Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding by the complex; required for tRNA genes clustering at the nucleolus; required for replication slow zone breakage following Mec1p inactivation; transcription is cell cycle regulated, peaking in mitosis and declining in G1; protein is constitutively degraded by the proteasome; rate limiting for condensin recruitment to chromatin |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 4 | 5 | – | 2 | 3 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 2 | 0 | 2 | 6 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 3 | 4 | 8 | 6 | – | 8 | 15 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 0 | 6 | 7 | 11 | 7 | 12 | 8 | – | 6 | 9 | 32 | 53 | 78 | 15 | 9 | 18 | 0 | 0 | 0 | 2 | 2 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 2 | 0 | 0 | 0 | 1 | 1 | 0 |
Endosome | 12 | 3 | 0 | 0 | 9 | 2 | 4 | 0 | 0 | – | 0 | 1 | 2 | 20 | 13 | 16 | 18 | 22 | 22 | 9 | 5 | 33 | 59 | 89 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 2 |
Mitochondria | 15 | 24 | 14 | 52 | 62 | 83 | 136 | 173 | 148 | – | 98 | 182 | 13 | 17 | 115 | 12 | 6 | 22 | 3 | 2 | 2 | 2 | 4 | 13 |
Nucleus | 145 | 159 | 8 | 34 | 57 | 57 | 97 | 133 | 100 | – | 60 | 82 | 207 | 222 | 221 | 128 | 79 | 79 | 83 | 145 | 24 | 69 | 77 | 83 |
Nuclear Periphery | 7 | 10 | 0 | 2 | 2 | 1 | 3 | 6 | 5 | – | 1 | 7 | 6 | 9 | 7 | 9 | 10 | 6 | 14 | 2 | 3 | 2 | 2 | 7 |
Nucleolus | 73 | 38 | 3 | 18 | 50 | 45 | 47 | 61 | 51 | – | 19 | 43 | 38 | 85 | 121 | 54 | 54 | 82 | 74 | 48 | 12 | 66 | 55 | 76 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 19 | 3 | 0 | 0 | 30 | 16 | 17 | 29 | 19 | – | 7 | 8 | 0 | 7 | 14 | 6 | 10 | 16 | 34 | 13 | 4 | 39 | 58 | 88 |
Vac/Vac Membrane | 6 | 11 | 0 | 0 | 0 | 2 | 5 | 2 | 0 | – | 2 | 5 | 4 | 9 | 13 | 17 | 9 | 14 | 9 | 14 | 7 | 16 | 43 | 58 |
Unique Cell Count | 229 | 217 | 19 | 71 | 155 | 147 | 219 | 297 | 211 | 132 | 220 | 277 | 376 | 475 | 214 | 161 | 204 | 259 | 243 | 64 | 248 | 328 | 450 | |
Labelled Cell Count | 280 | 253 | 25 | 115 | 220 | 223 | 323 | 429 | 342 | 204 | 355 | 302 | 423 | 584 | 261 | 199 | 266 | 259 | 243 | 64 | 248 | 328 | 450 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 5.2 | 3.7 | 4.0 | 4.2 | 3.8 | 3.6 | 3.7 | 3.2 | – | 3.3 | 2.8 | 5.6 | 5.7 | 4.8 | 8.1 | 9.0 | 9.4 | 5.7 | 5.1 | 5.9 |
Std Deviation (1e-4) | 0.9 | 0.8 | 0.3 | 1.2 | 1.4 | 1.3 | 1.1 | 1.2 | 1.2 | – | 1.2 | 0.7 | 1.3 | 1.4 | 1.3 | 2.3 | 2.4 | 2.9 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.15 | 0.01 | -0.07 | -0.02 | -0.23 | – | -0.18 | -0.4 | 0.59 | 0.62 | 0.36 | 1.11 | 1.27 | 1.33 | 0.6 | 0.45 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.4 | -0.5 | -0.3 | 0.2 | 0.2 | 0.4 | – | 0.3 | -0.4 | 3.1 | 1.5 | 0.4 | 1.5 | 0.6 | -0.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.019 | 1.6757 | 1.8376 | 1.6545 | 1.5313 | 1.4015 | 3.2021 | 2.5995 | 2.6106 | 2.1116 | 2.0271 | 2.3309 | 0.7623 | 1.0011 | 0.8362 | 0.655 | 0.474 | 0.7083 |
Actin | 0.0464 | 0.0005 | 0.0119 | 0.0021 | 0.0465 | 0.0005 | 0.0403 | 0.0002 | 0.0227 | 0.0026 | 0.0007 | 0.0046 | 0.0222 | 0.0004 | 0.0063 | 0.0008 | 0.0004 | 0.0003 |
Bud | 0.0008 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0037 | 0.0026 | 0.0054 | 0.0132 | 0.0604 | 0.0052 | 0.0031 | 0.0014 | 0.0027 | 0.0014 | 0.0068 | 0.0035 | 0.0014 | 0.0008 | 0.007 | 0.0019 | 0.047 | 0.0047 |
Bud Periphery | 0.0019 | 0.0011 | 0.0011 | 0.0012 | 0.0007 | 0.0003 | 0.0027 | 0.0003 | 0.0014 | 0.0002 | 0.0004 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0002 |
Bud Site | 0.0157 | 0.0055 | 0.0115 | 0.0019 | 0.0011 | 0.0004 | 0.0046 | 0.002 | 0.0048 | 0.0006 | 0.0005 | 0.0005 | 0.0011 | 0.0009 | 0.0022 | 0.0004 | 0.0015 | 0.0004 |
Cell Periphery | 0.0007 | 0.0007 | 0.002 | 0.0007 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0023 | 0.0101 | 0.0029 | 0.0002 | 0.0004 | 0.0011 | 0.0086 | 0.0046 | 0.002 | 0.001 | 0.0002 | 0.0009 | 0.0044 | 0.0048 | 0.0016 | 0.0005 | 0.0003 | 0.0011 |
Cytoplasmic Foci | 0.0109 | 0.0026 | 0.0062 | 0.0028 | 0.0104 | 0.0003 | 0.0119 | 0.0017 | 0.0088 | 0.0042 | 0.0033 | 0.002 | 0.0021 | 0.0006 | 0.0028 | 0.0012 | 0.0071 | 0.0006 |
Eisosomes | 0.0015 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0017 | 0.0014 | 0.0005 | 0.0011 | 0.0008 | 0.005 | 0.0004 | 0.0018 | 0.0014 | 0.0001 | 0.0004 | 0.0078 | 0.0023 | 0.0005 | 0.0002 | 0.0001 | 0.0003 |
Endosome | 0.0189 | 0.0054 | 0.007 | 0.0054 | 0.0059 | 0.0009 | 0.0355 | 0.0018 | 0.0149 | 0.0099 | 0.0072 | 0.0046 | 0.0101 | 0.0029 | 0.0017 | 0.0007 | 0.0016 | 0.0007 |
Golgi | 0.008 | 0.0007 | 0.0035 | 0.0025 | 0.0066 | 0.0002 | 0.0123 | 0.0003 | 0.0043 | 0.0064 | 0.0066 | 0.0025 | 0.002 | 0.0003 | 0.0008 | 0.0003 | 0.0013 | 0.0002 |
Lipid Particles | 0.0267 | 0.0054 | 0.0213 | 0.0169 | 0.021 | 0.0011 | 0.0247 | 0.0018 | 0.0189 | 0.0139 | 0.0148 | 0.0021 | 0.006 | 0.0033 | 0.0042 | 0.0036 | 0.0196 | 0.0018 |
Mitochondria | 0.0055 | 0.0021 | 0.0021 | 0.0031 | 0.0013 | 0.0011 | 0.0398 | 0.0005 | 0.0053 | 0.0127 | 0.0249 | 0.0022 | 0.0024 | 0.0009 | 0.0006 | 0.0005 | 0.0006 | 0.0005 |
None | 0.0056 | 0.0128 | 0.0018 | 0.0004 | 0.0006 | 0.0003 | 0.0099 | 0.0006 | 0.0006 | 0.0015 | 0.0004 | 0.0007 | 0.0192 | 0.0639 | 0.0026 | 0.0006 | 0.0004 | 0.0009 |
Nuclear Periphery | 0.047 | 0.06 | 0.0514 | 0.0049 | 0.0057 | 0.045 | 0.067 | 0.0301 | 0.0548 | 0.0115 | 0.0115 | 0.0151 | 0.0171 | 0.0883 | 0.0678 | 0.0631 | 0.0031 | 0.0337 |
Nucleolus | 0.1337 | 0.1843 | 0.3322 | 0.4826 | 0.2818 | 0.2096 | 0.1185 | 0.2074 | 0.2963 | 0.3929 | 0.2904 | 0.223 | 0.1727 | 0.1033 | 0.3512 | 0.4835 | 0.3705 | 0.1769 |
Nucleus | 0.6159 | 0.5831 | 0.3799 | 0.1714 | 0.161 | 0.6124 | 0.4972 | 0.6671 | 0.4377 | 0.285 | 0.2239 | 0.6136 | 0.5917 | 0.5369 | 0.4023 | 0.2703 | 0.1464 | 0.6144 |
Peroxisomes | 0.0069 | 0.0006 | 0.0029 | 0.002 | 0.0265 | 0.0001 | 0.0085 | 0.0006 | 0.004 | 0.0054 | 0.0015 | 0.0011 | 0.0033 | 0.0002 | 0.0058 | 0.0006 | 0.0038 | 0.0002 |
Punctate Nuclear | 0.0353 | 0.1063 | 0.1447 | 0.2836 | 0.3674 | 0.1187 | 0.0965 | 0.0765 | 0.1145 | 0.2473 | 0.4051 | 0.1213 | 0.1302 | 0.1844 | 0.139 | 0.1699 | 0.3943 | 0.1617 |
Vacuole | 0.0057 | 0.0112 | 0.0068 | 0.0031 | 0.0006 | 0.0009 | 0.0065 | 0.0017 | 0.0015 | 0.0009 | 0.0005 | 0.0007 | 0.0039 | 0.0027 | 0.0022 | 0.0003 | 0.0008 | 0.0007 |
Vacuole Periphery | 0.003 | 0.003 | 0.0035 | 0.0008 | 0.0003 | 0.0009 | 0.0052 | 0.0007 | 0.0015 | 0.0009 | 0.0008 | 0.0004 | 0.001 | 0.0026 | 0.0007 | 0.0007 | 0.0001 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.9936 | 17.1378 | 8.478 | 11.0199 | 14.7296 | 19.8702 | 24.9491 | 17.1097 | 13.2836 | 17.4591 |
Translational Efficiency | 0.5842 | 0.8344 | 0.692 | 0.5137 | 0.551 | 0.4106 | 0.4853 | 0.3937 | 0.5015 | 0.4689 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
338 | 738 | 1028 | 419 | 1564 | 1640 | 951 | 615 | 1902 | 2378 | 1979 | 1034 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 662.41 | 780.14 | 1014.22 | 941.02 | 676.36 | 757.73 | 929.48 | 981.60 | 673.88 | 764.68 | 973.50 | 965.16 |
Standard Deviation | 75.07 | 126.41 | 128.55 | 137.25 | 93.73 | 102.51 | 118.44 | 136.18 | 90.85 | 110.97 | 130.83 | 138.06 |
Intensity Change Log 2 | — | 0.236009 | 0.614574 | 0.506501 | — | 0.163893 | 0.458632 | 0.537344 | — | 0.200025 | 0.537897 | 0.522166 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001045 | 0.000893 | 0.001236 | 0.001087 | 0.000411 | 0.000597 | 0.001311 | 0.001537 | 0.000524 | 0.000689 | 0.001272 | 0.001355 |
Bud Neck | 0.002958 | 0.004553 | 0.024333 | 0.024417 | 0.006685 | 0.007498 | 0.011031 | 0.018999 | 0.006023 | 0.006584 | 0.017940 | 0.021195 |
Bud Site | 0.000928 | 0.001408 | 0.003656 | 0.011801 | 0.001142 | 0.001558 | 0.002619 | 0.005646 | 0.001104 | 0.001511 | 0.003158 | 0.008140 |
Cell Periphery | 0.000084 | 0.000120 | 0.000065 | 0.000191 | 0.000112 | 0.000108 | 0.000076 | 0.000097 | 0.000107 | 0.000112 | 0.000071 | 0.000135 |
Cytoplasm | 0.023920 | 0.020836 | 0.002445 | 0.024456 | 0.023857 | 0.011908 | 0.002978 | 0.003238 | 0.023868 | 0.014679 | 0.002701 | 0.011836 |
Cytoplasmic Foci | 0.011809 | 0.007223 | 0.000138 | 0.005430 | 0.004877 | 0.006879 | 0.001229 | 0.001722 | 0.006109 | 0.006986 | 0.000663 | 0.003225 |
Eisosomes | 0.000067 | 0.000066 | 0.000038 | 0.000051 | 0.000050 | 0.000057 | 0.000053 | 0.000047 | 0.000053 | 0.000060 | 0.000045 | 0.000048 |
Endoplasmic Reticulum | 0.001890 | 0.005102 | 0.005954 | 0.004399 | 0.002299 | 0.004501 | 0.004552 | 0.007592 | 0.002226 | 0.004688 | 0.005280 | 0.006298 |
Endosome | 0.001588 | 0.002378 | 0.000642 | 0.020182 | 0.001941 | 0.002860 | 0.001063 | 0.005351 | 0.001878 | 0.002710 | 0.000845 | 0.011361 |
Golgi | 0.001332 | 0.001512 | 0.000370 | 0.004685 | 0.001200 | 0.002822 | 0.000771 | 0.002809 | 0.001224 | 0.002415 | 0.000563 | 0.003569 |
Lipid Particles | 0.007845 | 0.004503 | 0.000341 | 0.001733 | 0.002339 | 0.006971 | 0.000758 | 0.000924 | 0.003318 | 0.006205 | 0.000541 | 0.001252 |
Mitochondria | 0.007013 | 0.005672 | 0.002012 | 0.022641 | 0.003876 | 0.011935 | 0.001598 | 0.009427 | 0.004433 | 0.009991 | 0.001813 | 0.014781 |
Mitotic Spindle | 0.023539 | 0.045869 | 0.049960 | 0.396583* | 0.020981 | 0.041581 | 0.057595 | 0.272474* | 0.021435 | 0.042912 | 0.053629 | 0.322766* |
None | 0.015530 | 0.020911 | 0.002719 | 0.009944 | 0.018552 | 0.006342 | 0.004008 | 0.003016 | 0.018015 | 0.010864 | 0.003338 | 0.005824 |
Nuclear Periphery | 0.002859 | 0.003004 | 0.001973 | 0.006810 | 0.003205 | 0.009409 | 0.001274 | 0.006874 | 0.003144 | 0.007422 | 0.001637 | 0.006848 |
Nuclear Periphery Foci | 0.024472 | 0.043034 | 0.009729 | 0.015810 | 0.034064 | 0.029022 | 0.010870 | 0.030142 | 0.032359 | 0.033370 | 0.010277 | 0.024335 |
Nucleolus | 0.359499* | 0.261886* | 0.154739 | 0.141957 | 0.363327* | 0.279280* | 0.352813* | 0.248058* | 0.362647* | 0.273882* | 0.249922* | 0.205063 |
Nucleus | 0.506876* | 0.564089* | 0.734890* | 0.273543* | 0.507677* | 0.568521* | 0.541790* | 0.365292* | 0.507534* | 0.567145* | 0.642097* | 0.328113* |
Peroxisomes | 0.005490 | 0.004744 | 0.001212 | 0.004251 | 0.002203 | 0.004387 | 0.001943 | 0.001563 | 0.002787 | 0.004498 | 0.001563 | 0.002652 |
Vacuole | 0.000889 | 0.001642 | 0.003245 | 0.022417 | 0.000840 | 0.002801 | 0.001342 | 0.010666 | 0.000849 | 0.002442 | 0.002330 | 0.015428 |
Vacuole Periphery | 0.000368 | 0.000554 | 0.000303 | 0.007613 | 0.000363 | 0.000962 | 0.000327 | 0.004525 | 0.000364 | 0.000835 | 0.000315 | 0.005776 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.19 | -1.37 | -1.76 | -5.95 | -1.61 | -3.22 | -6.95 | -12.91 | -11.15 | -3.35 | -2.05 | -7.95 | -10.76 | -12.83 | -3.77 |
Bud Neck | -3.48 | -16.78 | -10.36 | -9.71 | -0.43 | -1.44 | -6.99 | -12.66 | -12.26 | -9.07 | -1.58 | -16.16 | -15.81 | -15.46 | -5.38 |
Bud Site | -2.25 | -5.06 | -8.30 | -6.98 | -4.78 | -1.33 | -3.42 | -8.70 | -8.15 | -4.66 | -2.23 | -5.84 | -11.88 | -10.83 | -6.57 |
Cell Periphery | -2.76 | -0.34 | -4.29 | -1.64 | -4.86 | 0.16 | 2.13 | -0.13 | -0.28 | -3.10 | -0.45 | 2.08 | -1.99 | -1.17 | -5.74 |
Cytoplasm | 0.43 | 4.00 | 0.53 | 0.14 | -6.27 | 4.28 | 9.09 | 7.50 | 4.48 | -2.96 | 3.53 | 10.13 | 5.03 | 2.04 | -6.92 |
Cytoplasmic Foci | 1.16 | 4.44 | 3.57 | 4.02 | -3.67 | -1.63 | 5.70 | 4.18 | 5.14 | -0.59 | -0.99 | 8.04 | 5.61 | 6.54 | -2.27 |
Eisosomes | -0.32 | 4.65 | -0.15 | 0.12 | -4.43 | -2.56 | -2.15 | -2.10 | 0.06 | -0.32 | -3.09 | 1.73 | -2.60 | -0.19 | -4.00 |
Endoplasmic Reticulum | -6.48 | -10.58 | -9.81 | -5.56 | -3.92 | -7.66 | -9.83 | -10.56 | -7.25 | -6.42 | -10.04 | -15.15 | -14.34 | -9.27 | -7.17 |
Endosome | -1.11 | 2.90 | -3.63 | -3.07 | -4.13 | -1.13 | 2.83 | -1.74 | -0.61 | -4.08 | -1.34 | 3.84 | -3.81 | -3.00 | -5.39 |
Golgi | -0.24 | 2.72 | -0.15 | 0.02 | -2.20 | -2.04 | 2.63 | 0.34 | 2.01 | -1.21 | -2.07 | 4.31 | 0.01 | 1.78 | -2.52 |
Lipid Particles | 1.50 | 3.84 | 3.26 | 4.01 | -5.19 | -4.60 | 4.88 | 5.66 | 6.42 | -0.81 | -3.60 | 6.83 | 5.55 | 7.30 | -3.52 |
Mitochondria | 0.22 | 1.32 | -0.71 | -1.10 | -2.17 | -6.56 | 5.09 | -1.17 | 3.53 | -2.80 | -5.27 | 3.58 | -1.70 | 1.69 | -3.30 |
Mitotic Spindle | -3.07 | -4.26 | -14.17 | -12.71 | -12.32 | -4.09 | -7.20 | -15.13 | -13.47 | -10.75 | -5.22 | -8.50 | -20.86 | -18.59 | -16.35 |
None | -1.38 | 4.76 | 2.52 | 4.14 | -4.02 | 8.74 | 10.31 | 10.91 | 4.75 | -0.42 | 4.95 | 12.18 | 9.71 | 4.99 | -3.71 |
Nuclear Periphery | -0.38 | 0.23 | -5.01 | -5.40 | -5.97 | -7.41 | 4.80 | -5.73 | 1.64 | -8.40 | -7.02 | 3.27 | -7.59 | -0.65 | -10.00 |
Nuclear Periphery Foci | -2.90 | 4.17 | 2.74 | 6.58 | -2.50 | 2.24 | 7.68 | 4.40 | 2.51 | -2.22 | 0.44 | 9.90 | 6.04 | 5.96 | -3.46 |
Nucleolus | 5.92 | 10.42 | 13.00 | 10.29 | 4.95 | 8.73 | 1.31 | 12.82 | 5.91 | 10.70 | 10.75 | 11.23 | 19.57 | 11.05 | 9.64 |
Nucleus | -3.09 | -9.64 | 2.73 | 6.51 | 13.62 | -6.11 | -1.94 | 3.93 | 8.50 | 5.18 | -6.74 | -10.59 | 5.18 | 10.85 | 13.94 |
Peroxisomes | -0.05 | 2.70 | 2.18 | 3.19 | -1.27 | -2.83 | 1.01 | 2.82 | 4.71 | 3.04 | -2.77 | 3.15 | 2.99 | 5.46 | 0.24 |
Vacuole | -1.25 | -5.28 | -7.21 | -7.09 | -6.34 | -4.51 | -4.18 | -6.95 | -5.82 | -6.10 | -4.81 | -7.24 | -9.72 | -8.95 | -8.36 |
Vacuole Periphery | -1.33 | -0.87 | -2.14 | -1.94 | -2.09 | -3.35 | -0.59 | -2.13 | -1.22 | -2.09 | -3.59 | -1.14 | -3.00 | -2.20 | -2.94 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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