Standard name | |
---|---|
Human Ortholog | |
Description | Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 48 | 79 | 21 | 26 | 17 | 12 | – | 33 | 18 | 13 | 39 | 28 | 43 | 36 | 18 | 29 | 7 | 9 | 9 | 18 | 15 | 7 | 11 | 17 |
Bud | 5 | 1 | 3 | 4 | 5 | 11 | – | 20 | 26 | 24 | 39 | 40 | 0 | 0 | 0 | 5 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 2 | 0 | 1 | 5 | – | 3 | 5 | 4 | 5 | 16 | 2 | 3 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 11 | 14 | 19 | 19 | 22 | 23 | – | 45 | 43 | 52 | 47 | 27 | 10 | 7 | 13 | 12 | 7 | 13 | 0 | 0 | 1 | 1 | 0 | 1 |
Endoplasmic Reticulum | 6 | 13 | 7 | 10 | 6 | 12 | – | 12 | 15 | 18 | 23 | 16 | 28 | 26 | 18 | 7 | 0 | 2 | 0 | 1 | 2 | 0 | 2 | 2 |
Endosome | 12 | 4 | 13 | 38 | 40 | 50 | – | 103 | 46 | 37 | 35 | 90 | 4 | 0 | 2 | 24 | 16 | 13 | 28 | 53 | 34 | 41 | 61 | 50 |
Golgi | 278 | 328 | 249 | 222 | 284 | 161 | – | 209 | 100 | 59 | 65 | 68 | 241 | 176 | 197 | 184 | 68 | 93 | 299 | 348 | 279 | 158 | 406 | 380 |
Mitochondria | 117 | 173 | 156 | 115 | 78 | 226 | – | 168 | 246 | 274 | 404 | 375 | 51 | 23 | 13 | 16 | 14 | 10 | 2 | 12 | 5 | 6 | 7 | 7 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 1 | – | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 1 | 3 | 3 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 10 | 12 | 12 | 12 | 7 | 21 | – | 29 | 14 | 10 | 23 | 16 | 2 | 2 | 1 | 5 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 3 | 2 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 4 | 4 | 8 | 4 |
SpindlePole | 1 | 0 | 0 | 2 | 5 | 3 | – | 11 | 6 | 4 | 6 | 13 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 66 | 67 | 31 | 17 | 63 | 43 | – | 65 | 32 | 25 | 35 | 36 | 54 | 42 | 59 | 30 | 18 | 19 | 7 | 23 | 4 | 3 | 5 | 6 |
Unique Cell Count | 345 | 422 | 330 | 310 | 405 | 417 | 518 | 412 | 424 | 554 | 562 | 274 | 211 | 238 | 255 | 111 | 125 | 354 | 467 | 352 | 229 | 509 | 473 | |
Labelled Cell Count | 555 | 693 | 513 | 465 | 530 | 571 | 703 | 558 | 525 | 727 | 734 | 437 | 315 | 322 | 317 | 141 | 162 | 354 | 467 | 352 | 229 | 509 | 473 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.1 | 18.7 | 15.2 | 14.5 | 13.2 | 11.2 | – | 10.3 | 9.3 | 8.6 | 8.8 | 8.7 | 18.1 | 21.1 | 20.9 | 12.0 | 13.0 | 15.8 | 15.4 | 17.1 | 17.6 |
Std Deviation (1e-4) | 3.1 | 3.1 | 2.6 | 2.5 | 2.2 | 1.9 | – | 1.6 | 1.6 | 1.5 | 1.7 | 1.5 | 3.4 | 4.1 | 4.2 | 2.4 | 3.5 | 3.4 | 2.8 | 3.1 | 3.2 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.2 | -0.44 | – | -0.57 | -0.7 | -0.82 | -0.79 | -0.8 | 0.25 | 0.48 | 0.46 | -0.34 | -0.23 | 0.05 | 0.02 | 0.17 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.0 | -1.3 | -2.3 | – | 0 | -1.2 | -2.2 | 0.4 | -0.9 | 3.7 | 4.0 | 0.6 | 2.1 | 0 | 0.3 |
Bud | 0 | 0 | 1.7 | – | 2.6 | 3.8 | 3.5 | 4.1 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -0.2 | -0.1 | – | 1.6 | 2.3 | 3.0 | 1.5 | -0.6 | -1.2 | -1.3 | -0.2 | -0.6 | 0.2 | 1.7 |
Endoplasmic Reticulum | 0.9 | -0.7 | 0.7 | – | 0.2 | 1.2 | 1.6 | 1.6 | 0.7 | 4.2 | 4.8 | 3.1 | 0.5 | 0 | 0 |
Endosome | 3.9 | 3.1 | 3.9 | – | 6.6 | 3.6 | 2.6 | 1.5 | 5.4 | -1.8 | -2.9 | -2.3 | 2.7 | 3.9 | 2.7 |
Golgi | -1.1 | -1.6 | -10.0 | – | -10.0 | -13.9 | -17.1 | -19.1 | -19.1 | 3.9 | 2.2 | 2.1 | -0.9 | -2.9 | -0.2 |
Mitochondria | -2.6 | -8.1 | 1.9 | – | -4.3 | 3.4 | 4.8 | 7.7 | 5.7 | -7.4 | -8.8 | -10.8 | -10.8 | -6.5 | -7.8 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.2 | -1.6 | 0.9 | – | 1.3 | -0.2 | -1.0 | 0.4 | -0.7 | -2.4 | -1.9 | -2.5 | -1.2 | 1.6 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | 2.5 | 0.4 | – | 1.4 | -0.8 | -1.8 | -1.7 | -1.6 | 3.6 | 3.5 | 5.0 | 0.9 | 2.0 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.3508 | 21.057 | 18.6133 | 17.0566 | 16.1044 | 19.5522 | 12.016 | 15.9225 | 14.448 | 12.0671 | 12.6804 | 13.9254 | 15.9323 | 21.0218 | 18.1142 | 18.6444 | 17.5878 | 19.7108 |
Actin | 0.0361 | 0.0012 | 0.003 | 0.0295 | 0.0761 | 0.0046 | 0.0401 | 0.0112 | 0.0015 | 0.0016 | 0.0013 | 0.0123 | 0.0392 | 0.0005 | 0.0029 | 0.0166 | 0.0095 | 0.0026 |
Bud | 0.0007 | 0.0005 | 0.0001 | 0.0007 | 0.0006 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0009 | 0.0001 | 0.0002 | 0.002 | 0.0004 | 0.0001 | 0.0026 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 |
Bud Periphery | 0.0013 | 0.0003 | 0.0001 | 0.0005 | 0.0009 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 |
Bud Site | 0.0021 | 0.0003 | 0.0002 | 0.0333 | 0.0016 | 0 | 0.0015 | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0023 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.0034 | 0.0023 | 0.0025 | 0.0019 | 0.0069 | 0.0004 | 0.011 | 0.0027 | 0.0006 | 0.0006 | 0.0009 | 0.0007 | 0.0023 | 0.003 | 0.001 | 0.0011 | 0.0007 | 0.0005 |
Cytoplasmic Foci | 0.0281 | 0.0265 | 0.0224 | 0.0253 | 0.0197 | 0.0079 | 0.0347 | 0.0438 | 0.029 | 0.0244 | 0.0448 | 0.0193 | 0.039 | 0.0281 | 0.0275 | 0.0235 | 0.0227 | 0.0113 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0029 | 0 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0026 | 0.0006 | 0.001 | 0.0011 | 0.0061 | 0.0006 | 0.0143 | 0.0005 | 0.0003 | 0.0003 | 0.0009 | 0.0011 | 0.0025 | 0.0006 | 0.0005 | 0.0009 | 0.0005 | 0.0004 |
Endosome | 0.1647 | 0.2543 | 0.2184 | 0.2889 | 0.2676 | 0.1352 | 0.2348 | 0.1964 | 0.1877 | 0.3149 | 0.3719 | 0.1671 | 0.2248 | 0.2609 | 0.2347 | 0.2879 | 0.2587 | 0.1331 |
Golgi | 0.5822 | 0.6558 | 0.6654 | 0.4644 | 0.4548 | 0.8196 | 0.5694 | 0.7074 | 0.756 | 0.5645 | 0.5053 | 0.7652 | 0.5791 | 0.6701 | 0.6745 | 0.6061 | 0.6538 | 0.8312 |
Lipid Particles | 0.0473 | 0.0045 | 0.0144 | 0.0175 | 0.0205 | 0.0014 | 0.0039 | 0.0174 | 0.0026 | 0.0041 | 0.0233 | 0.0028 | 0.0199 | 0.0068 | 0.0062 | 0.0111 | 0.007 | 0.0013 |
Mitochondria | 0.046 | 0.015 | 0.0215 | 0.0687 | 0.0562 | 0.0084 | 0.0099 | 0.0075 | 0.0155 | 0.057 | 0.0078 | 0.0218 | 0.0477 | 0.0054 | 0.0152 | 0.0248 | 0.0217 | 0.01 |
None | 0.0042 | 0.0001 | 0.0004 | 0.0012 | 0.0032 | 0 | 0.0076 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0057 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0 |
Nuclear Periphery | 0.0089 | 0.0003 | 0.003 | 0.001 | 0.0113 | 0.0004 | 0.014 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0013 | 0.0031 | 0.0018 | 0.0004 | 0.0004 | 0.0006 | 0.0001 |
Nucleolus | 0.0035 | 0.0002 | 0.0001 | 0.0003 | 0.001 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0005 | 0.0034 | 0 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0036 | 0.0002 | 0.0057 | 0.0002 | 0.0022 | 0.0001 | 0.0049 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0 |
Peroxisomes | 0.0108 | 0.0011 | 0.0044 | 0.0044 | 0.0192 | 0 | 0.0007 | 0.0027 | 0.0017 | 0.0011 | 0.0049 | 0.0005 | 0.0049 | 0.0003 | 0.0095 | 0.0015 | 0.0004 | 0.0002 |
Punctate Nuclear | 0.0195 | 0 | 0.0009 | 0.0007 | 0.0022 | 0 | 0.0045 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0032 | 0.0006 | 0.0015 | 0.0002 | 0 | 0 |
Vacuole | 0.0071 | 0.0146 | 0.0075 | 0.0214 | 0.0127 | 0.0047 | 0.0182 | 0.0037 | 0.0013 | 0.0171 | 0.0139 | 0.0021 | 0.0101 | 0.0043 | 0.0081 | 0.0066 | 0.0072 | 0.0028 |
Vacuole Periphery | 0.0264 | 0.0219 | 0.0284 | 0.0367 | 0.036 | 0.0159 | 0.0228 | 0.0046 | 0.0034 | 0.0138 | 0.0232 | 0.0045 | 0.0143 | 0.012 | 0.0148 | 0.0177 | 0.0164 | 0.0061 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 83.4164 | 88.5375 | 87.7045 | 105.0272 | 64.5178 | 94.1819 | 137.2135 | 138.8782 | 147.8954 | 148.6883 |
Translational Efficiency | 0.8193 | 0.9042 | 0.9211 | 0.7846 | 0.7354 | 0.8496 | 0.7882 | 0.6942 | 0.7189 | 0.5989 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
444 | 1556 | 329 | 1346 | 1249 | 1829 | 1827 | 1527 | 1693 | 3385 | 2156 | 2873 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1402.73 | 1714.35 | 2026.61 | 1660.00 | 1390.11 | 1329.99 | 1712.94 | 1579.77 | 1393.42 | 1506.67 | 1760.81 | 1617.36 |
Standard Deviation | 224.83 | 349.26 | 262.14 | 333.60 | 218.05 | 218.99 | 260.51 | 255.00 | 219.92 | 344.49 | 284.11 | 297.16 |
Intensity Change Log 2 | — | 0.289424 | 0.530831 | 0.242946 | — | -0.063784 | 0.301276 | 0.184515 | — | 0.124397 | 0.421132 | 0.214158 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.011046 | 0.003673 | 0.009367 | 0.012025 | 0.000764 | 0.000908 | 0.003901 | 0.007011 | 0.003460 | 0.002179 | 0.004735 | 0.009360 |
Bud Neck | 0.001191 | 0.000134 | 0.004865 | 0.000873 | 0.000059 | 0.000062 | 0.000242 | 0.000216 | 0.000356 | 0.000095 | 0.000948 | 0.000524 |
Bud Site | 0.012542 | 0.000905 | 0.002423 | 0.006904 | 0.000550 | 0.000787 | 0.000479 | 0.002497 | 0.003695 | 0.000841 | 0.000776 | 0.004562 |
Cell Periphery | 0.000631 | 0.000121 | 0.000393 | 0.000441 | 0.000112 | 0.000068 | 0.000096 | 0.000210 | 0.000248 | 0.000092 | 0.000141 | 0.000318 |
Cytoplasm | 0.002134 | 0.000194 | 0.000195 | 0.001679 | 0.000282 | 0.000446 | 0.000107 | 0.001117 | 0.000768 | 0.000330 | 0.000121 | 0.001380 |
Cytoplasmic Foci | 0.083535 | 0.041070 | 0.038587 | 0.029978 | 0.048198 | 0.092520 | 0.025979 | 0.035499 | 0.057465 | 0.068870 | 0.027903 | 0.032912 |
Eisosomes | 0.000073 | 0.000053 | 0.000111 | 0.000106 | 0.000033 | 0.000026 | 0.000050 | 0.000095 | 0.000044 | 0.000038 | 0.000060 | 0.000100 |
Endoplasmic Reticulum | 0.004559 | 0.001305 | 0.002079 | 0.001924 | 0.000594 | 0.000559 | 0.000831 | 0.000783 | 0.001634 | 0.000902 | 0.001021 | 0.001317 |
Endosome | 0.139406 | 0.039625 | 0.052623 | 0.073114 | 0.056864 | 0.084854 | 0.030929 | 0.071497 | 0.078511 | 0.064063 | 0.034240 | 0.072255 |
Golgi | 0.601512* | 0.873977* | 0.693689* | 0.769622* | 0.821218* | 0.781035* | 0.874683* | 0.827839* | 0.763599* | 0.823758* | 0.847064* | 0.800564* |
Lipid Particles | 0.004853 | 0.007538 | 0.032067 | 0.011351 | 0.002693 | 0.004104 | 0.012567 | 0.006026 | 0.003260 | 0.005682 | 0.015543 | 0.008520 |
Mitochondria | 0.010232 | 0.006535 | 0.009551 | 0.015290 | 0.028343 | 0.010727 | 0.005148 | 0.012599 | 0.023593 | 0.008800 | 0.005820 | 0.013860 |
Mitotic Spindle | 0.005973 | 0.000267 | 0.005394 | 0.004927 | 0.000209 | 0.000397 | 0.000301 | 0.001198 | 0.001721 | 0.000337 | 0.001078 | 0.002945 |
None | 0.000062 | 0.000054 | 0.000199 | 0.000120 | 0.000073 | 0.000065 | 0.000053 | 0.000044 | 0.000070 | 0.000060 | 0.000075 | 0.000080 |
Nuclear Periphery | 0.000437 | 0.000075 | 0.003205 | 0.000331 | 0.000094 | 0.000051 | 0.000327 | 0.000067 | 0.000184 | 0.000062 | 0.000766 | 0.000191 |
Nuclear Periphery Foci | 0.002260 | 0.000191 | 0.004192 | 0.001501 | 0.000184 | 0.000156 | 0.000554 | 0.000301 | 0.000728 | 0.000172 | 0.001110 | 0.000863 |
Nucleolus | 0.000026 | 0.000008 | 0.000067 | 0.000026 | 0.000033 | 0.000010 | 0.000006 | 0.000004 | 0.000031 | 0.000009 | 0.000016 | 0.000014 |
Nucleus | 0.000392 | 0.000011 | 0.000049 | 0.000028 | 0.000028 | 0.000016 | 0.000008 | 0.000007 | 0.000123 | 0.000014 | 0.000014 | 0.000017 |
Peroxisomes | 0.001636 | 0.000246 | 0.000937 | 0.000560 | 0.000111 | 0.000360 | 0.000126 | 0.000338 | 0.000511 | 0.000308 | 0.000250 | 0.000442 |
Vacuole | 0.077541 | 0.008949 | 0.070469 | 0.037878 | 0.011085 | 0.008410 | 0.017609 | 0.014997 | 0.028514 | 0.008658 | 0.025675 | 0.025716 |
Vacuole Periphery | 0.039959 | 0.015069 | 0.069538 | 0.031322 | 0.028471 | 0.014440 | 0.026003 | 0.017657 | 0.031484 | 0.014729 | 0.032646 | 0.024059 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.35 | 0.44 | -0.30 | -4.37 | -0.93 | -0.87 | -4.88 | -4.88 | -4.76 | -2.18 | 1.46 | -1.22 | -4.37 | -6.38 | -3.69 |
Bud Neck | 5.08 | -4.11 | 1.29 | -6.21 | 4.54 | -0.24 | -5.27 | -4.06 | -4.05 | 0.50 | 4.58 | -3.91 | -2.10 | -7.18 | 2.75 |
Bud Site | 4.78 | 4.08 | 2.20 | -7.70 | -5.02 | -1.38 | 0.86 | -5.28 | -4.35 | -5.51 | 4.33 | 4.43 | -1.13 | -8.80 | -9.05 |
Cell Periphery | 2.73 | 1.25 | 0.97 | -7.43 | -0.92 | 3.13 | 1.16 | -3.81 | -6.08 | -4.93 | 3.08 | 2.10 | -1.32 | -9.49 | -7.35 |
Cytoplasm | 4.36 | 4.35 | 0.92 | -6.16 | -6.12 | -1.54 | 3.79 | -2.37 | -1.87 | -2.88 | 3.34 | 5.23 | -2.44 | -4.75 | -5.81 |
Cytoplasmic Foci | 7.74 | 6.97 | 9.87 | 5.62 | 2.26 | -15.69 | 10.98 | 5.96 | 22.25 | -5.83 | -4.76 | 13.49 | 11.61 | 21.00 | -3.61 |
Eisosomes | 1.48 | -1.80 | -1.86 | -4.08 | 0.20 | 2.60 | -2.83 | -4.72 | -5.34 | -3.19 | 1.26 | -2.41 | -5.79 | -6.73 | -3.90 |
Endoplasmic Reticulum | 1.67 | 1.26 | 1.32 | -1.43 | 0.24 | 0.33 | -2.12 | -1.50 | -1.95 | 0.40 | 1.40 | 1.18 | 0.57 | -1.95 | -1.42 |
Endosome | 13.44 | 10.67 | 8.48 | -10.27 | -4.42 | -8.92 | 10.17 | -4.52 | 4.08 | -15.03 | 4.76 | 15.13 | 1.90 | -3.51 | -17.18 |
Golgi | -18.01 | -4.49 | -10.37 | 13.53 | -4.68 | 5.78 | -8.15 | -0.89 | -7.12 | 7.69 | -8.99 | -11.47 | -5.02 | 4.68 | 8.06 |
Lipid Particles | -2.03 | -5.93 | -3.48 | -1.99 | 4.30 | -1.65 | -6.66 | -2.62 | -1.39 | 3.53 | -3.33 | -8.50 | -4.86 | -2.44 | 4.08 |
Mitochondria | 1.22 | 0.21 | -1.55 | -4.16 | -2.33 | 5.94 | 8.49 | 5.16 | -0.94 | -4.62 | 6.48 | 8.14 | 4.06 | -3.51 | -6.28 |
Mitotic Spindle | 3.17 | 0.36 | 0.73 | -5.04 | 0.21 | -1.78 | -1.63 | -3.03 | -2.36 | -2.74 | 2.91 | 1.24 | -1.54 | -5.54 | -3.19 |
None | 0.64 | -6.06 | -3.73 | -5.19 | 3.51 | 0.30 | 2.28 | 3.27 | 0.79 | 2.08 | 0.66 | -0.61 | -1.14 | -1.32 | -0.75 |
Nuclear Periphery | 4.76 | -5.15 | 0.94 | -3.57 | 5.34 | 2.65 | -4.20 | 1.36 | -1.29 | 4.74 | 5.20 | -5.92 | -0.25 | -3.76 | 5.61 |
Nuclear Periphery Foci | 2.77 | -1.45 | 0.91 | -2.15 | 2.46 | 0.84 | -4.37 | -0.99 | -1.24 | 1.57 | 2.80 | -1.25 | -0.27 | -2.37 | 0.69 |
Nucleolus | 2.05 | -2.71 | -0.02 | -2.35 | 2.80 | 0.84 | 0.97 | 1.04 | 0.85 | 2.38 | 1.06 | 0.79 | 0.83 | -1.06 | 0.25 |
Nucleus | 1.23 | 1.10 | 1.17 | -3.77 | 2.65 | 0.56 | 0.96 | 0.98 | 1.06 | 0.79 | 1.33 | 1.33 | 1.29 | -0.72 | -1.74 |
Peroxisomes | 4.46 | 1.82 | 3.46 | -2.35 | 1.50 | -3.18 | -0.60 | -5.34 | 0.26 | -4.44 | 2.00 | 2.87 | 0.72 | -1.71 | -2.98 |
Vacuole | 10.27 | 0.82 | 5.61 | -11.87 | 5.05 | 2.02 | -3.80 | -2.31 | -4.60 | 1.41 | 9.32 | 1.17 | 1.08 | -12.30 | -0.17 |
Vacuole Periphery | 6.36 | -4.62 | 2.05 | -7.67 | 6.95 | 6.38 | 1.06 | 4.74 | -2.04 | 4.74 | 8.82 | -0.52 | 3.61 | -7.15 | 4.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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