ORF
Human Ortholog
Description Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.32 0.29 0.6 0.6 0.73 – 0.72 0.78 0.72 0.73 0.69 0.32 0.32 0.4 – 0.5 0.37 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.06 0 – 0 0.05 0 0 0 0 0 0
Endosome 0.09 0 0.16 0.19 0.15 0 – 0 0 0 0 0 0 0 0.05 – 0 0.06 0.07 0.09 0.11 0.07 0.11 0.1
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.25 0.12 0.11 0.06 0.19 – 0.28 0.29 0.39 0.38 0.38 0 0 0 – 0.09 0 0 0.05 0 0 0 0
Nucleus 0.05 0.05 0 0 0 0 – 0 0 0 0 0 0.17 0.17 0.08 – 0.07 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.76 0.58 0.7 0.34 0.4 0.25 – 0.17 0.13 0.16 0.14 0.16 0.56 0.57 0.62 – 0.45 0.61 0.82 0.77 0.75 0.8 0.75 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 – 0 0 0 1 0 0 1 2
Bud 0 1 1 0 0 4 – 11 5 12 20 11 0 0 0 – 0 0 0 1 1 3 3 6
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 2 2 0 – 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 – 2 4 2 1 5 1 1 0 – 0 0 0 0 0 0 0 0
Cytoplasm 61 110 88 149 227 340 – 558 391 462 373 457 81 81 122 – 72 87 2 14 4 6 17 14
Endoplasmic Reticulum 4 7 0 2 2 2 – 6 0 3 2 5 16 14 1 – 6 11 0 1 2 3 4 9
Endosome 23 0 47 47 55 20 – 28 7 6 6 9 4 1 14 – 2 14 19 40 36 19 81 65
Golgi 0 1 1 1 0 0 – 0 0 0 0 0 0 0 0 – 0 0 2 1 1 0 3 3
Mitochondria 6 86 35 28 23 88 – 220 148 253 192 250 0 0 2 – 13 7 8 22 11 10 14 22
Nucleus 12 17 1 2 3 1 – 4 2 0 2 9 42 42 24 – 10 14 4 4 0 5 11 6
Nuclear Periphery 1 5 4 0 0 0 – 5 2 3 0 3 0 0 0 – 1 0 5 1 19 3 30 24
Nucleolus 0 0 1 0 0 0 – 0 0 0 2 0 0 0 0 – 0 1 2 0 0 0 0 0
Peroxisomes 0 0 1 0 1 0 – 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 2 3 2 4 0 – 1 3 4 1 2 2 2 1 – 0 1 1 1 1 0 3 4
Vac/Vac Membrane 187 199 210 85 152 116 – 132 67 100 72 109 143 144 187 – 65 143 223 330 245 216 544 485
Unique Cell Count 245 344 302 249 377 467 778 503 644 510 666 254 251 303 144 236 273 427 328 271 724 656
Labelled Cell Count 295 428 392 316 467 572 967 629 845 671 860 291 288 351 169 278 273 427 328 271 724 656


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.2 4.9 5.1 5.0 4.2 – 4.2 4.1 3.8 4.1 4.1 5.8 5.8 6.0 4.5 5.2 5.9 6.3 6.4 6.6
Std Deviation (1e-4) 0.8 0.6 1.1 1.8 1.8 1.4 – 1.1 0.9 0.8 1.0 0.8 0.9 0.9 0.8 0.9 1.1 1.2 1.2 1.2 1.5
Intensity Change (Log2) – – – 0.08 0.04 -0.21 – -0.2 -0.24 -0.35 -0.27 -0.26 0.25 0.25 0.31 -0.11 0.1 0.29 0.37 0.39 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – -5.0 -2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1189 0.7734 0.3959 0.1075 0.0202 0.4398 1.7342 1.8876 1.7859 1.6295 1.4351 1.8567 0.9314 1.7171 1.4097 1.2864 1.1574 1.4971
Actin 0.0102 0.0001 0.0002 0.0002 0.0003 0.0002 0.0268 0.0002 0.0001 0.013 0.025 0.0025 0.0092 0 0.0002 0.0004 0.0001 0.0002
Bud 0.0002 0.0109 0.0008 0.0075 0.0008 0.0003 0.0005 0.0013 0.0017 0.0038 0.0005 0.0004 0.0003 0.0035 0.001 0.0008 0.0003 0.0004
Bud Neck 0.0014 0.0002 0.0005 0.0006 0.0005 0.0016 0.0008 0.0003 0.0008 0.0001 0.0003 0.001 0.0006 0.0004 0.0009 0.0006 0.0003 0.0012
Bud Periphery 0.0004 0.0019 0.0007 0.0017 0.0031 0.0005 0.0008 0.0012 0.0025 0.0305 0.0007 0.0009 0.0007 0.0027 0.0013 0.0011 0.0002 0.0005
Bud Site 0.001 0.0028 0.0022 0.0075 0.0006 0.0008 0.0031 0.0026 0.0017 0.0005 0.0007 0.0006 0.0008 0.0042 0.0065 0.001 0.0003 0.0004
Cell Periphery 0.0005 0.0005 0.0003 0.0014 0.0003 0.0002 0.001 0.0006 0.0005 0.0004 0.0001 0.0006 0.0005 0.0004 0.0004 0.0003 0.0001 0.0002
Cytoplasm 0.137 0.1749 0.165 0.0922 0.1232 0.2123 0.0951 0.2635 0.1829 0.0712 0.0687 0.1759 0.1872 0.2837 0.1905 0.2767 0.2396 0.2837
Cytoplasmic Foci 0.0607 0.055 0.0562 0.0474 0.067 0.0678 0.0536 0.0674 0.0448 0.0228 0.0386 0.0516 0.0506 0.0357 0.0612 0.0645 0.0746 0.0699
Eisosomes 0.0004 0 0.0001 0.0001 0.0001 0 0.0004 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.007 0.0128 0.0059 0.0041 0.0035 0.0048 0.0099 0.0056 0.0053 0.0032 0.0032 0.0047 0.0055 0.0075 0.0078 0.0052 0.0026 0.0053
Endosome 0.2863 0.2374 0.2599 0.3236 0.2696 0.2726 0.2318 0.2008 0.1992 0.1997 0.3531 0.2165 0.2712 0.161 0.3188 0.2994 0.3362 0.2418
Golgi 0.0037 0.0026 0.0029 0.0028 0.0029 0.0034 0.0087 0.0031 0.0029 0.0022 0.0071 0.0031 0.0025 0.0016 0.0041 0.0034 0.0029 0.0022
Lipid Particles 0.0093 0.0071 0.0057 0.0058 0.0045 0.0075 0.0236 0.0052 0.0084 0.0026 0.0046 0.0106 0.0086 0.0049 0.005 0.0049 0.0061 0.0032
Mitochondria 0.0061 0.0028 0.005 0.0058 0.0046 0.0069 0.0192 0.005 0.0066 0.0076 0.0045 0.0034 0.0026 0.0022 0.0052 0.0054 0.002 0.0033
None 0.2424 0.3044 0.2598 0.0901 0.2572 0.1815 0.2983 0.2615 0.3198 0.4217 0.3207 0.3187 0.2188 0.2855 0.1606 0.1508 0.1454 0.2056
Nuclear Periphery 0.0081 0.0113 0.008 0.0053 0.0039 0.0057 0.029 0.0036 0.0056 0.0033 0.0081 0.0094 0.0085 0.0106 0.0135 0.0047 0.0023 0.0032
Nucleolus 0.0015 0.0015 0.0013 0.0029 0.0014 0.0014 0.0021 0.0012 0.0023 0.0006 0.0003 0.0011 0.0012 0.0014 0.0013 0.0012 0.0002 0.0006
Nucleus 0.0063 0.0055 0.0048 0.0049 0.0038 0.0073 0.0065 0.0038 0.0035 0.0021 0.0013 0.0035 0.0053 0.006 0.0049 0.0054 0.0032 0.0037
Peroxisomes 0.0009 0.0006 0.0013 0.0007 0.0009 0.0015 0.0086 0.0011 0.002 0.0004 0.0015 0.0124 0.0005 0.0003 0.0011 0.0016 0.0009 0.0005
Punctate Nuclear 0.0031 0.0017 0.0015 0.0013 0.0015 0.0017 0.0067 0.0013 0.0006 0.0005 0.0022 0.0047 0.0011 0.0013 0.0022 0.0016 0.0004 0.0009
Vacuole 0.1827 0.1452 0.1846 0.2951 0.1974 0.1801 0.151 0.1567 0.1741 0.1654 0.1303 0.1521 0.2031 0.1571 0.1826 0.1442 0.1477 0.1471
Vacuole Periphery 0.0309 0.0207 0.0332 0.0991 0.0531 0.0417 0.0225 0.014 0.0347 0.0481 0.0285 0.0263 0.0211 0.0299 0.0311 0.0268 0.0346 0.026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3414 6.6136 8.7636 10.1837 5.4813 9.5068 8.5976 8.8654 9.5919 11.3504
Translational Efficiency 0.8694 0.5632 0.4165 0.4339 0.7817 0.5096 0.586 0.5391 0.6139 0.4778

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
163 1220 216 1302 1752 1606 860 1558 1915 2826 1076 2860

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 913.09 758.57 960.31 855.01 690.02 783.63 919.64 872.14 709.01 772.81 927.80 864.34
Standard Deviation 131.81 114.80 93.44 127.41 81.22 94.89 104.10 116.63 106.72 104.69 103.34 121.95
Intensity Change Log 2 — -0.267475 0.072743 -0.094816 — 0.183534 0.414431 0.337922 — -0.055884 0.229821 0.107520

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000950 0.000098 0.000630 0.000407 0.000040 0.000096 0.000148 0.000532 0.000118 0.000097 0.000245 0.000475
Bud Neck 0.007442 0.011946 0.015757 0.020856 0.010881 0.013327 0.022868 0.015097 0.010588 0.012731 0.021440 0.017719
Bud Site 0.017965 0.011498 0.009460 0.033016 0.004891 0.007111 0.008324 0.020287 0.006004 0.009005 0.008552 0.026082
Cell Periphery 0.000107 0.000074 0.000061 0.000196 0.000084 0.000132 0.000057 0.000050 0.000086 0.000107 0.000058 0.000116
Cytoplasm 0.091401 0.187514* 0.086098 0.180862* 0.214353* 0.050678 0.082375 0.212150* 0.203888* 0.109751* 0.083122 0.197906*
Cytoplasmic Foci 0.046905 0.119994 0.024740 0.028957 0.137491 0.079196 0.010479 0.029791 0.129781 0.096809 0.013342 0.029412
Eisosomes 0.000046 0.000032 0.000040 0.000036 0.000050 0.000043 0.000029 0.000016 0.000050 0.000038 0.000031 0.000025
Endoplasmic Reticulum 0.003996 0.004080 0.007401 0.003005 0.004473 0.007359 0.007139 0.001979 0.004432 0.005943 0.007191 0.002446
Endosome 0.089562 0.100354 0.053399 0.043847 0.088048 0.136799 0.035685 0.047851 0.088177 0.121065 0.039241 0.046028
Golgi 0.007640 0.002840 0.001197 0.003004 0.001659 0.005686 0.000730 0.003265 0.002168 0.004457 0.000824 0.003146
Lipid Particles 0.003273 0.005240 0.003150 0.002755 0.009455 0.014033 0.002009 0.001424 0.008928 0.010237 0.002238 0.002030
Mitochondria 0.012707 0.003630 0.001441 0.003726 0.006278 0.014036 0.001771 0.002628 0.006825 0.009544 0.001705 0.003128
Mitotic Spindle 0.004881 0.009014 0.012668 0.012901 0.000865 0.002460 0.003088 0.011335 0.001207 0.005290 0.005011 0.012048
None 0.000174 0.002320 0.000419 0.001784 0.000676 0.000989 0.000656 0.001242 0.000633 0.001564 0.000609 0.001489
Nuclear Periphery 0.001150 0.001447 0.007208 0.002285 0.001452 0.002058 0.006645 0.001936 0.001426 0.001794 0.006758 0.002095
Nuclear Periphery Foci 0.001399 0.001212 0.005108 0.001300 0.000401 0.000663 0.001346 0.000905 0.000486 0.000900 0.002102 0.001085
Nucleolus 0.001833 0.002028 0.009928 0.003414 0.001443 0.001822 0.013854 0.002521 0.001476 0.001911 0.013066 0.002928
Nucleus 0.057188 0.024153 0.173623* 0.073367 0.035817 0.018194 0.167857* 0.042322 0.037636 0.020766 0.169015* 0.056455
Peroxisomes 0.001897 0.003078 0.003980 0.002816 0.001633 0.003905 0.001207 0.001333 0.001656 0.003548 0.001763 0.002008
Vacuole 0.635654* 0.465415* 0.517836* 0.565393* 0.458840* 0.559247* 0.605847* 0.579330* 0.473890* 0.518739* 0.588179* 0.572985*
Vacuole Periphery 0.013828 0.044032 0.065859 0.016072 0.021170 0.082165 0.027886 0.024008 0.020545 0.065703 0.035509 0.020395

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.44 -0.18 0.94 -3.22 1.04 -2.10 -2.41 -1.91 -1.62 -1.32 0.21 -1.60 -2.30 -2.51 -0.45
Bud Neck -2.96 -3.95 -6.42 -4.30 -1.02 -1.82 -7.27 -3.56 -1.25 4.54 -2.16 -7.78 -6.16 -3.78 3.43
Bud Site 1.15 1.39 -1.79 -5.37 -5.75 -2.03 -2.37 -7.54 -5.81 -4.39 -2.98 -2.16 -9.80 -7.86 -7.00
Cell Periphery 1.11 1.36 -0.93 -1.50 -1.62 -1.92 1.16 1.61 8.31 1.08 -1.00 1.21 -0.25 0.62 -1.50
Cytoplasm -5.78 0.23 -7.13 -2.15 -9.42 26.94 15.03 -2.75 -26.12 -16.24 14.83 15.09 -3.02 -19.21 -18.86
Cytoplasmic Foci -11.76 3.99 3.71 21.84 -1.05 12.69 37.30 30.74 15.44 -11.27 7.90 36.22 31.49 26.09 -11.07
Eisosomes 1.93 0.20 2.20 0.62 2.46 2.13 7.76 14.00 10.62 6.30 4.09 6.91 11.03 8.00 4.13
Endoplasmic Reticulum -0.23 -2.53 0.64 3.27 8.00 -4.81 -3.98 6.48 11.48 10.24 -3.18 -4.88 5.48 11.28 11.55
Endosome -1.70 2.80 4.43 15.54 2.51 -10.42 17.40 15.14 22.28 -2.34 -8.96 16.51 17.51 27.05 -0.42
Golgi 1.26 1.85 1.46 0.77 -1.23 -4.79 3.16 -1.08 3.15 -2.55 -3.96 3.49 -0.36 3.08 -2.93
Lipid Particles -3.02 -0.26 0.94 4.46 1.28 -3.51 11.50 12.53 10.56 2.60 -1.58 11.05 11.27 11.31 1.08
Mitochondria 1.15 1.49 1.26 1.22 -2.54 -4.76 4.37 3.81 8.47 -0.76 -2.26 4.61 3.79 8.35 -1.82
Mitotic Spindle -2.06 -2.04 -3.22 -1.03 0.52 -2.02 -2.64 -6.11 -5.04 -3.41 -4.43 -3.64 -8.13 -4.17 -2.88
None -2.93 -3.58 -3.37 0.91 -2.60 -0.88 -0.32 -1.25 -0.27 -1.19 -2.33 -0.31 -2.27 0.57 -2.41
Nuclear Periphery -0.61 -6.57 -2.85 -2.58 4.92 -1.88 -6.44 -2.96 -0.59 5.48 -1.58 -7.84 -3.66 -2.19 6.43
Nuclear Periphery Foci 0.44 -2.84 -0.08 -0.68 2.87 -2.82 -4.80 -4.08 -1.82 1.71 -3.56 -5.25 -6.18 -1.69 2.95
Nucleolus -0.47 -5.10 -1.47 -1.60 4.61 -0.33 -9.35 -1.23 -1.44 9.06 -0.61 -10.31 -1.80 -2.18 10.04
Nucleus 2.77 -6.48 -1.25 -11.55 6.93 7.58 -18.74 -5.00 -12.09 16.55 7.36 -20.40 -7.94 -16.52 16.94
Peroxisomes -3.30 -1.94 -0.64 1.51 1.45 -3.47 3.38 3.59 4.22 -0.34 -4.77 -0.11 0.16 4.14 0.21
Vacuole 7.13 4.63 3.18 -8.25 -2.90 -10.18 -10.29 -10.54 -0.41 1.35 -4.97 -8.36 -10.30 -5.64 0.25
Vacuole Periphery -6.73 -4.94 0.07 12.48 5.57 -21.11* -1.69 1.67 20.90 2.90 -21.33 -4.48 3.65 24.10 6.86
ORF
Human Ortholog
Description Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
Localization
Cell Percentages vacuole (13%), cytoplasm (10%), mixed (42%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00065)
Subcompartmental Group N/A

YDR338C

YDR338C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr338c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available