Standard name | |
---|---|
Human Ortholog | |
Description | NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 1 | 0 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 6 | 11 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 3 | 0 | 6 | 8 | 11 | 15 | 14 | 9 | 15 | 14 | 5 | 4 | 9 | 6 | 14 | 19 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 259 | 182 | 138 | 306 | 450 | 346 | 472 | 418 | 309 | 307 | 253 | 341 | 448 | 589 | 693 | 368 | 390 | 354 | 247 | 177 | 145 | 431 | 585 | 627 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 6 | 8 | 12 | 28 | 13 | 13 | 4 | 12 | 0 | 2 | 3 | 7 | 8 | 11 | 1 | 0 | 0 | 1 | 2 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 3 |
Golgi | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 2 | 3 |
Mitochondria | 2 | 1 | 0 | 1 | 1 | 7 | 18 | 18 | 27 | 29 | 43 | 24 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleus | 0 | 1 | 2 | 2 | 3 | 3 | 10 | 14 | 8 | 19 | 7 | 13 | 1 | 3 | 3 | 0 | 1 | 1 | 0 | 1 | 2 | 2 | 3 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 11 | 21 | 31 | 72 | 113 | 73 | 87 | 51 | 129 | 2 | 6 | 2 | 7 | 7 | 4 | 0 | 0 | 1 | 1 | 2 | 3 |
Unique Cell Count | 261 | 183 | 138 | 313 | 470 | 388 | 570 | 583 | 424 | 442 | 352 | 515 | 450 | 600 | 698 | 375 | 401 | 361 | 253 | 183 | 151 | 442 | 607 | 658 |
Labelled Cell Count | 266 | 185 | 144 | 321 | 487 | 405 | 596 | 617 | 450 | 471 | 379 | 554 | 456 | 609 | 710 | 391 | 424 | 390 | 253 | 183 | 151 | 442 | 607 | 658 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 13.3 | 13.1 | 11.9 | 11.9 | 10.8 | 10.5 | 10.8 | 9.9 | 9.8 | 9.7 | 10.5 | 16.7 | 18.0 | 19.4 | 32.1 | 35.4 | 35.0 | 13.6 | 14.6 | 15.3 |
Std Deviation (1e-4) | 1.8 | 2.3 | 1.9 | 2.1 | 2.0 | 1.8 | 1.9 | 2.2 | 2.1 | 2.2 | 2.4 | 2.6 | 3.1 | 3.7 | 3.9 | 8.8 | 10.9 | 10.7 | 2.1 | 2.6 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.13 | -0.28 | -0.32 | -0.28 | -0.4 | -0.42 | -0.42 | -0.32 | 0.36 | 0.46 | 0.57 | 1.3 | 1.44 | 1.42 | 0.06 | 0.16 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 |
Cytoplasm | -1.8 | -2.5 | -4.0 | -5.2 | -7.1 | -6.9 | -7.4 | -7.0 | -8.0 | -0.8 | -1.6 | -1.0 | -1.6 | -2.0 | -1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.1 | 4.3 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.4 | 4.4 | 5.6 | 5.2 | 5.7 | 4.7 | 6.6 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.7616 | 11.4986 | 11.057 | 8.879 | 9.5717 | 10.1821 | 8.9188 | 11.5424 | 10.6318 | 9.8568 | 7.9218 | 10.5161 | 10.5971 | 10.7436 | 11.7391 | 10.623 | 10.285 | 12.33 |
Actin | 0.0002 | 0.0004 | 0.0017 | 0.0005 | 0.0006 | 0.0003 | 0.0126 | 0.0018 | 0.0076 | 0.0192 | 0.0009 | 0.0007 | 0.0103 | 0.0009 | 0.0014 | 0.0029 | 0.0585 | 0.0004 |
Bud | 0.0001 | 0.0002 | 0.0038 | 0.0075 | 0.0002 | 0.0008 | 0.0012 | 0.0003 | 0.0019 | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.0086 | 0.0018 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0002 | 0.0002 | 0.0003 | 0.0014 | 0.001 | 0.0021 | 0.0025 | 0.0002 | 0.001 | 0.0009 | 0.0006 | 0.0013 | 0.001 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0006 |
Bud Periphery | 0 | 0 | 0.0006 | 0.003 | 0.0001 | 0.0002 | 0.0007 | 0.0001 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0012 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0001 | 0.0006 | 0.0009 | 0.0007 | 0.0002 | 0.0003 | 0.0024 | 0.0011 | 0.0027 | 0.0003 | 0.0005 | 0.0001 | 0.001 | 0.0021 | 0.0022 | 0.0002 | 0.0002 | 0 |
Cell Periphery | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.9326 | 0.9698 | 0.948 | 0.8931 | 0.9184 | 0.9202 | 0.8122 | 0.9755 | 0.9245 | 0.8752 | 0.7045 | 0.948 | 0.8982 | 0.9554 | 0.9605 | 0.9064 | 0.8944 | 0.9593 |
Cytoplasmic Foci | 0.0037 | 0.002 | 0.0022 | 0.0078 | 0.0023 | 0.0077 | 0.0419 | 0.0033 | 0.0111 | 0.0144 | 0.0381 | 0.0052 | 0.0079 | 0.0028 | 0.0033 | 0.0044 | 0.0014 | 0.0014 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0054 | 0.0018 | 0.001 | 0.004 | 0.0024 | 0.0022 | 0.0049 | 0.0007 | 0.0025 | 0.004 | 0.0063 | 0.0013 | 0.0033 | 0.0011 | 0.0009 | 0.0035 | 0.0011 | 0.0015 |
Endosome | 0.0052 | 0.0009 | 0.0011 | 0.0179 | 0.0012 | 0.003 | 0.0366 | 0.0006 | 0.0106 | 0.0232 | 0.169 | 0.004 | 0.0089 | 0.0009 | 0.0051 | 0.0123 | 0.0009 | 0.0041 |
Golgi | 0.0001 | 0.0001 | 0.0005 | 0.0008 | 0 | 0.0002 | 0.0076 | 0.0003 | 0.0023 | 0.004 | 0.0134 | 0.0019 | 0.0015 | 0.0001 | 0.001 | 0.0019 | 0.0018 | 0.0003 |
Lipid Particles | 0.0002 | 0 | 0.0011 | 0.0001 | 0 | 0.0004 | 0.0134 | 0.0001 | 0.0029 | 0.0034 | 0.0211 | 0.0009 | 0.0015 | 0 | 0.0005 | 0.0022 | 0.0012 | 0.0001 |
Mitochondria | 0.0002 | 0.0001 | 0.0053 | 0.0005 | 0.0004 | 0.0002 | 0.0034 | 0.0069 | 0.0015 | 0.0066 | 0.0046 | 0.0005 | 0.0055 | 0.0003 | 0.0004 | 0.0028 | 0.0006 | 0.0004 |
None | 0.0058 | 0.0014 | 0.0016 | 0.0034 | 0.0032 | 0.0017 | 0.0102 | 0.0012 | 0.0039 | 0.0021 | 0.0025 | 0.0015 | 0.0031 | 0.0017 | 0.0012 | 0.0021 | 0.0006 | 0.0007 |
Nuclear Periphery | 0.0057 | 0.0027 | 0.0025 | 0.0062 | 0.006 | 0.005 | 0.006 | 0.0008 | 0.0029 | 0.0041 | 0.0068 | 0.0032 | 0.0077 | 0.0037 | 0.0024 | 0.0231 | 0.0042 | 0.008 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0048 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 |
Nucleus | 0.0373 | 0.0181 | 0.0259 | 0.0452 | 0.0615 | 0.0511 | 0.0155 | 0.0055 | 0.0151 | 0.0212 | 0.0155 | 0.0262 | 0.0331 | 0.018 | 0.0161 | 0.027 | 0.0281 | 0.0205 |
Peroxisomes | 0.0001 | 0.0001 | 0.0019 | 0.0002 | 0 | 0.0002 | 0.0147 | 0.0007 | 0.0037 | 0.011 | 0.0016 | 0.0002 | 0.001 | 0.0002 | 0.0006 | 0.0002 | 0.0028 | 0 |
Punctate Nuclear | 0.001 | 0.0006 | 0.0006 | 0.002 | 0.0014 | 0.003 | 0.0091 | 0.0005 | 0.002 | 0.0013 | 0.0026 | 0.0038 | 0.006 | 0.0011 | 0.0009 | 0.0022 | 0.0028 | 0.0006 |
Vacuole | 0.0018 | 0.0008 | 0.0007 | 0.0047 | 0.0007 | 0.001 | 0.0033 | 0.0002 | 0.0016 | 0.006 | 0.0081 | 0.0006 | 0.0027 | 0.0011 | 0.0006 | 0.0057 | 0.0003 | 0.0011 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0011 | 0.0001 | 0.0006 | 0.0019 | 0.0029 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0021 | 0.0001 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.1044 | 35.7154 | 60.0201 | 65.2429 | 51.8007 | 76.3382 | 57.6196 | 61.2911 | 78.2062 | 67.4088 |
Translational Efficiency | 2.0185 | 3.1839 | 1.7344 | 2.063 | 2.554 | 1.9952 | 2.2724 | 2.1042 | 1.876 | 1.9059 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1432 | 1074 | 2161 | 100 | 1589 | 2088 | 413 | 1149 | 3021 | 3162 | 2574 | 1249 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1116.03 | 1292.62 | 1332.04 | 1995.19 | 1073.00 | 1230.57 | 1643.61 | 1349.05 | 1093.40 | 1251.65 | 1382.03 | 1400.78 |
Standard Deviation | 201.55 | 227.48 | 214.14 | 329.72 | 168.56 | 200.15 | 316.47 | 394.90 | 186.18 | 211.88 | 260.09 | 427.69 |
Intensity Change Log 2 | — | 0.211922 | 0.255262 | 0.838150 | — | 0.197677 | 0.615218 | 0.330294 | — | 0.204957 | 0.442913 | 0.611387 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000255 | 0.000260 | 0.000153 | 0.000835 | 0.000090 | 0.000206 | 0.000426 | 0.000166 | 0.000168 | 0.000224 | 0.000197 | 0.000220 |
Bud Neck | 0.000451 | 0.000492 | 0.000993 | 0.003625 | 0.000612 | 0.000713 | 0.001933 | 0.000993 | 0.000536 | 0.000638 | 0.001144 | 0.001204 |
Bud Site | 0.002920 | 0.004230 | 0.002780 | 0.004842 | 0.002716 | 0.004374 | 0.004254 | 0.003585 | 0.002813 | 0.004325 | 0.003016 | 0.003686 |
Cell Periphery | 0.000061 | 0.000061 | 0.000049 | 0.000726 | 0.000037 | 0.000036 | 0.000288 | 0.000125 | 0.000049 | 0.000045 | 0.000087 | 0.000173 |
Cytoplasm | 0.864538* | 0.857585* | 0.778517* | 0.418387* | 0.858117* | 0.872376* | 0.525793* | 0.810504* | 0.861160* | 0.867352* | 0.737967* | 0.779110* |
Cytoplasmic Foci | 0.025110 | 0.024642 | 0.006299 | 0.018023 | 0.030780 | 0.023887 | 0.022466 | 0.013029 | 0.028092 | 0.024143 | 0.008893 | 0.013429 |
Eisosomes | 0.000005 | 0.000008 | 0.000005 | 0.000010 | 0.000004 | 0.000004 | 0.000067 | 0.000005 | 0.000005 | 0.000005 | 0.000015 | 0.000006 |
Endoplasmic Reticulum | 0.000812 | 0.000745 | 0.000257 | 0.000454 | 0.000470 | 0.000172 | 0.006290 | 0.000389 | 0.000632 | 0.000367 | 0.001225 | 0.000394 |
Endosome | 0.000994 | 0.001867 | 0.000688 | 0.001629 | 0.000669 | 0.000956 | 0.002798 | 0.001902 | 0.000823 | 0.001265 | 0.001026 | 0.001880 |
Golgi | 0.000486 | 0.001436 | 0.000868 | 0.000668 | 0.001108 | 0.001152 | 0.000353 | 0.000525 | 0.000813 | 0.001248 | 0.000785 | 0.000537 |
Lipid Particles | 0.000258 | 0.000691 | 0.000792 | 0.002655 | 0.000249 | 0.000218 | 0.005764 | 0.000374 | 0.000253 | 0.000378 | 0.001589 | 0.000556 |
Mitochondria | 0.000406 | 0.000739 | 0.000300 | 0.000125 | 0.000249 | 0.000474 | 0.000216 | 0.000565 | 0.000323 | 0.000564 | 0.000286 | 0.000529 |
Mitotic Spindle | 0.001681 | 0.006806 | 0.005957 | 0.000761 | 0.001710 | 0.006863 | 0.005104 | 0.007884 | 0.001696 | 0.006844 | 0.005820 | 0.007313 |
None | 0.005553 | 0.007656 | 0.005883 | 0.003063 | 0.006154 | 0.007385 | 0.012420 | 0.006106 | 0.005869 | 0.007477 | 0.006932 | 0.005862 |
Nuclear Periphery | 0.000078 | 0.000107 | 0.000255 | 0.000434 | 0.000072 | 0.000066 | 0.000853 | 0.000195 | 0.000075 | 0.000080 | 0.000351 | 0.000214 |
Nuclear Periphery Foci | 0.003759 | 0.004755 | 0.001019 | 0.008793 | 0.001442 | 0.001515 | 0.006546 | 0.002616 | 0.002540 | 0.002615 | 0.001906 | 0.003110 |
Nucleolus | 0.000137 | 0.000694 | 0.000114 | 0.000129 | 0.000099 | 0.000114 | 0.000387 | 0.000135 | 0.000117 | 0.000311 | 0.000158 | 0.000135 |
Nucleus | 0.071669 | 0.066012 | 0.156253* | 0.345464* | 0.077260 | 0.063535 | 0.243077* | 0.103834 | 0.074610 | 0.064376 | 0.170184* | 0.123180 |
Peroxisomes | 0.000142 | 0.001141 | 0.000419 | 0.000074 | 0.000140 | 0.000300 | 0.001507 | 0.000074 | 0.000141 | 0.000586 | 0.000593 | 0.000074 |
Vacuole | 0.019802 | 0.019266 | 0.038200 | 0.188908 | 0.017777 | 0.015345 | 0.159193 | 0.046012 | 0.018737 | 0.016676 | 0.057613 | 0.057452 |
Vacuole Periphery | 0.000884 | 0.000807 | 0.000199 | 0.000396 | 0.000244 | 0.000310 | 0.000264 | 0.000981 | 0.000547 | 0.000479 | 0.000210 | 0.000935 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.14 | 0.13 | -5.71 | -5.87 | -5.89 | -2.03 | -10.19 | -7.06 | -0.64 | 7.41 | -1.05 | -2.22 | -3.66 | -2.09 | -0.12 |
Bud Neck | -0.33 | -5.91 | -6.04 | -6.04 | -5.17 | -0.66 | -11.12 | -5.97 | -4.51 | 7.88 | -0.89 | -11.16 | -8.23 | -7.48 | -0.48 |
Bud Site | -1.51 | -0.34 | -6.01 | -4.11 | -5.93 | -1.95 | -6.92 | -4.18 | -0.88 | 4.13 | -2.44 | -2.56 | -5.98 | -1.71 | -3.51 |
Cell Periphery | -0.28 | 0.11 | -7.38 | -7.36 | -7.38 | -0.37 | -13.04 | -10.11 | -9.97 | 6.65 | 0.60 | -10.14 | -10.84 | -10.98 | -7.23 |
Cytoplasm | 2.04 | 23.82 | 19.90 | 19.16 | 13.85 | -1.99 | 28.10 | 15.15 | 16.88 | -18.63 | -0.22 | 33.10 | 19.78 | 19.79 | -4.35 |
Cytoplasmic Foci | -0.62 | 10.99 | -0.52 | -0.09 | -6.46 | 3.33 | 5.67 | 8.41 | 6.03 | 1.72 | 2.09 | 15.57 | 8.36 | 6.71 | -7.31 |
Eisosomes | -1.95 | -1.04 | -6.88 | -4.95 | -6.68 | -0.04 | -8.57 | -3.94 | -3.80 | 7.92 | -1.39 | -7.92 | -5.46 | -3.16 | 3.47 |
Endoplasmic Reticulum | 0.40 | 4.83 | 2.49 | 0.78 | -2.98 | 3.46 | -2.51 | 1.30 | -4.33 | 2.64 | 2.57 | -0.49 | 3.75 | 0.00 | 2.29 |
Endosome | -2.12 | 2.89 | -1.43 | 0.97 | -3.03 | -1.14 | -8.74 | -1.55 | -0.98 | 2.49 | -2.09 | -0.45 | -1.30 | -0.26 | -1.17 |
Golgi | -1.81 | 0.07 | -1.80 | 1.28 | -0.97 | 0.08 | 1.49 | 1.50 | 2.03 | 0.18 | -0.95 | 1.04 | 1.48 | 2.68 | 0.16 |
Lipid Particles | -2.86 | -2.30 | -7.08 | -6.37 | -6.15 | 0.45 | -3.32 | -6.63 | -6.88 | 2.70 | -2.32 | -3.93 | -9.14 | -7.19 | -0.12 |
Mitochondria | -0.63 | 0.69 | 1.51 | 1.46 | 2.08 | -0.99 | 0.18 | -0.41 | 0.36 | -0.52 | -0.99 | 0.53 | -0.02 | 0.74 | -0.32 |
Mitotic Spindle | -2.19 | -4.09 | -0.64 | 1.93 | 3.81 | -3.03 | -2.37 | -4.86 | -1.52 | -1.60 | -3.78 | -4.72 | -5.08 | -1.52 | -1.35 |
None | -1.77 | 0.32 | 5.71 | 5.66 | 8.74 | -1.24 | -0.18 | 1.00 | 3.06 | 0.49 | -2.26 | 0.50 | 1.34 | 3.88 | 0.78 |
Nuclear Periphery | -1.95 | -21.81 | -8.03 | -7.45 | -3.77 | -0.72 | -12.95 | -12.52 | -12.09 | 9.63 | -1.94 | -24.68 | -14.40 | -12.85 | 7.59 |
Nuclear Periphery Foci | -1.58 | 8.48 | -4.56 | -2.97 | -7.05 | -1.18 | -8.61 | -5.39 | -4.44 | 6.40 | -0.84 | 1.61 | -2.79 | -1.42 | -4.19 |
Nucleolus | -1.28 | -0.04 | 0.27 | 1.32 | 1.21 | -0.71 | -7.40 | -1.99 | -0.87 | 4.80 | -1.28 | -1.96 | -0.42 | 1.20 | 2.13 |
Nucleus | 1.09 | -25.62 | -7.19 | -7.36 | -2.19 | 3.84 | -15.34 | -9.41 | -12.66 | 10.67 | 3.53 | -30.62 | -12.32 | -14.44 | 11.44 |
Peroxisomes | -1.86 | -1.15 | 0.31 | 1.91 | 1.33 | -1.75 | -1.08 | 1.94 | 2.86 | 1.26 | -2.30 | -1.55 | 1.99 | 2.71 | 2.03 |
Vacuole | -1.11 | -15.98 | -10.64 | -10.54 | -8.62 | 0.15 | -17.05 | -14.12 | -14.32 | 10.58 | -0.38 | -22.53 | -16.51 | -16.44 | -1.33 |
Vacuole Periphery | 0.23 | 1.75 | 1.64 | 1.12 | -0.82 | -0.42 | 0.45 | -0.95 | -0.79 | -1.11 | 0.38 | 1.92 | 0.06 | -0.24 | -1.30 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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