Standard name
Human Ortholog
Description Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.19 0.06 0.08 0 0 – 0.06 0 0 0.07 0.05 0.16 0.17 0.08 0.11 0.06 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0.06 0.06 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.05 0.06 – 0.09 0.1 0.12 0.08 0.05 0 0 0.05 0.05 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.12 0.08 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.12 0.1 0.12 – 0.2 0.11 0.09 0.06 0.16 0 0 0 0.09 0.14 0.1 0.08 0.12 0.1 0.18 0.12 0.11
Golgi 0.81 0.78 0.75 0.72 0.7 0.39 – 0.4 0.24 0.14 0.12 0.12 0.88 0.83 0.83 0.72 0.61 0.74 0.85 0.75 0.79 0.69 0.8 0.8
Mitochondria 0.34 0.41 0.47 0.37 0.19 0.54 – 0.32 0.6 0.65 0.73 0.67 0.19 0.11 0.05 0.06 0.13 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 – 0.06 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.16 0.09 0.05 0.16 0.1 – 0.13 0.08 0.06 0.06 0.06 0.2 0.2 0.25 0.12 0.16 0.15 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 48 79 21 26 17 12 – 33 18 13 39 28 43 36 18 29 7 9 9 18 15 7 11 17
Bud 5 1 3 4 5 11 – 20 26 24 39 40 0 0 0 5 0 1 1 1 0 1 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 1 0 1 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 2 0 1 5 – 3 5 4 5 16 2 3 0 2 2 0 0 0 0 0 0 0
Cytoplasm 11 14 19 19 22 23 – 45 43 52 47 27 10 7 13 12 7 13 0 0 1 1 0 1
Endoplasmic Reticulum 6 13 7 10 6 12 – 12 15 18 23 16 28 26 18 7 0 2 0 1 2 0 2 2
Endosome 12 4 13 38 40 50 – 103 46 37 35 90 4 0 2 24 16 13 28 53 34 41 61 50
Golgi 278 328 249 222 284 161 – 209 100 59 65 68 241 176 197 184 68 93 299 348 279 158 406 380
Mitochondria 117 173 156 115 78 226 – 168 246 274 404 375 51 23 13 16 14 10 2 12 5 6 7 7
Nucleus 0 0 0 0 1 1 – 2 2 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 2 – 0 1 3 3 0 1 0 0 1 0 1 0 0 1 0 0 0
Nucleolus 10 12 12 12 7 21 – 29 14 10 23 16 2 2 1 5 8 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 – 3 3 2 1 6 0 0 0 0 0 0 3 2 4 4 8 4
SpindlePole 1 0 0 2 5 3 – 11 6 4 6 13 0 0 1 2 0 0 0 1 0 0 1 0
Vac/Vac Membrane 66 67 31 17 63 43 – 65 32 25 35 36 54 42 59 30 18 19 7 23 4 3 5 6
Unique Cell Count 345 422 330 310 405 417 518 412 424 554 562 274 211 238 255 111 125 354 467 352 229 509 473
Labelled Cell Count 555 693 513 465 530 571 703 558 525 727 734 437 315 322 317 141 162 354 467 352 229 509 473


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.1 18.7 15.2 14.5 13.2 11.2 – 10.3 9.3 8.6 8.8 8.7 18.1 21.1 20.9 12.0 13.0 15.8 15.4 17.1 17.6
Std Deviation (1e-4) 3.1 3.1 2.6 2.5 2.2 1.9 – 1.6 1.6 1.5 1.7 1.5 3.4 4.1 4.2 2.4 3.5 3.4 2.8 3.1 3.2
Intensity Change (Log2) – – – -0.06 -0.2 -0.44 – -0.57 -0.7 -0.82 -0.79 -0.8 0.25 0.48 0.46 -0.34 -0.23 0.05 0.02 0.17 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -1.3 -2.3 – 0 -1.2 -2.2 0.4 -0.9 3.7 4.0 0.6 2.1 0 0.3
Bud 0 0 1.7 – 2.6 3.8 3.5 4.1 4.2 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 2.3 0 0 0 0 0 0
Cytoplasm 0.2 -0.2 -0.1 – 1.6 2.3 3.0 1.5 -0.6 -1.2 -1.3 -0.2 -0.6 0.2 1.7
Endoplasmic Reticulum 0.9 -0.7 0.7 – 0.2 1.2 1.6 1.6 0.7 4.2 4.8 3.1 0.5 0 0
Endosome 3.9 3.1 3.9 – 6.6 3.6 2.6 1.5 5.4 -1.8 -2.9 -2.3 2.7 3.9 2.7
Golgi -1.1 -1.6 -10.0 – -10.0 -13.9 -17.1 -19.1 -19.1 3.9 2.2 2.1 -0.9 -2.9 -0.2
Mitochondria -2.6 -8.1 1.9 – -4.3 3.4 4.8 7.7 5.7 -7.4 -8.8 -10.8 -10.8 -6.5 -7.8
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 -1.6 0.9 – 1.3 -0.2 -1.0 0.4 -0.7 -2.4 -1.9 -2.5 -1.2 1.6 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 2.5 0.4 – 1.4 -0.8 -1.8 -1.7 -1.6 3.6 3.5 5.0 0.9 2.0 1.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.3508 21.057 18.6133 17.0566 16.1044 19.5522 12.016 15.9225 14.448 12.0671 12.6804 13.9254 15.9323 21.0218 18.1142 18.6444 17.5878 19.7108
Actin 0.0361 0.0012 0.003 0.0295 0.0761 0.0046 0.0401 0.0112 0.0015 0.0016 0.0013 0.0123 0.0392 0.0005 0.0029 0.0166 0.0095 0.0026
Bud 0.0007 0.0005 0.0001 0.0007 0.0006 0.0001 0.0005 0.0003 0.0001 0 0.0001 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0
Bud Neck 0.0009 0.0001 0.0002 0.002 0.0004 0.0001 0.0026 0.0002 0.0001 0.0001 0.0001 0.0002 0.0005 0.0001 0.0004 0.0002 0.0001 0.0001
Bud Periphery 0.0013 0.0003 0.0001 0.0005 0.0009 0.0002 0.0007 0.0002 0.0001 0 0.0001 0.0002 0.0005 0.0001 0.0001 0.0002 0.0002 0
Bud Site 0.0021 0.0003 0.0002 0.0333 0.0016 0 0.0015 0.0008 0.0001 0 0.0001 0.0001 0.001 0.0001 0.0023 0.0002 0.0001 0
Cell Periphery 0.0003 0.0002 0.0001 0.0002 0.0004 0.0001 0.0006 0 0 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.0034 0.0023 0.0025 0.0019 0.0069 0.0004 0.011 0.0027 0.0006 0.0006 0.0009 0.0007 0.0023 0.003 0.001 0.0011 0.0007 0.0005
Cytoplasmic Foci 0.0281 0.0265 0.0224 0.0253 0.0197 0.0079 0.0347 0.0438 0.029 0.0244 0.0448 0.0193 0.039 0.0281 0.0275 0.0235 0.0227 0.0113
Eisosomes 0.0003 0 0.0001 0.0002 0.0004 0.0001 0.0029 0 0 0 0 0.0001 0.0003 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0026 0.0006 0.001 0.0011 0.0061 0.0006 0.0143 0.0005 0.0003 0.0003 0.0009 0.0011 0.0025 0.0006 0.0005 0.0009 0.0005 0.0004
Endosome 0.1647 0.2543 0.2184 0.2889 0.2676 0.1352 0.2348 0.1964 0.1877 0.3149 0.3719 0.1671 0.2248 0.2609 0.2347 0.2879 0.2587 0.1331
Golgi 0.5822 0.6558 0.6654 0.4644 0.4548 0.8196 0.5694 0.7074 0.756 0.5645 0.5053 0.7652 0.5791 0.6701 0.6745 0.6061 0.6538 0.8312
Lipid Particles 0.0473 0.0045 0.0144 0.0175 0.0205 0.0014 0.0039 0.0174 0.0026 0.0041 0.0233 0.0028 0.0199 0.0068 0.0062 0.0111 0.007 0.0013
Mitochondria 0.046 0.015 0.0215 0.0687 0.0562 0.0084 0.0099 0.0075 0.0155 0.057 0.0078 0.0218 0.0477 0.0054 0.0152 0.0248 0.0217 0.01
None 0.0042 0.0001 0.0004 0.0012 0.0032 0 0.0076 0 0 0 0.0001 0.0001 0.0057 0.0001 0.0003 0.0003 0.0001 0
Nuclear Periphery 0.0089 0.0003 0.003 0.001 0.0113 0.0004 0.014 0.0001 0.0001 0.0002 0.0008 0.0013 0.0031 0.0018 0.0004 0.0004 0.0006 0.0001
Nucleolus 0.0035 0.0002 0.0001 0.0003 0.001 0.0001 0.0005 0.0001 0 0 0 0 0.0005 0.0034 0 0.0001 0.0001 0
Nucleus 0.0036 0.0002 0.0057 0.0002 0.0022 0.0001 0.0049 0.0001 0 0 0.0001 0.0001 0.001 0.0016 0.0001 0.0001 0.0001 0
Peroxisomes 0.0108 0.0011 0.0044 0.0044 0.0192 0 0.0007 0.0027 0.0017 0.0011 0.0049 0.0005 0.0049 0.0003 0.0095 0.0015 0.0004 0.0002
Punctate Nuclear 0.0195 0 0.0009 0.0007 0.0022 0 0.0045 0 0 0 0.0004 0.0001 0.0032 0.0006 0.0015 0.0002 0 0
Vacuole 0.0071 0.0146 0.0075 0.0214 0.0127 0.0047 0.0182 0.0037 0.0013 0.0171 0.0139 0.0021 0.0101 0.0043 0.0081 0.0066 0.0072 0.0028
Vacuole Periphery 0.0264 0.0219 0.0284 0.0367 0.036 0.0159 0.0228 0.0046 0.0034 0.0138 0.0232 0.0045 0.0143 0.012 0.0148 0.0177 0.0164 0.0061

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.4164 88.5375 87.7045 105.0272 64.5178 94.1819 137.2135 138.8782 147.8954 148.6883
Translational Efficiency 0.8193 0.9042 0.9211 0.7846 0.7354 0.8496 0.7882 0.6942 0.7189 0.5989

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant
Localization
Cell Percentages Golgi (26%), vacuole (0%), mixed (51%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Akr1

Akr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Akr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available