Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.82 0.77 0.78 0.8 0.8 0.8 – 0.79 0.78 0.83 0.79 0.72 0.8 0.69 0.73 0.89 0.92 0.89 0.44 0.42 0.44 0.4 0.37 0.38
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0.06 0.16 0 0 0.09 0 – 0 0 0 0 0 0.18 0.2 0.2 0.07 0.06 0 0.27 0.34 0.22 0.26 0.29 0.28
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.17 0.3 0.13 0.31 – 0.43 0.41 0.36 0.41 0.49 0 0 0 0 0 0 0.05 0.06 0.12 0.1 0.06 0.07
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0.05 0.09 0 0.05 0 – 0 0 0 0 0 0 0.09 0.05 0 0 0 0.07 0 0.07 0.06 0.09 0.05
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0.1 0.09 0.06 0.12 0.13 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 14 7 3 1 0 3
Bud 9 7 17 13 8 17 – 21 14 27 17 17 0 1 0 0 1 0 6 1 1 1 3 4
Bud Neck 6 0 11 0 2 2 – 2 0 2 1 1 0 1 1 1 1 0 7 3 2 1 0 1
Bud Site 0 0 0 0 0 1 – 1 3 2 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 – 0 1 1 4 2 0 0 0 3 3 2 1 0 0 0 0 0
Cytoplasm 581 323 328 326 404 437 – 677 517 638 476 427 144 107 166 141 208 144 384 194 212 80 98 113
Endoplasmic Reticulum 4 5 2 0 1 1 – 0 0 0 1 0 2 2 0 8 11 11 1 0 3 1 1 2
Endosome 45 65 2 12 44 10 – 8 9 6 5 2 32 31 45 11 13 6 237 159 107 52 77 84
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 2 2 2 0 1 3 0 0 3 6
Mitochondria 27 6 70 122 65 172 – 367 268 275 246 291 0 1 1 6 4 7 39 28 59 19 16 20
Nucleus 0 0 2 0 3 3 – 7 1 2 3 2 0 1 0 0 0 2 1 0 1 0 0 0
Nuclear Periphery 0 2 0 0 0 0 – 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 1 0 0 1 0 – 2 0 0 1 1 0 0 0 0 1 1 0 0 0 0 1 0
Peroxisomes 0 1 0 4 6 8 – 1 2 4 1 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 46 22 36 12 26 16 – 13 14 18 7 9 8 14 12 4 5 4 57 17 34 11 22 15
Vac/Vac Membrane 25 17 3 5 8 10 – 5 4 6 3 7 8 8 10 4 6 3 87 39 29 23 34 41
Unique Cell Count 709 419 420 409 505 548 859 661 773 606 592 179 156 226 158 227 161 865 465 480 202 269 299
Labelled Cell Count 745 451 472 494 569 677 1105 833 981 766 761 194 168 238 180 255 180 865 465 480 202 269 299


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.6 3.3 3.2 4.0 3.2 – 3.3 2.9 3.2 3.4 2.9 5.1 5.3 5.0 5.6 5.5 5.3 4.6 5.1 5.3
Std Deviation (1e-4) 0.4 0.8 0.7 1.2 1.4 0.9 – 1.2 1.1 0.9 6.4 0.6 1.1 1.4 0.9 1.4 1.2 1.4 1.1 1.3 1.4
Intensity Change (Log2) – – – -0.05 0.26 -0.06 – 0.0 -0.21 -0.07 0.04 -0.19 0.62 0.66 0.59 0.74 0.73 0.67 0.48 0.61 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1903 0.64 0.4311 0.152 0.2746 0.4449 -0.0991 -0.0136 0.0383 0.184 0.6803 -0.1221 0.918 1.3581 1.2632 1.5829 0.946 1.2171
Actin 0.009 0.0006 0.0004 0.0021 0.0001 0.0016 0.0161 0.0044 0.0119 0.0079 0.0007 0.0054 0.012 0.0006 0.0018 0.0001 0.0001 0.0013
Bud 0.0003 0.0051 0.0007 0.0004 0.0001 0.0021 0.0041 0.0032 0.0015 0.0005 0.0003 0.0004 0.0018 0.0009 0.0021 0.0002 0.0008 0.0002
Bud Neck 0.0006 0.0008 0.0005 0.0091 0.0002 0.002 0.0051 0.0022 0.0036 0.0004 0.0012 0.0096 0.0013 0.0004 0.0002 0.0002 0.0004 0.0033
Bud Periphery 0.0003 0.0018 0.0009 0.0003 0.0001 0.0021 0.0097 0.0055 0.0017 0.0007 0.0003 0.0009 0.0026 0.001 0.0022 0.0002 0.0009 0.0002
Bud Site 0.0007 0.0056 0.001 0.0017 0.0001 0.002 0.0174 0.0284 0.0137 0.0007 0.0016 0.0002 0.002 0.0026 0.0028 0.0001 0.0009 0.0002
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0006 0.0004 0.0005 0.0001 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.1035 0.1377 0.1354 0.0876 0.0675 0.132 0.0735 0.0956 0.0793 0.068 0.1449 0.1045 0.1294 0.1592 0.1571 0.1184 0.1541 0.1647
Cytoplasmic Foci 0.2337 0.2294 0.2706 0.3286 0.3539 0.3166 0.2231 0.2079 0.2767 0.2724 0.2344 0.2489 0.2202 0.2588 0.2202 0.194 0.2138 0.2782
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0006 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0036 0.0021 0.0024 0.0006 0.0005 0.002 0.0039 0.0017 0.0022 0.0023 0.0018 0.0016 0.003 0.0023 0.0039 0.0015 0.0027 0.0019
Endosome 0.2128 0.179 0.2031 0.2606 0.2939 0.192 0.1319 0.1347 0.1381 0.2379 0.2812 0.1281 0.2201 0.1747 0.1837 0.1994 0.296 0.1507
Golgi 0.0067 0.0056 0.0075 0.0106 0.0083 0.005 0.0105 0.0046 0.0093 0.0207 0.0151 0.0067 0.0053 0.0061 0.0068 0.0056 0.0064 0.0049
Lipid Particles 0.0214 0.0273 0.018 0.0046 0.0156 0.0173 0.0253 0.0179 0.0205 0.0249 0.0338 0.0205 0.0253 0.0112 0.012 0.008 0.0029 0.03
Mitochondria 0.0027 0.0013 0.0041 0.0011 0.0006 0.0013 0.0126 0.0023 0.005 0.0072 0.0033 0.0052 0.0032 0.0031 0.0027 0.0035 0.0029 0.0015
None 0.3693 0.3635 0.3165 0.2535 0.2335 0.2862 0.3994 0.4444 0.369 0.3072 0.238 0.4036 0.3371 0.3461 0.369 0.4435 0.2589 0.3281
Nuclear Periphery 0.0061 0.0017 0.0016 0.0008 0.0007 0.0019 0.0106 0.0018 0.0031 0.0071 0.0011 0.0024 0.0013 0.0016 0.0024 0.0011 0.0019 0.0014
Nucleolus 0.0006 0.0018 0.0008 0.0007 0.0002 0.0011 0.0032 0.0018 0.0014 0.0006 0.0014 0.0016 0.0013 0.0007 0.0006 0.0002 0.0004 0.0008
Nucleus 0.0036 0.0025 0.0017 0.0069 0.0006 0.0051 0.0056 0.0036 0.004 0.0037 0.0014 0.002 0.0022 0.0017 0.0014 0.0008 0.0026 0.0031
Peroxisomes 0.0085 0.0102 0.017 0.0079 0.0114 0.0163 0.0247 0.0116 0.039 0.0126 0.0105 0.0338 0.0046 0.0102 0.016 0.0081 0.0031 0.011
Punctate Nuclear 0.0037 0.0073 0.0038 0.0016 0.0035 0.0055 0.0076 0.0162 0.0096 0.0037 0.0025 0.0185 0.0022 0.0039 0.003 0.0009 0.0012 0.0097
Vacuole 0.0111 0.0152 0.0122 0.0196 0.0072 0.0065 0.0119 0.0105 0.0081 0.0167 0.0241 0.005 0.0229 0.0132 0.0099 0.0115 0.0423 0.0076
Vacuole Periphery 0.0014 0.0014 0.0017 0.0016 0.0022 0.0013 0.0026 0.0011 0.0015 0.0045 0.0024 0.001 0.0016 0.0014 0.002 0.0024 0.0076 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.4367 16.0386 15.8685 32.2378 13.5695 20.9202 20.2708 26.4508 37.1363 28.1389
Translational Efficiency 1.2276 1.2832 1.2756 0.8244 1.3444 1.1936 0.6123 0.9042 0.7584 0.8684

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2022 1708 80 142 2136 2505 2448 1218 4158 4213 2528 1360

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 606.51 724.42 864.42 875.50 681.99 726.75 780.07 794.42 645.28 725.81 782.74 802.89
Standard Deviation 74.57 87.65 88.10 106.78 76.68 91.03 90.89 109.91 84.54 89.68 91.99 112.36
Intensity Change Log 2 — 0.256295 0.511201 0.529576 — 0.091709 0.193853 0.220151 — 0.171524 0.351948 0.374086

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000190 0.000377 0.001016 0.000411 0.000711 0.000105 0.000188 0.001298 0.000458 0.000216 0.000214 0.001206
Bud Neck 0.040005 0.030052 0.003141 0.003461 0.034286 0.035942 0.028118 0.008392 0.037067 0.033554 0.027327 0.007877
Bud Site 0.003765 0.010424 0.005693 0.007673 0.003736 0.005712 0.020080 0.030971 0.003750 0.007622 0.019625 0.028539
Cell Periphery 0.000175 0.000075 0.000343 0.000188 0.000093 0.000115 0.000066 0.000141 0.000133 0.000099 0.000075 0.000146
Cytoplasm 0.319986* 0.164621* 0.200037* 0.310312* 0.203739* 0.210298* 0.498980* 0.500924* 0.260269* 0.191780* 0.489520* 0.481022*
Cytoplasmic Foci 0.325773* 0.343179* 0.088364 0.050641 0.347306* 0.319674* 0.042316 0.043450 0.336835* 0.329203* 0.043774 0.044201
Eisosomes 0.000486 0.000118 0.000212 0.000102 0.000219 0.000096 0.000030 0.000056 0.000349 0.000105 0.000035 0.000060
Endoplasmic Reticulum 0.001813 0.001109 0.008910 0.008623 0.001504 0.001233 0.001288 0.001385 0.001654 0.001183 0.001529 0.002140
Endosome 0.058674 0.177996 0.030802 0.044014 0.152175 0.155276 0.018663 0.011294 0.106706 0.164487 0.019047 0.014710
Golgi 0.010542 0.016022 0.000516 0.000176 0.009671 0.012333 0.000567 0.002262 0.010095 0.013828 0.000566 0.002044
Lipid Particles 0.045185 0.023382 0.014465 0.004274 0.041123 0.026636 0.002420 0.001892 0.043098 0.025317 0.002802 0.002140
Mitochondria 0.010471 0.021906 0.000498 0.000174 0.014524 0.015050 0.002738 0.004510 0.012553 0.017830 0.002667 0.004057
Mitotic Spindle 0.001386 0.019573 0.003784 0.016188 0.002975 0.004436 0.023380 0.031403 0.002203 0.010573 0.022760 0.029814
None 0.001708 0.002147 0.002590 0.004912 0.000769 0.002183 0.003552 0.003632 0.001226 0.002168 0.003521 0.003766
Nuclear Periphery 0.000514 0.000907 0.002907 0.001551 0.000695 0.000965 0.002858 0.001523 0.000607 0.000941 0.002860 0.001526
Nuclear Periphery Foci 0.000308 0.000866 0.008914 0.008390 0.000519 0.000643 0.001411 0.001440 0.000416 0.000734 0.001649 0.002166
Nucleolus 0.001171 0.002224 0.002910 0.000509 0.001747 0.003888 0.004094 0.000850 0.001467 0.003214 0.004056 0.000814
Nucleus 0.088472 0.037361 0.457896* 0.198452* 0.061679 0.052836 0.249332* 0.213867* 0.074708 0.046562 0.255932* 0.212258*
Peroxisomes 0.013518 0.036832 0.015536 0.002775 0.025698 0.017929 0.004286 0.001975 0.019775 0.025593 0.004642 0.002059
Vacuole 0.073769 0.106888 0.150810 0.336958* 0.094640 0.129427 0.094835 0.137354 0.084490 0.120290 0.096606 0.158195
Vacuole Periphery 0.002089 0.003940 0.000655 0.000216 0.002192 0.005221 0.000798 0.001383 0.002142 0.004702 0.000794 0.001261

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.33 -4.50 -3.59 -1.80 2.58 1.49 1.25 -0.04 -2.96 -2.48 1.15 1.06 -0.91 -2.62 -2.51
Bud Neck 3.44 14.50 9.84 7.69 -1.52 -0.82 -0.44 13.60 16.17 14.28 1.76 1.18 18.24 19.00 14.21
Bud Site -5.67 -1.66 -2.51 0.69 -0.70 -3.21 -6.66 -5.95 -5.06 -1.24 -6.46 -6.85 -6.21 -4.23 -1.07
Cell Periphery 4.42 -3.56 1.21 -4.24 4.60 -0.54 2.31 -0.75 0.25 -7.09 1.43 4.82 1.76 -0.49 -6.48
Cytoplasm 20.11 6.01 4.98 -2.33 -1.09 -1.09 -29.87 -21.14 -20.52 2.52 12.77 -23.93 -14.60 -22.52 3.87
Cytoplasmic Foci -2.34 15.80 30.73 31.49 2.86 4.20 56.31 57.83 57.03 4.15 1.64 72.12 74.09 72.22 4.36
Eisosomes 14.83 7.86 14.62 0.73 4.99 10.87 16.39 13.83 9.15 -10.35 18.02 22.73 20.73 11.03 -9.44
Endoplasmic Reticulum 3.27 -5.84 -5.68 -6.31 1.43 1.31 -1.24 -3.54 -6.64 -3.28 3.17 -2.37 -6.02 -9.38 -4.17
Endosome -19.98 2.63 6.48 20.17 1.87 -0.36 26.55 29.55 35.37 8.07 -13.06 27.81 32.58 45.66 7.40
Golgi -3.89 12.13 12.70 15.51 2.87 -2.24 11.19 9.93 13.55 -1.59 -4.10 16.35 14.33 18.90 -1.48
Lipid Particles 8.53 9.29 19.59 13.03 4.72 6.55 21.16 21.79 18.10 5.03 10.54 28.68 29.78 22.96 5.70
Mitochondria -6.15 10.63 10.90 14.03 3.54 -0.25 8.28 7.62 11.28 0.25 -4.25 11.41 9.75 15.17 0.51
Mitotic Spindle -7.58 -1.57 -2.00 0.19 -1.02 -1.60 -10.20 -6.12 -5.61 1.47 -7.42 -10.98 -6.77 -3.47 1.47
None -0.66 -0.52 -1.54 -1.05 -1.26 -3.38 -10.40 -7.29 -2.62 0.93 -2.45 -10.13 -6.57 -2.67 0.62
Nuclear Periphery -2.48 -4.86 -8.25 -5.71 0.81 -2.53 -19.82 -13.06 -10.05 7.13 -3.72 -21.19 -15.42 -11.00 7.32
Nuclear Periphery Foci -4.84 -4.59 -5.84 -5.35 1.07 -1.23 -5.08 -5.16 -4.71 1.31 -4.25 -6.44 -8.05 -6.50 0.33
Nucleolus -4.89 -2.91 -0.80 0.65 0.84 -4.56 -9.59 2.70 6.57 11.02 -6.18 -10.52 1.82 6.94 10.94
Nucleus 15.41 -9.00 -5.48 -8.20 5.42 2.92 -36.23 -25.93 -27.54 2.00 12.50 -36.10 -25.15 -29.73 3.34
Peroxisomes -11.93 -0.53 9.28 18.19 1.91 4.84 14.82 17.55 20.30 6.45 -4.85 15.46 21.24 27.50 6.42
Vacuole -8.31 -6.23 -14.90 -13.51 -8.07 -8.05 -8.51 -18.62 -13.97 -13.10 -12.05 -12.74 -23.72 -18.59 -15.70
Vacuole Periphery -6.20 7.84 10.84 16.49 6.18 -8.38 9.96 6.55 11.86 -0.28 -10.29 11.19 7.56 14.63 -0.05
Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Cnl1

Cnl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cnl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available