Standard name | |
---|---|
Human Ortholog | |
Description | ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 7 | 13 | 13 | 10 | 3 | – | 8 | 11 | 10 | 27 | 5 | 7 | 5 | 1 | – | – | 1 | 2 | 3 | 1 | 6 | 4 |
Bud | 0 | 0 | 1 | 1 | 2 | 3 | 1 | – | 1 | 2 | 4 | 6 | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 0 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 5 | 4 | 12 | 6 | 10 | – | 5 | 12 | 9 | 21 | 16 | 28 | 30 | 4 | – | – | 0 | 0 | 0 | 0 | 4 | 3 |
Cytoplasm | 1 | 13 | 15 | 17 | 16 | 26 | 29 | – | 35 | 27 | 57 | 37 | 6 | 13 | 11 | 3 | – | – | 0 | 6 | 1 | 1 | 1 | 5 |
Endoplasmic Reticulum | 42 | 114 | 142 | 43 | 124 | 74 | 87 | – | 84 | 104 | 87 | 102 | 162 | 166 | 177 | 1 | – | – | 81 | 188 | 226 | 114 | 165 | 186 |
Endosome | 0 | 0 | 1 | 1 | 0 | 0 | 2 | – | 2 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | – | – | 0 | 0 | 2 | 0 | 1 | 0 |
Golgi | 6 | 3 | 29 | 11 | 27 | 1 | 10 | – | 2 | 1 | 2 | 2 | 11 | 7 | 6 | 5 | – | – | 3 | 3 | 4 | 0 | 3 | 4 |
Mitochondria | 6 | 3 | 1 | 15 | 2 | 47 | 4 | – | 32 | 30 | 41 | 72 | 3 | 1 | 0 | 0 | – | – | 2 | 4 | 5 | 3 | 6 | 1 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 4 | 4 | – | 1 | 4 | 5 | 5 | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 16 | 11 | 22 | 4 | 1 | 3 | 8 | – | 3 | 3 | 5 | 16 | 21 | 19 | 10 | 0 | – | – | 3 | 8 | 7 | 1 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 66 | 67 | 35 | 42 | 46 | 89 | – | 35 | 34 | 33 | 56 | 4 | 7 | 10 | 2 | – | – | 0 | 13 | 5 | 0 | 3 | 5 |
Unique Cell Count | 55 | 190 | 222 | 117 | 184 | 169 | 212 | 169 | 191 | 218 | 263 | 177 | 198 | 209 | 16 | 97 | 236 | 261 | 128 | 198 | 217 | |||
Labelled Cell Count | 86 | 211 | 290 | 146 | 240 | 220 | 247 | 208 | 229 | 253 | 347 | 228 | 248 | 250 | 17 | 97 | 236 | 261 | 128 | 198 | 217 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.2 | 15.4 | 15.0 | 13.9 | 14.2 | 9.1 | 12.7 | – | 10.9 | 10.7 | 10.5 | 10.4 | 14.8 | 15.7 | 14.8 | 19.9 | – | – | 13.7 | 15.3 | 15.9 |
Std Deviation (1e-4) | 3.3 | 3.0 | 2.7 | 3.2 | 2.4 | 2.4 | 2.7 | – | 2.3 | 2.3 | 2.5 | 2.1 | 2.4 | 2.8 | 2.2 | 4.3 | – | – | 2.4 | 2.9 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.08 | -0.72 | -0.24 | – | -0.46 | -0.48 | -0.51 | -0.53 | -0.01 | 0.06 | -0.02 | 0.41 | – | – | -0.13 | 0.03 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.0 | 1.8 | 1.3 | 0 | – | 0.8 | 1.3 | 0.8 | 3.1 | -0.2 | 0.2 | -0.5 | 0 | – | – |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0 | 2.1 | 0 | 1.4 | – | 0 | 2.1 | 1.1 | 2.8 | 3.0 | 4.5 | 4.6 | 0 | – | – |
Cytoplasm | 2.3 | 0.7 | 2.8 | 2.4 | – | 4.1 | 2.5 | 5.5 | 2.6 | -1.5 | -0.1 | -0.7 | 0 | – | – |
Endoplasmic Reticulum | -4.8 | 0.7 | -4.0 | -4.8 | – | -2.8 | -2.0 | -5.0 | -5.5 | 6.4 | 4.6 | 4.9 | -4.6 | – | – |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | -1.0 | 0.5 | -4.6 | -3.0 | – | -4.3 | -4.9 | -5.0 | -5.5 | -2.3 | -3.5 | -3.9 | 0 | – | – |
Mitochondria | 5.1 | 0 | 8.2 | 0 | – | 6.5 | 5.9 | 6.6 | 8.3 | 0 | 0 | 0 | 0 | – | – |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | -2.1 | -4.1 | -3.3 | -2.5 | – | -3.3 | -3.5 | -3.3 | -1.6 | 0.6 | -0.1 | -2.0 | 0 | – | – |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | -0.1 | -1.7 | -0.6 | 2.6 | – | -2.1 | -2.9 | -3.8 | -2.2 | -7.2 | -7.2 | -6.9 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.7458 | 9.8718 | 9.5005 | 9.4128 | 7.3841 | 9.8137 | 12.2165 | 13.5195 | 12.8676 | 13.3075 | 12.2201 | 12.4967 | 15.6688 | 16.1895 | 15.8998 | 15.2428 | 18.0904 | 16.4517 |
Actin | 0.0375 | 0.0006 | 0.0072 | 0.0315 | 0.0035 | 0.008 | 0.0042 | 0.0002 | 0.0145 | 0.0004 | 0.0428 | 0.0102 | 0.0256 | 0.0004 | 0.0066 | 0.0001 | 0.0155 | 0.0026 |
Bud | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0059 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0 |
Bud Neck | 0.0008 | 0.0003 | 0.0007 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0009 | 0.0006 | 0.0004 | 0 | 0.0033 | 0.0001 | 0.0043 | 0.0001 |
Bud Periphery | 0.0007 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0112 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Bud Site | 0.0012 | 0.0002 | 0.0007 | 0.001 | 0.0007 | 0.0001 | 0.0019 | 0.0001 | 0.0052 | 0 | 0.0034 | 0.0001 | 0.0003 | 0 | 0.0146 | 0 | 0.0005 | 0 |
Cell Periphery | 0.0007 | 0.0024 | 0.0003 | 0.0011 | 0.0003 | 0.0001 | 0.0007 | 0.0008 | 0.0006 | 0.0003 | 0.0009 | 0.0002 | 0.0003 | 0.0003 | 0.0009 | 0.0027 | 0.0003 | 0.0001 |
Cytoplasm | 0.0108 | 0.0076 | 0.0093 | 0.0014 | 0.0064 | 0.0015 | 0.0106 | 0.0168 | 0.0143 | 0.0012 | 0.0027 | 0.0039 | 0.0054 | 0.0051 | 0.0229 | 0.0008 | 0.0481 | 0.0255 |
Cytoplasmic Foci | 0.0117 | 0.0005 | 0.0083 | 0.0034 | 0.0106 | 0.0048 | 0.0099 | 0.0011 | 0.0132 | 0.001 | 0.0035 | 0.001 | 0.0105 | 0.0001 | 0.0034 | 0 | 0.0044 | 0.0006 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.004 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.6752 | 0.917 | 0.8709 | 0.8619 | 0.7367 | 0.8629 | 0.7219 | 0.9418 | 0.8841 | 0.91 | 0.839 | 0.8781 | 0.7508 | 0.9787 | 0.9031 | 0.9264 | 0.8842 | 0.9219 |
Endosome | 0.0387 | 0.0045 | 0.0136 | 0.0197 | 0.048 | 0.018 | 0.0507 | 0.0094 | 0.0173 | 0.0188 | 0.0219 | 0.0169 | 0.0164 | 0.0022 | 0.0031 | 0.0023 | 0.006 | 0.0037 |
Golgi | 0.0179 | 0.0028 | 0.0098 | 0.0109 | 0.023 | 0.0093 | 0.0125 | 0.0015 | 0.0137 | 0.0169 | 0.0106 | 0.0098 | 0.0076 | 0.0007 | 0.0011 | 0.0002 | 0.0035 | 0.0007 |
Lipid Particles | 0.0159 | 0.0059 | 0.0116 | 0.0309 | 0.0243 | 0.017 | 0.0078 | 0.0085 | 0.0103 | 0.0053 | 0.0318 | 0.004 | 0.0214 | 0.0004 | 0.0014 | 0.0033 | 0.0031 | 0.0024 |
Mitochondria | 0.0107 | 0.0027 | 0.0149 | 0.006 | 0.0103 | 0.0123 | 0.01 | 0.0016 | 0.0036 | 0.0151 | 0.0084 | 0.0033 | 0.0041 | 0.0005 | 0.0017 | 0.0035 | 0.0003 | 0.0006 |
None | 0.0048 | 0 | 0.0006 | 0.0001 | 0.0182 | 0.0002 | 0.002 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0002 | 0.0189 | 0 | 0.0047 | 0.0001 | 0.0003 | 0.0005 |
Nuclear Periphery | 0.1445 | 0.0428 | 0.037 | 0.0189 | 0.0787 | 0.0576 | 0.1079 | 0.0117 | 0.0134 | 0.0243 | 0.012 | 0.0614 | 0.0972 | 0.0088 | 0.0248 | 0.0461 | 0.0178 | 0.0331 |
Nucleolus | 0.0006 | 0 | 0.0003 | 0 | 0.0025 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0011 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 |
Nucleus | 0.007 | 0.0018 | 0.0016 | 0.0005 | 0.0053 | 0.0017 | 0.0397 | 0.0008 | 0.0008 | 0.0016 | 0.0004 | 0.0038 | 0.0046 | 0.0003 | 0.001 | 0.0011 | 0.0007 | 0.0028 |
Peroxisomes | 0.0025 | 0 | 0.0041 | 0.0019 | 0.002 | 0.0013 | 0.0044 | 0 | 0.0027 | 0.0002 | 0.0003 | 0.0002 | 0.0107 | 0 | 0.0008 | 0 | 0.0018 | 0.0003 |
Punctate Nuclear | 0.0055 | 0.0001 | 0.0006 | 0.0002 | 0.0177 | 0.0005 | 0.0018 | 0.0001 | 0.0008 | 0 | 0.0006 | 0.0005 | 0.0129 | 0 | 0.0021 | 0 | 0.0063 | 0.0025 |
Vacuole | 0.0064 | 0.008 | 0.0039 | 0.0051 | 0.0039 | 0.0021 | 0.0066 | 0.0046 | 0.0033 | 0.0027 | 0.0021 | 0.0031 | 0.003 | 0.0018 | 0.0018 | 0.0078 | 0.001 | 0.0011 |
Vacuole Periphery | 0.0063 | 0.0022 | 0.0038 | 0.0043 | 0.0067 | 0.0018 | 0.0057 | 0.0006 | 0.0007 | 0.0018 | 0.0011 | 0.0023 | 0.0044 | 0.0005 | 0.0018 | 0.0053 | 0.0008 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.226 | 26.9012 | 6.6539 | 15.6207 | 32.5139 | 11.6963 | 20.7776 | 25.4972 | 15.4388 | 27.9091 |
Translational Efficiency | 2.1147 | 1.479 | 2.9466 | 1.356 | 0.9805 | 2.923 | 1.9632 | 1.1005 | 1.7556 | 1.4396 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
70 | 790 | 268 | 1591 | 1640 | 1650 | 2394 | 205 | 1710 | 2440 | 2662 | 1796 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1119.82 | 1390.07 | 1822.00 | 1683.06 | 1091.48 | 1255.21 | 1607.27 | 2053.92 | 1092.64 | 1298.87 | 1628.89 | 1725.39 |
Standard Deviation | 204.50 | 240.67 | 256.62 | 337.20 | 177.76 | 216.84 | 262.90 | 279.97 | 179.02 | 233.52 | 270.11 | 351.53 |
Intensity Change Log 2 | — | 0.311891 | 0.702256 | 0.587820 | — | 0.201643 | 0.558327 | 0.912094 | — | 0.258526 | 0.633007 | 0.756978 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001144 | 0.003850 | 0.000488 | 0.002068 | 0.000813 | 0.003725 | 0.001067 | 0.000483 | 0.000826 | 0.003766 | 0.001009 | 0.001887 |
Bud Neck | 0.001708 | 0.000324 | 0.003690 | 0.003022 | 0.000208 | 0.001146 | 0.000571 | 0.007971 | 0.000270 | 0.000880 | 0.000885 | 0.003587 |
Bud Site | 0.014233 | 0.005939 | 0.002479 | 0.012688 | 0.002978 | 0.008560 | 0.004097 | 0.010010 | 0.003439 | 0.007712 | 0.003934 | 0.012383 |
Cell Periphery | 0.001502 | 0.001243 | 0.001604 | 0.002469 | 0.000743 | 0.001554 | 0.002570 | 0.003994 | 0.000774 | 0.001453 | 0.002472 | 0.002643 |
Cytoplasm | 0.006290 | 0.003977 | 0.003050 | 0.024099 | 0.005388 | 0.028825 | 0.005870 | 0.001495 | 0.005425 | 0.020780 | 0.005586 | 0.021519 |
Cytoplasmic Foci | 0.020809 | 0.006222 | 0.001608 | 0.011844 | 0.004247 | 0.031117 | 0.001717 | 0.001235 | 0.004925 | 0.023057 | 0.001706 | 0.010633 |
Eisosomes | 0.000047 | 0.000063 | 0.000032 | 0.000075 | 0.000038 | 0.000148 | 0.000055 | 0.000056 | 0.000039 | 0.000120 | 0.000052 | 0.000073 |
Endoplasmic Reticulum | 0.837456* | 0.920125* | 0.864430* | 0.742957* | 0.942912* | 0.660160* | 0.933086* | 0.884391* | 0.938595* | 0.744329* | 0.926174* | 0.759101* |
Endosome | 0.021467 | 0.016936 | 0.015904 | 0.030236 | 0.009554 | 0.035354 | 0.011298 | 0.018103 | 0.010041 | 0.029391 | 0.011762 | 0.028851 |
Golgi | 0.010299 | 0.008183 | 0.002184 | 0.013875 | 0.003618 | 0.026462 | 0.005982 | 0.004504 | 0.003891 | 0.020544 | 0.005600 | 0.012806 |
Lipid Particles | 0.012120 | 0.003193 | 0.004811 | 0.032036 | 0.003421 | 0.031590 | 0.006882 | 0.017986 | 0.003777 | 0.022396 | 0.006674 | 0.030433 |
Mitochondria | 0.015184 | 0.001142 | 0.001033 | 0.001663 | 0.001697 | 0.008161 | 0.000574 | 0.000278 | 0.002249 | 0.005889 | 0.000621 | 0.001505 |
Mitotic Spindle | 0.001136 | 0.001741 | 0.000426 | 0.007972 | 0.000101 | 0.003984 | 0.000249 | 0.003154 | 0.000144 | 0.003258 | 0.000266 | 0.007422 |
None | 0.000271 | 0.001661 | 0.000023 | 0.003063 | 0.002248 | 0.009539 | 0.001336 | 0.000329 | 0.002167 | 0.006988 | 0.001204 | 0.002751 |
Nuclear Periphery | 0.000612 | 0.002428 | 0.036025 | 0.010276 | 0.004496 | 0.031502 | 0.001564 | 0.006157 | 0.004337 | 0.022089 | 0.005034 | 0.009806 |
Nuclear Periphery Foci | 0.004606 | 0.013494 | 0.047337 | 0.067117 | 0.013740 | 0.082617 | 0.009895 | 0.018094 | 0.013366 | 0.060237 | 0.013664 | 0.061522 |
Nucleolus | 0.001181 | 0.000068 | 0.000009 | 0.000450 | 0.000030 | 0.000794 | 0.000012 | 0.000024 | 0.000078 | 0.000559 | 0.000012 | 0.000402 |
Nucleus | 0.035906 | 0.000252 | 0.001240 | 0.002728 | 0.000271 | 0.005094 | 0.000214 | 0.000198 | 0.001729 | 0.003526 | 0.000317 | 0.002439 |
Peroxisomes | 0.000920 | 0.000342 | 0.000026 | 0.000294 | 0.000242 | 0.001594 | 0.000326 | 0.000052 | 0.000270 | 0.001189 | 0.000296 | 0.000267 |
Vacuole | 0.010092 | 0.005469 | 0.012432 | 0.027838 | 0.001966 | 0.021507 | 0.010673 | 0.017323 | 0.002299 | 0.016314 | 0.010850 | 0.026638 |
Vacuole Periphery | 0.003017 | 0.003347 | 0.001169 | 0.003226 | 0.001289 | 0.006566 | 0.001961 | 0.004162 | 0.001359 | 0.005524 | 0.001881 | 0.003333 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.64 | 1.25 | -1.70 | 1.01 | -3.95 | -4.16 | -0.62 | 1.01 | 4.61 | 1.30 | -4.14 | -0.49 | -2.97 | 2.30 | -2.02 |
Bud Neck | 1.41 | -1.50 | -1.81 | -9.54 | 0.35 | -6.64 | -3.61 | -3.00 | -2.67 | -2.87 | -5.60 | -4.19 | -8.59 | -7.10 | -6.85 |
Bud Site | 0.88 | 1.25 | 0.05 | -3.59 | -5.93 | -4.64 | -1.39 | -1.90 | -0.58 | -1.59 | -3.91 | -0.72 | -6.24 | -3.36 | -5.88 |
Cell Periphery | 0.45 | -0.19 | -2.02 | -3.70 | -1.55 | -1.62 | -4.98 | -5.11 | -3.15 | -2.04 | -1.90 | -5.05 | -8.09 | -3.16 | -0.57 |
Cytoplasm | 0.77 | 0.87 | -4.43 | -8.71 | -7.69 | -11.35 | -0.76 | 4.16 | 13.49 | 5.38 | -10.05 | -0.42 | -8.03 | -1.05 | -7.96 |
Cytoplasmic Foci | 1.62 | 2.11 | 1.03 | -3.02 | -5.44 | -11.51 | 3.11 | 3.10 | 13.06 | 0.81 | -10.18 | 3.70 | -4.05 | 6.36 | -7.59 |
Eisosomes | -0.27 | 1.05 | -1.08 | -0.52 | -2.45 | -3.30 | -0.72 | -0.72 | 2.44 | -0.06 | -3.22 | -0.64 | -2.26 | 1.94 | -1.12 |
Endoplasmic Reticulum | -2.14 | -0.74 | 2.38 | 17.33 | 8.28 | 30.36* | 2.42 | 3.64 | -10.88 | 2.97 | 25.21 | 2.78 | 21.60 | -0.10 | 20.24 |
Endosome | 0.55 | 0.65 | -1.13 | -7.52 | -6.97 | -15.15 | -2.36 | -3.11 | 4.91 | -2.44 | -14.43 | -2.26 | -13.78 | -0.11 | -12.76 |
Golgi | 0.28 | 1.16 | -0.51 | -2.54 | -6.41 | -10.38 | -2.39 | -0.69 | 9.33 | 1.20 | -9.93 | -1.80 | -5.92 | 3.94 | -4.72 |
Lipid Particles | 0.99 | 0.77 | -2.09 | -11.97 | -9.08 | -11.97 | -3.73 | -2.43 | 1.94 | -1.85 | -10.87 | -3.06 | -11.65 | -3.34 | -10.32 |
Mitochondria | 1.10 | 1.10 | 1.05 | -1.13 | -0.78 | -6.77 | 3.02 | 3.81 | 8.78 | 2.07 | -4.20 | 2.55 | 1.01 | 6.28 | -2.61 |
Mitotic Spindle | -0.44 | 0.81 | -4.78 | -3.91 | -5.88 | -4.81 | -1.58 | -1.94 | 0.52 | -1.84 | -4.70 | -1.36 | -6.36 | -3.42 | -6.24 |
None | -2.05 | 2.00 | -3.26 | -1.32 | -3.63 | -4.41 | 1.45 | 3.53 | 5.80 | 2.63 | -4.05 | 1.63 | -0.65 | 3.24 | -1.90 |
Nuclear Periphery | -4.58 | -6.94 | -8.55 | -6.74 | 4.91 | -11.28 | 5.12 | -0.69 | 7.08 | -1.78 | -10.53 | -0.99 | -4.82 | 6.30 | -4.07 |
Nuclear Periphery Foci | -3.38 | -6.83 | -15.48 | -13.02 | -3.03 | -16.01* | 2.06 | -1.00 | 9.54 | -1.65 | -14.74 | -0.47 | -13.54 | -0.88 | -13.60 |
Nucleolus | 1.90 | 1.99 | 1.24 | -2.04 | -2.40 | -4.04 | 2.33 | 0.34 | 4.06 | -0.85 | -3.64 | 2.48 | -1.95 | 0.87 | -2.40 |
Nucleus | 1.86 | 1.80 | 1.71 | -4.99 | -1.35 | -6.11 | 0.59 | -0.15 | 6.04 | -0.44 | -1.83 | 1.71 | -1.09 | 1.01 | -4.74 |
Peroxisomes | 1.66 | 2.57 | 1.89 | 0.20 | -3.33 | -6.47 | -0.53 | 3.85 | 7.43 | 1.81 | -5.79 | -0.11 | 0.08 | 5.51 | 0.15 |
Vacuole | 1.21 | 0.03 | -2.96 | -10.17 | -5.27 | -10.23 | -9.20 | -3.46 | 0.39 | -1.61 | -10.08 | -9.10 | -12.80 | -5.58 | -8.17 |
Vacuole Periphery | -0.23 | 1.26 | -0.17 | 0.14 | -4.32 | -7.92 | -2.31 | -1.45 | 1.13 | -1.08 | -7.75 | -1.93 | -4.35 | 3.38 | -3.04 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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