Standard name | |
---|---|
Human Ortholog | |
Description | Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; mutant phenotype is functionally complemented by rat PQLC2 vacuolar transporter |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 2 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 6 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 1 | 6 | 2 | 5 | 6 | 13 | 14 | 6 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 2 | 9 | 19 | 39 | 36 | 59 | 53 | 55 | 67 | 66 | 56 | 12 | 18 | 16 | 22 | 21 | 17 | 0 | 0 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 2 | 2 | 2 | 4 | 11 | 18 | 26 | 9 | 16 | 7 | 4 | 1 | 2 | 7 | 8 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2 | 1 | 6 | 2 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 13 | 19 | 12 | 3 | 8 | 2 | 6 | 3 | 5 | 6 | 24 | 25 |
Golgi | 0 | 3 | 4 | 18 | 23 | 15 | 15 | 5 | 1 | 8 | 4 | 3 | 6 | 7 | 13 | 15 | 19 | 6 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 18 | 1 | 4 | 8 | 11 | 29 | 55 | 20 | 62 | 44 | 55 | 45 | 4 | 3 | 1 | 4 | 4 | 7 | 1 | 2 | 3 | 3 | 3 | 6 |
Nucleus | 10 | 17 | 8 | 6 | 16 | 16 | 13 | 12 | 6 | 13 | 9 | 10 | 23 | 15 | 13 | 9 | 10 | 11 | 5 | 9 | 4 | 20 | 22 | 17 |
Nuclear Periphery | 26 | 12 | 15 | 37 | 18 | 29 | 43 | 33 | 24 | 21 | 21 | 13 | 23 | 62 | 55 | 28 | 44 | 29 | 12 | 5 | 10 | 5 | 7 | 16 |
Nucleolus | 18 | 5 | 11 | 7 | 4 | 1 | 3 | 3 | 4 | 1 | 2 | 0 | 17 | 24 | 14 | 5 | 5 | 5 | 2 | 2 | 2 | 8 | 6 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 5 |
Vac/Vac Membrane | 99 | 115 | 168 | 142 | 176 | 148 | 194 | 140 | 143 | 165 | 132 | 160 | 304 | 365 | 349 | 209 | 185 | 164 | 120 | 131 | 177 | 232 | 311 | 342 |
Unique Cell Count | 135 | 138 | 197 | 181 | 244 | 244 | 332 | 260 | 251 | 303 | 254 | 264 | 350 | 426 | 396 | 245 | 225 | 200 | 149 | 164 | 212 | 282 | 386 | 433 |
Labelled Cell Count | 176 | 158 | 228 | 241 | 293 | 293 | 404 | 303 | 316 | 353 | 314 | 301 | 403 | 516 | 484 | 306 | 302 | 251 | 149 | 164 | 212 | 282 | 386 | 433 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.9 | 15.8 | 12.7 | 12.5 | 13.2 | 11.3 | 10.4 | 9.9 | 9.0 | 9.3 | 9.3 | 9.1 | 14.4 | 13.4 | 13.7 | 16.8 | 16.4 | 15.2 | 13.8 | 13.4 | 14.7 |
Std Deviation (1e-4) | 2.6 | 4.2 | 3.4 | 2.8 | 3.6 | 3.5 | 3.0 | 2.8 | 2.6 | 2.7 | 2.3 | 2.5 | 4.9 | 3.9 | 3.9 | 6.5 | 7.7 | 5.9 | 3.7 | 4.4 | 4.4 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.05 | -0.17 | -0.3 | -0.36 | -0.5 | -0.45 | -0.46 | -0.48 | 0.18 | 0.08 | 0.11 | 0.4 | 0.37 | 0.25 | 0.12 | 0.08 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.2 | 3.8 | 3.5 | 4.4 | 4.9 | 5.2 | 5.3 | 6.1 | 5.1 | -0.7 | -0.2 | -0.3 | 1.8 | 1.9 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 2.2 | 2.6 | 4.0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 0.8 | 0 | 0 | 0.3 | 0 |
Golgi | 3.3 | 3.2 | 2.1 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 2.1 | 2.9 | 0 |
Mitochondria | 1.3 | 1.4 | 3.9 | 5.1 | 2.7 | 6.7 | 4.6 | 6.1 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0.9 |
Nucleus | -0.4 | 1.1 | 1.1 | -0.1 | 0.3 | -1.0 | 0.1 | -0.3 | -0.1 | 1.2 | -0.3 | -0.5 | -0.2 | 0.2 | 0.7 |
Nuclear Periphery | 3.6 | -0.1 | 1.5 | 1.9 | 1.8 | 0.7 | -0.3 | 0.3 | -1.2 | -0.5 | 2.4 | 2.2 | 1.3 | 3.5 | 2.2 |
Nucleolus | -0.8 | -2.3 | -3.3 | -3.2 | -2.7 | -2.3 | 0 | -3.0 | 0 | -0.4 | 0 | -1.2 | -2.0 | -1.8 | -1.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.7 | -3.3 | -5.7 | -6.4 | -7.1 | -6.5 | -7.1 | -7.4 | -5.8 | 0.5 | 0.1 | 1.0 | 0 | -0.8 | -0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3393 | 7.9763 | 7.05 | 6.1956 | 5.9298 | 6.6255 | 5.8297 | 8.2174 | 7.9231 | 6.5621 | 6.5677 | 7.5653 | 6.2979 | 10.1098 | 7.9176 | 7.0919 | 7.5706 | 7.9151 |
Actin | 0.0119 | 0.0001 | 0.006 | 0 | 0.0002 | 0.0023 | 0.0381 | 0.0078 | 0.0046 | 0.0001 | 0.0056 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0002 | 0.0002 | 0.0003 | 0.0017 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 |
Bud Neck | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0001 | 0.0007 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0004 | 0.0005 | 0.0006 | 0.0016 | 0.0008 | 0.0003 | 0.0005 | 0.0003 | 0.0013 | 0.0002 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.001 | 0.0001 | 0.0008 | 0.001 |
Bud Site | 0.0008 | 0.0004 | 0.0006 | 0.0007 | 0.0005 | 0.0001 | 0.0009 | 0.0018 | 0.0009 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0013 | 0.0011 | 0.0011 | 0.0007 | 0.0004 | 0.0007 | 0.0012 | 0.001 | 0.0011 | 0.0003 | 0.0003 | 0.0003 | 0.0012 | 0.0011 | 0.0007 | 0.0001 | 0.0005 | 0.0011 |
Cytoplasm | 0.0016 | 0.0022 | 0.0059 | 0.0011 | 0.0006 | 0.0002 | 0.0012 | 0.0024 | 0.0029 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0082 | 0.0003 | 0.0032 | 0.0002 | 0.001 | 0.0013 | 0.0056 | 0.0049 | 0.0077 | 0.0051 | 0.03 | 0.0005 | 0.0007 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0025 | 0.0003 | 0.0008 | 0.0002 | 0.0006 | 0.0004 | 0.0018 | 0.0007 | 0.0011 | 0.0009 | 0.0006 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0003 |
Endosome | 0.0267 | 0.0062 | 0.0201 | 0.015 | 0.0141 | 0.0109 | 0.0381 | 0.0095 | 0.026 | 0.0386 | 0.0256 | 0.0096 | 0.0148 | 0.0043 | 0.0127 | 0.0024 | 0.0037 | 0.005 |
Golgi | 0.0056 | 0.0002 | 0.0016 | 0.0001 | 0.0004 | 0.0011 | 0.0061 | 0.0006 | 0.0026 | 0.005 | 0.0126 | 0.0009 | 0.0009 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 |
Lipid Particles | 0.012 | 0.0011 | 0.0043 | 0.0001 | 0.0009 | 0.0051 | 0.0166 | 0.0011 | 0.0057 | 0.0247 | 0.0536 | 0.0072 | 0.002 | 0.0006 | 0.0054 | 0.0002 | 0.0002 | 0.0014 |
Mitochondria | 0.0125 | 0.0103 | 0.0234 | 0.0003 | 0.0014 | 0.0038 | 0.0479 | 0.0088 | 0.0136 | 0.0062 | 0.0035 | 0.0202 | 0.0116 | 0.0011 | 0.0067 | 0.0002 | 0.0004 | 0.0015 |
None | 0.001 | 0 | 0.0017 | 0 | 0.0002 | 0.0001 | 0.0013 | 0.0004 | 0.0014 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0139 | 0.0161 | 0.01 | 0.0024 | 0.0206 | 0.0318 | 0.0105 | 0.0113 | 0.0065 | 0.0087 | 0.0037 | 0.028 | 0.0041 | 0.0198 | 0.006 | 0.0066 | 0.0068 | 0.0181 |
Nucleolus | 0.0058 | 0.0013 | 0.0074 | 0.0007 | 0.007 | 0.001 | 0.0029 | 0.0014 | 0.0029 | 0.0009 | 0.0004 | 0.0042 | 0.004 | 0.0012 | 0.0024 | 0 | 0.0002 | 0.0014 |
Nucleus | 0.0056 | 0.0022 | 0.0132 | 0.0015 | 0.0394 | 0.0016 | 0.0026 | 0.0016 | 0.0024 | 0.0011 | 0.0007 | 0.0039 | 0.0034 | 0.0011 | 0.0011 | 0.0002 | 0.0007 | 0.0013 |
Peroxisomes | 0.0051 | 0.0007 | 0.001 | 0 | 0.0001 | 0.0093 | 0.0146 | 0.003 | 0.0099 | 0.0013 | 0.0433 | 0.0005 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.001 | 0 | 0.0043 | 0 | 0.0006 | 0.0024 | 0.005 | 0.0002 | 0.0046 | 0.0006 | 0.0104 | 0.0017 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3469 | 0.3384 | 0.3152 | 0.3056 | 0.2631 | 0.2119 | 0.2882 | 0.3049 | 0.2826 | 0.2348 | 0.2863 | 0.1527 | 0.2927 | 0.2552 | 0.2643 | 0.1645 | 0.2604 | 0.1981 |
Vacuole Periphery | 0.5364 | 0.6182 | 0.579 | 0.668 | 0.6472 | 0.7156 | 0.5154 | 0.6378 | 0.6211 | 0.6707 | 0.5217 | 0.7685 | 0.6627 | 0.7145 | 0.6975 | 0.8253 | 0.7256 | 0.7702 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.4935 | 23.7806 | 16.1117 | 22.5487 | 25.4931 | 33.5231 | 23.2604 | 19.7563 | 23.9401 | 31.3604 |
Translational Efficiency | 1.3769 | 1.278 | 1.378 | 1.0729 | 1.2478 | 0.832 | 1.2236 | 1.1359 | 0.8881 | 0.9887 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
608 | 1156 | 2925 | 922 | 2549 | 1477 | 271 | 1127 | 3157 | 2633 | 3196 | 2049 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1279.35 | 1251.64 | 1363.21 | 1319.51 | 1298.91 | 1257.31 | 1450.52 | 1383.01 | 1295.14 | 1254.82 | 1370.61 | 1354.44 |
Standard Deviation | 277.26 | 323.81 | 270.74 | 239.49 | 252.87 | 229.17 | 196.80 | 249.34 | 257.86 | 274.78 | 266.38 | 246.99 |
Intensity Change Log 2 | — | -0.031591 | 0.091597 | 0.044591 | — | -0.046961 | 0.159269 | 0.090510 | — | -0.039314 | 0.126086 | 0.067908 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000022 | 0.000074 | 0.000016 | 0.000083 | 0.000005 | 0.000054 | 0.000093 | 0.000147 | 0.000008 | 0.000063 | 0.000022 | 0.000118 |
Bud Neck | 0.000170 | 0.000743 | 0.000464 | 0.000776 | 0.000047 | 0.000928 | 0.007225 | 0.000754 | 0.000071 | 0.000846 | 0.001038 | 0.000764 |
Bud Site | 0.001317 | 0.003536 | 0.001869 | 0.003275 | 0.000335 | 0.001676 | 0.004426 | 0.002851 | 0.000524 | 0.002493 | 0.002086 | 0.003042 |
Cell Periphery | 0.000703 | 0.001253 | 0.000568 | 0.000598 | 0.000729 | 0.000346 | 0.000310 | 0.000401 | 0.000724 | 0.000744 | 0.000546 | 0.000489 |
Cytoplasm | 0.001911 | 0.004548 | 0.000518 | 0.000429 | 0.000374 | 0.001480 | 0.000916 | 0.001913 | 0.000670 | 0.002827 | 0.000552 | 0.001245 |
Cytoplasmic Foci | 0.000977 | 0.005993 | 0.000264 | 0.001516 | 0.000158 | 0.004731 | 0.001957 | 0.001464 | 0.000315 | 0.005285 | 0.000408 | 0.001487 |
Eisosomes | 0.000005 | 0.000014 | 0.000019 | 0.000013 | 0.000003 | 0.000011 | 0.000056 | 0.000008 | 0.000003 | 0.000012 | 0.000022 | 0.000010 |
Endoplasmic Reticulum | 0.000382 | 0.000733 | 0.000250 | 0.000399 | 0.000271 | 0.000326 | 0.000936 | 0.000383 | 0.000292 | 0.000505 | 0.000308 | 0.000390 |
Endosome | 0.003069 | 0.008672 | 0.001104 | 0.006889 | 0.000719 | 0.007566 | 0.010420 | 0.004172 | 0.001172 | 0.008051 | 0.001894 | 0.005394 |
Golgi | 0.001488 | 0.006395 | 0.001288 | 0.020223 | 0.000723 | 0.005354 | 0.002681 | 0.007823 | 0.000870 | 0.005811 | 0.001406 | 0.013403 |
Lipid Particles | 0.000152 | 0.000805 | 0.000578 | 0.000664 | 0.000251 | 0.000714 | 0.000357 | 0.000380 | 0.000232 | 0.000754 | 0.000559 | 0.000508 |
Mitochondria | 0.005300 | 0.006574 | 0.002574 | 0.011050 | 0.001555 | 0.008031 | 0.007105 | 0.003687 | 0.002277 | 0.007392 | 0.002958 | 0.007000 |
Mitotic Spindle | 0.000045 | 0.000347 | 0.000107 | 0.000552 | 0.000014 | 0.001032 | 0.000915 | 0.000402 | 0.000020 | 0.000731 | 0.000176 | 0.000469 |
None | 0.000140 | 0.001150 | 0.000304 | 0.000332 | 0.000136 | 0.000381 | 0.000278 | 0.000170 | 0.000137 | 0.000719 | 0.000302 | 0.000243 |
Nuclear Periphery | 0.000243 | 0.000655 | 0.000812 | 0.002082 | 0.000294 | 0.001676 | 0.003372 | 0.001075 | 0.000284 | 0.001228 | 0.001029 | 0.001528 |
Nuclear Periphery Foci | 0.000118 | 0.001208 | 0.000379 | 0.001415 | 0.000095 | 0.002524 | 0.001849 | 0.000585 | 0.000100 | 0.001946 | 0.000504 | 0.000959 |
Nucleolus | 0.000374 | 0.002764 | 0.002176 | 0.001388 | 0.000364 | 0.003466 | 0.030831 | 0.001682 | 0.000366 | 0.003158 | 0.004605 | 0.001550 |
Nucleus | 0.000334 | 0.003079 | 0.001545 | 0.000622 | 0.000219 | 0.001415 | 0.015457 | 0.000927 | 0.000241 | 0.002146 | 0.002724 | 0.000790 |
Peroxisomes | 0.000190 | 0.000667 | 0.000058 | 0.000176 | 0.000015 | 0.000414 | 0.002378 | 0.000293 | 0.000049 | 0.000525 | 0.000254 | 0.000241 |
Vacuole | 0.183585 | 0.223415* | 0.237665* | 0.360615* | 0.156726 | 0.194079 | 0.436836* | 0.361573* | 0.161899 | 0.206959* | 0.254553* | 0.361142* |
Vacuole Periphery | 0.799475* | 0.727374* | 0.747441* | 0.586903* | 0.836966* | 0.763796* | 0.471604* | 0.609309* | 0.829746* | 0.747805* | 0.724052* | 0.599227* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.97 | 0.85 | -3.35 | -0.14 | -3.91 | -4.72 | -6.46 | -1.64 | -1.06 | -0.62 | -5.71 | -3.99 | -2.27 | -1.08 | -1.98 |
Bud Neck | -4.16 | -3.84 | -5.75 | -0.18 | -2.58 | -5.85 | -6.17 | -7.37 | 0.95 | 5.55 | -7.53 | -7.93 | -9.96 | 0.67 | 2.01 |
Bud Site | -3.04 | -0.97 | -2.27 | 0.21 | -1.76 | -4.36 | -3.96 | -2.98 | -1.45 | 1.30 | -6.09 | -4.59 | -4.29 | -1.03 | -1.53 |
Cell Periphery | -1.73 | 0.64 | 0.45 | 2.49 | -0.27 | 4.57 | 3.25 | 2.64 | -0.50 | -0.62 | -0.16 | 2.16 | 2.30 | 1.95 | 0.69 |
Cytoplasm | -1.46 | 1.18 | 1.25 | 3.35 | 0.55 | -2.29 | -0.97 | -2.02 | -0.99 | -1.31 | -3.29 | 0.41 | -1.47 | 1.26 | -1.75 |
Cytoplasmic Foci | -4.16 | 2.05 | -1.12 | 3.76 | -3.95 | -5.61 | -3.19 | -2.88 | 3.52 | 0.77 | -7.23 | -1.03 | -3.95 | 5.15 | -3.67 |
Eisosomes | -3.93 | -7.94 | -3.93 | 0.30 | 2.87 | -4.01 | -6.53 | -5.28 | 1.29 | 5.86 | -6.27 | -13.84 | -7.27 | 1.11 | 7.38 |
Endoplasmic Reticulum | -2.40 | 1.45 | -0.17 | 2.63 | -2.90 | -1.49 | -1.81 | -1.32 | -0.62 | 1.48 | -3.61 | -0.48 | -1.78 | 1.54 | -1.28 |
Endosome | -4.27 | 2.59 | -3.24 | 1.28 | -6.24 | -7.48 | -4.39 | -5.69 | 3.16 | 2.83 | -9.56 | -2.57 | -7.60 | 3.08 | -5.97 |
Golgi | -5.44 | 0.45 | -6.82 | -4.86 | -6.95 | -7.37 | -2.95 | -6.05 | -1.87 | -3.86 | -9.52 | -2.28 | -8.98 | -5.14 | -8.60 |
Lipid Particles | -5.85 | -12.29 | -4.72 | 1.13 | -0.80 | -5.09 | -2.51 | -1.20 | 2.38 | -0.20 | -7.44 | -13.41 | -3.59 | 2.52 | 0.63 |
Mitochondria | -0.48 | 1.21 | -2.02 | -1.95 | -4.56 | -5.50 | -3.47 | -2.72 | 3.35 | 2.02 | -4.96 | -1.01 | -4.53 | 0.31 | -4.08 |
Mitotic Spindle | -2.47 | -1.98 | -2.20 | -0.66 | -1.91 | -2.79 | -2.18 | -3.60 | 1.50 | 1.03 | -3.39 | -3.50 | -3.88 | 1.00 | -2.48 |
None | -3.02 | -4.82 | -3.77 | 2.44 | -0.62 | -5.43 | -3.87 | -1.08 | 5.10 | 3.37 | -3.90 | -6.19 | -3.46 | 3.18 | 2.28 |
Nuclear Periphery | -2.72 | -8.28 | -3.56 | -2.66 | -2.45 | -3.69 | -4.06 | -3.93 | 1.39 | 2.91 | -4.28 | -8.51 | -4.84 | -0.92 | -1.88 |
Nuclear Periphery Foci | -3.48 | -3.41 | -4.00 | -0.26 | -3.12 | -3.98 | -5.99 | -6.04 | 3.14 | 4.12 | -5.05 | -5.49 | -5.64 | 2.54 | -2.72 |
Nucleolus | -4.47 | -7.49 | -3.25 | 2.30 | 2.14 | -4.07 | -5.54 | -3.25 | 2.02 | 5.28 | -5.74 | -8.05 | -4.53 | 2.89 | 5.14 |
Nucleus | -3.28 | -6.38 | -1.86 | 2.94 | 4.69 | -3.69 | -4.54 | -4.17 | 1.14 | 4.30 | -4.65 | -7.54 | -4.91 | 3.13 | 5.48 |
Peroxisomes | -2.01 | 1.31 | 0.12 | 2.16 | -2.21 | -4.21 | -2.75 | -1.42 | 0.57 | 2.37 | -4.00 | -2.69 | -1.71 | 1.94 | 0.12 |
Vacuole | -4.10 | -6.31 | -16.46 | -14.49 | -14.85 | -6.50 | -20.20 | -27.32 | -20.19 | 4.98 | -9.41 | -19.53 | -34.09 | -24.70 | -17.22 |
Vacuole Periphery | 6.37 | 5.51 | 17.90 | 12.60 | 17.69* | 10.47 | 22.64 | 28.34* | 16.22 | -7.90 | 14.30 | 20.40 | 36.45 | 20.48 | 18.42* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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