Standard name | |
---|---|
Human Ortholog | |
Description | RNA binding protein; involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 5 | 1 | 3 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 7 | 1 | 0 | 8 | 10 | 9 | 23 | 4 | 6 | 12 | 5 | – | – | – | 20 | 9 | 12 | 0 | 5 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | – | – | 3 | 4 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 3 | 2 | 3 | 0 | 4 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
Mitochondria | 21 | 21 | 0 | 4 | 0 | 10 | 19 | 18 | 40 | 31 | 70 | 29 | – | – | – | 15 | 5 | 5 | 0 | 3 | 1 | 0 | 0 | 0 |
Nucleus | 131 | 315 | 25 | 24 | 168 | 134 | 194 | 223 | 96 | 121 | 137 | 157 | – | – | – | 277 | 193 | 219 | 123 | 312 | 52 | 13 | 17 | 45 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 1 | 4 | – | – | – | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 6 | 5 | 8 | 8 | 5 | – | – | – | 6 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | – | – | – | 2 | 0 | 4 | 1 | 1 | 1 | 1 | 0 | 1 |
Vac/Vac Membrane | 1 | 15 | 2 | 0 | 2 | 3 | 3 | 2 | 1 | 0 | 2 | 2 | – | – | – | 22 | 12 | 18 | 3 | 23 | 5 | 0 | 0 | 3 |
Unique Cell Count | 135 | 338 | 28 | 26 | 178 | 152 | 207 | 264 | 111 | 140 | 166 | 172 | 332 | 217 | 258 | 131 | 360 | 69 | 20 | 22 | 54 | |||
Labelled Cell Count | 155 | 360 | 28 | 31 | 180 | 160 | 227 | 279 | 151 | 174 | 231 | 206 | 351 | 230 | 270 | 131 | 360 | 69 | 20 | 22 | 54 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.7 | 6.9 | 5.5 | 6.9 | 5.8 | 5.7 | 6.3 | 4.4 | 5.0 | 4.7 | 5.5 | – | – | – | 8.8 | 8.6 | 8.1 | – | – | 5.6 |
Std Deviation (1e-4) | 0.8 | 1.0 | 1.2 | 1.4 | 2.2 | 1.8 | 1.7 | 2.4 | 1.3 | 1.1 | 1.6 | 2.4 | – | – | – | 2.4 | 2.1 | 2.2 | – | – | 0.9 |
Intensity Change (Log2) | – | – | – | -0.34 | -0.0 | -0.24 | -0.27 | -0.13 | -0.64 | -0.45 | -0.55 | -0.33 | – | – | – | 0.35 | 0.32 | 0.24 | – | – | -0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | 0.4 | 1.0 | -0.2 | 0.9 | -0.7 | -0.4 | -0.4 | -0.9 | 0.3 | – | – | – | -0.8 | -0.1 | -0.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0062 | 1.8777 | 1.8599 | 1.4151 | 1.5441 | 1.7172 | 0.7972 | 1.4757 | 1.2734 | 1.1726 | 1.605 | 1.3193 | 1.0238 | 1.312 | 0.6662 | 0.7063 | 0.6119 | 0.8986 |
Actin | 0.0084 | 0 | 0.0014 | 0.0002 | 0.0039 | 0.002 | 0.032 | 0 | 0.0049 | 0.0247 | 0.0206 | 0.0043 | 0.0826 | 0 | 0.016 | 0 | 0.0101 | 0.0074 |
Bud | 0.0012 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0006 | 0.0006 | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Bud Neck | 0.0019 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0007 | 0.0039 | 0 | 0.0011 | 0.0019 | 0.001 | 0.0011 | 0.0018 | 0 | 0.0004 | 0 | 0.0007 | 0.0006 |
Bud Periphery | 0.0018 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0005 | 0.0005 | 0 | 0.0014 | 0.0007 | 0.0008 | 0.0006 | 0.0008 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0001 |
Bud Site | 0.002 | 0.0001 | 0.0007 | 0.0002 | 0.0004 | 0.0005 | 0.0097 | 0 | 0.0022 | 0.0077 | 0.0046 | 0.0025 | 0.0016 | 0 | 0.0007 | 0 | 0.0004 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0016 | 0.0004 | 0.0007 | 0.0002 | 0.001 | 0.0003 | 0.0093 | 0.0001 | 0.0014 | 0.0019 | 0.0012 | 0.002 | 0.0039 | 0.0005 | 0.0006 | 0.0019 | 0.0016 | 0.0006 |
Cytoplasmic Foci | 0.0048 | 0.0001 | 0.0039 | 0.0011 | 0.0226 | 0.0007 | 0.012 | 0 | 0.0047 | 0.0091 | 0.0158 | 0.0064 | 0.0067 | 0 | 0.0007 | 0 | 0.0115 | 0.001 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0006 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0033 | 0 | 0.0009 | 0.0004 | 0.0008 | 0.0007 | 0.0023 | 0 | 0.0006 | 0.0008 | 0.0006 | 0.0026 | 0.0049 | 0 | 0.0007 | 0 | 0.0034 | 0.0018 |
Endosome | 0.0177 | 0 | 0.0053 | 0.0028 | 0.0126 | 0.0026 | 0.0096 | 0 | 0.0079 | 0.0077 | 0.0537 | 0.0106 | 0.0198 | 0 | 0.0057 | 0 | 0.0206 | 0.0032 |
Golgi | 0.0039 | 0 | 0.0016 | 0.0005 | 0.0031 | 0.0019 | 0.0043 | 0 | 0.0036 | 0.0045 | 0.0179 | 0.0023 | 0.0077 | 0 | 0.0012 | 0 | 0.0066 | 0.0012 |
Lipid Particles | 0.0181 | 0 | 0.0072 | 0.0135 | 0.0087 | 0.0048 | 0.0106 | 0 | 0.0014 | 0.0176 | 0.0716 | 0.0043 | 0.0087 | 0 | 0.0012 | 0 | 0.0203 | 0.001 |
Mitochondria | 0.0222 | 0.0001 | 0.0052 | 0.0039 | 0.002 | 0.0065 | 0.0049 | 0.0001 | 0.0162 | 0.0054 | 0.0331 | 0.0025 | 0.0055 | 0.0002 | 0.0021 | 0.0001 | 0.0047 | 0.0003 |
None | 0.0023 | 0.0001 | 0.0009 | 0.0004 | 0.0016 | 0.0001 | 0.0035 | 0 | 0.0003 | 0.0121 | 0.0004 | 0.0004 | 0.0037 | 0.0001 | 0.0005 | 0.0208 | 0.0043 | 0.0005 |
Nuclear Periphery | 0.0305 | 0.0036 | 0.006 | 0.0107 | 0.0434 | 0.006 | 0.004 | 0.0015 | 0.0046 | 0.004 | 0.0027 | 0.0081 | 0.0251 | 0.0015 | 0.0021 | 0.0004 | 0.0243 | 0.01 |
Nucleolus | 0.0136 | 0.0087 | 0.009 | 0.0089 | 0.0174 | 0.0243 | 0.0073 | 0.0045 | 0.005 | 0.0134 | 0.0338 | 0.0149 | 0.0051 | 0.0052 | 0.0085 | 0.0022 | 0.0157 | 0.0093 |
Nucleus | 0.8264 | 0.9798 | 0.9359 | 0.9468 | 0.7809 | 0.9386 | 0.8678 | 0.9932 | 0.9342 | 0.8644 | 0.7109 | 0.9298 | 0.7839 | 0.9892 | 0.9551 | 0.9728 | 0.8272 | 0.9566 |
Peroxisomes | 0.011 | 0 | 0.0022 | 0.0011 | 0.0125 | 0.0012 | 0.0087 | 0 | 0.0026 | 0.0171 | 0.0142 | 0.0009 | 0.0028 | 0 | 0.0004 | 0 | 0.0387 | 0.0012 |
Punctate Nuclear | 0.0168 | 0.0066 | 0.0152 | 0.0059 | 0.0867 | 0.0075 | 0.0066 | 0.0004 | 0.0022 | 0.0023 | 0.0079 | 0.0039 | 0.0307 | 0.0028 | 0.0005 | 0.0017 | 0.0067 | 0.0044 |
Vacuole | 0.0067 | 0.0002 | 0.0015 | 0.0013 | 0.0004 | 0.0005 | 0.0016 | 0.0001 | 0.004 | 0.0031 | 0.0061 | 0.0018 | 0.0017 | 0.0002 | 0.0017 | 0 | 0.0008 | 0.0002 |
Vacuole Periphery | 0.0052 | 0.0001 | 0.0012 | 0.0015 | 0.0011 | 0.0003 | 0.0005 | 0 | 0.0008 | 0.0006 | 0.0019 | 0.0006 | 0.0018 | 0.0001 | 0.0009 | 0 | 0.0015 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.679 | 7.8151 | 7.456 | 8.7799 | 6.0722 | 14.9785 | 11.0061 | 11.0473 | 7.258 | 11.6528 |
Translational Efficiency | 1.45 | 1.5927 | 1.7513 | 1.0617 | 1.701 | 0.7194 | 1.0848 | 0.9212 | 1.2137 | 1.0004 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|