3 2 1 0 2 2 – – – 22 12 18 3 23 5 0 0 3
Unique Cell Count 135 338 28 26 178 152 207 264 111 140 166 172 332 217 258 131 360 69 20 22 54
Labelled Cell Count 155 360 28 31 180 160 227 279 151 174 231 206 351 230 270 131 360 69 20 22 54


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.7 6.9 5.5 6.9 5.8 5.7 6.3 4.4 5.0 4.7 5.5 – – – 8.8 8.6 8.1 – – 5.6
Std Deviation (1e-4) 0.8 1.0 1.2 1.4 2.2 1.8 1.7 2.4 1.3 1.1 1.6 2.4 – – – 2.4 2.1 2.2 – – 0.9
Intensity Change (Log2) – – – -0.34 -0.0 -0.24 -0.27 -0.13 -0.64 -0.45 -0.55 -0.33 – – – 0.35 0.32 0.24 – – -0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 0 2.8 0 0 – – – 0 0 0
Nucleus 0.4 1.0 -0.2 0.9 -0.7 -0.4 -0.4 -0.9 0.3 – – – -0.8 -0.1 -0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0062 1.8777 1.8599 1.4151 1.5441 1.7172 0.7972 1.4757 1.2734 1.1726 1.605 1.3193 1.0238 1.312 0.6662 0.7063 0.6119 0.8986
Actin 0.0084 0 0.0014 0.0002 0.0039 0.002 0.032 0 0.0049 0.0247 0.0206 0.0043 0.0826 0 0.016 0 0.0101 0.0074
Bud 0.0012 0.0002 0.0002 0.0001 0.0001 0.0002 0.0004 0 0.0006 0.0006 0.0005 0.0002 0.0005 0 0.0003 0 0.0001 0
Bud Neck 0.0019 0.0001 0.0005 0.0001 0.0003 0.0007 0.0039 0 0.0011 0.0019 0.001 0.0011 0.0018 0 0.0004 0 0.0007 0.0006
Bud Periphery 0.0018 0 0.0004 0.0002 0.0002 0.0005 0.0005 0 0.0014 0.0007 0.0008 0.0006 0.0008 0.0001 0.0007 0 0.0003 0.0001
Bud Site 0.002 0.0001 0.0007 0.0002 0.0004 0.0005 0.0097 0 0.0022 0.0077 0.0046 0.0025 0.0016 0 0.0007 0 0.0004 0.0001
Cell Periphery 0.0005 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0003 0.0005 0.0003 0.0002 0.0002 0 0.0002 0 0.0001 0
Cytoplasm 0.0016 0.0004 0.0007 0.0002 0.001 0.0003 0.0093 0.0001 0.0014 0.0019 0.0012 0.002 0.0039 0.0005 0.0006 0.0019 0.0016 0.0006
Cytoplasmic Foci 0.0048 0.0001 0.0039 0.0011 0.0226 0.0007 0.012 0 0.0047 0.0091 0.0158 0.0064 0.0067 0 0.0007 0 0.0115 0.001
Eisosomes 0.0002 0 0.0001 0 0 0 0.0003 0 0.0001 0.0002 0.0006 0 0.0005 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0033 0 0.0009 0.0004 0.0008 0.0007 0.0023 0 0.0006 0.0008 0.0006 0.0026 0.0049 0 0.0007 0 0.0034 0.0018
Endosome 0.0177 0 0.0053 0.0028 0.0126 0.0026 0.0096 0 0.0079 0.0077 0.0537 0.0106 0.0198 0 0.0057 0 0.0206 0.0032
Golgi 0.0039 0 0.0016 0.0005 0.0031 0.0019 0.0043 0 0.0036 0.0045 0.0179 0.0023 0.0077 0 0.0012 0 0.0066 0.0012
Lipid Particles 0.0181 0 0.0072 0.0135 0.0087 0.0048 0.0106 0 0.0014 0.0176 0.0716 0.0043 0.0087 0 0.0012 0 0.0203 0.001
Mitochondria 0.0222 0.0001 0.0052 0.0039 0.002 0.0065 0.0049 0.0001 0.0162 0.0054 0.0331 0.0025 0.0055 0.0002 0.0021 0.0001 0.0047 0.0003
None 0.0023 0.0001 0.0009 0.0004 0.0016 0.0001 0.0035 0 0.0003 0.0121 0.0004 0.0004 0.0037 0.0001 0.0005 0.0208 0.0043 0.0005
Nuclear Periphery 0.0305 0.0036 0.006 0.0107 0.0434 0.006 0.004 0.0015 0.0046 0.004 0.0027 0.0081 0.0251 0.0015 0.0021 0.0004 0.0243 0.01
Nucleolus 0.0136 0.0087 0.009 0.0089 0.0174 0.0243 0.0073 0.0045 0.005 0.0134 0.0338 0.0149 0.0051 0.0052 0.0085 0.0022 0.0157 0.0093
Nucleus 0.8264 0.9798 0.9359 0.9468 0.7809 0.9386 0.8678 0.9932 0.9342 0.8644 0.7109 0.9298 0.7839 0.9892 0.9551 0.9728 0.8272 0.9566
Peroxisomes 0.011 0 0.0022 0.0011 0.0125 0.0012 0.0087 0 0.0026 0.0171 0.0142 0.0009 0.0028 0 0.0004 0 0.0387 0.0012
Punctate Nuclear 0.0168 0.0066 0.0152 0.0059 0.0867 0.0075 0.0066 0.0004 0.0022 0.0023 0.0079 0.0039 0.0307 0.0028 0.0005 0.0017 0.0067 0.0044
Vacuole 0.0067 0.0002 0.0015 0.0013 0.0004 0.0005 0.0016 0.0001 0.004 0.0031 0.0061 0.0018 0.0017 0.0002 0.0017 0 0.0008 0.0002
Vacuole Periphery 0.0052 0.0001 0.0012 0.0015 0.0011 0.0003 0.0005 0 0.0008 0.0006 0.0019 0.0006 0.0018 0.0001 0.0009 0 0.0015 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.679 7.8151 7.456 8.7799 6.0722 14.9785 11.0061 11.0473 7.258 11.6528
Translational Efficiency 1.45 1.5927 1.7513 1.0617 1.701 0.7194 1.0848 0.9212 1.2137 1.0004

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
807 1087 151 1539 1338 1103 1205 163 2145 2190 1356 1702

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 657.34 589.11 1006.48 941.11 765.21 761.93 962.36 1016.52 724.63 676.15 967.27 948.33
Standard Deviation 77.30 109.42 109.93 120.17 89.26 101.03 109.71 131.62 99.74 136.19 110.61 123.33
Intensity Change Log 2 — -0.158103 0.614607 0.517724 — -0.006197 0.330721 0.409711 — -0.074409 0.468867 0.460629

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000050 0.000432 0.001549 0.000942 0.000131 0.000178 0.000448 0.001354 0.000101 0.000304 0.000571 0.000982
Bud Neck 0.000704 0.004919 0.006401 0.018615 0.001208 0.002964 0.008429 0.010948 0.001019 0.003934 0.008203 0.017881
Bud Site 0.000218 0.003519 0.001519 0.006543 0.000414 0.000964 0.000878 0.010866 0.000340 0.002232 0.000950 0.006957
Cell Periphery 0.000065 0.000586 0.000108 0.000131 0.000075 0.000151 0.000100 0.000094 0.000072 0.000367 0.000101 0.000127
Cytoplasm 0.026154 0.048155 0.006927 0.008250 0.007363 0.014677 0.005261 0.009873 0.014432 0.031294 0.005447 0.008406
Cytoplasmic Foci 0.002479 0.015820 0.000256 0.003034 0.001561 0.003869 0.000103 0.000191 0.001907 0.009801 0.000120 0.002762
Eisosomes 0.000011 0.000054 0.000051 0.000024 0.000012 0.000017 0.000023 0.000023 0.000012 0.000035 0.000026 0.000024
Endoplasmic Reticulum 0.000623 0.004747 0.012391 0.008553 0.002200 0.003506 0.005350 0.006011 0.001607 0.004122 0.006134 0.008310
Endosome 0.000161 0.001570 0.000604 0.002308 0.000338 0.002303 0.000281 0.000507 0.000272 0.001939 0.000317 0.002135
Golgi 0.000108 0.001276 0.000119 0.001392 0.000325 0.000855 0.000053 0.000075 0.000243 0.001064 0.000061 0.001266
Lipid Particles 0.000362 0.002600 0.000601 0.000263 0.000461 0.001556 0.000125 0.000188 0.000423 0.002074 0.000178 0.000256
Mitochondria 0.002266 0.003415 0.000966 0.003854 0.001161 0.002842 0.000702 0.001360 0.001577 0.003126 0.000731 0.003615
Mitotic Spindle 0.000328 0.000412 0.000237 0.024949 0.000383 0.002722 0.000742 0.032960 0.000363 0.001575 0.000686 0.025716
None 0.015754 0.080228 0.001560 0.003411 0.004696 0.011302 0.002829 0.005195 0.008856 0.045513 0.002688 0.003582
Nuclear Periphery 0.000200 0.000540 0.000722 0.001614 0.000385 0.001093 0.000650 0.000815 0.000315 0.000819 0.000658 0.001537
Nuclear Periphery Foci 0.000123 0.001202 0.000559 0.000467 0.000198 0.000432 0.000122 0.000122 0.000170 0.000814 0.000171 0.000434
Nucleolus 0.005052 0.018027 0.003414 0.001365 0.003604 0.005709 0.002248 0.001369 0.004148 0.011823 0.002378 0.001366
Nucleus 0.944431* 0.806580* 0.954421* 0.906086* 0.974596* 0.939900* 0.969668* 0.910720* 0.963247* 0.873727* 0.967970* 0.906530*
Peroxisomes 0.000247 0.003139 0.000377 0.000350 0.000289 0.000772 0.000099 0.000683 0.000274 0.001947 0.000130 0.000382
Vacuole 0.000505 0.002382 0.007063 0.006590 0.000461 0.003314 0.001819 0.006547 0.000478 0.002852 0.002403 0.006586
Vacuole Periphery 0.000160 0.000397 0.000155 0.001257 0.000137 0.000876 0.000070 0.000099 0.000146 0.000638 0.000079 0.001146

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.19 -8.25 -8.32 -4.72 3.13 -1.15 -11.79 -4.25 -4.06 -2.92 -5.66 -15.13 -8.79 -6.70 -3.18
Bud Neck -4.84 -7.92 -15.92 -10.90 -9.00 -3.10 -10.02 -7.42 -6.49 -2.94 -5.58 -11.38 -16.78 -13.54 -8.26
Bud Site -5.45 -5.43 -5.40 -1.98 -3.59 -1.33 -2.73 -1.85 -1.68 -1.68 -4.80 -4.81 -5.57 -3.50 -4.76
Cell Periphery -5.70 -3.03 -2.41 5.15 0.78 -3.22 -0.89 -1.97 1.85 -0.66 -6.27 -1.52 -2.27 5.27 -0.54
Cytoplasm -4.72 4.87 5.53 11.19 -0.48 -3.98 1.87 -1.36 0.74 -2.03 -7.24 6.03 3.61 10.70 -2.95
Cytoplasmic Foci -6.57 4.43 1.38 7.01 -1.95 -2.45 2.57 2.43 4.38 -1.08 -7.01 4.53 0.85 7.12 -2.18
Eisosomes -8.61 -7.93 -14.89 4.45 4.26 -2.72 -8.64 -6.95 -5.88 -3.93 -8.83 -11.76 -16.12 0.86 -1.58
Endoplasmic Reticulum -13.65 -11.11 -23.76 -11.05 3.03 -3.31 -15.14 -6.45 -5.34 -3.22 -9.95 -20.39 -22.06 -13.74 -6.20
Endosome -6.13 -3.26 -2.92 0.56 -1.49 -2.53 -0.17 -2.31 1.86 -2.94 -4.14 -1.18 -2.77 1.16 -2.51
Golgi -3.56 -0.48 -2.34 1.48 -2.27 -1.45 1.60 1.28 2.22 -2.03 -3.32 1.59 -1.68 1.32 -2.54
Lipid Particles -4.67 -1.80 0.89 4.84 2.27 -2.08 1.64 0.00 2.16 -2.96 -4.61 1.59 1.07 5.12 -1.05
Mitochondria -0.93 0.92 -0.61 0.53 -3.30 -1.50 0.61 -1.04 1.11 -5.23 -1.99 1.23 -1.78 0.29 -3.87
Mitotic Spindle -0.24 -0.38 -6.25 -6.25 -6.20 -2.12 -1.35 -2.38 -2.15 -2.31 -2.11 -1.44 -6.69 -6.11 -6.36
None -10.65 6.59 6.00 13.59 -2.45 -3.54 2.61 -0.11 3.34 -2.30 -11.56 6.41 5.96 13.86 -1.44
Nuclear Periphery -7.00 -7.25 -8.63 -7.35 -5.67 -3.93 -8.50 -6.01 -3.08 -4.22 -5.30 -11.43 -8.83 -6.48 -6.45
Nuclear Periphery Foci -4.62 -1.83 -1.52 3.90 1.04 -1.67 0.00 -1.32 0.72 -1.45 -4.76 -0.61 -2.04 3.13 -1.53
Nucleolus -7.13 1.31 2.68 10.50 1.74 -2.72 2.35 3.85 5.65 1.13 -8.15 2.96 3.77 11.29 0.74
Nucleus 13.84 -0.58 5.30 -10.34 5.91 6.45 2.98 5.78 3.50 5.21 15.83 -0.04 11.32 -4.61 11.86
Peroxisomes -4.74 -1.28 0.00 4.77 1.55 -2.12 1.96 -0.50 1.23 -1.25 -5.16 1.65 0.13 5.15 -1.21
Vacuole -3.82 -6.84 -8.83 -7.55 -2.09 -3.76 -6.61 -3.91 -3.54 -3.56 -5.39 -8.01 -9.77 -7.85 -7.23
Vacuole Periphery -2.91 -0.25 -2.12 -1.19 -2.12 -2.57 1.14 -0.16 2.57 -2.58 -3.29 1.35 -2.23 0.01 -2.52
Standard name
Human Ortholog
Description RNA binding protein; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Prp28

Prp28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available